Studies on mechanism of the regulation of body temperature in chicks during the neonatal stage

• Project/Area Number: 18208023
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Zootechnical science/Grassland science
• Research Institution: Kyushu University
• Principal Investigator: FURUSE Mitsuhiro Kyushu University, 大学院・農学研究院, 教授 (30209176)
• Co-Investigator(Kenkyū-buntansha): TOYOMIZU Masaaki 東北大学, 大学院・農学研究科, 教授 (80180199) ; MURAI Atsushi 名古屋大学, 大学院・生命農学研究科, 准教授 (10313975)
• Project Period (FY): 2006 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥47,970,000 (Direct Cost: ¥36,900,000、Indirect Cost: ¥11,070,000); Fiscal Year 2008: ¥7,410,000 (Direct Cost: ¥5,700,000、Indirect Cost: ¥1,710,000); Fiscal Year 2007: ¥15,860,000 (Direct Cost: ¥12,200,000、Indirect Cost: ¥3,660,000); Fiscal Year 2006: ¥24,700,000 (Direct Cost: ¥19,000,000、Indirect Cost: ¥5,700,000)
• Keywords: 家畜管理 / 飼養管理 / 栄養管理 / 飼養標準 / 環境生理 / アミノ酸 / ペプチド / エネルギー代謝 / CRF / 視床下部-下垂体-副腎皮質軸 / 体温 / 脂肪酸 / ヒナ / 呼吸商 / 基質 / 血管作動性腸ペプチド / グルカゴン様ペプチド-1 / α-メラニン細胞刺激ホルモン / 下垂体アデニル酸シクラーゼ活性化ポリペプチド / 甲状腺刺激ホルモン放出ホルモン / 副腎皮質刺激ホルモン放出ホルモン / 酸素消費量 / 二酸化炭素排出量 / 熱生産量 / ニューロペプチドY / 卵黄 / 肝臓

Research Abstract

ニワトリは早成鳥であるがために家禽として重要な位置を占めてきた。その一方で、恒温動物であり、体温が高いものと認識されてきた。ところが、孵化後しばらくは体温調節がうまく機能せず、短期間の体温維持しかできないために保温が必要となる。この原因を解明するために本研究を遂行し、以下の成果を得た。
多くの神経ペプチドは遊離脂肪酸をエネルギー源として体温調節に働くが、体内の貯蔵脂肪には限りがあるために長期間の体温維持は困難である。また、活動による運動エネルギーも体温維持に貢献することが判明した。行動量に影響を及ぼす栄養素は多く存在することも判明した。
ミトコンドリアの熱産生分子であるニワトリ脱共役タンパク質(avUCP)とミトコンドリア陰イオン交換体(MACP)の一つであるアデニンヌクレオチド輸送体(avANT)の発現は低温暴露しても変化せず、脂肪酸の代謝に関連する酵素の働きでエネルギー産生に傾くことが明らかとなった。
孵化後のエネルギー代謝に関わる残存卵黄の貢献を調べたところ、その除去により肝臓における脂肪酸分解と糖新生に関わる遺伝子の発現が高まった。残存卵黄は、脂肪酸供給ならびに糖新生に貢献することが判明した。

Research Products

• [Journal Article] Putative neuroprotective role of centrally administered corticotropin-releasing factor in low-temperature exposed neonatal chicks (2009)
  • Author(s): Mujahid, A. and Furuse, M.
  • Journal Title: Neuroscience 158(4) Pages: 1571-1576
  • Peer Reviewed
• [Journal Article] Oxidative damage in different tissues of neonatal chicks exposed to low environmental temperature (2009)
  • Author(s): Mujahid, A. and Furuse, M.
  • Journal Title: Comparative Biochemistry and Physiology A, Molecular and Integrative Physiology 152(4) Pages: 604-608
  • Peer Reviewed
• [Journal Article] Behavioral responses of neonatal chicks exposed to low environmental temperature (2009)
  • Author(s): Mujahid, A. and Furuse, M
  • Journal Title: Poultry Science 88(5) Pages: 917-922
  • Peer Reviewed
• [Journal Article] Central functions of nutrients for the stress response in chicks (2009)
  • Author(s): Yamane, H., Kurauchi, I., Denbow, D.M. and Furuse, M.
  • Journal Title: Asian-Australasian Journal of Animal Sciece 22(2) Pages: 296-304
  • Peer Reviewed
• [Journal Article] Screening dipeptides having central functions for excitation and sedation (2009)
  • Author(s): Yamane, H., Denbow, D.M. and Furuse, M.
  • Journal Title: Mini-Reviews in Medicinal Chemistry 9(3) Pages: 300-305
  • Peer Reviewed
• [Journal Article] Current knowledge about the roles of ghrelin in regulating food intake and energy balance in birds (2009)
  • Author(s): Kaiya, H., Furuse, M., Miyazato, M. and Kangawa, K.
  • Journal Title: General and Comparative Endocrinology (in press)
  • Peer Reviewed
• [Journal Article] Central L-alanine reduces energy expenditure with a hypnotic effect under an acute stressful condition in neonatal chicks (2009)
  • Author(s): Kurauchi, et al.
  • Journal Title: Amino Acids 36 Pages: 131-135
  • Peer Reviewed
• [Journal Article] Putative neuroprotective role of centrally administered corticotropin-releasing factor in low-temperature exposed neonatal chicks (2009)
  • Author(s): Mujahid, A., Furuse, M.
  • Journal Title: Neuroscience 158 Pages: 1571-1576
  • Peer Reviewed
• [Journal Article] Oxidative damage in different tissues of neonatal chicks exposed to low environmental temperature (2009)
  • Author(s): Mujahid, A., Furuse, M.
  • Journal Title: Comparative Biochemistry and Physiology A, Molecular and Integrative Physiology 152 Pages: 604-608
  • Peer Reviewed
• [Journal Article] Behavioral responses of neonatal chicks exposed to low environmental temperature (2009)
  • Author(s): Mujahid, A., Furuse, M.
  • Journal Title: Poultry Science 88 Pages: 917-922
  • Peer Reviewed
• [Journal Article] Central functions of nutrients for the stress response in chicks (2009)
  • Author(s): Yamane, et al.
  • Journal Title: Asian-Australasian Journal of Animal Science 22 Pages: 296-304
  • Peer Reviewed
• [Journal Article] Screening dipeptides having central functions for excitation and sedation (2009)
  • Author(s): Yamane, et al.
  • Journal Title: Mini-Reviews in Medicinal Chemistry 9 Pages: 300-305
  • Peer Reviewed
• [Journal Article] Cholinergic neurotransmission is not involved in sedation induced by L-proline in neonatal chicks (2009)
  • Author(s): Hamasu, et al.
  • Journal Title: Journal of Animal and Veterinary Advances 8 Pages: 1207-1211
  • Peer Reviewed
• [Journal Article] L-Proline is a sedative regulator of acute stress in the brain of neonatal chicks (2009)
  • Author(s): Hamasu, et al.
  • Journal Title: Amino Acids (印刷中)
  • Peer Reviewed
• [Journal Article] Intracerebroventricular injection of reduced glutathione decreases energy expenditure under a separation stress in neonatal chicks (2009)
  • Author(s): Yamane, et al.
  • Journal Title: Animal Science Journal (印刷中)
  • Peer Reviewed
• [Journal Article] Intracerebroventricular injection of L-aspartic acid and L-asparagine induces sedative effects under an acute stressful condition in neonatal chicks (2009)
  • Author(s): Yamane, et al.
  • Journal Title: Animal Science Journal (印刷中)
  • Peer Reviewed
• [Journal Article] Central L-cysteine induces sleep, but D-cysteine does sleep with abnormal behavior, under an acute stress in neonatal chicks (2009)
  • Author(s): Yamane, et al.
  • Journal Title: Animal Science Journal (印刷中)
  • Peer Reviewed
• [Journal Article] N-Methyl-D-aspartate and α-amino-3-hydroxy-5-methyl-4-isoxazolepropionate receptors involved in the induction of sedative effects under an acute stress in neonatal chicks (2009)
  • Author(s): Yamane, et al.
  • Journal Title: Amino Acids (印刷中)
  • Peer Reviewed
• [Journal Article] Intracerebroventricular injection of L-proline and D-proline induces sedative and hypnotic effects by different mechanisms under an acute stressful condition in chicks (2009)
  • Author(s): Hamasu, et al.
  • Journal Title: Amino Acids (印刷中)
  • Peer Reviewed
• [Journal Article] Central administration of corticotrophin-releasing factor induces thermogenesis by changes in mitochondrial bioenergetics in neonatal chicks (2008)
  • Author(s): Mujahid, A. and Furuse, M.
  • Journal Title: Neuroscience 155(3) Pages: 845-851
  • Peer Reviewed
• [Journal Article] Thermogenesis in neonatal chicks exposed to acute cold stress with or without intracerebroventricular administration of corticotropin-releasing factor (2008)
  • Author(s): Mujahid, A. and Furuse, M.
  • Journal Title: FEBS Letters 582(20) Pages: 3052-3060
  • Peer Reviewed
• [Journal Article] Central administration of corticotropin-releasing factor induces tissue specific oxidative damage in chicks. (2008)
  • Author(s): Mujahid, A. and Furuse, M.
  • Journal Title: Comparative Biochemistry and Physiology A Molecular and Integrative Physiology 151(4) Pages: 664-669
  • Peer Reviewed
• [Journal Article] Relationships between the sedative and hypnotic effects of intracerebroventricular administration of L-serine and its metabolites, pyruvate and the derivative aminoacids contents in the neonatal chicks under acute stressful conditions (2008)
  • Author(s): Asechi, M., et al.
  • Journal Title: Amino Acids 34 Pages: 55-60
  • Peer Reviewed
• [Journal Article] Reverse structure of canosine induced sedative and hynotic effects in the chick under acute stress (2008)
  • Author(s): Tsuneyoshi, Y., et al.
  • Journal Title: Life Sciences 82 Pages: 1065-1069
  • Peer Reviewed
• [Journal Article] Central L-arginine reduced stress responses are mediated by L-ornithine in neonatal chicks (2008)
  • Author(s): Suenaga, R., et al.
  • Journal Title: Amino Acids 35 Pages: 107-113
  • Peer Reviewed
• [Journal Article] Intracerebroventricular injection of L-arginine induces sedative and hypnotic effects under an acute stress in neonatal chicks (2008)
  • Author(s): Suenaga, R., et al.
  • Journal Title: Amino Acids 35 Pages: 139-146
  • Peer Reviewed
• [Journal Article] Central administration of corticotrophin-releasing factor induces thermogenesis by changes in mitochondrial bioenergetics in neonatal chicks (2008)
  • Author(s): Mujahid, A., Furuse, M.
  • Journal Title: Neuroscience 155 Pages: 845-851
  • Peer Reviewed
• [Journal Article] Thermogenesis in neonatal chicks exposed to acute cold stress with or without intracerebroventricular administration of corticotropin-releasing factor (2008)
  • Author(s): Mujahid, A., Furuse, M.
  • Journal Title: FEBS Letters 582 Pages: 3052-3060
  • Peer Reviewed
• [Journal Article] Central administration of corticotropin-releasing factor induces tissue specific oxidative damage in chicks (2008)
  • Author(s): Mujahid, A., Furuse, M.
  • Journal Title: Comparative Biochemistry and Physiology A, Molecular and Integrative Physiology 151 Pages: 664-669
  • Peer Reviewed
• [Journal Article] L-Serine induces sedative-hypnotic effects via GABA_A receptor in neonatal chicks (2008)
  • Author(s): Shigemi, et al.
  • Journal Title: European Journal of Pharmacology 599 Pages: 86-90
  • Peer Reviewed
• [Journal Article] アミノ酸によるストレス制御 (2008)
  • Author(s): 古瀬充宏, 蔵内勇夫, 山根春香
  • Journal Title: 栄養生理研究会報 52 Pages: 31-41
  • NAID: 10021920855
  • Peer Reviewed
• [Journal Article] Behavioral regulators in the brain of neonatal chicks (2007)
  • Author(s): Furuse, M.
  • Journal Title: Animal Science Journal 78(3) Pages: 218-232
  • NAID: 10021224913
  • Peer Reviewed
• [Journal Article] Neuropeptidergic Regulation of Food Intake in the Neonatal Chick (2007)
  • Author(s): Furuse, M., Yamane, H., Tomonaga, S., Tsuneyoshi, Y. and Denbow, D.M.
  • Journal Title: A Review Journal of Poultry Science 44(4) Pages: 349-356
  • Peer Reviewed
• [Journal Article] Regulation of stress responses by peptides and small nitrogenous compounds in neonatal chicks (2007)
  • Author(s): Yamane, H., Denbow, D.M. and Furuse, M.
  • Journal Title: Avian and Poultry Biology Reviews 18(4) Pages: 107-122
  • Peer Reviewed
• [Journal Article] Central administration of vasoactive intestinal peptide and pituitary adenylate cyclase-activatingpolypeptide differently regulates energy metabolism in chicks (2007)
  • Author(s): Tachibana, et. al.
  • Journal Title: Comparative Biochemistry and Physiology A, Molecular and Integrative Physiology 147 Pages: 156-164
  • Peer Reviewed
• [Journal Article] Intracerebroventricular injection of glucagon-like peptide-1 changes lipid metabolism in chicks (2007)
  • Author(s): Tachibana, et. al.
  • Journal Title: Comparative Biochemistry and Physiology A, Molecular and Integrative Physiology 147 Pages: 1104-1108
  • Peer Reviewed
• [Journal Article] Central administration of alpha-melanocyte-stimulating hormone changes lipid metabolism in chicks (2007)
  • Author(s): Tachibana, et. al.
  • Journal Title: Comparative Biochemistry and Physiology A, Molecular and Integrative Physiology 148 Pages: 408-412
  • Peer Reviewed
• [Journal Article] Thyrotropin-releasing hormone increased heat production without the involvement of corticotropin-releasing factor in neonatal chicks (2006)
  • Author(s): Tachibana, T. et al.
  • Journal Title: Pharmacology Biochemistry and Behavior 83(4) Pages: 528-532
• [Journal Article] Intracerebroventricular injection of neuropeptide Y modifies carbohydrate and lipid metabolism in chicks (2006)
  • Author(s): Tachibana, T. et al.
  • Journal Title: Regulatory Peptides 136(1-3) Pages: 1-8
• [Journal Article] Total lipid and triacylglycerol contents in the liver of broiler and layer chickens at embryonic stages and hatch (2006)
  • Author(s): Sato, M. et al.
  • Journal Title: Animal Science Journal 77(5) Pages: 526-531
• [Presentation] 中枢オレキシンが幼雛の行動に及ぼす影響 (2009)
  • Author(s): 片山佐知子, 他
  • Organizer: 日本家禽学会
  • Place of Presentation: 日本大学、藤沢市
  • Year and Date: 2009-03-28
• [Presentation] 孵化直後に残存卵黄を除去したニワトリヒナにおける栄養素代謝の特徴 (2009)
  • Author(s): 村井篤嗣, 他
  • Organizer: 日本家禽学会
  • Place of Presentation: 日本大学、藤沢市
  • Year and Date: 2009-03-28
• [Presentation] 新生ヒナにおける低温暴露による体温調節と基質の酸化 (2009)
  • Author(s): Ahmad Mujahid, 古瀬充宏
  • Organizer: 日本家禽学会
  • Place of Presentation: 日本大学、藤沢市
  • Year and Date: 2009-03-28
• [Presentation] 低温環境馴化時における鶏脱共役タンパク質の発現応答とその機能の解明 (2009)
  • Author(s): 乾恵理子・喜久里 基・松本 祐世・豊水正昭
  • Organizer: 日本畜産学会
• [Presentation] 新生ヒナにおける低温暴露による体温調節と基質の酸化 (2009)
  • Author(s): Ahmad Mujahid・古瀬充宏
  • Organizer: 日本家禽学会
• [Presentation] 孵化直後に残存卵黄を除去したニワトリヒナにおける栄養素代謝の特徴 (2009)
  • Author(s): 村井篤嗣・Bae Hae-Duck・小澤尭・小林慧三・小林美里・堀尾文彦・古瀬充宏
  • Organizer: 日本家禽学会
• [Presentation] ペットにおけるアミノ酸栄養 (2008)
  • Author(s): 古瀬充宏, 他
  • Organizer: 飼料用アミノ酸研究会
  • Place of Presentation: 日本橋公会堂、東京都
  • Year and Date: 2008-11-18
• [Presentation] 興奮性神経伝達物質がストレス状況下のヒナの行動に与える影響-クルクミン酸・アスパラギン酸は鎮静・催眠効果をもたらす- (2008)
  • Author(s): 山根春香, 他
  • Organizer: 家畜栄養生理研究会
  • Place of Presentation: つくば国際センター、つくば市
  • Year and Date: 2008-10-21
• [Presentation] アミノ酸によるストレス制御 (2008)
  • Author(s): 古瀬充宏, 他
  • Organizer: 家畜栄養生理研究会
  • Place of Presentation: つくば国際センター、つくば市
  • Year and Date: 2008-10-21
• [Presentation] ノルエピネフリンがニワトリヒナの脳内摂食調節因子の遺伝子発現に及ぼす影響 (2008)
  • Author(s): 片山佐知子, 他
  • Organizer: 日本獣医学会
  • Place of Presentation: ワールドコンベンションセンターサミット、宮崎市
  • Year and Date: 2008-09-25
• [Presentation] 脳内L-セリンの鎮静・睡眠作用におけるメカニズム解明 (2008)
  • Author(s): 重見和孝, 他
  • Organizer: 日本獣医学会
  • Place of Presentation: ワールドコンベンションセンターサミット、宮崎市
  • Year and Date: 2008-09-25
• [Presentation] 低温暴露による新生ヒナの熱産生反応、酸化的損傷および行動における変化 (2008)
  • Author(s): Mujahid Ahmad・古瀬 充宏
  • Organizer: 日本家禽学会
  • Place of Presentation: 水戸市
  • Year and Date: 2008-03-28
• [Presentation] Thermogenesis and mitochondrial bioenergetics in neonatal chicks exposed to cold stress with or without CRF (2008)
  • Author(s): Mujahid Ahmad・古瀬充宏
  • Organizer: 日本畜産学会
• [Presentation] 低温暴露による新生ヒナの熱産生反応、酸化的損傷および行動における変化 (2008)
  • Author(s): Mujahid Ahmad・古瀬充宏
  • Organizer: 日本家禽学会
• [Book] Central functions of green tea components. In : Handbook of Green Tea and Health Research (Edited by Helen McKinley and Mark Jamieson) (2009)
  • Author(s): Furuse, M., Adachi, N., Tomonaga, S., Yamane, H. and Denbow, D.M.
  • Publisher: NOVA Science Publishers Inc., Hauppauge NY, USA.(in press)
• [Book] Functions of L-pipecolic acid as an L-lysine metabolite in the brain. In : Trends in Brain Mapping Research (Edited by Chen FJ) (2006)
  • Author(s): Furuse, M., Takagi, T., Bungo, T. and Denbow, D.M.
  • Publisher: NOVA Science Publishers Inc., Hauppauge NY, USA
• [Remarks]
  • URL: http://www.brs.kyushu-u.ac.jp/~animals/
• [Remarks]
  • URL: http://www.agri.tohoku.ac.jp/aninutr/
• [Remarks]
  • URL: http://www.agr.nagoya-u.ac.jp/~anutr/
• [Remarks]
  • URL: http://www.brs.kyushu-u.ac.jp/~animals/
• [Remarks]
  • URL: http://www.brs.kyushu-u.ac.jp/~animals/
• [Patent(Industrial Property Rights)] 睡眠誘導剤、ストレス性不眠症改善剤 (2007)
  • Inventor(s): 韓力・速水耕介・古瀬充宏・山根春香
  • Industrial Property Rights Holder: 韓力・速水耕介・古瀬充宏・山根春香
  • Filing Date: 2007-02-02
• [Patent(Industrial Property Rights)] 自発運動促進剤 (2007)
  • Inventor(s): 古瀬充宏・恒吉洋佑・森下幸治
  • Industrial Property Rights Holder: 古瀬充宏・恒吉洋佑・森下幸治
  • Filing Date: 2007-09-06
• [Patent(Industrial Property Rights)] 睡眠誘導剤、ストレス性不眠症改善剤 (2006)
  • Inventor(s): 韓力・速水耕介・富裕孝・古瀬充宏・山根春香
  • Industrial Property Rights Holder: 韓力・速水耕介・富裕孝・古瀬充宏・山根春香
  • Filing Date: 2006-09-01
• [Patent(Industrial Property Rights)] 抗不安剤 (2006)
  • Inventor(s): 韓力・速水耕介・古瀬充宏
  • Industrial Property Rights Holder: 韓力・速水耕介・古瀬充宏
  • Filing Date: 2006-10-24
• [Patent(Industrial Property Rights)] 睡眠誘導剤、ストレス性不眠症改善剤
  • Inventor(s): 韓力・速水耕介・古瀬充宏
  • Industrial Property Rights Holder: 韓力・速水耕介・古瀬充宏