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Studies on the pathogenic mechanisms of fulminating tree wilts

Research Project

Project/Area Number 18208015
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field 林学・森林工学
Research InstitutionKyoto University

Principal Investigator

FUTAI Kazuyoshi  Kyoto University, 大学院・農学研究科, 教授 (50165445)

Co-Investigator(Kenkyū-buntansha) AZUMA Junnichi  京都大学, 農学研究科, 教授 (80115782)
YAMADA Toshihiro  京都大学, 農学生命科学研究科, 教授 (30332571)
UEHARA Taketo  農業・食品産業技術総合研究機構, 北海道農業研究センター, 主任研究員 (30355458)
Project Period (FY) 2006 – 2008
Project Status Completed (Fiscal Year 2008)
Budget Amount *help
¥47,450,000 (Direct Cost: ¥36,500,000、Indirect Cost: ¥10,950,000)
Fiscal Year 2008: ¥8,580,000 (Direct Cost: ¥6,600,000、Indirect Cost: ¥1,980,000)
Fiscal Year 2007: ¥22,230,000 (Direct Cost: ¥17,100,000、Indirect Cost: ¥5,130,000)
Fiscal Year 2006: ¥16,640,000 (Direct Cost: ¥12,800,000、Indirect Cost: ¥3,840,000)
Keywords劇症型萎凋病 / マツ枯れ / ナラ枯れ / 防御関連物質 / 病原性決定因子 / 媒介昆虫 / 亜社会性行動 / 網羅的分子同定 / 線虫体表タンパク質 / 生物防除 / 坑道内微生物群集 / 劇症型森林流行病 / 弱病原性系統 / 体表面タンパク / カジノナガキクイムシ / 防御応答 / 酵母類 / 防御関連遺伝子 / フェノール性物質 / 潜在感染木 / 寄主応答
Research Abstract

マツ枯れに関しては病原体の純系化に成功し、これにより精度の高い、かつ再現性のある分子生物学的研究が可能になった。さらに、病原線虫感染に始まる寄主-寄生者間の分子応答に関しては、宿主樹体内で異常に増加生成される線虫表皮タンパク質を発見し、その化学的同定に成功したが、寄主の感染応答遺伝子については現在も研究を継続中である。
一方、ナラ枯れに関しては、媒介昆虫の樹体内における亜社会性行動の実態や、この媒介昆虫と関連する菌類の生態や、坑道内での群集構造、さらには病原菌感染後の寄主体内における二次代謝産物の動態などを研究し、一定の成果を得た。さらに、本病を生物学的に防除するために天敵微生物の探索を実施し、これまでにいくつかの候補微生物を得る事に成功した。
以上のように、二つの劇症型森林萎凋病の防除法確立のために必要な基礎知見を明らかにする事に成功しており、今後の未解決課題の研究遂行も併せて、これら流行病の防除法確立に貢献することが可能となろう。

Report

(4 results)
  • 2008 Annual Research Report   Final Research Report ( PDF )
  • 2007 Annual Research Report
  • 2006 Annual Research Report
  • Research Products

    (42 results)

All 2010 2009 2008 2007 2006

All Journal Article (18 results) (of which Peer Reviewed: 12 results) Presentation (17 results) Book (7 results)

  • [Journal Article] Surface coat proteins of the pine wood nematode2009

    • Author(s)
      Shinya, R., Takeuchi, Y., Miura, N., Kuroda, K., Ueda, M., Futai, K.
    • Journal Title

      Bursaphelenchus xylophilus: profiles of stage and isolate-specific characters. Nematology 11

      Pages: 429-438

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Diagnosis and quantification of the pine wood nematode2009

    • Author(s)
      Takeuchi, Y., Futai, K.
    • Journal Title

      Bursaphelenchus xylophilus (Steiner and Buhrer), in wood of Pinus thunbergii with real-time PCR. Nematological Research 39

      Pages: 9-16

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Host selection by Platypus quercivorus (Coleoptera: Platypodidae) before and after flying to trees2009

    • Author(s)
      Yamasaki, M., Futai, K.
    • Journal Title

      Applied Entomology and Zoology 43(2)

      Pages: 249-257

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Surface coat proteins of the pine wood nematode, Bursaphelenchus xylophilus : profiles of stage and isolate specific characters2009

    • Author(s)
      Ryoji Shinya, Yuko Takeuchi, Natsuko Miura, Koichi Kuroda, Mitsuyoshi Ueda, Kazuyoshi Futai
    • Journal Title

      Nematology 11

      Pages: 429-438

    • NAID

      120001759397

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A technique for separating the developmental stages of the propagative form of the pine wood nematode(Bursaphelenchus xylophilus)2009

    • Author(s)
      Ryoji Shinya, Yuko Takeuchi, Kazuyoshi Futai
    • Journal Title

      Nematology 11

      Pages: 305-307

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Diagnosis and quantification of the pine wood nematode, Bursaphelenchus xylophilus(Steiner and Buhrer), in wood of Pinus thunbergii with real-time PCR2009

    • Author(s)
      Yuko Takeuchi, Kazuyoshi Futai
    • Journal Title

      Nematological Research 39

      Pages: 9-16

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Inhibitory effect of endophytic fungi in Quercus serrata on Raffaelea quercivora.2008

    • Author(s)
      Kawai, A., Takeuchi, Y., Azuma, J., Futai, K.
    • Journal Title

      Tree and Forest Health 12(3)

      Pages: 135-136

    • NAID

      110007595143

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Candida kashinagacola sp.nov., C.pseudovanderkliftii sp.nov.and C.vanderkliftii sp.nov., three new yeasts from ambrosia beetle-associated sources2008

    • Author(s)
      Rikiya Endoh, Motofumi Suzuki, Yoshimi Benno, Kazuyoshi Futai
    • Journal Title

      Antonie Van Leeuwenhoek 94

      Pages: 389-402

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Host selection by Platypus quercivorus(Murayama)(Coleoptera : Platypodidae)before and after flying to trees2008

    • Author(s)
      Michimasa Yamasaki, Kazuyoshi Futai
    • Journal Title

      Applied Entomology and Zoology 43

      Pages: 249-257

    • NAID

      110006684227

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Polymorphism of Japanese isolates of the pinewood nematode, Bursaphelenchus xylophilus, at heat-shock protein 70A locus and the field detection of polymorphic populations2007

    • Author(s)
      Takemoto, S., Futai, K.
    • Journal Title

      Applied Entomology and Zoology 42

      Pages: 247-253

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A virulent isolate of the pinewood nematode Bursaplhelenchus xylophilus, survives 7 months in asymptomatic hostseedlings2007

    • Author(s)
      Takeuchi, Y., Futai, K.
    • Journal Title

      Forest Pathology 37

      Pages: 289-291

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A low Platypus quercivorus hole density does not necessarily indicate a smallflying population2007

    • Author(s)
      Yamasaki, M., Iwatake, A., Futai, K.
    • Journal Title

      Japanese Forest Research 12

      Pages: 384-387

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Asymptomatic carrier trees in pine stands naturally infected with Bursaphelenchus xylophilus2007

    • Author(s)
      Takeuchi, Y., Futai, K.
    • Journal Title

      Nematology (in press)

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Polymorphism of Japanese sisolates of the pinewood nematode, Bursaphelenchus xylophilus, at heat-shock protein 70A and the field detection of polymorphic populations.2007

    • Author(s)
      Takemoto, S., Futai, K.
    • Journal Title

      Applied Entomology and Zoology 42・2(in press)

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Cohabitation of the pinewood nematode and fungal species in pine trees inoculated with Bursaphelenchus xylophilus2007

    • Author(s)
      Sriwati, R., Takemoto, S., Futai.K.
    • Journal Title

      Nematology 9・1

      Pages: 77-86

    • Related Report
      2006 Annual Research Report
  • [Journal Article] How different is induced host resistance against the pine wood nematode, Bursaphelenchus xylophiluls, by two avirulent microbes?2006

    • Author(s)
      Takeuchi, Y., Kanzaki, N., Futai, K.
    • Journal Title

      Nematology 8・3

      Pages: 435-442

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Volatile compounds in pine stands suffering from pine wilt disease : qualitative and quantitative evaluation2006

    • Author(s)
      Takeuchi, Y., Kanzaki, N., Futai, K.
    • Journal Title

      Nematology 8・6

      Pages: 869-879

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Comparative studies between Portuguese and Japanese isolates of the pinewood nematode, Bursaphelenchus xylophilus2006

    • Author(s)
      Mota, M.M, Takemoto, S., Takeuchi, Y., Hara, N.
    • Journal Title

      Journal of Nematology 38・4

      Pages: 429-433

    • Related Report
      2006 Annual Research Report
  • [Presentation] カシノナガキクイムシは共生菌類を"播種"するのか?2010

    • Author(s)
      遠藤力也・二井一禎
    • Organizer
      第121回日本森林学会大会
    • Place of Presentation
      筑波大学、茨城
    • Year and Date
      2010-04-04
    • Related Report
      2008 Annual Research Report
  • [Presentation] The role of male and female of ambrosia beetle, Platypus quercivorus in its subsocial system2010

    • Author(s)
      Hagus Tarno, Hongye Qi, Michimasa Yamasaki, Masahide Kobayashi, Kazuyoshi Futai
    • Organizer
      第121回日本森林学会大会
    • Place of Presentation
      筑波大学、茨城
    • Year and Date
      2010-04-03
    • Related Report
      2008 Annual Research Report
  • [Presentation] カシノナガキクイムシ幼虫から分離した微生物の昆虫病原性2010

    • Author(s)
      斉宏業・王建国・竹内祐子・遠藤力也・ハグス タルノ・二井一禎
    • Organizer
      第121回日本森林学会大会
    • Place of Presentation
      筑波大学、茨城
    • Year and Date
      2010-04-03
    • Related Report
      2008 Annual Research Report
  • [Presentation] マツ感染時におけるマツノザイセンチュウ表面タンパク質のフォーカスドプロテオーム解析2009

    • Author(s)
      新屋良治・森坂裕信・竹内祐子・植田充美・二井一禎
    • Organizer
      日本線虫学会第17回大会
    • Place of Presentation
      崇城大学市民ホール、熊本
    • Year and Date
      2009-09-04
    • Related Report
      2008 Annual Research Report
  • [Presentation] マツノザイセンチュウ同一系統内の病原力の多様性2009

    • Author(s)
      市村慶太・新屋良治・竹本周平・竹内祐子・二井一禎
    • Organizer
      日本線虫学会第17回大会
    • Place of Presentation
      崇城大学市民ホール、熊本
    • Year and Date
      2009-09-04
    • Related Report
      2008 Annual Research Report
  • [Presentation] 食植性昆虫ハンノキハムシおよびヤマハンノキの葉から分離された酵母様菌類2009

    • Author(s)
      遠藤力也・鈴木基文・竹内祐子・二井一禎
    • Organizer
      日本菌学会大会
    • Place of Presentation
      鳥取
    • Year and Date
      2009-08-20
    • Related Report
      2008 Annual Research Report
  • [Presentation] Genetic diversity in pathogenicity among isolates of the pine wood nematode, Bursaphelenchus xylophilus2009

    • Author(s)
      Keita Ichimura, Ryoji Shinya, Shuhei Takemoto, Yuko Takeuchi, Kazuyoshi Futai
    • Organizer
      IUFRO 2009 International symposium on pine wilt disease
    • Place of Presentation
      南京林業大学、中国
    • Year and Date
      2009-07-22
    • Related Report
      2008 Annual Research Report
  • [Presentation] Surface coat proteins of the pine wood nematode, Bursaphelenchus xylophilus2009

    • Author(s)
      Ryoji Shinya, Yuko Takeuchi, Hironobu Morisaka, Mitsuyoshi Ueda, Kazuyoshi Futai
    • Organizer
      IUFRO 2009 International symposium on pine wilt disease
    • Place of Presentation
      南京林業大学、中国
    • Year and Date
      2009-07-22
    • Related Report
      2008 Annual Research Report
  • [Presentation] マツノザイセンチュウ表面タンパク質のフォーカスドプロテオーム解析2009

    • Author(s)
      新屋良治・森坂裕信・竹内祐子・植田充美・二井一禎
    • Organizer
      日本農芸化学会2009年度大会
    • Place of Presentation
      福岡国際会議場、福岡
    • Year and Date
      2009-03-29
    • Related Report
      2008 Annual Research Report
  • [Presentation] 養菌性キクイムシと手を組んだ酵母たち2009

    • Author(s)
      遠藤力也・鈴木基文・辨野義己・二井一禎
    • Organizer
      第120回日本森林学会大会
    • Place of Presentation
      京都大学、京都
    • Year and Date
      2009-03-27
    • Related Report
      2008 Annual Research Report
  • [Presentation] Two types of frass are produced by adults and larvae of Platypus quercivorus2009

    • Author(s)
      Hatus Tarno, Hongye Qi, Rikiya Endoh, Masahide Kobayashi, Kazuyoshi Futai
    • Organizer
      第120回日本森林学会大会
    • Place of Presentation
      京都大学、京都
    • Year and Date
      2009-03-27
    • Related Report
      2008 Annual Research Report
  • [Presentation] 養菌性キクイムシと手を組んだ酵母たち2009

    • Author(s)
      遠藤力也・鈴木基文・辨野義己・二井一禎
    • Organizer
      第120回日本森林学会大会
    • Place of Presentation
      京都市
    • Related Report
      2008 Final Research Report
  • [Presentation] マツノザイセンチュウ表面タンパク質の性質2008

    • Author(s)
      新屋良治・竹内祐子・植田充美・二井一禎
    • Organizer
      日本線虫学会第16回大会
    • Place of Presentation
      文部科学省研究交流センター、つくば
    • Year and Date
      2008-09-19
    • Related Report
      2008 Annual Research Report
  • [Presentation] Steinernema 属線虫2種を用いたカシノナガキクイムシの防除法の検討2008

    • Author(s)
      真田陽平・藤本岳人・Hagus Tarno・二井一禎
    • Organizer
      日本線虫学会第16回大会
    • Place of Presentation
      文部科学省研究交流センター、つくば
    • Year and Date
      2008-09-19
    • Related Report
      2008 Annual Research Report
  • [Presentation] Steinernema属線虫2種を用いたカシノナガキクイムシの防除法の検討2008

    • Author(s)
      真田陽平・藤本岳人・Hagus Tarno・二井一禎
    • Organizer
      日本線虫学会第16回大会
    • Place of Presentation
      茨城県つくば市
    • Related Report
      2008 Final Research Report
  • [Presentation] マツノザイセンチュウ表面タンパク質の性質2008

    • Author(s)
      新屋良治・竹内祐子・植田充美・二井一禎
    • Organizer
      日本線虫学会第16回大会
    • Place of Presentation
      茨城県つくば市
    • Related Report
      2008 Final Research Report
  • [Presentation] クロマツ実生苗における菌根菌の初期遷移2007

    • Author(s)
      二井一禎、山本智則、谷口武士
    • Organizer
      日本森林学会
    • Place of Presentation
      九州大学
    • Year and Date
      2007-04-03
    • Related Report
      2007 Annual Research Report
  • [Book] Pine Wilt Disease and the Pine-wood nematode, Bursaphelenchus xylophilus: Integrated Management of Fruit Crops and Forest Nematodes (Ciancio, A. and Mukerji, K. G. eds.)2009

    • Author(s)
      Mota, M. M., Futai, K., Vieira, P.
    • Total Pages
      346
    • Publisher
      Springer
    • Related Report
      2008 Final Research Report
  • [Book] 植物と菌根菌.寄生と共生(石橋、名和編)2008

    • Author(s)
      二井一禎
    • Publisher
      東海大学出版会
    • Related Report
      2008 Final Research Report
  • [Book] 共生微生物の世界.微生物機能の開発(植田編)2008

    • Author(s)
      二井一禎
    • Publisher
      京都大学学術出版会
    • Related Report
      2008 Final Research Report
  • [Book] Mycorrhizae : Sustainable Agriculture and Forestry2008

    • Author(s)
      Siddiqui, Z. A., Akhtar, M. S., Futai, K. eds.
    • Total Pages
      359
    • Publisher
      Springer
    • Related Report
      2008 Final Research Report
  • [Book] Pine Wilt Disease2008

    • Author(s)
      Zhao, B. G., Futai, K., Sutherland, J. R., Takeuchi, Y. eds.
    • Total Pages
      459
    • Publisher
      Springer
    • Related Report
      2008 Final Research Report
  • [Book] Biology and microbial inter-relationships: Pine Wilt Disease: A Worldwide Threat to Forest Ecosystems (Mota, M. M. and Vieira, P. eds)2008

    • Author(s)
      Futai, K., Mota, M. M.
    • Total Pages
      405
    • Publisher
      Springer
    • Related Report
      2008 Final Research Report
  • [Book] Pine Wilt Disease2008

    • Author(s)
      Bo Guang Zhao, Kazuyoshi Futai, Jack R.Sutherland, Yuko Takeuchi
    • Total Pages
      459
    • Publisher
      Springer
    • Related Report
      2008 Annual Research Report

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Published: 2006-04-01   Modified: 2016-04-21  

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