Analysis of neural cell adhesion molecule NB-2 involved in the postnatal development of auditory pathways

• Project/Area Number: 18300120
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Neurochemistry/Neuropharmacology
• Research Institution: Nagaoka University of Technology
• Principal Investigator: 渡邉 和忠 Nagaoka University of Technology (70114717)
• Co-Investigator(Kenkyū-buntansha): 霜田 靖 長岡技術科学大学, 工学部, 助教 (00291154)
• Project Period (FY): 2006 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥14,110,000 (Direct Cost: ¥11,800,000、Indirect Cost: ¥2,310,000); Fiscal Year 2008: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000); Fiscal Year 2007: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000); Fiscal Year 2006: ¥4,100,000 (Direct Cost: ¥4,100,000)
• Keywords: 聴覚神経回路 / 細胞接着分子 / 発達 / グルタミン酸 / アポトーシス / シナプス形成 / 聴性脳幹反応 / GABA / グリシン

Research Abstract

神経認識分子NB-2はコンタクチン5とも呼ばれ、聴覚系神経回路で特に顕著に発現している。本研究では、先ずNB-2が音の聞こえ始める直前に発現が強くなり、特に高周波数に反応する領域で発現していることを明らかにした。次に、遺伝子欠損型マウスと野生型マウスで聴覚系神経回路の発達と機能を比較し、遺伝子欠損型マウスでは特に興奮性の神経回路のシナプス部位に異常があり、その結果、一部の神経細胞が細胞死を起こしていること、音に対する脳幹の反応に遅れが出ることを明らかにした。

Research Products

• [Journal Article] Contactins : Emerging key roles in the development and function of the nervous system. (2009)
  • Author(s): Shimoda, Y. and Watanabe, K.
  • Journal Title: Cell Adh. Migr. 3(1) Pages: 64-70
• [Journal Article] Preferential localization of neural cell recognition molecule NB-2 in developing glutamatergic neurons in the rat auditory brainstem (2009)
  • Author(s): Toyoshima, M., Sakurai, K., Shimazaki, K., Takeda, Y., Nakamoto, M., Serizawa, S., Shimoda, Y. and Watanabe, K
  • Journal Title: J. Comp. Neurol. 513(4) Pages: 349-362
• [Journal Article] Contactin-associated protein (Caspr) 2 interacts with carboxypeptidase E in the central nervous system. (2009)
  • Author(s): Oiso, S., Takeda, Y., Futagawa, T., Miura, T., Kuchiiwa, S., Nishida, K., Ikeda, R., Kariyazono, H., Watanabe, K. and Yamada, K.
  • Journal Title: J. Neurochem. 109(1) Pages: 158-167
• [Journal Article] Preferential Localization of Neural Cell Recognition Molecule NB-2 at Developing Glutamatergic Presynapses from Tonotopic High-Frequency Regions in the Rat Auditory Brainstem. (2009)
  • Author(s): Toyoshima, et al.
  • Journal Title: J. Comp. Neurol. 513 Pages: 349-362
  • Peer Reviewed
• [Journal Article] Contactins : Emerging key roles in the development and function of the nervous system. (2009)
  • Author(s): Shimoda, Watanabe
  • Journal Title: Cell Adhesion and Migration 3 Pages: 1-7
  • Peer Reviewed
• [Journal Article] Contactin-associated protein (Caspr)2 interacts with carboxypeptidase E in the central nervous system. (2009)
  • Author(s): Oiso, et al.
  • Journal Title: J. Neurochem. 109 Pages: 158-167
  • Peer Reviewed
• [Journal Article] Impairment of learning and memory in TAG-1 deficient mice associated with shorter CNS internodes and disrupted juxtaparanodes. (2008)
  • Author(s): Savvaki, M., Panagiotaropoulos, T., Stamatakis, A., Sargiannidou, I., Karatzioula, P., Watanabe, K., Stylianopoulou, F., Karagogeos, D. and Kleopa, K.A.
  • Journal Title: Mol. Cell. Neurosci. 39(3) Pages: 478-490
• [Journal Article] Structural requirement of TAG-1 for retinal ganglion cell axons and myelin in the mouse optic nerve. (2008)
  • Author(s): Chatzopoulou, E., Miguez, A., Savvaki, M., Levasseur, G., Muzerelle, A., Muriel, M.P., Goureau, O., Watanabe, K., Fisher, G., Goutebroze, L., Gaspar, P., Zalc, B., Karagogeos, D. and Thomas, J.L.
  • Journal Title: J. Neurosci. 28(30) Pages: 7624-7636
• [Journal Article] A TAG1-APP signaling pathway through Fe65 negatively modulates neurogenesis. (2008)
  • Author(s): Ma, Q.-H., Yang, W.-I., Futagawa, T., Jiang, X.-D., Takeda, Y., Xu, R.-X., Bagnard, D., Schachner, M., Furley, A.J., Karagogeos, D., Watanabe, K., Dawe, G.S. and Xiao, Z.-C.
  • Journal Title: Nature Cell Biol. 10(3) Pages: 283-294
• [Journal Article] Neural recognition molecule CHL1 and NB-3 regulate apical dendrite orientation in neocortex via PTPα (2008)
  • Author(s): Ye, H., Tan, Y.L.J., Ponniah, S., Takeda, Y., Wang, S.-Q., Schachner, M., Watanabe, K., Pallen, C.J. and Xiao, Z.-C.
  • Journal Title: EMBO J. 27(1) Pages: 188-200
• [Journal Article] Structural requirement of TAG-1 for retinal ganglion cell axons and myelin in the mouse optic nerve. (2008)
  • Author(s): Chatzopoulou, et al.
  • Journal Title: J. Neurosci. 28 Pages: 7624-7636
  • Peer Reviewed
• [Journal Article] Impairment of learning and memory in TAG-1 deficient mice associated with shorter CNS internodes and disrupted iuxtaparanodes. (2008)
  • Author(s): Savvaki, et al.
  • Journal Title: Mol. Cell Neurosci. 39 Pages: 478-490
  • Peer Reviewed
• [Journal Article] Neural Recognition Molecule CHL1 and NB-3 Regulate Apical Dendrite Orientation in Neocortex via PTP α. (2008)
  • Author(s): Ye, et. al.
  • Journal Title: EMBO J 27 Pages: 188-200
  • Peer Reviewed
• [Journal Article] A TAG1-APP signaling pathway through Fe65 negatively modulates neurogenesis. (2008)
  • Author(s): Ma, et. al.
  • Journal Title: Nature Cell Biol. 10 Pages: 283-294
  • Peer Reviewed
• [Journal Article] Oligodendrocytes regulate formation of nodes of Ranvier via the recognition molecule Omgp (2006)
  • Author(s): Nie, D.-Y., Ma, Q.-H., Law, J.W.S., Chia, C.-P., Dhingra, N.K., Xu, G., Shimoda, Y., Gong, N., Chen, Q.-W., Hu, Q.-D., Chow, P.K.H., Ng, Y.-K., Ling, E.-A., Watanabe, K., Xu, T.-L., Schachner, M. and Xiao, Z.-C.
  • Journal Title: Neuron Glia Biol. 2 Pages: 151-164
• [Journal Article] Oligodendrocytes regulate formation of nodes of Ranvier via the recognition molecule OMgp. (2006)
  • Author(s): Nie, D.Y.et al.
  • Journal Title: Neuron Glia Biol. 2 Pages: 151-164
• [Presentation] NB-2欠損マウスにおける聴性脳幹反応(ABR)の変化とグルタミン酸作動性シナプスの形成異常 (2008)
  • Author(s): 豊島学、桜井都衣、武田泰生、島崎久仁子、霜田靖、渡邉和忠
  • Organizer: 第51回日本神経化学会大会
• [Presentation] 神経認識分子NB-2の欠損がマウス聴覚系に与える影響 (2008)
  • Author(s): 豊島学、桜井都衣、島崎久仁子、霜田靖、渡邉和忠
  • Organizer: 第31回日本神経科学大会
• [Presentation] NB-2欠損マウスにおける聴性脳幹反応(ABR)の変化とグルタミン酸作動性シナプスの形成異常 (2008)
  • Author(s): 豊島ら
  • Organizer: 第51回日本神経化学会大会
  • Place of Presentation: 富山国際会議場
• [Presentation] 神経認識分子NB-2の欠損がマウス聴覚系に与える影響 (2008)
  • Author(s): 豊島ら
  • Organizer: 第31回日本神経科学大会
  • Place of Presentation: 東京フォーラム
• [Presentation] Neural recognition molecule NB-2 in the auditory brainstem (2007)
  • Author(s): Toyoshima, et. al.
  • Organizer: 37th Annual Meeting, Society for Neuroscience
  • Place of Presentation: San Diego
  • Year and Date: 2007-11-04
• [Presentation] Neural cell recognition molecule NB-3 in the developing mouse cerebellum (2007)
  • Author(s): Sakurai, et. al.
  • Organizer: 37th Annual Meeting, Society for Neuroscience
  • Place of Presentation: San Diego
  • Year and Date: 2007-11-03
• [Presentation] Neural recognition molecule NB-2 in the auditory brainstem (2007)
  • Author(s): Toyoshima, M., Okumura, T., Sakurai, K., Shimazaki, K., Shimoda, Y. and Watanabe, K.
  • Organizer: 37th Annual Meeting, Society for Neuroscience
  • Place of Presentation: San Diego, USA
• [Presentation] Neural cell recognition molecule NB-3 in the developing mouse cerebellum (2007)
  • Author(s): Sakurai, K., Suzuki, Y., Takeda, Y., Shimoda, Y., Karagogeos, D. and Watanabe, K.
  • Organizer: 37th Annual Meeting, Society for Neuroscience
  • Place of Presentation: San Diego, USA
• [Presentation] 聴覚系の上オリーブ核群におけるNB-2の発現 (2007)
  • Author(s): 豊島学、桜井都衣、島崎久仁子、霜田靖、渡邉和忠
  • Organizer: Neuro 2007(第30回日本神経科学大会、第50回日本神経化学会大会、第17回日本神経回路会大会)
• [Presentation] Expression of NB-2 in GABAergic neurons of the rat auditory system (2006)
  • Author(s): Toyoshima, M., Takagi, M., Takeda, Y., Shimoda, Y. and Watanabe, K
  • Organizer: 36th Annual Meeting, Society for Neuroscience
  • Place of Presentation: Atlanta, USA
• [Presentation] ラット聴覚系のGABA 作動性ニューロンにおけるNB-2の発現 (2006)
  • Author(s): 豊島学、武田泰生、霜田靖、渡邉和忠
  • Organizer: 第29回日本神経科学大会