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Study of Genome Stability of Germ Cells Using Medaka Mutants

Research Project

Project/Area Number 18310038
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Risk sciences of radiation/Chemicals
Research InstitutionThe University of Tokyo

Principal Investigator

MITANI Hiroshi  The University of Tokyo, 大学院・新領域創成科学研究科, 教授 (70181922)

Co-Investigator(Kenkyū-buntansha) ODA Shoji  東京大学, 大学院・新領域創成科学研究科, 講師 (50266714)
ISHIKAWA Tomoko  大阪大学, 大学院・医学系研究科, 助教 (70402922)
Project Period (FY) 2006 – 2008
Project Status Completed (Fiscal Year 2008)
Budget Amount *help
¥17,820,000 (Direct Cost: ¥15,300,000、Indirect Cost: ¥2,520,000)
Fiscal Year 2008: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2007: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Fiscal Year 2006: ¥6,900,000 (Direct Cost: ¥6,900,000)
Keywords放射線 / 動物 / 遺伝学 / ストレス / 遺伝子 / 細胞・組織 / メダカ / 突然変異 / 魚類
Research Abstract

放射線高感受性ric1細胞は、DNA2本鎖切断の修復が遅延しており、γ線による断片化を伴う細胞死が起きない。緑色蛍光蛋白質(GFP)遺伝子を生殖細胞特異的に発現するric1系統を用いたところ、卵割期にすでにric1生殖細胞は、放射線高感受性であり、生殖腺移行後に孵化率に影響を及ぼさない線量に対して大きな性差があることも分かった。また、酸化損傷修復に関わるOGG1遺伝子の突然変異ホモ接合体メダカを作製した。

Report

(4 results)
  • 2008 Annual Research Report   Final Research Report ( PDF )
  • 2007 Annual Research Report
  • 2006 Annual Research Report
  • Research Products

    (29 results)

All 2008 2007 2006

All Journal Article (16 results) (of which Peer Reviewed: 5 results) Presentation (11 results) Book (2 results)

  • [Journal Article] The cytotoxicity and genotoxicity of hexavalent chromium in medaka(Oryzias latipes)2008

    • Author(s)
      Goodale, B.C., R. Walter, S.R. Pelsue, W.D. Thompson, S.S. Wise, R.N. Winn, H. Mitani, J.P. Wise, Sr.
    • Journal Title

      Aquat Toxicol 87

      Pages: 60-67

    • Related Report
      2008 Final Research Report
  • [Journal Article] CYR61 is a novel gene associated with temperature-dependent changes in fish metabolism as revealed by cDNA microarray analysis on a medaka Oryzias latipes cell line2008

    • Author(s)
      Hirayama, M., M.N. Ahsan, H. Mitani, S. Watabe.
    • Journal Title

      J Cell Biochem 104

      Pages: 1297-1310

    • Related Report
      2008 Final Research Report
  • [Journal Article] DNA damage-induced ubiquitylation of RFC2 subunit of replication factor C complex2008

    • Author(s)
      Tomida J, Y.Masuda, H.Hiroaki, T.Ishikawa, I.Song , T.Tsurimoto, S.Tateishi, T. Shiomi, Y. Kamei, J. Kim, K. Kamiya, C. Vaziri, H. Ohmori, T. Todo
    • Journal Title

      J Biol Chem 283

      Pages: 9071-9079

    • Related Report
      2008 Final Research Report
  • [Journal Article] Development of a convenient in vitro fertilization method using interspecific hybrids between Oryzias latipes and Oryzias curvinotus2008

    • Author(s)
      Kamei Y, J.Itou, S. Oda, M. Masui, J.H.Kim, T. Ishikawa, S. Yuba, M. Kinoshita, H.Mitani, T. Todo
    • Journal Title

      J Biol Chem 283

      Pages: 9071-9079

    • NAID

      10019984185

    • Related Report
      2008 Final Research Report
  • [Journal Article] The cytotoxicity and genotoxicity of hexavalent chromium in medaka (Oryzias latipes) cells.2008

    • Author(s)
      Goodale, B. C
    • Journal Title

      Aquat Toxicol 87

      Pages: 60-67

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] CYR61 is a novel gene associated with temperature-dependent changes in fish metabolism as revealed by cDNA microarray analysis on a medaka Oryzias latipes cell line.2008

    • Author(s)
      Hirayama, M.
    • Journal Title

      J Cell Biochem 104

      Pages: 1297-1310

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] DNA damage-induced ubiquitylation of RFC2 subunit of replication factor C complex.2008

    • Author(s)
      Tomida, J.
    • Journal Title

      J Biol Chem. 283

      Pages: 9071-9079

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Development of a convenient in vitro fertilization method using interspecific hybrids between Oryzias latipes and Oryzias curvinotus2007

    • Author(s)
      Kamei Y, J.Itou, S. Oda, M. Masui, J.H.Kim, T. Ishikawa, S. Yuba, M. Kinoshita, H.Mitani, T. Todo
    • Journal Title

      Dev Growth Differ 49

      Pages: 721-730

    • NAID

      10019984185

    • Related Report
      2008 Final Research Report
  • [Journal Article] Radiation hybrid maps of Medaka chromosomes LG 12, 17, and 212007

    • Author(s)
      Su, F., et. al.
    • Journal Title

      DNA Res 14

      Pages: 135-140

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Deficiency in the response to DNA double-strand breaks inmouse early preimplantation embryos2007

    • Author(s)
      Yukawa, M., et. al.
    • Journal Title

      Biochem Biophys Res Commun 358

      Pages: 578-584

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Responses of embryonic germ cells of the radiation-sensitive Medaka mutant to γ-irradiation.2007

    • Author(s)
      Aizawa, K. 他
    • Journal Title

      J. Radat. Res. 48・2(in press)

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Generation of medaka gene knockout models by target-selected mutagenesis2006

    • Author(s)
      Taniguchi, Y., Takeda, S., Furutani-Seiki, M., Kamei, Y., Todo, T., Sasado, T., Deguchi, T., Kondoh, H., Mudde, J., Yamazoe, M., Hidaka, M., Mitani, H., Toyoda, A., Sakaki, Y., Plasterk, R. H., Cuppen, E.
    • Journal Title

      Genome Biol. 7

      Pages: 112-112

    • Related Report
      2008 Final Research Report
  • [Journal Article] Expression profiles of 4-nonylphenol-exposed medaka (O ryzias latipes) analyzed with a 3.4K microarray2006

    • Author(s)
      Kim, I. C., Lee, Y. M., Lee, C., Kim, H. M., Oda, S., Lee, Y. S., Mitani, H., Lee, J. S.
    • Journal Title

      Mar Environ Res. 62Suppl1

      Pages: 141-146

    • Related Report
      2008 Final Research Report
  • [Journal Article] The DNA sequence of medaka chromosome LG222006

    • Author(s)
      Sasaki, T., Shimizu, A., Ishikawa, S. K.,Imai, S.,Asakawa, S.,Murayama,Y., Khorasani, M. Z., Mitani, H., Furutani-Seiki, M., Kondoh, H., Nanda, I., Schmid, M., Schartl, M., Nonaka, M., Takeda, H.,Hori, H., Himmelbauer, H., Shima, A., Shimizu, N.
    • Journal Title

      Genomics 89

      Pages: 124-133

    • Related Report
      2008 Final Research Report
  • [Journal Article] The DNA sequence of medaka chromosome LG22.2006

    • Author(s)
      Sasaki, T., 他
    • Journal Title

      Genomics 89・1

      Pages: 124-133

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Generation of medaka gene knockout models by target-selected mutagenesis.2006

    • Author(s)
      Taniguchi, Y., 他
    • Journal Title

      Genome Biol. 7・12

      Pages: 112-112

    • Related Report
      2006 Annual Research Report
  • [Presentation] DNA損傷応答遺伝子ric1の機能解析.2008

    • Author(s)
      日高征幸
    • Organizer
      日本放射線影響学会
    • Place of Presentation
      北九州市
    • Year and Date
      2008-11-20
    • Related Report
      2008 Annual Research Report
  • [Presentation] Study of Genome Surveillance Mechanisms in Fish using Radiation Sensitive Medaka Mutant with defective repair of DNA double-strand breaks2008

    • Author(s)
      Mitani, H.
    • Organizer
      The 4th International Aquatic Animal Models of Human Disease Conference
    • Place of Presentation
      ダーラム米国
    • Year and Date
      2008-01-31
    • Related Report
      2007 Annual Research Report
  • [Presentation] Study of Genome Surveillance Mechanisms in Fish using Radiation Sensitive Medaka Mutant with Defective Repair of DNA Double-Strand Breaks2008

    • Author(s)
      Mitani H., Hidaka M., Yori K., Yamashita T., Nishino K., Urushihara U., Oda S.
    • Organizer
      The 4th International Aquatic Animal Models of Human Disease Conference(AAMHD)
    • Place of Presentation
      Duke University, Durham NC
    • Related Report
      2008 Final Research Report
  • [Presentation] DNA損傷応答遺伝子ric1の機能解析2008

    • Author(s)
      日高征幸, 尾田正二, 漆原佑介, 桑原義和, 福本学, 三谷啓志
    • Organizer
      日本放射線影響学会第51回
    • Place of Presentation
      北九州
    • Related Report
      2008 Final Research Report
  • [Presentation] メダカを用いたDNA二本鎖切断修復可視化2008

    • Author(s)
      漆原佑介, 尾田正二, 野田朝男, 小林純也, 小松賢志, 三谷啓志
    • Organizer
      日本放射線影響学会第51回
    • Place of Presentation
      北九州
    • Related Report
      2008 Final Research Report
  • [Presentation] Highly radiosensitive germ cells in Medaka ricl mutant with abnormal DNA double-strand break reair and otosis induction2007

    • Author(s)
      Aizawa, K., et. al.
    • Organizer
      The 13th Intemational Congress of Radiation Research
    • Place of Presentation
      サンフランシスコ米国
    • Year and Date
      2007-07-09
    • Related Report
      2007 Annual Research Report
  • [Presentation] The mutation of ricl induces delayed repair of DNA double strand breaks,cell death inhibition and ear checint release2007

    • Author(s)
      Hidaka, M., et. al.
    • Organizer
      The 13th Intemational Congress of Radiation Research
    • Place of Presentation
      サンフランシスコ米国
    • Year and Date
      2007-07-09
    • Related Report
      2007 Annual Research Report
  • [Presentation] Highly radiosensitive germ cells in Medaka ric1 mutant with abnormal DNA double-strand break repair and apoptosis induction2007

    • Author(s)
      Mitani H., Aizawa K., Yori K., Kaminaga C., Yashita T., Nishino K., Hidaka M., Kinoshita M., Oda S.
    • Organizer
      13th International Congress of Radiation Research
    • Place of Presentation
      San Francisco
    • Related Report
      2008 Final Research Report
  • [Presentation] The mutation of ric1 induces delayed repair of DNA double strand breaks, cell death inhibition and early checkpoint release2007

    • Author(s)
      Hidaka M., Oda S., Kuwahara Y., Fukumoto M., Mitani H.
    • Organizer
      13th International Congress of Radiation Research
    • Place of Presentation
      San Francisco
    • Related Report
      2008 Final Research Report
  • [Presentation] High radiosensitivity and abnormal apoptosis in germ cells of medaka ric1 mutant2006

    • Author(s)
      Mitani H.
    • Organizer
      SETAC(North America)27thAnnual Meeting
    • Place of Presentation
      Montreal
    • Related Report
      2008 Final Research Report
  • [Presentation] DNA修復、細胞周期、細胞死の制御におけるric1遺伝子の関与2006

    • Author(s)
      日高征幸, 尾田正二, 三谷啓志
    • Organizer
      日本放射線影響学会第49 回大会
    • Place of Presentation
      北海道
    • Related Report
      2008 Final Research Report
  • [Book] The Medaka Genome : Why we need the multiple fish models in vertebrate functional genomics. Genome Dynamics vol22006

    • Author(s)
      itani,H., Kamei,Y., Fukamachi,S., Oda,S., Sasaki,T., Asakawa,S., Todo,T., Shimizu,N.
    • Publisher
      "Structure and Evolution of Vertebrate Genomes" Edited by Volff JN. Karger Publishers Basel
    • Related Report
      2008 Final Research Report
  • [Book] The Medaka Genome : Why we need the multiple fish models in vertebrate functional genomics. Genome Dynamics vol2.2006

    • Author(s)
      Mitani, H., 他
    • Total Pages
      17
    • Publisher
      Karger Publishers Basel
    • Related Report
      2006 Annual Research Report

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Published: 2006-04-01   Modified: 2016-04-21  

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