Regulation of germ line function and development by genomic imprinting

• Project/Area Number: 18208024
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied animal science
• Research Institution: Tokyo University of Agriculture
• Principal Investigator: KONO Tomohiro Tokyo University of Agriculture, 応用生物科学部, 教授 (80153485)
• Co-Investigator(Kenkyū-buntansha): SOTOMARU Yusuke 広島大学, 自然科学研究支援開発センター, 准教授 (90309352)
• Project Period (FY): 2006 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥47,710,000 (Direct Cost: ¥36,700,000、Indirect Cost: ¥11,010,000); Fiscal Year 2008: ¥13,910,000 (Direct Cost: ¥10,700,000、Indirect Cost: ¥3,210,000); Fiscal Year 2007: ¥13,910,000 (Direct Cost: ¥10,700,000、Indirect Cost: ¥3,210,000); Fiscal Year 2006: ¥19,890,000 (Direct Cost: ¥15,300,000、Indirect Cost: ¥4,590,000)
• Keywords: ゲノムインプリンティング / 単為発生 / マイクロアレイ / 遺伝子発現

Research Abstract

父性メチル化インプリント制御に焦点を当て研究を進め新規知見を得た。二母性マウスの胎仔および胎盤におけるマイクロアレイ解析を行い、6番および12番染色体の父性メチル化領域のインプリント遺伝子により個体発生が制御されていることを明らかにした。12番染色体の父性メチル化領域の母方発現Gtl2転写産物から生じるmiRNAが個体発生に重要な役割をもつことを初めて明らかにした。マウス1番染色体およびヒト2番染色体で父性発現するインプリント遺伝子Zdbf2を初めて発見した。

Research Products

• [Journal Article] Genetic modification for bimaternal embryo development (2009)
  • Author(s): Kono, T
  • Journal Title: Reproduction, Fertility and Development 21 Pages: 31-36
  • Peer Reviewed
• [Journal Article] Identification of the mouse paternally expressed imprinted gene Zdbf2 on chromosome 1 and its imprinted human homolog ZDBF2 on chromosome 2 (2009)
  • Author(s): Kobayashi, H., Sotomaru, Y., Kono, T. 他7名
  • Journal Title: Genomics 93 Pages: 461-472
  • Peer Reviewed
• [Journal Article] Developmental ability of trophoblast stem cells in uniparental mouse embryos (2009)
  • Author(s): Ogawa, H., Sotomaru, Y., Kono, T. 他9名
  • Journal Title: Placenta 30 Pages: 448-456
  • Peer Reviewed
• [Journal Article] Deletion of Gtl2, imprinted non-coding RNA, with its differentially methylated region induces lethal parent-origindependent defects in mice (2009)
  • Author(s): Takahashi, N., Sotomaru, Y.,Kono, T. 他5名
  • Journal Title: Human Molecular Genetics 18 Pages: 1879-1888
  • Peer Reviewed
• [Journal Article] Defining contributions of paternallymethylated imprinted genes at the IGF2-H19 and DLK1-GTl2 domains tomouse placentation by transcriptomicanalysis (2009)
  • Author(s): Kawahara, M., Kono, T. 他2名
  • Journal Title: Jornal of Biological Chemistry(published online on April 20
  • Peer Reviewed
• [Journal Article] Genetic modification for bimatemal embryo development. (2009)
  • Author(s): Kono, Tomohiro.
  • Journal Title: Reproduction, Fertility and Development 21 Pages: 31-36
  • Peer Reviewed
• [Journal Article] Identification of the mouse paternally expressed imprinted gene Zdbf2 on chromosome 1 and its imprinted human homolog ZDBF2 on chromosome 2. (2009)
  • Author(s): Kobayashi, Hisato., Sotomaru, Yusuke., Kono, Tomohiro., 他7名
  • Journal Title: Genomics 93 Pages: 461-472
  • Peer Reviewed
• [Journal Article] Developmental ability of trophoblast stem cells in uniparental mouse embryos. (2009)
  • Author(s): Ogawa, Hidehiko., Sotomaru, Yusuke., Kono, Tomohiro., 他9名
  • Journal Title: Placenta 30 Pages: 448-456
  • Peer Reviewed
• [Journal Article] Deletion of Gtl2, imprinted non-coding RNA, with its differentially methylated region induces lethal parent-origin-dependent defects in mice. (2009)
  • Author(s): Takahashi, Nozomi., Sotomaru, Yusuke., Kono, Tomohiro., 他5名
  • Journal Title: Human Molecular Genetics 18 Pages: 1879-1888
  • Peer Reviewed
• [Journal Article] Defining contributions of paternally methylated imprinted genes at the IGF2-H19 and DLK1-GTl2 domains to mouse placentation by transcriptomic analysis. (2009)
  • Author(s): Kawahara, Manabu., Kono, Tomohiro., 他2名
  • Journal Title: Journal of Biological Chemistry Published online on April 20
  • NAID: 120006377532
  • Peer Reviewed
• [Journal Article] Protocol for the production of viable bimaternal mouse embryos (2008)
  • Author(s): Kawahara, M., Sotomaru , Y., Kono T. 他5名
  • Journal Title: Nature protocols 3 Pages: 197-209
  • Peer Reviewed
• [Journal Article] Protocol for the production of viable bimatemal mouse embryos. (2008)
  • Author(s): Kawahara, Manabu., Sotomaru, Yusuke., Kono Tomohiro., 他5名
  • Journal Title: Nature protocols 3 Pages: 197-209
  • Peer Reviewed
• [Journal Article] Exploration of the genes responsible for unlimited proliferation of immortalized lung fibroblasts. (2008)
  • Author(s): Hiyama, Keiko. Sotomaru, Yusuke., 他6名
  • Journal Title: Experimental Lung Research 34 Pages: 373-390
  • NAID: 120001092454
  • Peer Reviewed
• [Journal Article] 二母性マウスの開発 (2008)
  • Author(s): 河野 友宏
  • Journal Title: 細胞工学 27 Pages: 812-819
• [Journal Article] ゲノムインプリンティングと二母性マウスの開発 (2008)
  • Author(s): 川原 学, 河野 友宏
  • Journal Title: 化学と生物 46 Pages: 452-459
  • NAID: 10024333917
• [Journal Article] DNA methylation imprints on the IG-DMR of the Dlk1-Gtl2domain in mouse male germline (2007)
  • Author(s): Hiura, H., Kono, T. 他4名
  • Journal Title: FEBS Letters 581 Pages: 1255-1260
  • Peer Reviewed
• [Journal Article] Isolation,characterization,and invitro and in vivo differentiation ofputative thecal stem cells (2007)
  • Author(s): Honda, A., Kono, T., 他10名
  • Journal Title: PNA 104 Pages: 12389-12394
  • Peer Reviewed
• [Journal Article] High-frequency generation of viablemice from engineered bi-maternal embryos (2007)
  • Author(s): Kawahara,M., Kono, T. 他6名
  • Journal Title: Nature biotechnology 25 Pages: 1045-1050
  • Peer Reviewed
• [Journal Article] Long-termeffects of In vitro growth of mouseoocytes on their maturation anddevelopment (2007)
  • Author(s): Obata, Y., Kono,T. 他2名
  • Journal Title: Journal of reproduction and development 53 Pages: 183-1190
  • Peer Reviewed
• [Journal Article] Appropriate expression of imprinted genes on mouse chromosome 12 extends development of bi-maternal embryos to term (2007)
  • Author(s): Kawahara, M., Kono, T. 他2名
  • Journal Title: FEBS Letters 581 Pages: 5178-5184
  • Peer Reviewed
• [Journal Article] DNA methylation imprints on the IG-DMR of the Dlk1-Gt12 domain in mouse male germline (2007)
  • Author(s): Hiura H, Kono T, 他4名
  • Journal Title: FEBS Letters 25・9 Pages: 1255-1260
  • Peer Reviewed
• [Journal Article] Isolation, characterization, and in vitro and in vivo differentiation of putative thecal stem cells (2007)
  • Author(s): Honda A, Kono T, 他10名
  • Journal Title: PNAS 104・30 Pages: 12389-12394
  • Peer Reviewed
• [Journal Article] High frequency generation of viable mice from engineered bi-maternal embryos (2007)
  • Author(s): Kawahara M, Kono T, 他6名
  • Journal Title: Nature Biotechnology 25・9 Pages: 1045-1050
  • Peer Reviewed
• [Journal Article] Long-term effects of in vitro growth of mouse oocytes on their maturation and development (2007)
  • Author(s): Obata Y, Kono T, 他2名
  • Journal Title: Journal of Reproduction and Development 53・6 Pages: 1183-1190
  • NAID: 10020172629
  • Peer Reviewed
• [Journal Article] Appropriate expression of imprinted genes on mouse chromosome 12 extends development of bi-maternal embryos to term (2007)
  • Author(s): Kawahara M, Kono T, 他2名
  • Journal Title: FEBS Letters 581 Pages: 5178-5184
  • Peer Reviewed
• [Journal Article] Regulated expressions of two sets of paternally imprinted genes is necessary for mouse parthenogenetic development to term (2006)
  • Author(s): Wu, Q., Kono, T. 他6名
  • Journal Title: Reproduction 131 Pages: 481-488
  • Peer Reviewed
• [Journal Article] Oocyte growth-dependent progression of maternal imprinting in mice (2006)
  • Author(s): Hiura, H., Kono, T. 他3名
  • Journal Title: Genes to Cells 11 Pages: 353-361
  • Peer Reviewed
• [Journal Article] Genomic imprinting is a barrier to parthenogenesis in mammals. Cytogenet (2006)
  • Author(s): Kono, T
  • Journal Title: Genome Research 113 Pages: 31-35
  • Peer Reviewed
• [Journal Article] Disruption of parental-specific expression of imprinted genes in uniparental fetuses (2006)
  • Author(s): Ogawa, H., Kono, T. 他3名
  • Journal Title: FEBS Letters 580 Pages: 5377-5384
  • Peer Reviewed
• [Journal Article] The human HYMAI/PLAGL1 differentially methylated region acts as an imprint control region in mice (2006)
  • Author(s): Arima, T., Kono, T. 他6名
  • Journal Title: Genomics 88 Pages: 650-658
  • Peer Reviewed
• [Journal Article] Complementary roles of genes regulated by two paternally methylated imprinted regions on chromosomes 7 and 12 in mouseplacentation (2006)
  • Author(s): Kawahara, M., Kono, T. 他3名
  • Journal Title: Human Molecular Genetics 15 Pages: 2869-2879
  • Peer Reviewed
• [Journal Article] Regulated expressions of two sets of paternally imprinted genes are necessary for mouse parthenogenetic development to term. (2006)
  • Author(s): Wu Q
  • Journal Title: Reproduction 131 Pages: 481-488
• [Journal Article] Oocyte growth-dependent progression of maternal imprinting in mice. (2006)
  • Author(s): Hiura H
  • Journal Title: Genes to Cells 11 Pages: 353-361
• [Journal Article] Genomic imprinting is a barrier to parthenogenesis in mammals. (2006)
  • Author(s): Kono T
  • Journal Title: Cytogenetic and Genome Research 131 Pages: 31-35
• [Journal Article] Disruption of parental-specific expression of imprinted genes in uniparental fetuses. (2006)
  • Author(s): Ogawa H
  • Journal Title: FEBS Letters 580 Pages: 5377-5384
• [Journal Article] The human HYMAI/PLAGL1 differentially methylated region acts as an imprint control region in mice. (2006)
  • Author(s): Arima T
  • Journal Title: Genomics 85 Pages: 650-658
• [Journal Article] Complementary roles of genes regulated by two paternally methylated imprinted regions on chromosomes 7 and 12 in mouse placentation. (2006)
  • Author(s): Kawahara M
  • Journal Title: Human Molecular Genetics 15 Pages: 2869-2879
• [Presentation] Genetic modification for bi-maternal embryo development (2009)
  • Author(s): 河野友宏
  • Organizer: the International Embryo Transfer Society
  • Place of Presentation: 米国
  • Year and Date: 2009-01-05
• [Presentation] Genetic modification for bi-matemal embryo development (2009)
  • Author(s): Kono, Tomohiro.
  • Organizer: Annual Conference of the International Embryo Transfer Society
  • Place of Presentation: SanDiego, USA
  • Year and Date: 2009-01-05
• [Presentation] 単為発生胚を用いた新規インプリント遺伝子の探索 : マウス1番染色体のZdbf2遺伝子は父由来アレル特異的に発現するインプリント遺伝子である (2008)
  • Author(s): 小林久人、外丸祐介、河野友宏
  • Organizer: 日本分子生物学会
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-12
• [Presentation] 単為発生胚を用いた新規インプリント遺伝子の探索 : マウス1番染色体のZdbf2遺伝子は父由来アレル特異的に発現するインプリント遺伝子である (2008)
  • Author(s): 小林 久人, 外丸 祐介, 河野 友宏
  • Organizer: 日本分子生物学会大会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2008-12-12
• [Presentation] 非コードインプリント遺伝子Glt2欠損マウスにおける親の特異的な致死性および遺伝子発現異常 (2008)
  • Author(s): 高橋望、河野友宏
  • Organizer: 日本分子生物学会
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-11
• [Presentation] 非翻訳インプリント遺伝子Glt2欠損マウスにおける致死性および器官形成異常 (2008)
  • Author(s): 岡本晶、河野友宏
  • Organizer: 日本分子生物学会
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-11
• [Presentation] 非コードインプリント遺伝子Glt2欠損マウスにおける親の特異的な致死性および遺伝子発現異常 (2008)
  • Author(s): 高橋 望, 河野 友宏
  • Organizer: 日本分子生物学会大会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2008-12-11
• [Presentation] 非翻訳インプリント遺伝子Glt2欠損マウスにおける致死性および器官形成異常 (2008)
  • Author(s): 岡本晶, 河野 友宏
  • Organizer: 日本分子生物学会大会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2008-12-11
• [Presentation] 単為発生胚を用いた新規インプリント遺伝子の探索 : マウス1番染色体のZdbf2遺伝子は父由来アレル特異的に発現するインプリント遺伝子である (2008)
  • Author(s): 小林久人、外丸祐介、河野友宏
  • Organizer: 日本繁殖生物学会
  • Place of Presentation: 福岡
  • Year and Date: 2008-09-18
• [Presentation] マイクロアレイ方を用いた単一マウスクローン胚盤胞の網羅的遺伝子発現解析 (2008)
  • Author(s): 曹峰、外丸祐介、河野友宏
  • Organizer: 日本繁殖生物学会
  • Place of Presentation: 福岡
  • Year and Date: 2008-09-18
• [Presentation] Non-coding RNAインプリント遺伝子Glt2欠損マウスにおけるRtl1遺伝子の発現解析 (2008)
  • Author(s): 小林亮太、河野友宏
  • Organizer: 日本繁殖生物学会
  • Place of Presentation: 福岡
  • Year and Date: 2008-09-18
• [Presentation] 単為発生胚を用いた新規インプリント遺伝子の探索 : マウス1番染色体のZdbf2遺伝子は父由来アレル特異的に発現するインプリント遺伝子である (2008)
  • Author(s): 小林 久人, 外丸 祐介, 河野 友宏
  • Organizer: 日本繁殖生物学会大会
  • Place of Presentation: 九州大学
  • Year and Date: 2008-09-18
• [Presentation] マイクロアレイ法を用いた単一マウスクローン胚盤胞の網羅的遺伝子発現解析 (2008)
  • Author(s): 曹 峰, 外丸 祐介, 河野 友宏
  • Organizer: 日本繁殖生物学会大会
  • Place of Presentation: 九州大学
  • Year and Date: 2008-09-18
• [Presentation] Non-coding RNA インプリント遺伝子Glt2欠損マウスにおけるRtll遺伝子の発現解析 (2008)
  • Author(s): 小林 亮太, 河野 友宏
  • Organizer: 日本繁殖生物学会大会
  • Place of Presentation: 九州大学
  • Year and Date: 2008-09-18
• [Presentation] Glt2遺伝子欠損マウスにおける器官形成異常 (2008)
  • Author(s): 岡本晶、河野友宏
  • Organizer: 日本哺乳動物卵子学会
  • Place of Presentation: 名古屋
  • Year and Date: 2008-05-27
• [Presentation] Generation of viable bimaternal mice lacking a paternal genome (2008)
  • Author(s): 河野友宏
  • Organizer: World Congress on Reproductive Biology
  • Place of Presentation: 米国
  • Year and Date: 2008-05-24
• [Presentation] Deletion of the maternally expressed imprinted gene, Glt2, resulted in parental-specific lethality in mice (2008)
  • Author(s): 高橋望、河野友宏
  • Organizer: World Congress on Reproductive Biology
  • Place of Presentation: 米国
  • Year and Date: 2008-05-24
• [Presentation] Generation of viable bimatemal mice lacking a paternal genome (2008)
  • Author(s): Kono, Tomohiro.
  • Organizer: World Congress on Reproductive Biology
  • Place of Presentation: Hawaii, USA
  • Year and Date: 2008-05-24
• [Presentation] Deletion of the maternally expressed imprinted gene, Glt2, resulted in parental-specific lethality in mice (2008)
  • Author(s): Takahashi, Nozomi., Kono, Tomohiro
  • Organizer: World Congress on Reproductive Biology
  • Place of Presentation: Hawaii, USA
  • Year and Date: 2008-05-24
• [Presentation] Glt2遺伝子欠損マウスにおける器官形成異常 (2008)
  • Author(s): 岡本 晶, 河野 友宏
  • Organizer: 日本哺乳動物卵子学会大会
  • Place of Presentation: 名古屋国際会議場
  • Year and Date: 2008-05-17
• [Presentation] 雄の遺伝的関与を欠いたマウスの効率的な生産 (2007)
  • Author(s): 川原学、河野友宏
  • Organizer: 日本繁殖生物学会
  • Place of Presentation: 東京
  • Year and Date: 2007-10-20
• [Presentation] 二母性マウス表現型の特徴 (2007)
  • Author(s): 河野友宏
  • Organizer: 日本繁殖生物学会
  • Place of Presentation: 東京
  • Year and Date: 2007-10-20
• [Presentation] Regulation of mammalian development by genomic imprinting (2007)
  • Author(s): 河野友宏
  • Organizer: International Young Scientist Forum on Mammalian Reproductive Biotechnology
  • Place of Presentation: 中国
  • Year and Date: 2007-08-28
• [Presentation] マイクロアレイ解析を用いたマウス新規インプリント遺伝子の探査 (2007)
  • Author(s): 山田 かおり
  • Organizer: 日本哺乳動物卵子学会
  • Place of Presentation: 甲府
  • Year and Date: 2007-05-27
• [Presentation] 父母メチル化インプリントの責務 (2007)
  • Author(s): 河野友宏
  • Organizer: 日本哺乳動物卵子学会
  • Place of Presentation: 山梨
  • Year and Date: 2007-05-17
• [Presentation] マイクロアレイ解析を用いたマウス新規インプリント遺伝子の探査 (2007)
  • Author(s): 山田かおり、外丸祐介、河野友宏
  • Organizer: 日本哺乳動物卵子学会
  • Place of Presentation: 山梨
  • Year and Date: 2007-05-17
• [Presentation] Establishment of trophoblastic stem like cell from mouse parthenogenetic embryos (2007)
  • Author(s): 小川英彦、河野友宏
  • Organizer: International Embryo Transfer Sciety
  • Place of Presentation: 京都
  • Year and Date: 2007-01-06
• [Presentation] Developmental competence of mouse ooytes after in vitro groeth nuclear transfer, and in vitro fertilization (2007)
  • Author(s): 尾畑やよい、河野友宏
  • Organizer: International Embryo Transfer Sciety
  • Place of Presentation: 京都
  • Year and Date: 2007-01-06
• [Presentation] 二母性マウス作出系の確立 (2007)
  • Author(s): 川原 学
  • Organizer: 日本畜産学会
  • Place of Presentation: 相模原
• [Presentation] Complementary roles of Igf2and Dlk1 for descendants (2006)
  • Author(s): 河野友宏
  • Organizer: International Genomic Imprinting Workshop
  • Year and Date: 2006-11-29
• [Presentation] 雄核発生胚および雌核発生胚由来栄養膜幹細胞におけるCdx2 遺伝子の発現 (2006)
  • Author(s): 小川英彦、河野友宏
  • Organizer: 日本繁殖生物学会
  • Place of Presentation: 名古屋
  • Year and Date: 2006-09-07
• [Presentation] B6-Y 性転換マウス由来卵母細胞の発生特性 (2006)
  • Author(s): 尾畑やよい、河野友宏
  • Organizer: 日本繁殖生物学会
  • Place of Presentation: 名古屋
  • Year and Date: 2006-09-07
• [Presentation] Effect of lgf2 and Dlk1 genes on hematopoiesis in mouse (2006)
  • Author(s): 呉瓊、河野友宏
  • Organizer: 日本繁殖生物学会
  • Place of Presentation: 名古屋
  • Year and Date: 2006-09-07
• [Presentation] マウス胎盤形成における7 番染色体および12 番染色体上の父性インプリント遺伝子の役割 (2006)
  • Author(s): 川原学、河野友宏
  • Organizer: 日本繁殖生物学会
  • Place of Presentation: 名古屋
  • Year and Date: 2006-09-07
• [Presentation] マウスESクローン胚盤胞期における特異的発現遺伝子の網羅的探査 (2006)
  • Author(s): 神長祐子、河野友宏
  • Organizer: 日本繁殖生物学会
  • Place of Presentation: 名古屋
  • Year and Date: 2006-09-07
• [Presentation] マウス父性インプリント遺伝子の精子形成過程におけるDNAメチル化修飾獲得時期の特定 (2006)
  • Author(s): 白井求、河野友宏
  • Organizer: 日本繁殖生物学会
  • Place of Presentation: 名古屋
  • Year and Date: 2006-09-07
• [Presentation] マイクロアレイ解析を用いたマウス新規インプリント遺伝子の探査 (2006)
  • Author(s): 山田かおり、河野友宏
  • Organizer: 日本繁殖生物学会
  • Place of Presentation: 名古屋
  • Year and Date: 2006-09-07
• [Presentation] 非コードインプリント遺伝子Gt12欠損マウスの発生解析 (2006)
  • Author(s): 高橋望、河野友宏
  • Organizer: 日本繁殖生物学会
  • Place of Presentation: 名古屋
  • Year and Date: 2006-09-07
• [Presentation] Maturation of prcine oocytes reconstructed with a nucleus from the growing stage oocytes (2006)
  • Author(s): 松崎隆、河野友宏
  • Organizer: Society for the Study of Reproduction
  • Place of Presentation: 米国
  • Year and Date: 2006-07-29
• [Presentation] Genes controlled by two paternally imprinted regions differentiallycontribute to mouse placentation (2006)
  • Author(s): 川原学、河野友宏
  • Organizer: Society for Reproduction and Fertility Conference
  • Place of Presentation: 英国
  • Year and Date: 2006-07-03
• [Presentation] マウス卵母細胞におけるインプリント遺伝子のヒストン修飾解析 (2006)
  • Author(s): 樋浦仁、河野友宏
  • Organizer: 日本分子生物学会
  • Place of Presentation: 名古屋
• [Patent(Industrial Property Rights)] 核移植卵、単為発生胚および単為発生哺乳動物の作出法 (2009)
  • Inventor(s): 河野友宏、尾畑やよい
  • Industrial Property Rights Holder: 東京農業大学
  • Acquisition Date: 2009-03-21
• [Patent(Industrial Property Rights)] Method of Constructing Nuclear-Transplanted Egg, Parthenogenetic Embryo and Parthenogenetic Mammal (2009)
  • Inventor(s): 河野友宏、尾畑やよい
  • Industrial Property Rights Holder: 東京農業大学
  • Acquisition Date: 2009-02-25
  • Overseas
• [Patent(Industrial Property Rights)] 特許権 (2009)
  • Inventor(s): 河野友宏, 尾畑やよい
  • Industrial Property Rights Holder: 東京農業大学
  • Acquisition Date: 2009-03-13
• [Patent(Industrial Property Rights)] 特許権 (2009)
  • Inventor(s): 河野友宏, 尾畑やよい
  • Industrial Property Rights Holder: 東京農業大学
  • Acquisition Date: 2009-02-25
  • Overseas
• [Patent(Industrial Property Rights)] 核移植卵、単為発生胚および単為発生哺乳動物の作出方法 (2007)
  • Inventor(s): 河野友宏, 川原学
  • Industrial Property Rights Holder: 東京農業大学
  • Industrial Property Number: 2007-171707
  • Filing Date: 2007-06-29