Studies on the pathogenic mechanisms of fulminating tree wilts

• Project/Area Number: 18208015
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: 林学・森林工学
• Research Institution: Kyoto University
• Principal Investigator: FUTAI Kazuyoshi Kyoto University, 大学院・農学研究科, 教授 (50165445)
• Co-Investigator(Kenkyū-buntansha): AZUMA Junnichi 京都大学, 農学研究科, 教授 (80115782) ; YAMADA Toshihiro 京都大学, 農学生命科学研究科, 教授 (30332571) ; UEHARA Taketo 農業・食品産業技術総合研究機構, 北海道農業研究センター, 主任研究員 (30355458)
• Project Period (FY): 2006 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥47,450,000 (Direct Cost: ¥36,500,000、Indirect Cost: ¥10,950,000); Fiscal Year 2008: ¥8,580,000 (Direct Cost: ¥6,600,000、Indirect Cost: ¥1,980,000); Fiscal Year 2007: ¥22,230,000 (Direct Cost: ¥17,100,000、Indirect Cost: ¥5,130,000); Fiscal Year 2006: ¥16,640,000 (Direct Cost: ¥12,800,000、Indirect Cost: ¥3,840,000)
• Keywords: 劇症型萎凋病 / マツ枯れ / ナラ枯れ / 防御関連物質 / 病原性決定因子 / 媒介昆虫 / 亜社会性行動 / 網羅的分子同定 / 線虫体表タンパク質 / 生物防除 / 坑道内微生物群集 / 劇症型森林流行病 / 弱病原性系統 / 体表面タンパク / カジノナガキクイムシ / 防御応答 / 酵母類 / 防御関連遺伝子 / フェノール性物質 / 潜在感染木 / 寄主応答

Research Abstract

マツ枯れに関しては病原体の純系化に成功し、これにより精度の高い、かつ再現性のある分子生物学的研究が可能になった。さらに、病原線虫感染に始まる寄主-寄生者間の分子応答に関しては、宿主樹体内で異常に増加生成される線虫表皮タンパク質を発見し、その化学的同定に成功したが、寄主の感染応答遺伝子については現在も研究を継続中である。
一方、ナラ枯れに関しては、媒介昆虫の樹体内における亜社会性行動の実態や、この媒介昆虫と関連する菌類の生態や、坑道内での群集構造、さらには病原菌感染後の寄主体内における二次代謝産物の動態などを研究し、一定の成果を得た。さらに、本病を生物学的に防除するために天敵微生物の探索を実施し、これまでにいくつかの候補微生物を得る事に成功した。
以上のように、二つの劇症型森林萎凋病の防除法確立のために必要な基礎知見を明らかにする事に成功しており、今後の未解決課題の研究遂行も併せて、これら流行病の防除法確立に貢献することが可能となろう。

Research Products

• [Journal Article] Surface coat proteins of the pine wood nematode (2009)
  • Author(s): Shinya, R., Takeuchi, Y., Miura, N., Kuroda, K., Ueda, M., Futai, K.
  • Journal Title: Bursaphelenchus xylophilus: profiles of stage and isolate-specific characters. Nematology 11 Pages: 429-438
  • Peer Reviewed
• [Journal Article] Diagnosis and quantification of the pine wood nematode (2009)
  • Author(s): Takeuchi, Y., Futai, K.
  • Journal Title: Bursaphelenchus xylophilus (Steiner and Buhrer), in wood of Pinus thunbergii with real-time PCR. Nematological Research 39 Pages: 9-16
  • Peer Reviewed
• [Journal Article] Host selection by Platypus quercivorus (Coleoptera: Platypodidae) before and after flying to trees (2009)
  • Author(s): Yamasaki, M., Futai, K.
  • Journal Title: Applied Entomology and Zoology 43(2) Pages: 249-257
  • Peer Reviewed
• [Journal Article] Surface coat proteins of the pine wood nematode, Bursaphelenchus xylophilus : profiles of stage and isolate specific characters (2009)
  • Author(s): Ryoji Shinya, Yuko Takeuchi, Natsuko Miura, Koichi Kuroda, Mitsuyoshi Ueda, Kazuyoshi Futai
  • Journal Title: Nematology 11 Pages: 429-438
  • NAID: 120001759397
  • Peer Reviewed
• [Journal Article] A technique for separating the developmental stages of the propagative form of the pine wood nematode(Bursaphelenchus xylophilus) (2009)
  • Author(s): Ryoji Shinya, Yuko Takeuchi, Kazuyoshi Futai
  • Journal Title: Nematology 11 Pages: 305-307
  • Peer Reviewed
• [Journal Article] Diagnosis and quantification of the pine wood nematode, Bursaphelenchus xylophilus(Steiner and Buhrer), in wood of Pinus thunbergii with real-time PCR (2009)
  • Author(s): Yuko Takeuchi, Kazuyoshi Futai
  • Journal Title: Nematological Research 39 Pages: 9-16
  • Peer Reviewed
• [Journal Article] Inhibitory effect of endophytic fungi in Quercus serrata on Raffaelea quercivora. (2008)
  • Author(s): Kawai, A., Takeuchi, Y., Azuma, J., Futai, K.
  • Journal Title: Tree and Forest Health 12(3) Pages: 135-136
  • NAID: 110007595143
  • Peer Reviewed
• [Journal Article] Candida kashinagacola sp.nov., C.pseudovanderkliftii sp.nov.and C.vanderkliftii sp.nov., three new yeasts from ambrosia beetle-associated sources (2008)
  • Author(s): Rikiya Endoh, Motofumi Suzuki, Yoshimi Benno, Kazuyoshi Futai
  • Journal Title: Antonie Van Leeuwenhoek 94 Pages: 389-402
  • Peer Reviewed
• [Journal Article] Host selection by Platypus quercivorus(Murayama)(Coleoptera : Platypodidae)before and after flying to trees (2008)
  • Author(s): Michimasa Yamasaki, Kazuyoshi Futai
  • Journal Title: Applied Entomology and Zoology 43 Pages: 249-257
  • NAID: 110006684227
  • Peer Reviewed
• [Journal Article] Polymorphism of Japanese isolates of the pinewood nematode, Bursaphelenchus xylophilus, at heat-shock protein 70A locus and the field detection of polymorphic populations (2007)
  • Author(s): Takemoto, S., Futai, K.
  • Journal Title: Applied Entomology and Zoology 42 Pages: 247-253
  • Peer Reviewed
• [Journal Article] A virulent isolate of the pinewood nematode Bursaplhelenchus xylophilus, survives 7 months in asymptomatic hostseedlings (2007)
  • Author(s): Takeuchi, Y., Futai, K.
  • Journal Title: Forest Pathology 37 Pages: 289-291
  • Peer Reviewed
• [Journal Article] A low Platypus quercivorus hole density does not necessarily indicate a smallflying population (2007)
  • Author(s): Yamasaki, M., Iwatake, A., Futai, K.
  • Journal Title: Japanese Forest Research 12 Pages: 384-387
  • Peer Reviewed
• [Journal Article] Asymptomatic carrier trees in pine stands naturally infected with Bursaphelenchus xylophilus (2007)
  • Author(s): Takeuchi, Y., Futai, K.
  • Journal Title: Nematology (in press)
• [Journal Article] Polymorphism of Japanese sisolates of the pinewood nematode, Bursaphelenchus xylophilus, at heat-shock protein 70A and the field detection of polymorphic populations. (2007)
  • Author(s): Takemoto, S., Futai, K.
  • Journal Title: Applied Entomology and Zoology 42・2(in press)
• [Journal Article] Cohabitation of the pinewood nematode and fungal species in pine trees inoculated with Bursaphelenchus xylophilus (2007)
  • Author(s): Sriwati, R., Takemoto, S., Futai.K.
  • Journal Title: Nematology 9・1 Pages: 77-86
• [Journal Article] How different is induced host resistance against the pine wood nematode, Bursaphelenchus xylophiluls, by two avirulent microbes? (2006)
  • Author(s): Takeuchi, Y., Kanzaki, N., Futai, K.
  • Journal Title: Nematology 8・3 Pages: 435-442
• [Journal Article] Volatile compounds in pine stands suffering from pine wilt disease : qualitative and quantitative evaluation (2006)
  • Author(s): Takeuchi, Y., Kanzaki, N., Futai, K.
  • Journal Title: Nematology 8・6 Pages: 869-879
• [Journal Article] Comparative studies between Portuguese and Japanese isolates of the pinewood nematode, Bursaphelenchus xylophilus (2006)
  • Author(s): Mota, M.M, Takemoto, S., Takeuchi, Y., Hara, N.
  • Journal Title: Journal of Nematology 38・4 Pages: 429-433
• [Presentation] カシノナガキクイムシは共生菌類を"播種"するのか? (2010)
  • Author(s): 遠藤力也・二井一禎
  • Organizer: 第121回日本森林学会大会
  • Place of Presentation: 筑波大学、茨城
  • Year and Date: 2010-04-04
• [Presentation] The role of male and female of ambrosia beetle, Platypus quercivorus in its subsocial system (2010)
  • Author(s): Hagus Tarno, Hongye Qi, Michimasa Yamasaki, Masahide Kobayashi, Kazuyoshi Futai
  • Organizer: 第121回日本森林学会大会
  • Place of Presentation: 筑波大学、茨城
  • Year and Date: 2010-04-03
• [Presentation] カシノナガキクイムシ幼虫から分離した微生物の昆虫病原性 (2010)
  • Author(s): 斉宏業・王建国・竹内祐子・遠藤力也・ハグス タルノ・二井一禎
  • Organizer: 第121回日本森林学会大会
  • Place of Presentation: 筑波大学、茨城
  • Year and Date: 2010-04-03
• [Presentation] マツ感染時におけるマツノザイセンチュウ表面タンパク質のフォーカスドプロテオーム解析 (2009)
  • Author(s): 新屋良治・森坂裕信・竹内祐子・植田充美・二井一禎
  • Organizer: 日本線虫学会第17回大会
  • Place of Presentation: 崇城大学市民ホール、熊本
  • Year and Date: 2009-09-04
• [Presentation] マツノザイセンチュウ同一系統内の病原力の多様性 (2009)
  • Author(s): 市村慶太・新屋良治・竹本周平・竹内祐子・二井一禎
  • Organizer: 日本線虫学会第17回大会
  • Place of Presentation: 崇城大学市民ホール、熊本
  • Year and Date: 2009-09-04
• [Presentation] 食植性昆虫ハンノキハムシおよびヤマハンノキの葉から分離された酵母様菌類 (2009)
  • Author(s): 遠藤力也・鈴木基文・竹内祐子・二井一禎
  • Organizer: 日本菌学会大会
  • Place of Presentation: 鳥取
  • Year and Date: 2009-08-20
• [Presentation] Genetic diversity in pathogenicity among isolates of the pine wood nematode, Bursaphelenchus xylophilus (2009)
  • Author(s): Keita Ichimura, Ryoji Shinya, Shuhei Takemoto, Yuko Takeuchi, Kazuyoshi Futai
  • Organizer: IUFRO 2009 International symposium on pine wilt disease
  • Place of Presentation: 南京林業大学、中国
  • Year and Date: 2009-07-22
• [Presentation] Surface coat proteins of the pine wood nematode, Bursaphelenchus xylophilus (2009)
  • Author(s): Ryoji Shinya, Yuko Takeuchi, Hironobu Morisaka, Mitsuyoshi Ueda, Kazuyoshi Futai
  • Organizer: IUFRO 2009 International symposium on pine wilt disease
  • Place of Presentation: 南京林業大学、中国
  • Year and Date: 2009-07-22
• [Presentation] マツノザイセンチュウ表面タンパク質のフォーカスドプロテオーム解析 (2009)
  • Author(s): 新屋良治・森坂裕信・竹内祐子・植田充美・二井一禎
  • Organizer: 日本農芸化学会2009年度大会
  • Place of Presentation: 福岡国際会議場、福岡
  • Year and Date: 2009-03-29
• [Presentation] 養菌性キクイムシと手を組んだ酵母たち (2009)
  • Author(s): 遠藤力也・鈴木基文・辨野義己・二井一禎
  • Organizer: 第120回日本森林学会大会
  • Place of Presentation: 京都大学、京都
  • Year and Date: 2009-03-27
• [Presentation] Two types of frass are produced by adults and larvae of Platypus quercivorus (2009)
  • Author(s): Hatus Tarno, Hongye Qi, Rikiya Endoh, Masahide Kobayashi, Kazuyoshi Futai
  • Organizer: 第120回日本森林学会大会
  • Place of Presentation: 京都大学、京都
  • Year and Date: 2009-03-27
• [Presentation] 養菌性キクイムシと手を組んだ酵母たち (2009)
  • Author(s): 遠藤力也・鈴木基文・辨野義己・二井一禎
  • Organizer: 第120回日本森林学会大会
  • Place of Presentation: 京都市
• [Presentation] マツノザイセンチュウ表面タンパク質の性質 (2008)
  • Author(s): 新屋良治・竹内祐子・植田充美・二井一禎
  • Organizer: 日本線虫学会第16回大会
  • Place of Presentation: 文部科学省研究交流センター、つくば
  • Year and Date: 2008-09-19
• [Presentation] Steinernema 属線虫2種を用いたカシノナガキクイムシの防除法の検討 (2008)
  • Author(s): 真田陽平・藤本岳人・Hagus Tarno・二井一禎
  • Organizer: 日本線虫学会第16回大会
  • Place of Presentation: 文部科学省研究交流センター、つくば
  • Year and Date: 2008-09-19
• [Presentation] Steinernema属線虫2種を用いたカシノナガキクイムシの防除法の検討 (2008)
  • Author(s): 真田陽平・藤本岳人・Hagus Tarno・二井一禎
  • Organizer: 日本線虫学会第16回大会
  • Place of Presentation: 茨城県つくば市
• [Presentation] マツノザイセンチュウ表面タンパク質の性質 (2008)
  • Author(s): 新屋良治・竹内祐子・植田充美・二井一禎
  • Organizer: 日本線虫学会第16回大会
  • Place of Presentation: 茨城県つくば市
• [Presentation] クロマツ実生苗における菌根菌の初期遷移 (2007)
  • Author(s): 二井一禎、山本智則、谷口武士
  • Organizer: 日本森林学会
  • Place of Presentation: 九州大学
  • Year and Date: 2007-04-03
• [Book] Pine Wilt Disease and the Pine-wood nematode, Bursaphelenchus xylophilus: Integrated Management of Fruit Crops and Forest Nematodes (Ciancio, A. and Mukerji, K. G. eds.) (2009)
  • Author(s): Mota, M. M., Futai, K., Vieira, P.
  • Total Pages: 346
  • Publisher: Springer
• [Book] 植物と菌根菌.寄生と共生(石橋、名和編) (2008)
  • Author(s): 二井一禎
  • Publisher: 東海大学出版会
• [Book] 共生微生物の世界.微生物機能の開発(植田編) (2008)
  • Author(s): 二井一禎
  • Publisher: 京都大学学術出版会
• [Book] Mycorrhizae : Sustainable Agriculture and Forestry (2008)
  • Author(s): Siddiqui, Z. A., Akhtar, M. S., Futai, K. eds.
  • Total Pages: 359
  • Publisher: Springer
• [Book] Pine Wilt Disease (2008)
  • Author(s): Zhao, B. G., Futai, K., Sutherland, J. R., Takeuchi, Y. eds.
  • Total Pages: 459
  • Publisher: Springer
• [Book] Biology and microbial inter-relationships: Pine Wilt Disease: A Worldwide Threat to Forest Ecosystems (Mota, M. M. and Vieira, P. eds) (2008)
  • Author(s): Futai, K., Mota, M. M.
  • Total Pages: 405
  • Publisher: Springer
• [Book] Pine Wilt Disease (2008)
  • Author(s): Bo Guang Zhao, Kazuyoshi Futai, Jack R.Sutherland, Yuko Takeuchi
  • Total Pages: 459
  • Publisher: Springer