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Development of photoacoustic imaging with near-infrared pulse laser to improve depth resolution

Research Project

Project/Area Number 18300153
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Biomedical engineering/Biological material science
Research InstitutionKyoto Prefectural University of Medicine

Principal Investigator

TAKAMATSU Tetsuro  Kyoto Prefectural University of Medicine, Graduate School of Medical Science, Professor (40154900)

Co-Investigator(Kenkyū-buntansha) YAMAOKA Yoshiisa  Kyoto Prefectural University of Medicine, Graduate School of Medical Science, Assistant Professor (80405274)
Project Period (FY) 2006 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥16,810,000 (Direct Cost: ¥15,400,000、Indirect Cost: ¥1,410,000)
Fiscal Year 2007: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
Fiscal Year 2006: ¥10,700,000 (Direct Cost: ¥10,700,000)
KeywordsPhotoacoutic imaging / Bioimaging / Nonlinear optics / Multiphoton absorption / Infrared lights / Ultrafast light pulses / Medical and biological engineering / Vascular imaging
Research Abstract

Commercial imaging systems, such as computed tomography (CT) and magnetic resonance imaging (MRI), are frequently used powerful tools for observing structures deep within the human body. However, they cannot precisely visualized several-tens micrometer-sized structures for lack of spatial resolution. In this research, we proposed a multiphoton excitation-assisted photoacoustic tomography (MEAPAT) as a means of improving depth resolution. Since the multiphoton absorption occurs at only the focus point and the employed infrared pulses deeply penetrate living tissues, it enables us to extract characteristic features of structures embedded in the living tissue. When nanosecond pulses from a 1064-nm Nd:YAG laser were focused on Rhodamine B solution (absorption peak: 540 nm), the peak intensity of the generated photoacoustic signal detected by a 10 MHz transducer was proportional to the square of the input pulse energy. This result shows that the photoacoustic signals can be induced by the two-photon absorption of infrared nanosecond pulse laser and also can be detected by a commercial low-frequency MHz transducer. This means that MEAPAT overcomes the depth limitation of existing one-photon photoacoustic tomography caused by the need of a high-frequency transducer to improve depth resolution. Furthermore, in order to evaluate the depth resolution of MEAPAT, we compared the image of MEAPAT with that of one-photon photoacoustic imaging of a vascular phantom in a water bath. As a result, we found that the depth resolution of MEAPAT (1064 nm) is greater than that of one-photon photoacoustic imaging (532 nm).We conclude that evolving multiphoton-photoacoustic imaging technology renders feasible the investigation of biomedical phenomena at the deep layer in living tissue.

Report

(3 results)
  • 2007 Annual Research Report   Final Research Report Summary
  • 2006 Annual Research Report
  • Research Products

    (54 results)

All 2008 2007 2006

All Journal Article (30 results) (of which Peer Reviewed: 10 results) Presentation (20 results) Book (2 results) Patent(Industrial Property Rights) (2 results)

  • [Journal Article] Imaging of anticancer agent distribution by a sht-scanning Raman microscope2008

    • Author(s)
      Harada Y, Ota T, Dai P, Yamaoka Y, Hamada K, Fujita K, Takamatsu T
    • Journal Title

      Proceedings of SPIE 6853

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Mucosa llayer as major source of green autofluorescence in the colon under excitation by blue light2008

    • Author(s)
      Nakano K, Harada Y, Yamaoka Y, Miyawaki K, Wakabayasbi N, Mitsufuji S, Imaizumi K, Takaoka H, Nakaoka M, Takamatsu T
    • Journal Title

      Proceedings of SPIE 6853

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Generation of reentrant arrhythmias by dominant-nagative inhibition of connexin43 in rat cultured myoqyte monolayers2008

    • Author(s)
      Nakagami T, Tanaka H, Dai P, Tanabe T, Mani H, Fujiwara K, Lin SF, Matsubara H, Takamatsu T
    • Journal Title

      Cardiovascular Research (In press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Ar山ythmogenic substrates in myocardial infarct2008

    • Author(s)
      Takamatsu T
    • Journal Title

      Pathology International (In press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Imaging of anticancer agent distribution by a slit-scanning Raman microscope2008

    • Author(s)
      Harada, Y., Ota, T., Dai, P., Yamaoka, Y., Hamada, K., Fujita, K., Takamatsu, T
    • Journal Title

      Proceedings of SPIE 6853

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Mucosal layer as major source of green autofluorescence in the colon under excitation by blue light2008

    • Author(s)
      Nakano, K., Harada, Y., Yamaoka, Y., Miyawaki, K., Wakabayashi, N., Mitsufuji, S., Imaizumi, K., Takaoka, H., Nakaoka, M., Takamatsu, T
    • Journal Title

      Proceedings of SPIE 6853

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Generation of reentrant arrhythmias by dominant-nagative inhibition of connexin43 in rat cultured myocyte monolayers2008

    • Author(s)
      Nakagami, T., Tanaka, H., Dai, P., Tanabe, T., Math, H., Fujiwara, K., Lin, SF., Matsubara, H., Takamatsu, T
    • Journal Title

      Cardiovascular Research in press

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Arrythmogenic substrates in myocardial infarct2008

    • Author(s)
      Takamatsu, T
    • Journal Title

      Pathology International in press

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Functional attenuation of target proteins by multiphoton excitation-evoked chromophere-assisted laser inactivation (MP-CALI)2008

    • Author(s)
      Takamatsu, T
    • Journal Title

      Molecular imaging technology Medical Do 25-130

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Imaging of anticancer agent distribution by a slit-scanning Raman microscope2008

    • Author(s)
      Harada Y, Ota T, Dai P, Yamaoka Y, Hamada K, Fujita K, Takamatsu T
    • Journal Title

      Proceedings of SPIE 6853

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Mucosal layer as major source of green autofluorescence in the colon under excitation by blue light2008

    • Author(s)
      Nakano K, Harada Y, Yamaoka Y, Miyawaki K, Wakabayashi N, Mitsufuji S, Imaizumi K, Takaoka H, Nakaoka M, Takamatsu T
    • Journal Title

      Proceedings of SPIE 6853

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Generation of reentrant arrhythmias by dominant-nagative inhibition of connexin43 in rat cultured myocyte monolayers2008

    • Author(s)
      Nakagami T, Tanaka H, Dai P, Tanabe T, Mani H, Fujiwara K, Lin SF, Matsubara H, Takamatsu T
    • Journal Title

      Cardiovascular Research (in press)

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Improvement of depth resolution on photoacoustic imaging using multip hoton absorption2007

    • Author(s)
      Yamaoka Y, Fujiwara K, Takamatsu T
    • Journal Title

      Proceedings of SPIE 6631

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Attenuation of molecular function by multiphoton excitation-evoked chromophore-assisted laser inactivation (MP-CALI) using green fluorescent protein2007

    • Author(s)
      Takamatsu T
    • Journal Title

      Proceedings of SPIE 6460

      Pages: 646004-646011

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Improvement of depth resolution on photoacoustic imaging using multiphoton absorption2007

    • Author(s)
      Yamaoka, Y., Fujiwara, K., Takamatsu, T
    • Journal Title

      Proceedings of SPIE 6631

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Attenuation of molecular function by multiphoton excitation-evoked chromophore-assisted laser inactivation (MP-CALI) using green fluorescent protein2007

    • Author(s)
      Takamatsu, T
    • Journal Title

      Proceedings of SPIE 6460

      Pages: 646004-646011

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Basic and application of confocal laser scanning microscopy2007

    • Author(s)
      Harada, Y., Takamatsu, T
    • Journal Title

      Histochemistry and Cytochemistry 2007, Japan society of histochemistry and cytochemistry

      Pages: 25-33

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Improvement of depth resolution on photoacoustic imaging using multiphoton absorption2007

    • Author(s)
      Yamaoka Y, Fujiwara K, Takamatsu T
    • Journal Title

      Proceedings of SPIE 6631

    • Related Report
      2007 Annual Research Report
  • [Journal Article] Attenuation of molecular function by multiphoton excitation-evoked chromophore-assisted laser inactivation (MP-CALL) using green fluorescent protein2007

    • Author(s)
      Tetsuro Takamatsu
    • Journal Title

      Proceedings of SPIE 6460

      Pages: 646004-646011

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Location-dependent photogeneration of calcium waves in HeLa cells2006

    • Author(s)
      Iwanaga S, Kaneko T, Fujita K, Smith N, Nakamura O, Takamatsu T, Kawata S
    • Journal Title

      Cell Biochemistry and Biophysics 45・2

      Pages: 167-176

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] 多光子励起を利用した標的分子機能阻害法2006

    • Author(s)
      山岡 禎久、田邉 卓爾、高松 哲郎
    • Journal Title

      細胞工学 25

      Pages: 1032-1036

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] 非線形光学顕微鏡を用いたバイオイメージング2006

    • Author(s)
      高松 哲郎
    • Journal Title

      光技術ニンタクト 44

      Pages: 55-56

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] 光で生体の機能をより高い空間的・時間的分解能で捉える-2光子顕微鏡によるタンパク分子機能の計測・制御2006

    • Author(s)
      高松 哲郎
    • Journal Title

      Biophilia 2

      Pages: 20-23

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Location-dependent photogeneration of calcium waves in HeLa cells2006

    • Author(s)
      Iwanaga, S., Kaneko, T., Fujita, K., Smith, N., Nakamura, O., Takamatsu, T., Kawata, S
    • Journal Title

      Cell Biochemistry and Biophysics 45

      Pages: 167-176

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Multiphoton excitation-evoked chromophore-assisted laser inactivation of genetically-tagged proteins2006

    • Author(s)
      Yamaoka, Y., Tanabe, T., Takamatsu, T
    • Journal Title

      Cell Technology 25

      Pages: 1032-1036

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Bioimaging technology using nonlinear optical microscopy2006

    • Author(s)
      Takamatsu, T
    • Journal Title

      Optical and Electro-Optical Engineering Contant 44

      Pages: 55-56

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Measurement and control of protein functions with two-photon microscopy2006

    • Author(s)
      Takamatsu, T
    • Journal Title

      Biophilia 2

      Pages: 20-23

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Location-dependent photogeneration of calcium waves in HeLa cells2006

    • Author(s)
      Iwanaga S, Kaneko T, Fujita K, Smith N, Nakamura O, Takamatsu T, Kawata S
    • Journal Title

      Cell biochemistry and Biophysics 45・2

      Pages: 167-176

    • Related Report
      2006 Annual Research Report
  • [Journal Article] 多光子励起を利用した標的分子機能阻害法2006

    • Author(s)
      山岡禎久, 田邉卓爾, 高松哲郎
    • Journal Title

      細胞工学 25・9

      Pages: 1032-1036

    • Related Report
      2006 Annual Research Report
  • [Journal Article] 非線形光学顕微鏡を用いたバイオイメージング2006

    • Author(s)
      高松哲郎
    • Journal Title

      光技術コンタクト 44

      Pages: 55-56

    • Related Report
      2006 Annual Research Report
  • [Presentation] Mucosal layer as major source of green autofluorescence in the colon under excitation by blue light2008

    • Author(s)
      Nakano K, Harada Y, Yamaoka Y, Miyawaki K, Wakabayashi N, Mitsufuji S, Imaizumi K, Takaoka H, Nakaoka M, Takamatsu T
    • Organizer
      Photonics West 2008
    • Place of Presentation
      アメリカ、サンノゼ
    • Year and Date
      2008-01-23
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
  • [Presentation] Mucosal layer as major source of green autofluorescence in the colon under excitation by blue light2008

    • Author(s)
      Nakano, K., Harada, Y., Yamaoka, Y., Miyawaki, K., Wakabayashi, N., Mitsufuji, S., Imaizumi, K., Takaoka, H., Nakaoka, M., Takamatsu, T
    • Organizer
      Photonics West 2008
    • Place of Presentation
      San Jose
    • Year and Date
      2008-01-23
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Imaging of anticancer agent distribution by a sht-scanning Raman microscope2008

    • Author(s)
      Harada Y, Ota T, Dai P, Yamaoka Y, Hamada K, Fujita K, Takamatsu T
    • Organizer
      Photonics West 2008
    • Place of Presentation
      アメリカ、サンノゼ
    • Year and Date
      2008-01-19
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Imaging of anticancer agent distribution by a slit-scanning Raman microscope2008

    • Author(s)
      Harada, Y., Ota, T., Dai, P., Yamaoka, Y., Hamada, K., Fujita, K., Takamatsu, T
    • Organizer
      Photonics West 2008
    • Place of Presentation
      San Jose
    • Year and Date
      2008-01-19
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Imaging of anticancer agent distribution by a slit-scanning Raman microscope2008

    • Author(s)
      Harada Y, Ota T, Dai P, Yamaoka Y, Hamada K, Fujita K, Takamatsu T
    • Organizer
      Photonics West 2008
    • Place of Presentation
      アメリカ、サンノゼ
    • Year and Date
      2008-01-19
    • Related Report
      2007 Annual Research Report
  • [Presentation] Cx43 as a positive regulator of TGF-β/Smad signaling2007

    • Author(s)
      Dai P, Nakagami T, Tanaka H, Hitomi T, Takamatsu T
    • Organizer
      47th annual meeting of American Society of Cell Biology
    • Place of Presentation
      アメリカ、ワシントンDC
    • Year and Date
      2007-12-03
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
  • [Presentation] Cx43 as a positive regulator of TGF-13/Smad signaling2007

    • Author(s)
      Dai, P., Nakagami, T., Tanaka, H., Hitomi, T., Takamatsu, T
    • Organizer
      47th annual meeting of American Society of Cell Biology
    • Place of Presentation
      Washington DC
    • Year and Date
      2007-12-03
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 非線形光学現象を利用た光音響断層撮影装置の開発2007

    • Author(s)
      山岡 禎久
    • Organizer
      京都産学公連携フォーラム2007
    • Place of Presentation
      京都市
    • Year and Date
      2007-11-01
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 非線形光学現象を利用した光音響断層撮影装置の開発2007

    • Author(s)
      山岡禎久
    • Organizer
      京都産学公連携フォーラム2007
    • Place of Presentation
      京都市
    • Year and Date
      2007-11-01
    • Related Report
      2007 Annual Research Report
  • [Presentation] Improvement of depth resoluhon on photoacoustic imaging using multip hoton absorption2007

    • Author(s)
      Yamaoka Y, Fujiwara K, Takamatsu T
    • Organizer
      European Conference on Biomedical Optics 2007
    • Place of Presentation
      ドイツ、ミュンヘン
    • Year and Date
      2007-06-17
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Improvement of depth resolution on photoacoustic imaging using multiphoton absorotion2007

    • Author(s)
      Yamaoka, Y., Fujiwara, K., Takamatsu, T
    • Organizer
      European Conference on Biomedical Optics
    • Place of Presentation
      Munich
    • Year and Date
      2007-06-17
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Improvement of depth resolution on photoacoustic imaging using multiphoton absorption2007

    • Author(s)
      Yamaoka Y, Fujiwara K, Takamatsu T
    • Organizer
      European Conference on Biomedical Optics 2007
    • Place of Presentation
      ドイツ、ミュンヘン
    • Year and Date
      2007-06-17
    • Related Report
      2007 Annual Research Report
  • [Presentation] 生体深部観察のための多光子励起光音響イメージングの提案2007

    • Author(s)
      山岡 禎久、藤原 克次、高松 哲郎
    • Organizer
      第33回レーザ顕微鏡研究会
    • Place of Presentation
      和光市
    • Year and Date
      2007-05-27
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
  • [Presentation] Photoacoustic imaging using multiphoton absorption in deep biological tissues2007

    • Author(s)
      Yamaoka, Y., Takamatsu, T
    • Organizer
      The 46th Annual Conference of Japanese Society for Medical and Biological Engineering
    • Place of Presentation
      Sendai
    • Year and Date
      2007-05-27
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Proposal for multiphoton excitation-evoked photoacoustic imaging for observation of deep structures in living tissues2007

    • Author(s)
      Yamaoka, Y., Fujiwara, K., Takamatsu, T
    • Organizer
      SLM 33
    • Place of Presentation
      Wako
    • Year and Date
      2007-05-27
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 多光子励起光音響波を利用した生体深部イメージングの検討2007

    • Author(s)
      山岡 禎久、高松 哲郎
    • Organizer
      第46回日本生体医工学会大会
    • Place of Presentation
      仙台市
    • Year and Date
      2007-04-25
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
  • [Presentation] Difference of photoacoustic waves between various tissues2006

    • Author(s)
      Yamaoka Y, Takamatsu T
    • Organizer
      7th Joint Meeting of the Histochemical Society and the Japan Society of Histochemistry and Cytochemistry
    • Place of Presentation
      アメリカ、ハワイ
    • Year and Date
      2006-08-24
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] NIR laser-based manipulation of functional molecules in living cells2006

    • Author(s)
      Yamaoka Y, Takamatsu T
    • Organizer
      7th Joint Meeting of the Histochemical Society and the Japan Society of Histochemistry and Cyto chemistry
    • Place of Presentation
      アメリカ、ハワイ
    • Year and Date
      2006-08-24
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Difference of photoacoustic waves between various tissues2006

    • Author(s)
      Yamaoka, Y., Takamatsu, T
    • Organizer
      7th Joint Meeting of the Histochemical Society and the Japan Society of Histochemistry and Cytochemistry
    • Place of Presentation
      Hawaii
    • Year and Date
      2006-08-24
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] NIR laser based manipulation of functional molecules in living cells2006

    • Author(s)
      Yamaoka, Y., Takamatsu, T
    • Organizer
      7th Joint Meeting of the Histochemical Society and the Japan Society of Histochemistry and Cytochemistry
    • Place of Presentation
      Hawaii
    • Year and Date
      2006-08-24
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Book] ますます広がる分子イメージング技術、第1章、3-42008

    • Author(s)
      高松 哲郎(執筆分担)
    • Total Pages
      5
    • Publisher
      株式会社メディカルドゥ
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Book] 組織細胞化学2007、コンフォーカル顕微鏡の基礎と応用2007

    • Author(s)
      原田 義規、高松 哲郎(執筆分担)
    • Total Pages
      9
    • Publisher
      日本組織細胞化学会
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Patent(Industrial Property Rights)] 光音響断層撮影装置及び光音響断層撮影方法2007

    • Inventor(s)
      高松哲郎山岡禎久
    • Industrial Property Rights Holder
      関西TLO
    • Filing Date
      2007-01-31
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Patent(Industrial Property Rights)] 光音響断層撮影装置及び光音響断層撮影方法2007

    • Inventor(s)
      高松哲郎, 山岡禎久
    • Industrial Property Rights Holder
      関西TLO
    • Filing Date
      2007-01-31
    • Related Report
      2006 Annual Research Report

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Published: 2006-04-01   Modified: 2016-04-21  

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