Study of Genome Stability of Germ Cells Using Medaka Mutants

• Project/Area Number: 18310038
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Risk sciences of radiation/Chemicals
• Research Institution: The University of Tokyo
• Principal Investigator: MITANI Hiroshi The University of Tokyo, 大学院・新領域創成科学研究科, 教授 (70181922)
• Co-Investigator(Kenkyū-buntansha): ODA Shoji 東京大学, 大学院・新領域創成科学研究科, 講師 (50266714) ; ISHIKAWA Tomoko 大阪大学, 大学院・医学系研究科, 助教 (70402922)
• Project Period (FY): 2006 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥17,820,000 (Direct Cost: ¥15,300,000、Indirect Cost: ¥2,520,000); Fiscal Year 2008: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2007: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000); Fiscal Year 2006: ¥6,900,000 (Direct Cost: ¥6,900,000)
• Keywords: 放射線 / 動物 / 遺伝学 / ストレス / 遺伝子 / 細胞・組織 / メダカ / 突然変異 / 魚類

Research Abstract

放射線高感受性ric1細胞は、DNA2本鎖切断の修復が遅延しており、γ線による断片化を伴う細胞死が起きない。緑色蛍光蛋白質(GFP)遺伝子を生殖細胞特異的に発現するric1系統を用いたところ、卵割期にすでにric1生殖細胞は、放射線高感受性であり、生殖腺移行後に孵化率に影響を及ぼさない線量に対して大きな性差があることも分かった。また、酸化損傷修復に関わるOGG1遺伝子の突然変異ホモ接合体メダカを作製した。

Research Products

• [Journal Article] The cytotoxicity and genotoxicity of hexavalent chromium in medaka(Oryzias latipes) (2008)
  • Author(s): Goodale, B.C., R. Walter, S.R. Pelsue, W.D. Thompson, S.S. Wise, R.N. Winn, H. Mitani, J.P. Wise, Sr.
  • Journal Title: Aquat Toxicol 87 Pages: 60-67
• [Journal Article] CYR61 is a novel gene associated with temperature-dependent changes in fish metabolism as revealed by cDNA microarray analysis on a medaka Oryzias latipes cell line (2008)
  • Author(s): Hirayama, M., M.N. Ahsan, H. Mitani, S. Watabe.
  • Journal Title: J Cell Biochem 104 Pages: 1297-1310
• [Journal Article] DNA damage-induced ubiquitylation of RFC2 subunit of replication factor C complex (2008)
  • Author(s): Tomida J, Y.Masuda, H.Hiroaki, T.Ishikawa, I.Song , T.Tsurimoto, S.Tateishi, T. Shiomi, Y. Kamei, J. Kim, K. Kamiya, C. Vaziri, H. Ohmori, T. Todo
  • Journal Title: J Biol Chem 283 Pages: 9071-9079
• [Journal Article] Development of a convenient in vitro fertilization method using interspecific hybrids between Oryzias latipes and Oryzias curvinotus (2008)
  • Author(s): Kamei Y, J.Itou, S. Oda, M. Masui, J.H.Kim, T. Ishikawa, S. Yuba, M. Kinoshita, H.Mitani, T. Todo
  • Journal Title: J Biol Chem 283 Pages: 9071-9079
  • NAID: 10019984185
• [Journal Article] The cytotoxicity and genotoxicity of hexavalent chromium in medaka (Oryzias latipes) cells. (2008)
  • Author(s): Goodale, B. C
  • Journal Title: Aquat Toxicol 87 Pages: 60-67
  • Peer Reviewed
• [Journal Article] CYR61 is a novel gene associated with temperature-dependent changes in fish metabolism as revealed by cDNA microarray analysis on a medaka Oryzias latipes cell line. (2008)
  • Author(s): Hirayama, M.
  • Journal Title: J Cell Biochem 104 Pages: 1297-1310
  • Peer Reviewed
• [Journal Article] DNA damage-induced ubiquitylation of RFC2 subunit of replication factor C complex. (2008)
  • Author(s): Tomida, J.
  • Journal Title: J Biol Chem. 283 Pages: 9071-9079
  • Peer Reviewed
• [Journal Article] Development of a convenient in vitro fertilization method using interspecific hybrids between Oryzias latipes and Oryzias curvinotus (2007)
  • Author(s): Kamei Y, J.Itou, S. Oda, M. Masui, J.H.Kim, T. Ishikawa, S. Yuba, M. Kinoshita, H.Mitani, T. Todo
  • Journal Title: Dev Growth Differ 49 Pages: 721-730
  • NAID: 10019984185
• [Journal Article] Radiation hybrid maps of Medaka chromosomes LG 12, 17, and 21 (2007)
  • Author(s): Su, F., et. al.
  • Journal Title: DNA Res 14 Pages: 135-140
  • Peer Reviewed
• [Journal Article] Deficiency in the response to DNA double-strand breaks inmouse early preimplantation embryos (2007)
  • Author(s): Yukawa, M., et. al.
  • Journal Title: Biochem Biophys Res Commun 358 Pages: 578-584
  • Peer Reviewed
• [Journal Article] Responses of embryonic germ cells of the radiation-sensitive Medaka mutant to γ-irradiation. (2007)
  • Author(s): Aizawa, K. 他
  • Journal Title: J. Radat. Res. 48・2(in press)
• [Journal Article] Generation of medaka gene knockout models by target-selected mutagenesis (2006)
  • Author(s): Taniguchi, Y., Takeda, S., Furutani-Seiki, M., Kamei, Y., Todo, T., Sasado, T., Deguchi, T., Kondoh, H., Mudde, J., Yamazoe, M., Hidaka, M., Mitani, H., Toyoda, A., Sakaki, Y., Plasterk, R. H., Cuppen, E.
  • Journal Title: Genome Biol. 7 Pages: 112-112
• [Journal Article] Expression profiles of 4-nonylphenol-exposed medaka (O ryzias latipes) analyzed with a 3.4K microarray (2006)
  • Author(s): Kim, I. C., Lee, Y. M., Lee, C., Kim, H. M., Oda, S., Lee, Y. S., Mitani, H., Lee, J. S.
  • Journal Title: Mar Environ Res. 62Suppl1 Pages: 141-146
• [Journal Article] The DNA sequence of medaka chromosome LG22 (2006)
  • Author(s): Sasaki, T., Shimizu, A., Ishikawa, S. K.,Imai, S.,Asakawa, S.,Murayama,Y., Khorasani, M. Z., Mitani, H., Furutani-Seiki, M., Kondoh, H., Nanda, I., Schmid, M., Schartl, M., Nonaka, M., Takeda, H.,Hori, H., Himmelbauer, H., Shima, A., Shimizu, N.
  • Journal Title: Genomics 89 Pages: 124-133
• [Journal Article] The DNA sequence of medaka chromosome LG22. (2006)
  • Author(s): Sasaki, T., 他
  • Journal Title: Genomics 89・1 Pages: 124-133
• [Journal Article] Generation of medaka gene knockout models by target-selected mutagenesis. (2006)
  • Author(s): Taniguchi, Y., 他
  • Journal Title: Genome Biol. 7・12 Pages: 112-112
• [Presentation] DNA損傷応答遺伝子ric1の機能解析. (2008)
  • Author(s): 日高征幸
  • Organizer: 日本放射線影響学会
  • Place of Presentation: 北九州市
  • Year and Date: 2008-11-20
• [Presentation] Study of Genome Surveillance Mechanisms in Fish using Radiation Sensitive Medaka Mutant with defective repair of DNA double-strand breaks (2008)
  • Author(s): Mitani, H.
  • Organizer: The 4th International Aquatic Animal Models of Human Disease Conference
  • Place of Presentation: ダーラム米国
  • Year and Date: 2008-01-31
• [Presentation] Study of Genome Surveillance Mechanisms in Fish using Radiation Sensitive Medaka Mutant with Defective Repair of DNA Double-Strand Breaks (2008)
  • Author(s): Mitani H., Hidaka M., Yori K., Yamashita T., Nishino K., Urushihara U., Oda S.
  • Organizer: The 4th International Aquatic Animal Models of Human Disease Conference(AAMHD)
  • Place of Presentation: Duke University, Durham NC
• [Presentation] DNA損傷応答遺伝子ric1の機能解析 (2008)
  • Author(s): 日高征幸, 尾田正二, 漆原佑介, 桑原義和, 福本学, 三谷啓志
  • Organizer: 日本放射線影響学会第51回
  • Place of Presentation: 北九州
• [Presentation] メダカを用いたDNA二本鎖切断修復可視化 (2008)
  • Author(s): 漆原佑介, 尾田正二, 野田朝男, 小林純也, 小松賢志, 三谷啓志
  • Organizer: 日本放射線影響学会第51回
  • Place of Presentation: 北九州
• [Presentation] Highly radiosensitive germ cells in Medaka ricl mutant with abnormal DNA double-strand break reair and otosis induction (2007)
  • Author(s): Aizawa, K., et. al.
  • Organizer: The 13th Intemational Congress of Radiation Research
  • Place of Presentation: サンフランシスコ米国
  • Year and Date: 2007-07-09
• [Presentation] The mutation of ricl induces delayed repair of DNA double strand breaks,cell death inhibition and ear checint release (2007)
  • Author(s): Hidaka, M., et. al.
  • Organizer: The 13th Intemational Congress of Radiation Research
  • Place of Presentation: サンフランシスコ米国
  • Year and Date: 2007-07-09
• [Presentation] Highly radiosensitive germ cells in Medaka ric1 mutant with abnormal DNA double-strand break repair and apoptosis induction (2007)
  • Author(s): Mitani H., Aizawa K., Yori K., Kaminaga C., Yashita T., Nishino K., Hidaka M., Kinoshita M., Oda S.
  • Organizer: 13th International Congress of Radiation Research
  • Place of Presentation: San Francisco
• [Presentation] The mutation of ric1 induces delayed repair of DNA double strand breaks, cell death inhibition and early checkpoint release (2007)
  • Author(s): Hidaka M., Oda S., Kuwahara Y., Fukumoto M., Mitani H.
  • Organizer: 13th International Congress of Radiation Research
  • Place of Presentation: San Francisco
• [Presentation] High radiosensitivity and abnormal apoptosis in germ cells of medaka ric1 mutant (2006)
  • Author(s): Mitani H.
  • Organizer: SETAC(North America)27thAnnual Meeting
  • Place of Presentation: Montreal
• [Presentation] DNA修復、細胞周期、細胞死の制御におけるric1遺伝子の関与 (2006)
  • Author(s): 日高征幸, 尾田正二, 三谷啓志
  • Organizer: 日本放射線影響学会第49 回大会
  • Place of Presentation: 北海道
• [Book] The Medaka Genome : Why we need the multiple fish models in vertebrate functional genomics. Genome Dynamics vol2 (2006)
  • Author(s): itani,H., Kamei,Y., Fukamachi,S., Oda,S., Sasaki,T., Asakawa,S., Todo,T., Shimizu,N.
  • Publisher: "Structure and Evolution of Vertebrate Genomes" Edited by Volff JN. Karger Publishers Basel
• [Book] The Medaka Genome : Why we need the multiple fish models in vertebrate functional genomics. Genome Dynamics vol2. (2006)
  • Author(s): Mitani, H., 他
  • Total Pages: 17
  • Publisher: Karger Publishers Basel