Identification and monitoring of key phylogenetic groups of marinebacteria involved in the emission of climate related gases

• Project/Area Number: 18310011
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Environmental dynamic analysis
• Research Institution: The University of Tokyo
• Principal Investigator: HAMASAKI Koji The University of Tokyo, 海洋研究所, 准教授 (80277871)
• Co-Investigator(Kenkyū-buntansha): NAGATA Toshi 東京大学, 海洋研究所, 教授 (40183892)
• Project Period (FY): 2006 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥17,660,000 (Direct Cost: ¥15,800,000、Indirect Cost: ¥1,860,000); Fiscal Year 2008: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000); Fiscal Year 2007: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2006: ¥9,600,000 (Direct Cost: ¥9,600,000)
• Keywords: 海洋 / 微生物 / 炭素循環 / ジメチルサルファイド / 海洋微生物 / 気候関連ガス / rRNA / プロモデオキシウリジン / 16SrRNA遺伝子 / 物質循環 / DMS / ブロモデオキシウリジン

Research Abstract

本研究では、海洋微生物の生態を解析するための新しい手法を開発し、これらを駆使して、海洋における炭酸ガスやジメチルサルファイド(DMS)といった気候関連ガスの動態に強い影響を及ぼす微生物鍵種を特定した。さらに、独自技術に次世代超並列シーケンスを組み合わせた解析では、従来法では不可能であった希少種も含めた増殖群の特定に成功し、微生物多様性と生態系の安定性を議論する上での重要な知見を得ることが出来た。

Research Products

• [Journal Article] Community structures of actively growing bacteria shift along a north-south transect in the western North Pacific (2008)
  • Author(s): Taniguchi, A., Hamasaki, K.
  • Journal Title: Environ Microb. 10 Pages: 1007-1017
  • Peer Reviewed
• [Journal Article] Community structures of actively growing bacteria shift along a north-south transect in the western North Pacific (2008)
  • Author(s): Taniguchi, A and Hamasaki, K
  • Journal Title: Environmental Microbiology 10 Pages: 1007-1017
  • Peer Reviewed
• [Journal Article] Communlty structures of actively growing bacteria shift along a north-south transect in the western North Pacific (2008)
  • Author(s): Taniguchi, A
  • Journal Title: Environ Microb 10 Pages: 1007-1017
  • Peer Reviewed
• [Journal Article] 海洋における光合成細菌の分布と微生物海洋学への展望 (2007)
  • Author(s): 浜崎恒二
  • Journal Title: 日本微生物生態学会誌 22 Pages: 5-14
  • NAID: 110006272184
• [Journal Article] Actively growing bacteria in the Inland Sea of Japan identified by combined bromodeoxyuridine immunocapture and denaturing gradient gel electrophoresis (2007)
  • Author(s): Hamasaki, K., Taniguchi, A., Tada, Y., Long, RA, Azam, F.
  • Journal Title: Appl. Environ. Microbiol 73 Pages: 2787-2798
  • Peer Reviewed
• [Journal Article] Size distribution and biomass of nanoflagellates in meso-and bathypelagic layers of the subarctic Pacific (2007)
  • Author(s): Fukuda, H., Sohrin, R., Nagata, T., Koike, I.
  • Journal Title: Aquatic Microbial Ecology 46 Pages: 203-207
  • Peer Reviewed
• [Journal Article] Enhancement of viral production by addition of nitrogen or nitrogen plus carbon in subtropical surface waters of the South Pacific (2007)
  • Author(s): Motegi, C., Nagata, T.
  • Journal Title: Aquatic Microbial Ecology 48 Pages: 27-34
  • Peer Reviewed
• [Journal Article] Actively growing bacteria in the Inland Sea of Japan identified by combined bromodeoxyuridine immunocapture and denaturing gradient gel electrophoresis (2007)
  • Author(s): Hamasaki, K
  • Journal Title: Appl.Environ.Microbiol 73 Pages: 2787-2798
  • Peer Reviewed
• [Journal Article] 海洋における光合成細菌の分布と微生物海洋学への展望 (2007)
  • Author(s): 浜崎恒二
  • Journal Title: 日本微生物生態学会誌 22・1 Pages: 5-14
  • NAID: 110006272184
• [Journal Article] Comparison of bromodeoxyuridine immunoassay with tritiated thymidine radioassay for measuring bacterial productivity in oceanic waters (2006)
  • Author(s): Hamasaki, K
  • Journal Title: J. Oceanogr. J. Oceanogr 62 Pages: 793-799
  • NAID: 10018405641
  • Peer Reviewed
• [Journal Article] Comparison of bromodeoxyuridine immunoassay with tritiated thymidine radioassay for measuring bacterial productivity in oceanic waters. (2006)
  • Author(s): Hamasaki, K
  • Journal Title: J. Oceanography 62 Pages: 793-799
  • NAID: 10018405641
• [Journal Article] 細菌群集の現存量および群集組成 (2006)
  • Author(s): 浜崎恒二
  • Journal Title: 海洋生物の連鎖-生命は他の生命および環境とどのように連携しているか (海洋生命系のダイナミクス・シリーズ3) Pages: 103-126
• [Journal Article] Actively growing bacteria in the Inland Sea of Japan identified by combined bromodeoxyuridine immunocapture and denaturing gradient gel electrophoresis
  • Author(s): Hamasaki, K., Taniguchi, A., Tada, Y., Long, RA, Azam, F.
  • Journal Title: Appl. Environ. Microbiol. In press
• [Presentation] Massively Parallel Sequencing of 16S rRNA V6 Regions Revealed the Diversity of Active-but-Rare Microbial Populations in Oceanic Pelagic Ecosystems (2009)
  • Author(s): Hamasaki, K, Taniguchi, A
  • Organizer: 1st Korea-Japan Symposium on Microbial Ecology, Jeju
  • Year and Date: 2009-05-29
• [Presentation] Diversity of active microbial communities in the South Pacific Ocean revealed by bromodeoxyuridine labeling and 454 tag sequencing technology (2009)
  • Author(s): Hamasaki, K, Taniguchi, A
  • Organizer: DNA Barcoding of Marine Biodiversity (MarBOL)
  • Place of Presentation: Tokyo
  • Year and Date: 2009-05-21
• [Presentation] Drastic change of the biomass and productivity of major subgroups of bacteria along a steep environmental gradient in the Southern Ocean frontal systems (2009)
  • Author(s): Tada, Y, Taniguchi A, Hamasaki, K
  • Organizer: SCAR Biology Symposium, Sapporo
• [Presentation] Identification of actively growing bacteria in seawater supplemented with dimethylsulfoniopropionate (DMSP) in the Southern Ocean (2009)
  • Author(s): Okamoto, A, Tada, Y, Kasamatsu, N, Hamasaki, K
  • Organizer: SCAR Biology Symposium, Sapporo
• [Presentation] Diversity of active microbial communities in surface seawaters along a north-south transect in the South Pacific Ocean (2009)
  • Author(s): Hamasaki, K, Taniguchi, A
  • Organizer: International Census of Marine Microbes 454 Users Spring Meeting, Woods Hole
• [Presentation] Distribution and abundance of aerobic anoxygenic phototrophic bacteria in temperate neritic water of the Otsuchi Bay, northern Japan (2009)
  • Author(s): Sato, Y, Fukuda, H, Hamasaki, K
  • Organizer: ASLO aquatic science meeting
  • Place of Presentation: Niece
• [Presentation] 西部北太平洋植物プランクトンブルームにおける細菌群集の動態解析 (2009)
  • Author(s): 多田雄哉, 谷口亮人, 津田敦, 植松光夫, 浜崎恒二
  • Organizer: 日本海洋学会
  • Place of Presentation: 2009
• [Presentation] Bacterial community structures and luminescence of the fecal pellets of Antarctic krill and salps (2008)
  • Author(s): Nishikawa, J, Tada, Y, Wada, M, Hamasaki, K.
  • Organizer: XXXI Symposium on Polar Biology
  • Place of Presentation: Tokyo
  • Year and Date: 2008-12-05
• [Presentation] Bacterial community structures and luminescence of the fecal pellets of Antarctic krill and salps (2008)
  • Author(s): J. Nishikawa, Y. Tada, M. Wada, K. Hamasaki
  • Organizer: XXXI Symposium on Polar Biology
  • Place of Presentation: 東京
  • Year and Date: 2008-12-05
• [Presentation] 酉部北太平洋における植物プランクトンブルーム内外の細菌群集構造 (2008)
  • Author(s): 多田 雄哉
  • Organizer: 日本海洋学会春季大会
  • Place of Presentation: 東京海洋大学
  • Year and Date: 2008-03-28
• [Presentation] ボトムアップ効果による海洋細菌群集構造の通年変化 (2008)
  • Author(s): 谷口 亮人
  • Organizer: 日本海洋学会春季大会
  • Place of Presentation: 東京海洋大学
  • Year and Date: 2008-03-28
• [Presentation] 南太平洋における細菌亜集団の増殖活性 (2008)
  • Author(s): 神谷 英里子
  • Organizer: 日本海洋学会春季大会
  • Place of Presentation: 東京海洋大学
  • Year and Date: 2008-03-28
• [Presentation] 相模湾西部沿岸域における従属栄養細菌の時空間変動解析一特にその生産力と群集構造 (2008)
  • Author(s): 寺西 聡子
  • Organizer: 日本海洋学会春季大会
  • Place of Presentation: 東京海洋大学
  • Year and Date: 2008-03-28
• [Presentation] Bacterial colonization and luminescence on fecal pellets of macrozooplankton (2008)
  • Author(s): Tada, Y, Wada, M, Nishikawa, J, Hamasaki, K.
  • Organizer: 12th International Symposium on Microbial Ecology, Cairns
• [Presentation] 中栄養沿岸域における酸素非発生型好気性光合成細菌の現存量の変動 (2008)
  • Author(s): 佐藤由季, 福田秀樹, 谷口亮人, 吉田典明, 浜崎恒二
  • Organizer: 日本微生物生態学会
  • Place of Presentation: 札幌
• [Presentation] サロマ湖海氷細菌群集構造解析-極域海氷細菌群はサロマ湖海氷で生きていけるか- (2008)
  • Author(s): 谷口亮人, 多田雄哉, 浜崎恒二
  • Organizer: 日本海洋学会
  • Place of Presentation: 広島
• [Presentation] 植物プランクトンブルーム崩壊過程における細菌群集の動態解析 (2008)
  • Author(s): 多田雄哉, 谷口亮人, 吉田典明, 浜崎恒二
  • Organizer: 日本海洋学会
  • Place of Presentation: 広島
• [Presentation] 西部北太平洋における植物プランクトンブルーム内外の細菌群集構造 (2008)
  • Author(s): 多田雄哉, 谷口亮人, 津田敦, 植松光夫, 浜崎恒二
  • Organizer: 日本海洋学会
  • Place of Presentation: 東京
• [Presentation] 海洋細菌によるサブミクロン粒子捕獲量と分解酵素活性 (2008)
  • Author(s): 菅友美, 大林由美子, 池本栄子, 菊池知彦, 浜崎恒二, 木暮一啓
  • Organizer: 日本海洋学会
  • Place of Presentation: 東京
• [Presentation] 相模湾西部沿岸域における従属栄養性細菌の時空間変動解析 特にその生産力と群集構造について (2008)
  • Author(s): 寺西聡子, 谷口亮人, 浜崎恒二, 菊池知彦
  • Organizer: 日本海洋学会
  • Place of Presentation: 東京
• [Presentation] サロマ湖海氷細菌群集構造解析-極域海氷細菌群はサロマ湖海氷で生きていけるか- (2008)
  • Author(s): 谷ロ亮人・多田雄哉・浜崎恒二
  • Organizer: 日本海洋学会秋季大会
  • Place of Presentation: 広島
• [Presentation] 植物プランクトンブルーム崩壊過程における細菌群集の動態解析 (2008)
  • Author(s): 多田雄哉・谷ロ亮人・吉田典明・浜崎恒二
  • Organizer: 日本海洋学会秋季大会
  • Place of Presentation: 広島
• [Presentation] Bacterial colonization and luminescence on fecal pellets of macrozooplankton (2008)
  • Author(s): Y. Tada, M. Wada, J. Nishikawa, K. Hamasaki
  • Organizer: 12th International Symposium on Microbial Ecology
  • Place of Presentation: Cairns (Australia)
• [Presentation] 大規模南北トランゼクトの細菌群集構造の空間的変動 (2007)
  • Author(s): 谷口 亮人
  • Organizer: 日本海洋学会秋季大会
  • Place of Presentation: 琉球大学工学部
  • Year and Date: 2007-09-26
• [Presentation] サルパ糞粒中の細菌叢の解析 (2007)
  • Author(s): 浜崎 恒二
  • Organizer: 日本海洋学会秋季大会
  • Place of Presentation: 琉球大学工学部
  • Year and Date: 2007-09-26
• [Presentation] 大規模南北トランゼクトにおける細菌群集構造の空間的変動 (2007)
  • Author(s): 谷口亮人, 多田雄哉, 横川太一, 茂手木千晶, ヤン・ヤンヒュイ, 永田俊, 浜崎恒二
  • Organizer: 日本海洋学会
  • Place of Presentation: 沖縄
• [Presentation] 海洋中のサブミクロン粒子を捕獲する細菌の分布と現存量 (2007)
  • Author(s): 菅友美, 徐維那, 池本栄子, 谷口亮人, 多田雄哉, 寺西聡子, 浜崎恒二, 菊池知彦, 木暮一啓
  • Organizer: 日本海洋学会
  • Place of Presentation: 沖縄
• [Presentation] サルパ類糞粒中の微生物叢の解析 (2007)
  • Author(s): 浜崎恒二, 多田雄哉, 和田実, 西川淳
  • Organizer: 日本海洋学会
  • Place of Presentation: 沖縄
• [Presentation] 岩手県大槌湾における酸素非発生型好気性光合成細菌の分離と現存量の推定 (2007)
  • Author(s): 佐藤由季, 浜崎恒二
  • Organizer: 日本海洋学会
  • Place of Presentation: 沖縄
• [Presentation] マイクロビーズアレイシステムを用いた海洋細菌の群集構造解析 (2007)
  • Author(s): 多田雄哉, 谷口亮人, 浜崎恒二
  • Organizer: 日本微生物生態学会
  • Place of Presentation: 愛媛
• [Presentation] Bacterial growth response in seawater environments : application of halogenated nucleoside, bromodeoxyuridine incorporation (2006)
  • Author(s): Hamasaki, K
  • Organizer: 2^<nd> KIOS-ORI Joint Symposium on Marine Science, Pusan
• [Presentation] Monitoring key phylogenetic groups responsible for bacterial production in coastal seawater (2006)
  • Author(s): Hamasaki, K, Tada, Y, Taniguchi, A
  • Organizer: 11^<th> International Symposium on Microbial Ecology
  • Place of Presentation: Vienna
• [Presentation] Determining key species responsible for bacterial production in coastal and oceanic waters (2006)
  • Author(s): Taniguchi, A, Hamasaki, K
  • Organizer: 11^<th> International Symposium on Microbial Ecology, Vienna
• [Presentation] 植物プランクトンブルームの形成・崩壊過程における細菌群集の変動-BUMP-DGGE法による解析- (2006)
  • Author(s): 谷口亮人, 多田雄哉, 浜崎恒二, 横川太一, 茂手木千晶, 永田俊
  • Organizer: 日本微生物生態学会
  • Place of Presentation: 東京
• [Presentation] 多田雄哉, 広島湾において活発に増殖する細菌系統群の季節変動谷口亮人, 浜崎恒二 (2006)
  • Author(s): 多田雄哉, 谷口亮人, 浜崎恒二
  • Organizer: 日本微生物生態学会
  • Place of Presentation: 東京