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Analysis of generation and control mechanism of CD8+ T cells by chemokines

Research Project

Project/Area Number 18209016
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Immunology
Research InstitutionThe University of Tokyo

Principal Investigator

MATSUSHIMA Kouji  The University of Tokyo, 大学院・医学系研究科, 教授 (50222427)

Co-Investigator(Kenkyū-buntansha) 橋本 真一  東京大学, 大学院・医学系研究科, 助教 (00313099)
垣見 和宏  東京大学, 医学部附属病院, 寄付講座教員(客員准教授) (80273358)
倉知 慎  東京大学, 大学院・医学系研究科, 助教 (00396722)
島岡 猛士  東京大学, 大学院・医学系研究科, 特任助教 (90422279)
Co-Investigator(Renkei-kenkyūsha) HASHIMOTO Shinichi  東京大学, 大学院・医学系研究科, 助教 (00313099)
KAKIMI Kazuhiro  東京大学, 医学部・附属病院, 客員准教授 (80273358)
MAKOTO KURACHI  東京大学, 大学院・医学系研究科, 助教 (00396722)
SHIMAOKA Takeshi  東京大学, 大学院・医学系研究科, 特任助教 (90422279)
Research Collaborator UEHA Satoshi  東京大学, 大学院・医学系研究科, 助教 (00447385)
Project Period (FY) 2006 – 2008
Project Status Completed (Fiscal Year 2008)
Budget Amount *help
¥48,750,000 (Direct Cost: ¥37,500,000、Indirect Cost: ¥11,250,000)
Fiscal Year 2008: ¥9,490,000 (Direct Cost: ¥7,300,000、Indirect Cost: ¥2,190,000)
Fiscal Year 2007: ¥18,980,000 (Direct Cost: ¥14,600,000、Indirect Cost: ¥4,380,000)
Fiscal Year 2006: ¥20,280,000 (Direct Cost: ¥15,600,000、Indirect Cost: ¥4,680,000)
Keywordsケモカイン / CTL / 免疫記憶 / 免疫応答の場 / 次世代DNA シークエンサー / トランスクリプトーム解析 / Myeloid derived suppressor cells / マクロファージ / 誘導 / 免疫記憶の維持 / 次世代DNAシークエンサー / 場 / 好中球 / 維持
Research Abstract

T 細胞受容体トランスジェニックマウス細胞を用いて反復感染応答におけるCTL 細胞群の消長を検討した。ある抗原特異的CTL 集団に着目した場合、メモリー期のみならず、エフェクター期から、抗原経験回数が異なるCTL 群が、生体内で異なる分布を示していて、個体レベルでは抗原特異的CTL 集団の維持に働いていることを明らかにした。また、Naive CD8 陽性T 細胞、一次メモリー、二次メモリーCTL に発現している転写産物(タグ)を解析した。

Report

(4 results)
  • 2008 Annual Research Report   Final Research Report ( PDF )
  • 2007 Annual Research Report
  • 2006 Annual Research Report
  • Research Products

    (26 results)

All 2009 2008 2007 2006 Other

All Journal Article (17 results) (of which Peer Reviewed: 11 results) Presentation (6 results) Book (2 results) Remarks (1 results)

  • [Journal Article] High-resolution analysis of the 5'-end transcriptome using a next generation DNA sequencer.2009

    • Author(s)
      Hashimoto S, Qu W, Ahsan B, Ogoshi K,Sasaki A, Nakatani Y, Lee Y, Ogawa M,Ametani A, Suzuki Y, Sugano S, Lee CC,Nutter RC, Morishita S,Matsushima K
    • Journal Title

      PLoS ONE 4(1)

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Chromatin-associated periodicity in genetic variation downstream of transcriptional start sites.2009

    • Author(s)
      Sasaki S, Mello CC, Shimada A, Nakatani Y,Hashimoto S, Ogawa M, Matsushima K, Gu SG,Kasahara M, Ahsan B, Sasaki A, Saito T,Suzuki Y, Sugano S, Kohara Y, Takeda H, Fire A, Morishita S
    • Journal Title

      Science 323(5912)

      Pages: 401-4

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] High-resolution analysis of the 5'-end transcriptome using a next generation DNA sequencer.2009

    • Author(s)
      橋本 真一
    • Journal Title

      PLoS ONE 4

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] SAGE application in hematological research.2008

    • Author(s)
      Hashimoto S, Matsushima K
    • Journal Title

      Curr Pharm Biotechnol 9(5)

      Pages: 383-391

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Shawkat S, Karima R, Tojo T, Tadakuma H, Saitoh S, Akashi-Takamura S, Miyake K, Funatsu T, Matsushima K2008

    • Author(s)
      Visualization of the molecular dynamics of lipopolysaccharide on the plasma membrane of murine macrophages by total internal reflection fluorescence microscopy
    • Journal Title

      J Biol Chem 283(34)

      Pages: 22962-71

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Chemokine-mediated rapid turnover of myeloid-derived suppressor cells in tumor-bearing mice2008

    • Author(s)
      Sawanobori Y, Ueha S, Kurachi M, Shimaoka T, Talmadge JE, Abe J, Shono Y, Kitabatake M, Kakimi K, Mukaida N, Matsushima K
    • Journal Title

      Blood 111(12)

      Pages: 5457-66

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Chemokine-mediated rapid turnover of myeloid-derived suppressor cells in tumor-bearing mice2008

    • Author(s)
      Sawanobori Y, et. al.
    • Journal Title

      Blood (掲載確定)(印刷中)

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] CCR7 mediates the migration of Foxp3+ regulatory T cells to the paracortical areas of peripheral lymph nodes through high endothelial venules2007

    • Author(s)
      Ueha S, Yoneyama H, Hontsu S, Kurachi M, Kitabatake M, Abe J, Yoshie O, Shibayama S, Sugiyama T, Matsushima K
    • Journal Title

      J Leukoc Biol 82(5)

      Pages: 1230-1230

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] The medaka draft genome and insights into vertebrate genome evolution2007

    • Author(s)
      Kasahara M, Naruse K, Sasaki S, Nakatani Y, Qu W, Ahsan B, Yamada T, Nagayasu Y, Doi K, Kasai Y, Jindo T, Kobayashi D, Shimada A, Toyoda A, Kuroki Y, Fujiyama A, Sasaki T, Shimizu A, Asakawa S, Shimizu N,Hashimoto S, Yang J, Lee Y, Matsushima K,Sugano S, Sakaizumi M, Narita T, Ohishi K,Haga S, Ohta F, Nomoto H, Nogata K,Morishita T, Endo T, Shin-I T, Takeda H,Morishita S, Kohara Y
    • Journal Title

      Nature 447(7145)

      Pages: 714-9

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] 8)Maintenance of memory CD8+ T cell diversity and proliferative potential by a primary response upon re-challenge2007

    • Author(s)
      Kurachi M, Kakimi K, Ueha S, Matsushima K
    • Journal Title

      Int Immunol 19(1)

      Pages: 105-15

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] CCR7 mediates the migration of Foxp3+ regulatory T cells to the paracortical areas of peripheral lymph nodes through high endothelialvenules2007

    • Author(s)
      Ueha S, et. al.
    • Journal Title

      J Leukoc Biol 82

      Pages: 1230-1238

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Intervention of MAdCAM-1 or Fractalkine alleviates graft-versus-host reaction associated intestinal injury while preserving graft-versus-tumor effects.2007

    • Author(s)
      Ueha S, et al.
    • Journal Title

      J Leukoc Biol. 81(1)

      Pages: 176-185

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Maintenance of memory CD8+ T cell diversity and proliferative potential by a primary response upon re-challenge.2007

    • Author(s)
      Kurachi M, et al.
    • Journal Title

      Int Immunol. 19(1)

      Pages: 105-115

    • NAID

      40015794096

    • Related Report
      2006 Annual Research Report
  • [Journal Article] SARS-CoV spike protein-expressing recombinant vaccinia virus efficiently induces neutralizing antibodies in rabbits pre-immunized with vaccinia virus.2007

    • Author(s)
      Kitabatake M, et al.
    • Journal Title

      Vaccine. 25(4)

      Pages: 630-637

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Combined therapy of transcatheter hepatic arterial embolization with intratumoral dendritic cell infusion for hepatocellular carcinoma : clinical safety.2007

    • Author(s)
      Nakamoto Y, et al.
    • Journal Title

      Clin Exp Immunol. 147(2)

      Pages: 296-305

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Secondary lymphoid tissue chemokine (SLC/CCL21)/CCR7 signaling regulates fibrocytes in renal fibrosis.2006

    • Author(s)
      Sakai N, et al.
    • Journal Title

      Proc Natl Acad Sci U S A. 103(38)

      Pages: 14098-14103

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Reduction in Chronic Allograft Nephropathy by Inhibition of p38 Mitogen-Activated Protein Kinase.2006

    • Author(s)
      Wada T, et al.
    • Journal Title

      Am J Nephrol. 26(4)

      Pages: 319-325

    • Related Report
      2006 Annual Research Report
  • [Presentation] Primary Memory CTL preferentially accumulate in lymph nodes2009

    • Author(s)
      Kurachi M, Kakimi K, Ueha S, Suenaga F, Abe J, Matsushima K
    • Organizer
      Keystone Symposia. Immunologic Memory and Host Defense
    • Place of Presentation
      Keystone, Colorado
    • Year and Date
      2009-02-10
    • Related Report
      2008 Final Research Report
  • [Presentation] Primary memory CTL preferentially accumulate in secondary lymphoid organs2008

    • Author(s)
      倉知慎、垣見和宏、上羽悟史、末永文子、阿部淳、松島綱治
    • Organizer
      第38 回日本免疫学会総会・学術総会
    • Place of Presentation
      第38 回日本免疫学会総会・学術総会
    • Year and Date
      2008-12-02
    • Related Report
      2008 Final Research Report
  • [Presentation] Chemokine-mediated rapid turnover of myeloid-derived suppressor cells in tumor-bearing mice2008

    • Author(s)
      上羽悟史
    • Organizer
      第38 回日本免疫学会総会・学術集会
    • Place of Presentation
      京都市左京区・国立京都国際会館
    • Year and Date
      2008-12-02
    • Related Report
      2008 Final Research Report
  • [Presentation] Primary memory CTL preferentially accumulate in secondary lymphoid organs2008

    • Author(s)
      倉地 慎, 他
    • Organizer
      第38回日本免疫学会総会・学術総会
    • Place of Presentation
      京都(国立京都国際会館)
    • Year and Date
      2008-12-02
    • Related Report
      2008 Annual Research Report
  • [Presentation] Chemokine-mediated rapid turnover of myeloid-derived suppressor cells in tumor-bearing mice2008

    • Author(s)
      上羽悟史
    • Organizer
      第67 回日本癌学会学術総会
    • Place of Presentation
      名古屋市熱田区・名古屋国際会議場
    • Year and Date
      2008-10-28
    • Related Report
      2008 Final Research Report
  • [Presentation] CTL一次応答による抗原特異的CTL応答の維持2007

    • Author(s)
      倉知 慎, 他
    • Organizer
      第37回目本免疫学会総会学術集会
    • Place of Presentation
      大阪
    • Year and Date
      2007-11-22
    • Related Report
      2007 Annual Research Report
  • [Book] Minophargen Medical Review2009

    • Author(s)
      上羽悟史、松島綱治
    • Publisher
      ケモカインによる制御性T細胞と骨髄系抑制性細胞の担癌宿主内動態制御(第54 巻1 号)
    • Related Report
      2008 Final Research Report
  • [Book] エフェクターCD8+T 細胞とメモリーCD8+T 細胞のバランス決定要因(月刊 臨床免疫・アレルギー科)2008

    • Author(s)
      倉知慎、垣見和宏、上羽悟史、松島綱治
    • Publisher
      科学評論社(Vol.49(5))
    • Related Report
      2008 Final Research Report
  • [Remarks]

    • URL

      http://www.prevent.m.u-tokyo.ac.jp/

    • Related Report
      2008 Annual Research Report

URL: 

Published: 2006-04-01   Modified: 2016-04-21  

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