Functional analysis of presynaptic proteins involved in facilitation of synaptic transmission

• Project/Area Number: 18300109
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Neuroscience in general
• Research Institution: Tokyo Medical University
• Principal Investigator: MOCHIDA Sumiko Tokyo Medical University, 医学部, 教授 (30096341)
• Project Period (FY): 2006 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥16,590,000 (Direct Cost: ¥14,100,000、Indirect Cost: ¥2,490,000); Fiscal Year 2008: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2007: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000); Fiscal Year 2006: ¥5,800,000 (Direct Cost: ¥5,800,000)
• Keywords: シナプス / シナプス小胞 / 神経伝達物質 / 開口放出 / シナプス伝達 / エンドサイトーシス

Research Abstract

神経回路可塑性の機能解析、とくにシナプス伝達強化のメカニズムを蛋白分子レベルから解明することを目指し、培養下で交感神経細胞間に形成させたモデルシナプスに、遺伝子や変異遺伝子を導入して外因性蛋白質を強制発現させたり、siRNAを導入して(RNAi法)内因性蛋白発現を抑制し、シナプス小胞の動態を制御して神経伝達物質の放出を短期間、または持続的に増強するシナプス前終末蛋白質群の働きを明らかした。

Research Products

• [Journal Article] Dynamin and activity regulate synaptic vesicle recycling in sympathetic neurons (2009)
  • Author(s): Lu W., Ma, H., Sheng Z.-H. & Mochida S.
  • Journal Title: J Biol Chem. 284 Pages: 1930-1937
  • Peer Reviewed
• [Journal Article] Reciprocal intramolecular interactions of tomosyn control inhibition of the SNARE complex formation (2009)
  • Author(s): Yamamoto, Y., Mochida, S., Kurooka, T. & Sakisaka, T.
  • Journal Title: J Biol Chem. 284(18) Pages: 12480-90
  • Peer Reviewed
• [Journal Article] Dynamin and activity regulate synaptic vesicle recycling in sympathetic neurons. (2009)
  • Author(s): Lu, W.
  • Journal Title: J Biol Chem. 284 Pages: 1930-1937
  • Peer Reviewed
• [Journal Article] Reciprocal intramolecular interactions of tomosyn control inhibition of the SNARE complex formation. (2009)
  • Author(s): Yamamoto, Y.
  • Journal Title: J Biol Chem. (Mar 3. [Epub ahead of print)
  • Peer Reviewed
• [Journal Article] Regulation of presynaptic Ca_v2.1 channels by Ca^<2+> sensor proteins mediates short-term synaptic plasticity (2008)
  • Author(s): Mochida, S., Few, A.P., Scheuer, T. & Catterall, W.
  • Journal Title: Neuron 57 Pages: 210-216
  • Peer Reviewed
• [Journal Article] Dual inhibition of SNARE complex formation by tomosyn ensures controlled neurotransmitter release (2008)
  • Author(s): Sakisaka, T., Yamamoto, Y., Mochida, S., Nakamura, M., Nishikawa, K., Ishizaki, H., Okamoto-Tanaka, M., Miyoshi, J., Fujiyoshi, Y., Manabe, T. & Takai, Y.
  • Journal Title: J Cell Biol. 183(2) Pages: 323-37
  • Peer Reviewed
• [Journal Article] シナプス小胞TRPM7チャネル (2008)
  • Author(s): 持田澄子
  • Journal Title: 蛋白核酸酵素 53(4) Pages: 442-447
  • Peer Reviewed
• [Journal Article] 神経伝達物質の放出機構 (2008)
  • Author(s): 持田澄子
  • Journal Title: 日本生理学雑誌70 Pages: 77-88
  • NAID: 10024918906
• [Journal Article] Dual inhibition of SNARE complex formation by tomosyn ensures controlled neurotransmitter release. (2008)
  • Author(s): Sakisaka, T.
  • Journal Title: J Cell Biol. 183(2) Pages: 23-337
  • Peer Reviewed
• [Journal Article] 神経伝達物質の放出機構 (2008)
  • Author(s): 持田澄子
  • Journal Title: 日本生理学雑誌 70 Pages: 78-86
  • NAID: 10024918906
• [Journal Article] Regulation of presynaptic Ca_v2.1 channels by Ca^<2+> sensor proteins mediates short-term synaptic plasticity (2008)
  • Author(s): Mochida, S.
  • Journal Title: Neuron 57 Pages: 210-216
  • Peer Reviewed
• [Journal Article] CD38 is critical for social behavior by regulating oxytocin secretion (2007)
  • Author(s): Dou Jin, D., Liu, H-X., Hirai, H., Torashima, T., Nagai, T., Lopatina, O., Schayder, N., Yamada, K., Noda, M., Seike, T., Fujita, K., Takasawa, S., Yokoyama, S., Koizumi, K., Shiraishi, M., Tanaka, S., Hashii, M., Yoshihara, T., Higashida, K., Islam, M., Yamada, N., Hayashi, K., Noguchi, N., Kato, I., Okamoto, H., Matsushima, A., Salmina, A., Munesue, T., Shimizu, N., Mochida, S., Asano, M. & Higashida, H.
  • Journal Title: Nature 446 Pages: 41-45
  • Peer Reviewed
• [Journal Article] A cholinergic model synapse to elucidate protein function at presynatic terminals (2007)
  • Author(s): Ma, H. & Mochida S.
  • Journal Title: Neurosci. Res. 57 Pages: 491-498
  • NAID: 10024413713
  • Peer Reviewed
• [Journal Article] A cholinergic model synapse to elucidate protein function at presynatic terminals (2007)
  • Author(s): Ma, H.
  • Journal Title: Neurosci. Res. 57 Pages: 491-498
  • Peer Reviewed
• [Journal Article] 神経伝達 (2007)
  • Author(s): 持田 澄子
  • Journal Title: 高翔 48 Pages: 41-45
• [Journal Article] CD38 is critical for social behavior by regulating oxytocin secretion. (2007)
  • Author(s): Dou Jin, D.
  • Journal Title: Nature 446 Pages: 41-45
• [Journal Article] SAD : a presynaptic kinase associated with synaptic vesicles and the active zone cytomatrix that regulates neurotransmitter release (2006)
  • Author(s): Inoue, E, Mochida, S., Takagi, H., Deguchi-Tawarada, M., Takao-Rikitsu, E., Inoue, M., Yao, I., Setou, M., Ohtsuka, T. & Takai, Y.
  • Journal Title: Neuron 50 Pages: 261-275
  • Peer Reviewed
• [Journal Article] TRPM7 ion channel functions in cholinergic synaptic vesicles and affects transmitter release (2006)
  • Author(s): Krapivinsky, G., Mochida, S., Krapivinsky, L., Cibulsky, S.M. & Clapham, D.E.
  • Journal Title: Neuron 52 Pages: 485-496
  • Peer Reviewed
• [Journal Article] SAD : a presynaptic kinase associated with synaptic vesicles and the active zone cytomatrix that regulates neurotransmitter release. (2006)
  • Author(s): Inoue, E.
  • Journal Title: Neuron 50 Pages: 261-275
• [Journal Article] TRPM7 ion channel functions in cholinergic synaptic vesicles and affects transmitter release. (2006)
  • Author(s): Krapivinsky, G.
  • Journal Title: Neuron 52 Pages: 485-496
• [Presentation] Activity-dependent and-independent synaptic vesicle recycling in sympathetic neurons (2008)
  • Author(s): Lu, W., Ma, H. Sheng, ZH & Mochida, S.
  • Organizer: 39^<th> Annual meeting of the Soc. for Neuroscienc
  • Place of Presentation: Washigton, DC, USA
  • Year and Date: 2008-11-16
• [Presentation] Activity-dependent and -independent synaptic vesicle recycling in sympathetic neurons (2008)
  • Author(s): Mochida, S
  • Organizer: 38^<th> Annual meeting of the Soc. for Neuroscienc
  • Place of Presentation: Washigton, DC
  • Year and Date: 2008-11-16
• [Presentation] Regulation pf Presynaptic Cav2.1 channels by Ca2+ Sensor Proteins Mediates Short-term Synaptic Plasticity (2008)
  • Author(s): Mochida, S
  • Organizer: COE International Symposium 2008 on Development, Learning, Memory and Autism
  • Place of Presentation: 金沢
  • Year and Date: 2008-07-17
• [Presentation] Transpot of mitochondria to presynaptic terminals by syntabulin is required for synaptic transmission (2008)
  • Author(s): Ma, H. & Mochida, S.
  • Organizer: Neuro 2008
  • Place of Presentation: 東京
  • Year and Date: 2008-07-09
• [Presentation] Regulation pf Presynaptic Cav2.1 channels by Ca^<2+> Sensor Proteins Mediates Short-term Synaptic Plasticity (2008)
  • Author(s): Mochida, S., Few, A.P., Scheuer, T. & Catterall, W.
  • Organizer: Gordon Research Conference. Cell Biology of the Neuron
  • Place of Presentation: New London, USA
  • Year and Date: 2008-06-16
• [Presentation] Regulation pf Presynaptic Cav2.1 channels by Ca2+ Sensor Proteins Mediates Short-term Synaptic Plasticity (2008)
  • Author(s): Mochida, S
  • Organizer: Gordon Reshearch Conference Cell Biology of the Neuron
  • Place of Presentation: New London, USA
  • Year and Date: 2008-06-16
• [Presentation] Cav2.1と短期シナプス可塑性 (2008)
  • Author(s): 持田 澄子
  • Organizer: 第85回日本生理学会大会
  • Place of Presentation: 東京
  • Year and Date: 2008-03-26
• [Presentation] シナプス伝達を維持するシナプス前終末への小胞カルゴ蛋白シンタブリン (2008)
  • Author(s): 馬 歓
  • Organizer: 第85回日本生理学会大会
  • Place of Presentation: 東京
  • Year and Date: 2008-03-26
• [Presentation] 長期培養交感神経節細胞におけるエンドサイトーシス (2008)
  • Author(s): 陸 文波
  • Organizer: 第85回日本生理学会大会
  • Place of Presentation: 東京
  • Year and Date: 2008-03-26
• [Presentation] Regulation pf Presynaptic Cav2.1 channels by Ca^<2+> Sensor Proteins Mediates Short-term Synaptic Plasticity (2007)
  • Author(s): Mochida, S., Few, A.P., Scheuer, T. & Catterall, W.
  • Organizer: 38^<th> Annual meeting of the Soc. for Neuroscienc
  • Place of Presentation: San Diego, USA
  • Year and Date: 2007-11-05
• [Presentation] Regulation of presynaptic Ca_v2.1 ch3nnels by Ca^<2+> sellsor proteins mediates short-terms synaptic plasticity (2007)
  • Author(s): Mochida, S.
  • Organizer: 37th Annual meeting of the Soc.for Neuroscience
  • Place of Presentation: サンディエゴ
  • Year and Date: 2007-11-05
• [Presentation] シナプスにおける情報伝達制御 (2007)
  • Author(s): 持田 澄子
  • Organizer: Neuro2007
  • Place of Presentation: 横浜
  • Year and Date: 2007-09-11
• [Presentation] Calcium channels & synaptic plasticity (2007)
  • Author(s): Catterall, A.W.
  • Organizer: Neuro2007
  • Place of Presentation: 横浜
  • Year and Date: 2007-09-11
• [Presentation] Regulation of presynaptic Cav2.1 channels by Ca^<2+> sensor proteins mediates short-term synaptic plasticity (2007)
  • Author(s): Mochida, S.
  • Organizer: International Symposium on Innovative Brain Science for Development, Learning, Memory and Autism
  • Place of Presentation: 金沢
  • Year and Date: 2007-07-17
• [Presentation] The TRPM7 ion channel functions in cholinergic synaptic vesicle exocytosis (2007)
  • Author(s): Mochida, S.
  • Organizer: IBRO2007 Satellite Conference Secretory Vesicle Cycle and Novel Approaches to its Analysis
  • Place of Presentation: ブリスベン
  • Year and Date: 2007-07-10
• [Presentation] Endocytic pathways in the long-cultured sympathetic neuron (2007)
  • Author(s): Lu, W. & Mochida, S.
  • Organizer: 第85回日本生理学会
  • Place of Presentation: 東京
  • Year and Date: 2007-03-26
• [Presentation] Syntabulin, a vesicle cargo-protein, interacting with kinesin-1 maintains synaptic transmission (2007)
  • Author(s): Ma, H. & Mochida, S.
  • Organizer: 第85回日本生理学会
  • Place of Presentation: 東京
  • Year and Date: 2007-03-26
• [Presentation] Presynaptic Cav2.1 channel activity mediates short-term plasticity (2007)
  • Author(s): Mochida, S., Few, AP., Scheuer, T. &. Catterall, WA.
  • Organizer: 第85回日本生理学会
  • Place of Presentation: 東京
  • Year and Date: 2007-03-26
• [Presentation] Neuro2007 Symposia (2006)
  • Author(s): Mochida, S.
  • Organizer: Signal integration at synapses(Organizer)
  • Place of Presentation: 横浜
  • Year and Date: 2006-09-11
• [Presentation] Calcium channels and synaptic plasticity (2006)
  • Author(s): Catterall, W.A. Few A., Lautermilch, N., Scheuer, N. & Mochida, S.
  • Organizer: Neuro 2007 Symposia, Signal integration at synapses
  • Place of Presentation: 横浜
  • Year and Date: 2006-09-11
• [Presentation] The TRPM7 ion channel functions in cholinergic synaptic vesicle exocytosis (2006)
  • Author(s): Mochida, S., Krapivinsky, G. & Clapham, D.E.
  • Organizer: IBRO2007 Satellite Conference, Secretory Vesicle Cycle and Novel Approaches
  • Place of Presentation: Brisbane, Australia
  • Year and Date: 2006-07-10
• [Presentation] Ca^<2+>-dependent regulation of P/Q type Ca^<2+> channel activity : facilitation/depression and calmodulin (2006)
  • Author(s): Mochida, S.
  • Organizer: 第84回日本生理学会大会IUPS symposium : Ca^<2+>-dependent regulation of voltage-gated Ca^<2+> channels
  • Place of Presentation: 大阪
  • Year and Date: 2006-03-21
• [Presentation] Syntabulin, vesicle cargo-protein interacting with kinesin-1, maintains synaptic transmission (2006)
  • Author(s): Ma, H., Lu, W., Itoh, K. & Mochida, S.
  • Organizer: 第84回日本生理学会
  • Place of Presentation: 大阪
  • Year and Date: 2006-03-21
• [Presentation] Molecular mechanisms of presynaptic structure and function (2005)
  • Author(s): Mochida, S.
  • Organizer: Neuroscience 2006 in Kyoto JAPAN Symposia, The latest in presynaptic research : New molecules and physiological functions(Organizer)
  • Place of Presentation: 京都
  • Year and Date: 2005-07-21
• [Presentation] Regulation of SNAREs by tomosyn and its implication in Ca^<2+>-dependent neurotransmitter release (2005)
  • Author(s): Sakisaka, T. Baba, T., Mochida, S., & Takai, Y.
  • Organizer: Neuroscience 2006 in Kyoto JAPAN Symposia, The latest in presynaptic research : New molecules and physiological functions
  • Place of Presentation: 京都
  • Year and Date: 2005-07-21
• [Presentation] Regulation of neurotransmitter release by PKA-phosphorylation of Tomosyn (2005)
  • Author(s): Mochida, S., Sakisaka, T. & Takai, Y.
  • Organizer: Neuro 2006
  • Place of Presentation: 京都
  • Year and Date: 2005-07-21
• [Presentation] Tomosyn phosphorylation by Protein kinase A and its implication in neuro transmitter release Gordon Research Conference (2005)
  • Author(s): Mochida, S., Baba, T., Sakisaka, T. & Takai, Y.
  • Organizer: Cell Biology of the Neuron
  • Place of Presentation: New London, USA
  • Year and Date: 2005-06-21
• [Book] ニューロンの生理学 (2009)
  • Author(s): D. Tritsch (編)
  • Total Pages: 759
  • Publisher: 京都大学学術出版会
• [Book] 蛋白核酸酵素2008年3月号増刊 神経の分化, 回路形成, 機能発現 (2008)
  • Author(s): 持田 澄子
  • Publisher: 共立出版社
• [Book] 翻訳ニューロンの生理学(原著 : D. Tritsch(編))(翻訳分担 : 1章, 17章, 19章)
  • Author(s): 持田澄子, 他
  • Total Pages: 759
  • Publisher: 京都大学学術出版会