• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Development of Bioprobe Integrated with Microneedle for Cellular Function Analysis

Research Project

Project/Area Number 18300150
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Biomedical engineering/Biological material science
Research InstitutionToyohashi University of Technology

Principal Investigator

SHIBATA Takayuki  Toyohashi University of Technology, Faculty of Engineering, Associate Professor (10235575)

Co-Investigator(Kenkyū-buntansha) MAKINO Eiji  Hirosaki University, Faculty of Science and Technology, Professor (70109495)
MASUZAWA Toru  Ibaraki University, Faculty of Engineering, Professor (40199691)
KAWASHIMA Takahiro  Toyohashi University of Technology, Faculty of Engineering, Assistant Professor (50378270)
Project Period (FY) 2006 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥16,410,000 (Direct Cost: ¥15,600,000、Indirect Cost: ¥810,000)
Fiscal Year 2007: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2006: ¥12,900,000 (Direct Cost: ¥12,900,000)
KeywordsMicroneedle / Atomic force microscope(AFM) / Cellular function analysis / MEMS / BioMEMS / Micromachining / Cell surgery / Nanobiotechnology
Research Abstract

In order to realize cellular function analysis on a chip-base system, we have developed a newly designed AFM probe in which a conventional sharp tip is superseded by a hollow silicon dioxide(SiO_2) microneedle connecting the root to a fluidic microchannel embedded into a silicon(Si) cantilever beam structure. The probe will be capable not only of performing AFM measurements but also of introducing desired biomolecules(nucleic acids, proteins, etc.) into living cells and extracting biomolecules expressed in the cells.
The micromachining technique of a hollow SiO_2 microneedle with a sharpened tip has been developed on the basis of a combination of an anisotropic Deep reactive ion etching(DRIE) process for producing thorough holes into a Si substrate as a needle mold followed by wet oxidation and a selective etching process for leaving a SiO_2 microneedle structure, resulting that microneedles with a tip diameter of less than 0.1 μm(5.5 μm in outer diameter and 20 μm in length) was succes … More sfully fabricated. A circular-shaped microchannel(typically 6 μm in diameter) was also successfully fabricated into a Si cantilever structure. In additon, a novel electrostatic field-assisted direct bonding technique was developed.
In order to investigate the mechanical stability of a microneedle array, penetration tests were performed with a gelatin as an artificial cell. The results indicated that the microneedles were expected to be sufficiently stiff to penetrate living cells without fracture. Insertion performance into gelatin and the possibility of liquid delivery with fabricated microneedles were also investigated. The displacement required for needle insertion increased linearly with an increase in surface area at the needle tip. It was also found that increasing mechanical oscillation frequency during insertion decreased remarkably the displacement probably due to an increase in viscous resistance. Moreover, the fluid ejection tests revealed the possibility of liquid delivery by employing fabricated microneedles with an inner diameter as small as 3.5 μm. It was also founded that the flow rate increased in proportion to the fourth power of the inner diameter of the needles that is subject to the Hagen-Poiseuille's equation. Less

Report

(3 results)
  • 2007 Annual Research Report   Final Research Report Summary
  • 2006 Annual Research Report
  • Research Products

    (29 results)

All 2008 2007 2006 Other

All Journal Article (12 results) (of which Peer Reviewed: 4 results) Presentation (15 results) Patent(Industrial Property Rights) (2 results)

  • [Journal Article] Fabrication and fluid ejection performance of microneedle array for cellular function analysis2008

    • Author(s)
      T. Shibata, N. Kato, T. Sakai, T. Kawashima, M. Nomura, T. Mineta, E. Makino
    • Journal Title

      Proc. 3rd JSME/ASME International Conference on Materials and Processing(ICM&P 2008) (印刷中)

      Pages: 4-4

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Insertion performance of hollow microneedle array for cell surgery2008

    • Author(s)
      T. Kawashima, T. Shibata, T. Sakai, N. Kato, M. Nomura, T. Mineta, E. Makino
    • Journal Title

      Proc. Asia-Pacific Conference on Transducers and Micro-Nano Technology 2008(APCOT2008) (印刷中)

      Pages: 4-4

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Fabrication and mechanical characterization of microneedle array for cell surgery2007

    • Author(s)
      T. Shibata, A. Nakanishi, T. Sakai, N. Kato, T. Kawashima, T. Mineta, E. Makino
    • Journal Title

      Proc. 14th International Conference on Solid-State Sensors, Actuators and Microsystems(Transducers'07) Volume1

      Pages: 719-722

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Development of MEMS devices for cell surgery and cellular function analysis on a chip-based system (in Japanese)2007

    • Author(s)
      N. Kato, T. Shibata, T. Sakai, S. Yamanaka, T. Kawashima
    • Journal Title

      The Papers of Technical Meeting on Linear Drives, IEE Japan LD-07-49

      Pages: 89-92

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Fabrication and mechanical characterization of microneedle array for cell surgery2007

    • Author(s)
      T. Shibata, A. Nakanishi, T. Sakai, N. Kato, T. Kawashima, T. Mineta, E. Makino
    • Journal Title

      Proc. of the 14th International Conference on Solid-State Sensors, Actuators and Microsystems (Transducers '07) 1

      Pages: 719-722

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Fabrication and mechanical characterization of microneedle array for cell surgery2007

    • Author(s)
      T. Shibata, A.Nakanishi, T. Sakai, N. Kato, T. Kawashima, T. Mineta, E. Makino
    • Journal Title

      Proc.14th International Conference on Solid-State Sensors, Actuators and Microsystems(Transducers'07) Volume 1

      Pages: 719-722

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 細胞機能解析のためのマイクロニードル搭載型バイオプローブの開発(第1報)-先鋭化ニードルおよびマイクロチャネルの作製-2007

    • Author(s)
      加藤統久, 柴田隆行, 川島貴弘, 堀内 宰, 峯田 貴, 牧野英司
    • Journal Title

      精密工学会春季大会講演論文集

      Pages: 239-240

    • Related Report
      2006 Annual Research Report
  • [Journal Article] オンチップ細胞サージェリーシステムのためのマイクロニードルアレイの開発(第2報)-ニードルの機械的強度の評価-2007

    • Author(s)
      中西 彬, 酒井貴浩, 柴田隆行, 川島貴弘, 堀内 宰, 峯田 貴, 牧野英司
    • Journal Title

      精密工学会春季大会講演論文集

      Pages: 241-242

    • NAID

      130005028407

    • Related Report
      2006 Annual Research Report
  • [Journal Article] 細胞サージェリーのためのマイクロニードルアレイの開発2006

    • Author(s)
      柴田隆行, 吉田典央, 川島貴弘, 水上良明, 堀内 宰, 峯田 貴, 牧野英司
    • Journal Title

      精密工学会誌 72・12

      Pages: 1520-1524

    • NAID

      110004863752

    • Related Report
      2006 Annual Research Report
  • [Journal Article] オンチップ細胞サージェリーシステムのためのマイクロニードルアレイの開発2006

    • Author(s)
      中西 彬, 柴田隆行, 川島貴弘, 堀内 宰, 峯田 貴, 牧野英司
    • Journal Title

      表面技術協会第114回講演大会論文集

      Pages: 205-206

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Fabrication and fluid ejection performance of microneedle array for cellular function analysis

    • Author(s)
      T. Shibata, N. Kato, T. Sakai, T. Kawashima, M. Nomura, T. Mineta, E. Makino
    • Journal Title

      Proc. of the 3rd JSME/ASME International Conference on Materials and Processing (ICM&P 2008)(to bepublished)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Insertion performance of hollow microneedle array for cell surgery

    • Author(s)
      T. Kawashima, T. Shibata, T. Sakai, N. Kato, M. Nomura, T. Mineta, E. Makino
    • Journal Title

      Proc. of Asia-Pacific Conference on Transducers and Micro-Nano Technology 2008 (APCOT 2008) (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] オンチップ細胞サージェリーシステムのためのマイクロニードルアレイの開発(第3報)-液体注入および穿刺性能の評価-2008

    • Author(s)
      酒井 貴浩, 柴田 隆行, 川島 貴弘, 峯田 貴, 牧野 英司
    • Organizer
      2008年度精密工学会春季大会
    • Place of Presentation
      川崎
    • Year and Date
      2008-03-17
    • Related Report
      2007 Annual Research Report
  • [Presentation] 細胞機能解析のためのマイクロニードル搭載型AFMプローブの開発2008

    • Author(s)
      加藤 統久, 伊藤 雅仁, 柴田 隆行, 川島 貴弘, 峯田 貴, 牧野 英司
    • Organizer
      日本機械学会東海支部第57期講演会
    • Place of Presentation
      名古屋
    • Year and Date
      2008-03-11
    • Related Report
      2007 Annual Research Report
  • [Presentation] Fabrication and fluid ejection performance of microneedle array for cellular function analysis2008

    • Author(s)
      T. Shibata, N. Kato, T. Sakai, T. Kawashima, M. Nomura, T. Mineta, E. Makino
    • Organizer
      The 3rd JSME/ASME International Conference on Materials and Processing (ICM&P 2008) (to be presented)
    • Place of Presentation
      Evanston, IL, USA
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Insertion performance of hollow microneedle array for cell surgery2008

    • Author(s)
      T. Kawashima, T. Shibata, T. Sakai, N. Kato, M. Nomura, T. Mineta, E. Makino
    • Organizer
      Asia-Pacific Conference on Transducers and Micro-Nano Technology 2008 (APCOT 2008)(to be presented)
    • Place of Presentation
      Tainan, Taiwan
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Development of microneedle array for cell surgery in chip-based system (3rd Report) -Performance of fluid injection and needle insertion2008

    • Author(s)
      T. Sakai, T. Shibata, T. Kawashima, T. Mineta, E. Makino
    • Organizer
      The Japan Society for Precision Engineering (JSPE) Spring Conference
    • Place of Presentation
      Kanagawa, Japan
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Development of AFM probe integrated with microneedle for cellular function analysis2008

    • Author(s)
      N. Kato, M. Ito, T. Shibata, T. Kawashima, T. Mineta, E. Makino
    • Organizer
      The 57th Annual Meeting of The Japan Society of Mechanical Engineers (JSME) Tokai Branch
    • Place of Presentation
      Nagoya, Japan
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] A novel design of AFM probe integrated with hollow microneedle for cellular functionanalysis2007

    • Author(s)
      N. Kato, T. Shibata, T. Kawashima, T. Mineta, E. Makino
    • Organizer
      International Meeting on AFM in Life Sciences and Medicine(AFM BioMed Conference)
    • Place of Presentation
      Besancon, France
    • Year and Date
      2007-06-07
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] A novel design of AFM probe integrated with hollow microneedle for cellular function analysis2007

    • Author(s)
      N. Kato, T. Shibata, T. Kawashima, T. Mineta, E. Makino
    • Organizer
      International Meeting on AFM in Life Sciences and Medicine(AFMBioMedConference)
    • Place of Presentation
      Besangon,France
    • Year and Date
      2007-06-07
    • Related Report
      2007 Annual Research Report
  • [Presentation] Development of MEMS devices for cell surgery and cellulm function analysis on a chip-based system2007

    • Author(s)
      N. Kato, T. Shibata, T. Sakai, S. Yamanaka, T. Kawashima
    • Organizer
      Technical Meeting on Linear Drives, IEE Japan
    • Place of Presentation
      Tokyo, Japan
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Development of bioprobe untegrated with microneedle for cellular function analysis (2nd Report) - Fabrication of microneedle with sharpened tip by multiple etching processes2007

    • Author(s)
      N. Kato, T. Shibata, T. Kawashima, T. Mineta, E. Makino
    • Organizer
      The Japan Society for Precision Engineering (JSPE) Autumn Conference
    • Place of Presentation
      Asahikawa, Japan
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Fabrication and mechanical characterization of microneedle array for cell surgery2007

    • Author(s)
      T. Shibata, A. Nakanishi, T. Sakai, N. Kato, T. Kawashima, T. Mineta, E. Makino
    • Organizer
      The 14th International Conference on Solid-State Sensors, Actuators and Microsystems (Transducers '07)
    • Place of Presentation
      Lyon, France
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] A novel design of AFM probe integrated with hollow microneedle for cellular function analysis2007

    • Author(s)
      N. Kato, T. Shibata, T. Kawashima, T. Mineta, E. Makino
    • Organizer
      International Meeting on AFM in Life Sciences and Medicine (AFM BioMed Conference)
    • Place of Presentation
      Barcelona, Spain
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Development of bioprobe integrated with microneedle for cellular function analysis (1st Report)- Fabrication processes of shape needle tips and microchannels2007

    • Author(s)
      N. Kato, T. Shibata, T. Kawashima, O. Horiuchi, T. Mineta, E. Makino
    • Organizer
      The Japan Society for Precision Engineering (JSPE) Spring Conference
    • Place of Presentation
      Tokyo, Japan
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Development of microneedle array for cell surgery in chip-based system (2nd Report) -Evaluation of mechanical strength of needles2007

    • Author(s)
      A. Nakanishi, T. Sakai, T. Shibata, T. Kawashima, O. Horiuchi, T. Mineta, E. Makino
    • Organizer
      The Japan Society for Precision Engineering (JSPE) Spring Conference
    • Place of Presentation
      Tokyo, Japan
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Development of microneedle array for cell surgery in chip-based system2006

    • Author(s)
      A. Nakanishi, T. Shibata, T. Kawashima, O. Horiuchi, T. Mineta, E. Makino
    • Organizer
      The 114th Annual Conference of The Surface Finishing Society of Japan
    • Place of Presentation
      Sapporo, Japan
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Patent(Industrial Property Rights)] マイクロニードル搭載型バイオプローブ, およびマイクロニードル搭載型バイオプローブの作製方法2006

    • Inventor(s)
      柴田隆行
    • Industrial Property Rights Holder
      豊橋技術科学大学
    • Industrial Property Number
      2006-236086
    • Filing Date
      2006-08-31
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Patent(Industrial Property Rights)] マイクロニードル搭載型バイオプローブ,およびマイクロニードル搭載型バイオプローブの作製方法2006

    • Inventor(s)
      柴田隆行
    • Industrial Property Rights Holder
      国立大学法人豊橋技術科学大学
    • Industrial Property Number
      2006-236086
    • Filing Date
      2006-08-31
    • Related Report
      2006 Annual Research Report

URL: 

Published: 2006-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi