Regulatory Mechanism of Sperm Function in Female Reproductive Tract
Project/Area Number |
18208029
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Research Category |
Grant-in-Aid for Scientific Research (A)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Applied molecular and cellular biology
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Research Institution | University of Tsukuba |
Principal Investigator |
BABA Tadashi University of Tsukuba, 大学院・生命環境科学研究科, 教授 (40165056)
|
Co-Investigator(Kenkyū-buntansha) |
NAKANISHI Tomoko 筑波大学, 大学院・生命環境科学研究科, 助教 (10344863)
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Project Period (FY) |
2006 – 2008
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Project Status |
Completed (Fiscal Year 2008)
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Budget Amount *help |
¥37,050,000 (Direct Cost: ¥28,500,000、Indirect Cost: ¥8,550,000)
Fiscal Year 2008: ¥10,140,000 (Direct Cost: ¥7,800,000、Indirect Cost: ¥2,340,000)
Fiscal Year 2007: ¥13,910,000 (Direct Cost: ¥10,700,000、Indirect Cost: ¥3,210,000)
Fiscal Year 2006: ¥13,000,000 (Direct Cost: ¥10,000,000、Indirect Cost: ¥3,000,000)
|
Keywords | 受精 / 子宮 / 輸卵管 / 精子機能 / マウス |
Research Abstract |
精子が雌性生殖器内で子宮から輸卵管へ移動し、卵管膨大部で卵子と融合するまでの過程で起こるさまざまな仕組みを明らかにするために研究を行った。精子セリンプロテアーゼPRSS21の欠損マウス精子を調べ、子宮や卵管分泌液中には精子の受精能を賦与する因子が存在することを発見した。また、PRSS21が透明帯上での精子アクロソーム反応で重要な役割を果たしていることも明確にした。さらに、精子ヒアルロニダーゼHYAL5 SPAM1の欠損マウス精子を解析し、受精にはヒアルロニダーゼが必須ではないことを証明し、卵子卵丘細胞複合体に精子排除機構があることを提唱した。
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Report
(5 results)
Research Products
(33 results)
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[Journal Article] Functional roles of mouse sperm hyaluronidases, HYAL5 and SPAM1, in fertilization2009
Author(s)
Kimura, M., Kim, E., Kang, W., Yamashita, M., Saigo, M., Yamazaki, T., Nakanishi, T., Kashiwabara, S., Baba, T.
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Journal Title
Biol.Reprod. 81
Pages: 939-947
Related Report
Peer Reviewed
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[Journal Article] Reduced fertility of mouse epididymal sperm lacking Prss21/Tesp5 is rescued by sperm exposure to uterine microenvironment2008
Author(s)
Yamashita, M., Honda, A., Ogura, A., Kashiwabara, S., Fukami, K., Baba, T.
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Journal Title
Genes Cells 13
Pages: 1001-1013
Related Report
Peer Reviewed
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[Journal Article] Sperm penetration through cumulus mass and zona pellucida2008
Author(s)
Kim, E., Yamashita, M., Kimura, M., Honda, A., Kashiwabara, S., Baba, T.
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Journal Title
Int.J.Dev.Biol. 52
Pages: 677-682
Related Report
Peer Reviewed
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[Journal Article] Reduced fertility of mouse epididymal sperm lacking Prss21/Tesp5 is rescued by sperm exposure to uterine microenvironment2008
Author(s)
Yamashita, M., Honda, A., Ogura, A., Kashiwabara, S., Fukami, K., and Baba, T.
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Journal Title
Genes Cells 13
Pages: 1001-1013
Related Report
Peer Reviewed
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[Journal Article] Sperm penetration through cumulus mass and zona pellucida2008
Author(s)
Kim, E., Yamashita, M., Kimura, M., Honda, A., Kashiwabara, S., and Baba, T.
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Journal Title
Int. J. Dev. Biol 52
Pages: 677-682
Related Report
Peer Reviewed
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[Journal Article] Sperm penetration through cumulus mass and zona pellucida.2008
Author(s)
Kim, E., Yamashita, M., Kimura, M., Honda, A.,Kashiwabara, S., and Baba, T.
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Journal Title
Int. J. Dev. Biol. 52
Pages: 667-682
Related Report
Peer Reviewed
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[Journal Article] Reduced fertility of mouse sperm lacking Prss21/Tesp5 is rescued by sper mexposure to uterine microenvironment.2008
Author(s)
Yamashita, M., Honda, A., Ogura, A., Kashiwabara, S., Fukami, K., and Baba, T.
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Journal Title
Genes Calls 13
Pages: 1001-1013
Related Report
Peer Reviewed
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[Journal Article] Birth of normal offspring from mouse eggs activated by a phospholipase Cζ protein lacking three EF-hand domains.2008
Author(s)
Nakanishi, T., Ishibashi, N., Kubota, H., Inoue, K., Ogonuki, N., Ogura, A.. Kashiwabara. S.. And Baba. T.
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Journal Title
J. Reprod, Dev. 54
Pages: 244-249
NAID
Related Report
Peer Reviewed
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[Journal Article] Sperm penetration through cumulus mass and zona pellucida.2008
Author(s)
Kim, E., Yamashita, M., Kimura, M., HOnda, A., Kashiwabara, S., and Baba, T.
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Journal Title
Related Report
Peer Reviewed
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[Journal Article] Mouse sperm lacking ADAM1b/ADAM2 fertilin can fuse with the egg plasma membrane and effect fertilization2006
Author(s)
Kim, E., Yamashita, M., Nakanishi, T., Park, K. -E., Kimura, M., Kashiwabara, S., Baba, T.
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Journal Title
J.Biol.Chem. 281
Pages: 5634-5639
Related Report
Peer Reviewed
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[Journal Article] Mouse sperm lacking ADAM1b/ADAM2 fertilin can fuse with the egg plasma membrane and effect fertilization2006
Author(s)
Kim, E., Yamashita, M., Nakanishi, T., Park, K. -E., Kimura, M., Kashiwabara, S., and Baba, T.
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Journal Title
J. Biol. Chem 281
Pages: 5634-5639
Related Report
Peer Reviewed
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[Presentation] Mouse sperm lacking serine protease Prss21/Tesp5 barely undergo the zona pellucida-induced acrosome reaction, but do fertilize the egg2008
Author(s)
Yamashita, M., Honda, A., Ogura, A., Kashiwabara, SI., Fukami, K., Baba, T.
Organizer
World Congress on Reproductive Biology
Place of Presentation
Hawaii (USA)
Related Report
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