Analysis of reorganization of neuronal circuitry with newly generated neurons in the olfactory bulb by selective ablation of preexisting neurons

• Project/Area Number: 18300103
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Neuroscience in general
• Research Institution: The University of Tokyo
• Principal Investigator: YAMAGUCHI Masahiro The University of Tokyo, 大学院・医学系研究科, 講師 (60313102)
• Project Period (FY): 2006 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥16,850,000 (Direct Cost: ¥14,300,000、Indirect Cost: ¥2,550,000); Fiscal Year 2008: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2007: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000); Fiscal Year 2006: ¥5,800,000 (Direct Cost: ¥5,800,000)
• Keywords: 神経科学 / 再生医学 / 嗅球 / 神経回路

Research Abstract

成体嗅球では新しい神経細胞が神経回路に組み込まれているが、これが既存の神経細胞にどの様に影響されるかは不明である。遺伝子操作マウスを用いて嗅球の既存の神経細胞のサブタイプ(mGluR2陽性細胞)を選択的に除去したところ、新生mGluR2陽性顆粒細胞が優先的に組み込まれ、2ヶ月後にはmGluR2 陽性顆粒細胞がほぼ元通りに回復した。新生神経細胞は、不足した既存の神経細胞サブタイプを補って、神経回路を修復する能力を持つことが明らかとなった。

Research Products

• [Journal Article] Perisomatic-targeting granule cells in the mouse olfactory bulb (2009)
  • Author(s): Naritsuka H, Sakai K, Hashikawa T, Mori K, Yamaguchi M
  • Journal Title: Journal of Comparative Neurology 515 Pages: 409-426
  • Peer Reviewed
• [Journal Article] Perisomatic-targeting granule cells in the mouse olfactory bulb. (2009)
  • Author(s): Naritsuka H, Sakai K, Hashikawa T, Mori K, Yamaguchi M.
  • Journal Title: Journal of Comparative Neurology (印刷中 掲載確定)
  • Peer Reviewed
• [Journal Article] Roles of continuous neurogenesis in the structural and functional integrity of the adult forebrain (2008)
  • Author(s): Imayoshi I, Sakamoto M, Ohtsuka T, Miyakawa T, Yamaguchi M, Mori K, Ikeda T, Itohara S, Kageyama R
  • Journal Title: Nature Neuroscience 11 Pages: 1153-1161
  • Peer Reviewed
• [Journal Article] Time course of morphine's effects on adult hippocampal subgranular zone reveals preferential inhibition of cells in S phase of the cell cycle and a subpopulation of immature neurons. (2008)
  • Author(s): Arguello AA, Harburg GC, Schonborn JR, Mandyam CD, Yamaguchi M, Eisch AJ.
  • Journal Title: Neuroscience 157 Pages: 70-79
  • Peer Reviewed
• [Journal Article] Roles of continuous neurogenesis in the structural and functional integrity of the adult forebrain. (2008)
  • Author(s): Imayoshi I, Sakamoto M, Ohtsuka T, Miyakawa T, Yamaguchi M, Mori K, Ikeda T, Itohara S, Kageyama R.
  • Journal Title: Nature Neuroscience 11 Pages: 1153-1161
  • Peer Reviewed
• [Journal Article] Neurogenesis and alterations of neural stem cells in mouse models of cerebral amyloidosis. (2008)
  • Author(s): Ermini FV, Grathwohl S, Radde R, Yamaguchi M, Staufenbiel M, Palmer TD, Jucker M.
  • Journal Title: American Journal of Pathology 172 Pages: 1520-1528
  • Peer Reviewed
• [Journal Article] Dynamic expression of TrkB receptor protein on proliferating and maturing cells in the adult mouse dentate gyrus (2008)
  • Author(s): Donovan, M.H., Yamaguchi, M., Eisc, A.J.
  • Journal Title: Hippocampus (掲載確定)
  • Peer Reviewed
• [Journal Article] Local origin and activity-dependent generation of nestin-exprpressing protoplasmic astrocytes in CA1 (2007)
  • Author(s): Kronenberg, G, Wang, L.P., Geraerts, M., Babu, H., Synowitz, M., Vicens, P, Lutsch, G, Glass, R., Yamaguchi, M., Baekelandt, V. Debyse, r Z., Kettenmann, H., Keme, G
  • Journal Title: Brain Structure and Function 212 Pages: 19-35
  • Peer Reviewed
• [Journal Article] Genetic modification does not affect the stemess of neural stem cells nestin promoter-GFP transgenic mice (2007)
  • Author(s): Park, J.H., Ahn, J.I, Kim, S.Y., Park, K.S., Lee, Y.D., Yamaguchi M., Chun, H.J.
  • Journal Title: Neuroscience Letters 421 Pages: 185-190
  • Peer Reviewed
• [Journal Article] Enhanced cell-to-cell contacts between activated microglia and pyramidal cell dendrites following kainic acid-induced neurotoxicity in the hippocampus. (2007)
  • Author(s): Hasegawa, S., Yamaguchi, M., Nagao, H., Mishina, M., Mori,K.
  • Journal Title: Journal of Neuroimmunology (In press)
• [Journal Article] Multipotent cells from mammalian iris pigment epithelium. (2007)
  • Author(s): Asami, M., Sun, G., Yamaguchi, M., Kosaka,M.
  • Journal Title: Developmental Biology 304 Pages: 433-436
• [Journal Article] Combination of growth factors enhances remyelination in a cuprizone-induced demyelination mouse model. (2006)
  • Author(s): Kumar, S., Biancotti, J.C., Yamaguchi, M., de Vellis, J.
  • Journal Title: Neurochemical Reserch 32 Pages: 783-797
• [Journal Article] Sphere formation of ocular epithelial cells in the ciliary body is a reprogramming system for neural differentiation. (2006)
  • Author(s): Kohno, R., Ikeda, Y., Yonemitsu, Y., Hisatomi, T., Yamaguchi, M., Miyazaki, M., Takeshita, H., Ishibashi, T., Sueishi,K.
  • Journal Title: Brain Research 1093 Pages: 54-70
• [Journal Article] Increase in bFGF-responsive neural progenitor population following contusion injury of the adult rodent spinal cord. (2006)
  • Author(s): Xu, Y., Kitada, M., Yamaguchi, M., Dezawa, M., Ide,C.
  • Journal Title: Neuroscience Letters 397 Pages: 174-179
• [Journal Article] Maps of odorant molecular features in the mammalian olfactory bulb. (2006)
  • Author(s): Mori, K., Takahashi, T.K., Igarashi, K.M., Yamaguchi,M.
  • Journal Title: Physiological Reviews 86 Pages: 409-433
• [Presentation] Time windows for the elimination of adult-born olfactory bulb neurons (2008)
  • Author(s): Yamaguchi M.
  • Organizer: 第31回日本分子生物学会大会、第81回日本生化学大会合同大会
  • Place of Presentation: 神戸、神戸ポートアイランド
  • Year and Date: 2008-12-09
• [Presentation] Time windows for the fate decision of adult-born neurons in the mouse olfactory bulb (2008)
  • Author(s): Yamaguchi M.
  • Organizer: 6th International Symposium on Molecular and Neural Mechanisms of Taste and Olfactory Perception
  • Place of Presentation: 福岡、九州大学西新プラザ
  • Year and Date: 2008-12-07
• [Presentation] Compensatory incorporation of newborn granule cells in the adult mouse olfactory bulb (2008)
  • Author(s): Murata K, Nakanishi S, Kobayashi K, Mori K, Yamaguchi M
  • Organizer: Neuroscience 2008
  • Place of Presentation: Washington DC, USA
  • Year and Date: 2008-11-17
• [Presentation] Eating-related time windows for apoptotic elimination of adult-born neurons in the mouse olfactory bulb (2008)
  • Author(s): Yokoyama K, Murata K, Mori K, Yamaguchi M.
  • Organizer: Neuroscience 2008
  • Place of Presentation: Washington DC, USA
  • Year and Date: 2008-11-15
• [Presentation] 成体マウス嗅球における介在神経細胞の入れ替わり (2008)
  • Author(s): 山口正洋
  • Organizer: 第31回日本神経科学大会
  • Place of Presentation: 東京・東京国際フォーラム
  • Year and Date: 2008-07-11
• [Presentation] マウス嗅球新生ニューロンの生死決定は食餌時間におこる (2008)
  • Author(s): 横山健、村田航志、森憲作、山口正洋
  • Organizer: 第31回日本神経科学大会
  • Place of Presentation: 東京、東京国際フォーラム
  • Year and Date: 2008-07-11
• [Presentation] 成体マウス嗅球における介在神経細胞の入れ替わり (2008)
  • Author(s): 山口正洋
  • Organizer: 第31回日本神経科学大会
  • Place of Presentation: 東京、東京国際フォーラム
  • Year and Date: 2008-07-11
• [Presentation] 投射ニューロンの細胞体に限局して抑制性シナプスを形成するマウス嗅球介在ニューロンの解析 (2008)
  • Author(s): 成塚裕美, 境和久, 端川勉, 森憲作, 山口正洋
  • Organizer: 第31回日本神経科学大会
  • Place of Presentation: 東京・東京国際フォーラム
  • Year and Date: 2008-07-10
• [Presentation] 投射ニューロンの細胞体に限局して抑制性シナプスを形成するマウス嗅球介在ニューロンの解析 (2008)
  • Author(s): 成塚裕美、境和久、端川勉、森憲作、山口正洋
  • Organizer: 第31回日本神経科学大会
  • Place of Presentation: 東京、東京国際フォーラム
  • Year and Date: 2008-07-10
• [Presentation] マウス嗅球におけるイムノトキシンによる細胞除去後のmGluR2陽性顆粒細胞サブタイプの優先的組み込み (2008)
  • Author(s): 村田航志, 中西, 重忠, 小林和人, 森憲作, 山口正洋
  • Organizer: 第31回日本神経科学大会
  • Place of Presentation: 東京・東京国際フォーラム
  • Year and Date: 2008-07-09
• [Presentation] マウス嗅球におけるイムノトキシンによる細胞除去後のmGluR2陽性顆粒細胞サブタイプの優先的組み込み (2008)
  • Author(s): 村田航志、中西重忠、小林和人、森憲作、山口正洋
  • Organizer: 第31回日本神経科学大会
  • Place of Presentation: 東京、東京国際フォーラム
  • Year and Date: 2008-07-09
• [Presentation] マウス嗅球顆粒細胞サブタイプ除去後の新生顆粒細胞の補償的組み込み (2007)
  • Author(s): 村田航志, 中西重忠, 小林和人, 森憲作, 山口正洋
  • Organizer: 第30回日本神経科学大会
  • Place of Presentation: 横浜・パシフィコ横浜
  • Year and Date: 2007-09-12
• [Presentation] マウス嗅球顆粒細胞サブタイプ除去後の新生顆粒細胞の補償組み込 (2007)
  • Author(s): 村田 航志、中西 重忠、小林 和人、森 憲作、山口 正洋
  • Organizer: 第30回日本神経科学大会
  • Place of Presentation: 横浜
  • Year and Date: 2007-09-12
• [Presentation] Compensatory regeneration of ablated subtype of granule cells after immunotoxin-mediated cell ablation in the mouse olfactory bulb (2007)
  • Author(s): Murata K, Nakanishi S, Mori K, Yamaguchi M
  • Organizer: 7 th IBRO World congress of neuroscience
  • Place of Presentation: Melbourne, Australia
  • Year and Date: 2007-07-14
• [Presentation] Compensatory regeneration of ablated subtype of granule cells after immunotoxin-mediated cell ablation in the mouse olfactory bulb (2007)
  • Author(s): Murata, K., Nakanishi, S., Kobayashi, K., Mori, K., Yamaguchi, M.
  • Organizer: 7th IBRO World congress of neuroscience
  • Place of Presentation: Melboume,Australia
  • Year and Date: 2007-07-14
• [Presentation] Increased incorporation of ablated subtype of newborn granule cells following immunotoxin-mediated cell ablation in the adult mouse olfactory bulb (2007)
  • Author(s): Murata K, Nakanishi S, Mori K, Yamaguchi M
  • Organizer: Neurogenesis 2007
  • Place of Presentation: okyo, Nihon Mirai-kan
  • Year and Date: 2007-05-16
• [Presentation] Increased incorporation of ablated subtype of newborn granule cells following immunotoxin-mediated cell ablation in the adult mouse olfactory bulb (2007)
  • Author(s): Mumta, K., Nakanishi, S., Kobayashi, K., Mori, K., Yamaguchi M.
  • Organizer: Neurogenesis 2007
  • Place of Presentation: Tokyo,Japan
  • Year and Date: 2007-05-16
• [Presentation] 投射ニューロンの細胞体に抑制性シナプスを形成するマウス嗅球顆粒細胞の解析 (2006)
  • Author(s): 成塚裕美, 境和久, 端川勉, 森憲作, 山口正洋
  • Organizer: 第29回日本神経科学大会
  • Place of Presentation: 京都・国立京都国際会館
  • Year and Date: 2006-07-21