Study for molecular basis of AIDS pathogenesis on non-human primate model

• Project/Area Number: 18300142
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Laboratory animal science
• Research Institution: Kyoto University
• Principal Investigator: MIURA Tomoyuki Kyoto University, ウイルス研究所, 准教授 (40202337)
• Co-Investigator(Kenkyū-buntansha): IDO Eiji 京都大学, ウイルス研究所, 特別教育研究准教授 (70183176)
• Co-Investigator(Renkei-kenkyūsha): IDO Eiji 京都大学, ウイルス研究所, 特別教育研究准教授 (70183176)
• Project Period (FY): 2006 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥18,100,000 (Direct Cost: ¥15,400,000、Indirect Cost: ¥2,700,000); Fiscal Year 2008: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000); Fiscal Year 2007: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000); Fiscal Year 2006: ¥6,400,000 (Direct Cost: ¥6,400,000)
• Keywords: 感染症 / ウイルス / エイズ / アカゲザル / 病原性 / 病理学 / 微生物 / 獣医学

Research Abstract

(1)強毒/弱毒SHIV感染初期に見られる胸腺内の未成熟なT細胞の分化増殖能の差異が、その後の病態進行を反映することが示唆された。(2)SHIVの病原性に影響を及ぼすウイルスゲノム上の変異がウイルス産生能と感染力の2つの素過程として同定された。(3)強毒/弱毒SHIVは感染初期において全身への拡散速度とCD4+細胞傷害作用が大きく異なり、この差異が病態進行に関与する事が示唆された。

Research Products

• [Journal Article] Construction and infection of a new simian/human immunodeficiency chimeric virus (SHIV) containing the integrase gene of the human immunodeficiency virus type 1 genome and analysis of its adaptation to monkey cells (2008)
  • Author(s): Akiyama,H., 他
  • Journal Title: Microbes and Infection 10 Pages: 531-539
  • Peer Reviewed
• [Journal Article] SmallintestinCD4+T-cellsare profoundly depleted during acute infection of simian-human immunodeficiency virus regardless of its pathogenicity (2008)
  • Author(s): Fukazawa,Y., 他
  • Journal Title: J.Virol 82 Pages: 6039-6044
  • Peer Reviewed
• [Journal Article] Small intestine CD4+T-cells are profoundly depleted during acute infection of simian-human immunodeficiency virus regardless of its pathogenicity. (2008)
  • Author(s): Fukazawa, Y., et al.
  • Journal Title: J. Virol 82 Pages: 6039-6044
  • Peer Reviewed
• [Journal Article] Construction and infection of a new simian/human immunodeficiency chimeric virus (SHIV)containing the integrase gene of the human immunodeficiency virus type 1 genome and analysis of its adaptation to monkey cells. (2008)
  • Author(s): Akiyama, H., et al.
  • Journal Title: Microbes and Infection 10 Pages: 531-539
  • Peer Reviewed
• [Journal Article] Trans-species activation of human T cells by rhesus macaque CD1b molecules (2008)
  • Author(s): Morita, D., et al.
  • Journal Title: BBRC 377 Pages: 889-893
  • Peer Reviewed
• [Journal Article] Construction of a novel SHIV having an HIV-l-derived protease gene and its infection to rhesus macaques : a useful tool for in vivo efficacy tests of protease inhibitors (2007)
  • Author(s): lshimatsu,M., 他
  • Journal Title: Microbes and Infection 9 Pages: 475-482
  • Peer Reviewed
• [Journal Article] Construction of a novel SHIV having an HIV-1-derived protease gene and its infection to rhesus macaques: a useful tool for invivo efficacy tests of protease inhibitors (2007)
  • Author(s): Ishimatsu, M., et. al.
  • Journal Title: Microbes and Infection 9 Pages: 475-482
  • Peer Reviewed
• [Journal Article] A genetically engineered live-attenuated simian-human immunodeficiency virus that co-expresses the RANTES gene improves the magnitude of cellular immunity in rhesus macaques (2007)
  • Author(s): Shimizu, Y., et. al.
  • Journal Title: Virology 361 Pages: 68-79
  • Peer Reviewed
• [Journal Article] Contribution of monocytes to viral replication in macaques during acute infection with simian immunodeficiency virus (2007)
  • Author(s): Kuwata, T., et. al.
  • Journal Title: AIDS Res. Hum. Retroviruses 23 Pages: 372-380
  • Peer Reviewed
• [Journal Article] Rapid dissemination of a pathogenic simian/human immunodeficiency virus to systemic organs and active replication in lymphoid tissues following intrarectal infection (2006)
  • Author(s): Miyake,A., 他
  • Journal Title: J. Gen. Virol 87 Pages: 1311-1320
  • Peer Reviewed
• [Journal Article] Impaired T cell differentiation in the thymus at the early stages of acute pathogenic SHIV infection in contrast to less pathogenic SHIV infection (2006)
  • Author(s): Motohara,M., 他
  • Journal Title: Microbes and Infection 8 Pages: 1539-1549
  • Peer Reviewed
• [Journal Article] Impaired T cell differentiation in the thymus at the early stages of acute pathogenic SHIV infection in contrast to less pathogenic SHIV infection. (2006)
  • Author(s): Motohara, M. et al.
  • Journal Title: Microbes and Infection 8 Pages: 1539-1549
• [Journal Article] Rapid dissemination of a pathogenic simian/human immunodeficiency virus to systemic organs and active replication in lymphoid tissues following intrarectal infection. (2006)
  • Author(s): Miyake, A. et al.
  • Journal Title: Journal of General Virology 87 Pages: 1311-1320
• [Journal Article] DNA vaccination of macaques by a full-genome SHIV plasmid that has an IL-2 gene and Produces non-infectious virus particles. (2006)
  • Author(s): Horiuchi, R. et al.
  • Journal Title: Vaccine 24 Pages: 3677-3685
• [Journal Article] Construction and in vitro characterization of a chimeric simian and human immunodeficiency virus with the RANTES gene. (2006)
  • Author(s): Shimizu, Y. et al.
  • Journal Title: Microbes and Infection 8 Pages: 105-113
• [Journal Article] Whole genome sequence data of an infectious molecular clone of the SIVagm TYO-1 strain. (2006)
  • Author(s): Miura, T. et al.
  • Journal Title: AIDS Res. Hum. Retroviruses 22 Pages: 1183-1185
• [Presentation] SHIV-KS661長期感染アカゲサルにおけるウイルス増殖、CD4陽性T細胞の減少およびEnteropathyについて (2008)
  • Author(s): 稲葉一寿、深澤嘉伯、堀内励生、松田健太、姫野愛、松山めぐみ、伊吹謙太郎、速水正憲、五十嵐樹彦、三浦智行
  • Organizer: 第56回日本ウイルス学会学術集会
  • Place of Presentation: 岡山
  • Year and Date: 2008-10-26
• [Presentation] IIIV-1由来のpol遺伝子を持つSHIV-prtiのアカゲザルin vivo継代による感染増殖能の増加 (2008)
  • Author(s): 井戸栄治、石松美沙、三浦智行、多田哲子、多田秀子、伊吹謙太郎
  • Organizer: 第22回日本エイズ学会学術集会
  • Place of Presentation: 大阪
• [Presentation] IIIV-1の逆転写酵素とインテグラーゼ遺伝子を持つSHIV-rtiはアカゲザルに持続感染しエイズ様症状を引き起こす (2008)
  • Author(s): 井戸栄治、石松美沙、三浦智行、多田哲子、多田秀子、伊吹謙太郎
  • Organizer: 第56回日本ウイルス学会学術集会
  • Place of Presentation: 岡山
• [Presentation] Investigation of acute pathogenesis of simian and human immunodeficiency chimeric virus by comparison of its molecular clones with different pathogenic abilities. (2008)
  • Author(s): Miura, T.
  • Organizer: The Second China-Japan Colloquium of Mathematical Biology
  • Place of Presentation: Okayama
• [Presentation] 〜エイズの標的組織は小腸である〜霊長類エイズモデルの全身深部組織解析から見えてきたこと (2007)
  • Author(s): 三浦智行, 他
  • Organizer: 第143回日本獣医学会学術集会
  • Place of Presentation: つくば市(つくば国際会議場)
  • Year and Date: 2007-04-03
• [Presentation] SIVmacにHIV-1のプロテアーゼ遺伝子を組み込んだSHIV-prのin vivo継代によって生じた遺伝子変異の解析 (2007)
  • Author(s): 石松美沙、鈴木元、秋山尚志、三浦智行、速水正憲、井戸栄治
  • Organizer: 第21回日本エイズ学会学術集会
  • Place of Presentation: 広島
• [Presentation] プロテアーゼ、逆転写酵素、およびインテグラーゼの各遺伝子がHIV-1由来であるSHIV-prtiのサルにおけるin vivo継代 (2007)
  • Author(s): 井戸栄治、石松美沙、三浦智行
  • Organizer: 第21回日本エイズ学会学術集会
  • Place of Presentation: 広島
• [Presentation] Virological and immunological analysis of systemic lymphoid tissues in SHIV-infected monkeys (2007)
  • Author(s): Ibuki, K., Inaba, K., Fukazawa, Y., Matsuyama, M., Hayami, M., Miura, T.
  • Organizer: 25th annual Symposium on Nonhuman Primate Models for AIDS
  • Place of Presentation: Monterey, U.S.A
• [Presentation] エイズの標的組織は小腸である一霊長類エイズモデルの全身深部組織解析から見えてきたこと (2007)
  • Author(s): 三浦智行、稲葉一寿、深澤嘉伯、松山めぐみ、伊吹謙太郎、速水正憲
  • Organizer: 第143回日本獣医学会学術集会
  • Place of Presentation: つくば
• [Presentation] プロテアーゼ、逆転写酵素、およびインテグラーゼの各遺伝子がHIV-1由来である新規SHIVのサル感染実験 (2006)
  • Author(s): 井戸栄治、石松美沙、速水正憲、三浦智行
  • Organizer: 第20回日本エイズ学会学術集会
  • Place of Presentation: 東京
• [Presentation] 強毒SHIV感染サルの全身臓器におけるウイルス変異の解析 (2006)
  • Author(s): 松山めぐみ、堀内励生、深澤嘉伯、稲葉一寿、伊吹謙太郎、速水正憲、三浦智行
  • Organizer: 第54回日本ウイルス学会学術集会
  • Place of Presentation: 名古屋
• [Presentation] Virological and immunopathological analysis of systemic lymphoid tissues in SHIV-infected monkeys : Importance of small intestine as target organ of AIDS (2006)
  • Author(s): Miura, T
  • Organizer: The 7th Kumamoto AIDS Seminar
  • Place of Presentation: Kumamoto
• [Presentation] Even low pathogenic SHIV lead to decrease CD4+ T-cell of the small intestine significantly from early phase of infection (2004)
  • Author(s): Fukazawa, Y., Miyake, A., Ibuki, K., Inaba, K., Saito, N., Motohara, M., Horiuchi, R., Himeno, A., Matsuda, K., Nakamura, M., Matsuyama, M., Hayami, M., Miura, T
  • Organizer: 24th annual Symposium on Nonhuman Primate Models for AIDS
  • Place of Presentation: Atlanta