| Project/Area Number |
18300228
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied health science
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| Research Institution | Chiba University |
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Principal Investigator |
TAKIGUCHI Masaki Chiba University, 大学院・医学研究院, 教授 (40179578)
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| Co-Investigator(Kenkyū-buntansha) |
HIWASA Takaki 千葉大学, 大学院・医学研究院, 准教授 (30260251)
TAMURA Yutaka 千葉大学, 大学院・医学研究院, 准教授 (50263174)
IWASE Katuro 千葉大学, 大学院・医学研究院, 講師 (80322030)
MATUMOTO Eriko 千葉大学, 大学院・医学研究院, 技術職員 (20422256)
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| Project Period (FY) |
2006 – 2009
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| Project Status |
Completed (Fiscal Year 2009)
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| Budget Amount *help |
¥18,460,000 (Direct Cost: ¥15,400,000、Indirect Cost: ¥3,060,000)
Fiscal Year 2009: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2008: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2007: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2006: ¥5,200,000 (Direct Cost: ¥5,200,000)
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| Keywords | 生活習慣病 / 概日リズム / 生物時計 / 日周リズム / 光応答性 / 尿素合成 / オルニチンサイクル / セクレトグラニンII / 遺伝子標的破壊マウス / SREBP / 脂質合成 / 栄養素 / 栄養組成 / 視交叉上核 / マイクロアレイ解析 / 酸化的リン酸化 / プロテアソーム / 光同調性時計 / 摂食同調性時計 |
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| Research Abstract |
Expression of the gene for sterol regulatory element-binding protein-1 (SREBP-1), a transcription factor pivotal in lipid biosynthesis, achieved a peak in the dark feeding period of the daily light/dark cycle in the liver of mice fed with a standard diet, and a peak in the light fasting period with high-carbohydrate, high-fat, and high-protein diets, underlining the importance of nutrients in shaping the daily rhythm. Expression of genes for the ornithine cycle, an enzyme system for ammonia detoxification, achieved a peak in the feeding period with a high-protein diet, and a peak in the late fasting to early feeding periods with a low-protein (high-carbohydrate) diet, suggesting activation of the expression both in the feeding and fasting periods because of requirement for detoxifying ammonia derived form amino acid degradation.
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