Production of basic intestinal microflora for SPF mice using development of lesions of human desease modele mice as an indicator.

• Project/Area Number: 18300138
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Laboratory animal science
• Research Institution: The University of Tokyo
• Principal Investigator: ITOH Kikuji The University of Tokyo, 大学院・能楽生命科学研究科, 准教授 (50100045)
• Co-Investigator(Kenkyū-buntansha): HIRAYAMA Kazuhiro 東京大学, 大学院・能楽生命科学研究科, 助教 (60208858) ; URANO Touru 熊本大学, 生命科学研究科, 教授 (90101899) ; TAKAKURA Akira (財)実験動物中央研究所, 室長(研究職) (60167484)
• Project Period (FY): 2006 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥17,540,000 (Direct Cost: ¥15,500,000、Indirect Cost: ¥2,040,000); Fiscal Year 2008: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000); Fiscal Year 2007: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000); Fiscal Year 2006: ¥8,700,000 (Direct Cost: ¥8,700,000)
• Keywords: 腸内フローラ / 病態モデル / SPFマウス / モニタリング / 病態モデル動物 / モニタリンガ / 病体モデス動物

Research Abstract

Identification of intestinal bacteria related to the development of lesions in Δra (rheumatoid model), AID (IgA production failure model) and IL-10 KO (IBD model) mice, and development of monitoring system for mouse intestinal flora using FISH.

Research Products

• [Journal Article] Bacteroides induce higher IgA production than Lactobacillus by increasing activation-induced cytidine deaminase expression in B cells in murine peyer's patches (2009)
  • Author(s): Yanagibashi, T., Hosono, A., Oyama, A., Tsuda, M., Hachimura, S., Takahashi, Y.,_Itoh, K., Hiravama, K., Takahashi, K., Kaminogawa, S.
  • Journal Title: Biosci. Biotechnol. Biochem 73 Pages: 372-377
• [Journal Article] Intestinal Bifidobacterium association in germ-free T cell receptor transgenic mice down-regulates dietary antigen-specific immune responses of the small intestine but enhances those of large intestine (2009)
  • Author(s): Tsuda, M., Hosono, A., Yanagibashi, T., Hachimura, S., Hirayama, K., Umesaki, Y., Itoh, K., Takahashi, K., Kaminogawa, S.
  • Journal Title: Immunobiology 214 Pages: 279-289
• [Journal Article] Induction of intestinal Th17 cells by segmented filamentous bacteria (2009)
  • Author(s): Inanov, I. I., Atarashi, K., Manel, N., Brodie, E., Shima, T., Karaoz, U., Wei, D., Goldfarb, K. C. Santee, C. A., Lynch, S. V., Tanoue, T., Imaoka, A., Itoh, K., Takeda, K., Umesaki, Y., Honda, K., Litteman, D. R.
  • Journal Title: Cell 139 Pages: 485-498
• [Journal Article] Uptake through glycoprotein 2 of FimH^+ bacteria by M cells initiates mucosal immune response (2009)
  • Author(s): Hase, K., Kawano, K., Nochi, T., Pontes, GS, Fukuda, S., Ebisawa, M., Kadokura, K., Tobe, T., Fujimura, Y., Kawano, S., Yabashi, A., Waguri, S., Nakato, G, Kimura, S., Murakami, T., limura, M., Hamura, K., Fukuoka, S., Lowe, AW, Itoh, K. Kiyono, H., Ohno, H.
  • Journal Title: Nature 462 Pages: 226-230
• [Journal Article] 16S rRNA gene sequence-based analysisi of clostridia related to conversion of germfree mice to the normal state (2009)
  • Author(s): Momose, Y., Maruyama, A., Iwasaki, T., Miyamoto, Y., Itoh, K.
  • Journal Title: J. Appl. Microbiol 107 Pages: 2088-2097
• [Journal Article] Induction of intestinal Th17 cells by segmented filamentous bacteria (2009)
  • Author(s): Inanov, I.I., Atarashi, K., Honda, K.Itoh, K., 他
  • Journal Title: Cell 139 Pages: 485-498
  • Peer Reviewed
• [Journal Article] Uptake through glycoprotein 2 of FimH^+ bacteria by M cells initiates mucosal immune response (2009)
  • Author(s): Hase, K., Kawano, K., Itoh, K., 他
  • Journal Title: Nature 462 Pages: 226-230
  • Peer Reviewed
• [Journal Article] 16S rRNA gene sequence-based analysisi of clostridia related to conversion of germfree mice to the normal state (2009)
  • Author(s): Momose, Y., Itoh, K., 他
  • Journal Title: Journal of Applied Microbiology 107 Pages: 2088-2097
  • Peer Reviewed
• [Journal Article] Effect of organic acids on inhibition of Escherichia coli O157 : H7 colonization in gnotobiotic mice associated with infantintestinal microbiota (2008)
  • Author(s): Momose Y., Hiravama K., Itoh, K.
  • Journal Title: Antonie van Leeuwenhoek 93 Pages: 141-149
• [Journal Article] Competition for ploline between indigenous Escherichia coli, E. coli O157 : H7 in gnotobiotic mice associated with infant intestinal microbiota and its contribution to the colonization resistance against E. coli O157 : H7 (2008)
  • Author(s): Momose, Y., Hirayama, K., Itoh, K.
  • Journal Title: Antonie van Leeuwenhoek 94 Pages: 165-171
• [Journal Article] Requirement for Lymphoid Tissue-inducer cells in isolated follicle formation and Tcell-independent immunoglobulin A generation in the gut (2008)
  • Author(s): Tsuji, M., Suzuki, K., Kitamura, H., Maruya, M., Kinoshita, K., Ivanov, I. I., Itoh, K., Littman, D. R., Fagarasan, S.
  • Journal Title: Immunity 29 Pages: 261-271
• [Journal Article] Activation- induced cytidine deaminase deficiency causes organ-specific autoimmune disease (2008)
  • Author(s): Hase, K., Takahashi, D., Ebisawa, M., Kawano, S., Itoh, K., Ohno, H.
  • Journal Title: PloS ONE 3
• [Journal Article] Activation-induced cytidine deaminase deficiency causes organ-specific autoimmune disease (2008)
  • Author(s): Hase, K., Takahashi, D., Ebisawa, M., Kawano, S., Itoh, K., Ohno, H.
  • Journal Title: PLoS ONE 3 Pages: 1-10
  • Peer Reviewed
• [Journal Article] Comparative genome analysis of Lactobacillus reuteri and Lactobacillus fermentum Reveal a genomic island for reuterin and cobalamin production (2008)
  • Author(s): Morita, H., Kato, Y., Takizawa, T., Fukuoka, H., Yoshimura, T., Itoh, K., 他
  • Journal Title: DNA Reaearch 15 Pages: 151-161
  • Peer Reviewed
• [Journal Article] Prior stimulation of antigen-presenting cells with Lactobacillus regulates excessive antigen-specific cytokine responses in vitro when compared with Bacteroides (2008)
  • Author(s): Tsuda, M., Hosono, A., Yanagibashi, T., Hachimura, S., Hirayama, K., Itoh, K., Takahashi, K., Kaminogawa, S.
  • Journal Title: Cytotechnology 55 Pages: 89-101
  • Peer Reviewed
• [Journal Article] (2008)
  • Author(s): 伊藤喜久治
  • Journal Title: 胃腸管の微生物とプロバイオティクス 食品機能性の科学(産業技術サービスセンター) Pages: 426-432
• [Journal Article] Prior stimulation of antigen-presenting cells with Lactobacillus regulates excessive antigen-specific cytokine responses in vitro when compared with Bacteroides (2007)
  • Author(s): Tsuda, M., Hosono, A., Yanagibashi, T., Hachimura, S., Hiravama, K., Itoh, K., Takahashi, K., Kaminogawa, S.
  • Journal Title: Cytotechnology 55 Pages: 89-101
• [Journal Article] Production of shiga toxin by a luxS mutant of Echerichia coli0157: H7 in vivo and in vitro. (2007)
  • Author(s): Jeon, B. & Itoh, K.
  • Journal Title: Microbiol. Immunol. 51(4) Pages: 391-396
• [Journal Article] Vitamin K deficiency of germfree mice caused by feeding standard purified diet sterilized by γirradiation. (2007)
  • Author(s): Hirayama, K., Uetsuka, K., Kuwabara, Y., Tamura, M. & Itoh, K.
  • Journal Title: Exp. Anim. 56(4) Pages: 273-278
  • NAID: 10019531978
• [Journal Article] Discrimination between Campylobacter jejuni and C.coli using a macrolide and quinolone line probe assey (MQ-LiPA) (2007)
  • Author(s): Niwa, H., Asai, Y., Yamai, S. & Itoh, K.
  • Journal Title: Bioscience Microflora 26(3) Pages: 71-74
• [Journal Article] Biotransformation of bile acids by bacterioroides sp. strain T-40 isolated from human microflora. (2007)
  • Author(s): Ogura, Y., Takei, T., Suzuki, T., Yamaga, N., Itoh, K., Yamada, K. & Uchida, K.
  • Journal Title: Yonago Acta medica 50(2) Pages: 33-40
• [Journal Article] An improved DNA isolation method for metagenbmic analysis of the microbial flora of the human intestine. (2007)
  • Author(s): Norita, H., Kuwahara, T., Ohshima, K. Sasamoto, H., Itoh, K., Hattori, M., Hayashi, T, & Takami, H.
  • Journal Title: Microbes Environ. 22(3) Pages: 214-222
• [Journal Article] Effect of organic acids on inhibition of Echerichia coli 0157: H7 colonization in gnotobiotic mice associated with infant intestinal microbiota.
  • Author(s): Momose, Y. Hirayama, K. & Itoh, K.
  • Journal Title: Antonie van Leeuwenhoek (in press)
• [Presentation] Design of group-specific oligonucleotide probes for the detection of mouse clostridial groups and their application to the analysis of mouse fecal microbiota by fluorescence in situ hybriddization (2010)
  • Author(s): Momose, Y., Itoh, K., 他
  • Organizer: 2^<nd> TNO Beneficial Microbes Conference
  • Place of Presentation: NH Conference Centre, The Netherlands
  • Year and Date: 2010-03-16
• [Presentation] Design of group-specific oligonucleotide probes for the detection of mouse clostridial groups and their application to the analysis of mouse fecal microbiota by fluorescence in situ hybridization (2010)
  • Author(s): Momose, Y., Itoh, K., 他
  • Organizer: 2^<nd> TNO Beneficial Microbes Conference
  • Place of Presentation: NH Conference Centre, The Netherlands
  • Year and Date: 2010-03-16
• [Presentation] 腸内フローラと腸内病原菌 (2008)
  • Author(s): 伊藤喜久治
  • Organizer: 第12回腸内細菌学会
  • Place of Presentation: 東京大学弥生講堂
  • Year and Date: 2008-06-13
• [Book] 食品機能性の科学、西川研次郎監修(胃腸管の微生物とプロバイオティクス) (2008)
  • Author(s): 伊藤喜久治
• [Book] 微生物増殖学の現在・未来 福井作蔵・秦野琢之編・監修(腸管における細菌増殖) (2008)
  • Author(s): 伊藤喜久治
• [Book] 腸内フローラとプロバイオティクス乳酸菌 乳酸菌の保健機能と応用(上野川修一監修) (2007)
  • Author(s): 伊藤喜久治