Analysis of differential gene expression profiles induced by genotoxic and non-genotoxic carcinogens

• Project/Area Number: 18310047
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Risk sciences of radiation/Chemicals
• Research Institution: Aoyama Gakuin University
• Principal Investigator: FURIHATA Chie Aoyama Gakuin University, College of Science and Engineering, Professor (70012755)
• Co-Investigator(Kenkyū-buntansha): WATANABE Takashi Aoyama Gakuin University, College of Science and Engineering, Assistant Professor (50406815) ; SUZUKI Takayoshi National Institute of Health Sciences, 遺伝子細胞医薬部, Section Chief (30226526)
• Project Period (FY): 2006 – 2007
• Project Status: Completed (Fiscal Year 2007)
• Budget Amount: ¥6,540,000 (Direct Cost: ¥5,700,000、Indirect Cost: ¥840,000); Fiscal Year 2007: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2006: ¥2,900,000 (Direct Cost: ¥2,900,000)
• Keywords: Toxicogenomics / gene expression profile / DNA microarray / quantitative real-time PCR / genotoxic / non-genotoxic / carcinogen / mouse liver / qPCR

Research Abstract

The aim of this study is clarifying differential gene expression profiles and action mechanisms between genotoxic and non-genotoxic carcinogens in the target organ. The following results were obtained.
1)Differential gene expression profiles of 2 N-nitroso carcinogenic compounds, diethylnitrosamine and dipropylnitrosamine, and a non-genotoxic carcinogenic compound, phenobarbital, in mouse liver at 4 h and 28 days after administration determined with DNA microarray and quantitative real-time PCR.
2) Dose-dependent alteration in the gene expression in mouse liver induced by diethylnitrosamine and ethylnitrosourea at 4 h and 28 days after administration determined by quantitative real-time PCR.
3) Time-dependent gene expression profiles at 4, 16, 20, 24, and 48 h after administration of a genotoxic carcinogen, chrysene, in mouse liver determined with quantitative real-time PCR.
4) Differential gene expression profiles induced by 4 genotoxic carcinogens [quinoline, N-nitroso morpholine, 4-dimethyl (amino) azobenzene and 2, 4-diaminotoluene], and 2 non-genotoxic carcinogens (clofibrate and dichlorobenzene) in mouse liver at 4 and 48 h after administration determined with quantitative real-time PCR examined as training set.
5) Differential gene expression profiles induced by 4 genotoxic carcinogens [3, 4-(methylnitrosamino)-a-(3-pyridyl)-1-butanone, 2-actylamonofluorene, urethane, diisopropanolnitrosamine] and 3 non-genotoxic carcinogens [di(2-ethylhexyl)phthalate, dichloro-diphenyl-trichloroethane and furan] in mouse liver at 4 and 48 h after administration determined with quantitative real-time PCR examined as validation set.

Research Products

• [Journal Article] Differential gene expression induced by two N-nitroso carcinogens,phenobarbital and ethanol in mouse liver examined with oligonucleotide microarray and quantitative real-time PCR (2007)
  • Author(s): Takashi Watanabe, Kaori Tobe, Yutaka Nakachi, Yasumitsu Kondoh, Madoka Nakajima, Shuichi Hamada, Chiaki Namiki, Takayoshi Suzuki, Satoru Maeda, Ayami Tadakuma, Mikiya Sakurai, Yuko Arai, Hyogo Atsushi, Masako Hoshino, Tomoko Tashiro, Hisashi Ito, Hiroshige Inazumi, Yoshiyuki Sakaki, Hideo Tashiro, Chie Furihata
  • Journal Title: Genes and Environment 29 Pages: 115-127
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Differential gene expression induced by two N-nitroso carcinogens, phenobarbital and ethanol in mouse liver examined with oligonucleotide microarray and quantitative real-time PCR (2007)
  • Author(s): Takashi, Watanabe, Kaori, Tobe, Yutaka, Nakachi, Yasumitsu, Kondoh, Madoka, Nakajima, Shuichi, Hamada, Chiaki, Namiki, Takayoshi, Suzuki, Satoru, Maeda, Ayami, Tadakuma, Mikiya, Sakurai, Yuko, Arai, Hyogo, Atsushi, Masako, Hoshino, Tomoko, Tashiro, Hisashi, Ito, Hiroshige, Inazumi, Yoshiyuki, Sakaki, Hideo, Tashiro, Chie, Furihata
  • Journal Title: Genes and Environment 29 Pages: 115-127
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Differential gene expression indued by two N-nitroso carcinogens,phenobarbital and ethanol in mouse liver examined with oligonucleotide microarray and quantitative real-time PCR. (2007)
  • Author(s): Takashi Watanabe, Kaori Tobe, Yutaka Nakachi, Yasumitsu Kondoh, Madoka Nakajima, Shuichi Hamadai, Chiaki Namiki, Takayoshi Suzuki, Satoru Maeda, Ayam Tadakumui, Mikiya Sakural, Yuko.Arai, Hyogo Atsushi, Masako Hoshino, Tomoko Tashiro, Hisashi Ito, Hiroshige Inazumi, Yoshiyuki, Hideo Tashiro,Chie Furihata
  • Journal Title: Genes and Environment 29 Pages: 115-127
  • Peer Reviewed
• [Presentation] 遺伝子傷害性肝発がん物質のマウス肝臓における遺伝子発現解析 (2007)
  • Author(s): 多田隈 英未、櫻井 幹也、渡辺 貴志、鈴木 孝昌、濱田 修一、平山 道朝、真田 尚和、鈴木 洋、中嶋 圓、大信田 系裕、佐久間 智宏、降旗 千恵
  • Organizer: 第30回日本分子生物学会年会、第80回日本生化学会大会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2007-12-14
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] ジエチルニトロソアミンによるマウス肝臓における遺伝子発現変化の用量反応性 (2007)
  • Author(s): 新井 祐子、多田隈 英未、渡辺 貴志、濱田 修一、鈴木 孝昌、中嶋 圓、降旗 千恵
  • Organizer: 第30回日本分子生物学会年会、第80回日本生化学会大会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2007-12-14
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 遺伝子傷害性肝発がん物質Chryseneのマウス肝臓における遺伝子発現解析 (2007)
  • Author(s): 櫻井 幹也、多田隈 英未、花原 泉、渡辺 貴志、鈴木 孝昌、降旗 千恵
  • Organizer: 第30回日本分子生物学会年会、第80回日本生化学会大会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2007-12-14
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Analysis of gene expression profiles in mouse liver Induced by genotoxic carcinogens (2007)
  • Author(s): A, Tadakuma, M, Sakurai, T, Watanabe, T, Suzuki, S, Hamada, M, Hirayama, H, Sanada, H, Suzuki, M, Nakajima, K, Ohshita, T, Sanada, C, Furihata
  • Organizer: The 30th Annual meeting of the Molecular Biology Society of Japan & The 80th Annual Meeting of the Japanese Biochemical Society
  • Place of Presentation: Pacifico Yokohama
  • Year and Date: 2007-12-14
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Dose-dependent changes in gene expression profiles in mouse liver induced with diethyl-nitrosamine (2007)
  • Author(s): Y, Arai, A, Tadakuma, T, Watanabe, S, Hamada, T, Suzuki, M, Nakajima, C, Furihata
  • Organizer: The 30th Annual meeting of the Molecular Biology Society of Japan & The 80th Annual Meeting of the Japanese Biochemical Society
  • Place of Presentation: Pacifico Yokohama
  • Year and Date: 2007-12-14
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Time-dependent gene expression profiles in mouse liver induced with a genotoxic carcinogen, chrysene (2007)
  • Author(s): M, Sakurai, A, Tadakuma, I, Hanahara, T, Watanabe, T, Suzuki, C, Furihata
  • Organizer: The 30th Annual meeting of the Molecular Biology Society of Japan & The 80th Annual Meeting of the Japanese Biochemical Society
  • Place of Presentation: Pacifico Yokohama
  • Year and Date: 2007-12-14
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] 遺伝子傷害性肝発がん物質のマウス肝臓における遺伝子発現解析 (2007)
  • Author(s): 多田 隈英未、櫻井 幹也、渡辺 貴志、鈴木 孝昌、浜田 修一、平山 道朝、真田 尚和、鈴木 洋、中嶋 圓、大信 田系裕、佐久間 智宏、降旗 千恵
  • Organizer: 第30回日本分子生物学会年会・第80回日本生化学会大会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2007-12-14
• [Presentation] ジエチルニトロソアミンによるマウス肝臓における遺伝子発現変化の用量反応性 (2007)
  • Author(s): 新井 祐子、多田 隈英未、渡辺 貴志、濱田 修一、鈴木 孝昌、中嶋 圓、降旗 千恵
  • Organizer: 第30回日本分子生物学会年会・第80回日本生化学会大会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2007-12-14
• [Presentation] 遺伝子傷害性肝発がん物質Chryseneのマウス肝臓における遺伝子発現解析 (2007)
  • Author(s): 櫻井 幹也、多田 隈英未、花原 泉、渡辺 貴志、鈴木 孝昌、降旗 千恵
  • Organizer: 第30回日本分子生物学会年会・第80回日本生化学会大会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2007-12-14
• [Presentation] Collaborative study of JEMS/MMS/Toxicogenomics:Quantutative real-time PCR analysis on mouse liver carcinogens (2007)
  • Author(s): C Furihata, T Watanabe, A Tadakuma, M Sakurai, T Suzuki, S, Hamada, K Narumi, M Nakajima, A Koeda, T Sakuma, K Oshida, H Sanada and M Hirayama
  • Organizer: 1st Asian Conference on Environmental Mutagens&36th Annual Meeting of the Japanese Mutagen Society
  • Place of Presentation: Kitakyushu International Conference Center
  • Year and Date: 2007-11-29
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Collaborative study on the Toxicogenomics in JEMS/MMS II:High-throughput qPCR analysis by TagMan Low Density Array (2007)
  • Author(s): T Suzuki, T Koizumi, D Prabha, Y Luan, M Honma, S Hamada, M Nakajima, T Watanabe, C Furihata
  • Organizer: 1st Asian Conference on Environmental Mutagens&36th Annual Meeting of the Japanese Mutagen Society
  • Place of Presentation: Kitakyushu International Conference Center
  • Year and Date: 2007-11-29
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Collaborative study of JEMS/MMS/Toxicogenomics Quantutative real-time PCR analysis on mouse liver carcinogens (2007)
  • Author(s): C, Furihata, T Watanabe, A, Tadakuma, M, Sakurai, T, Suzuki, S, Hamada, K, Narumi, M, Nakajima, A, Koeda, T, Sakuma, K, Oshida, H, Sanada and M, Hirayama
  • Organizer: 1st Asian Conference on Environmental Mutagens & 36th Annual Meeting of the Japanese Mutagen Society
  • Place of Presentation: Kitakyushu International Conference Center
  • Year and Date: 2007-11-29
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Collaborative study on the Toxicogenomics in JEMS/MMS II : High-throughput qPCR analysis by TaqMan Low Density Array (2007)
  • Author(s): T, Suzuki, T, Koizumi, D, Prabha, Y, Luan, M, Honma, S, Hamada, M, Nakajima, T, Watanabe, C, Furihata
  • Organizer: 1st Asian Conference on Environmental Mutagens & 36th Annual Meeting of the Japanese Mutagen Society
  • Place of Presentation: Kitakyushu International Conference Center
  • Year and Date: 2007-11-29
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Collaborative study of JEMS/MMS/ToXicogenomics:Quantitative real-time PCR analysis on mouse liver carcinogens (2007)
  • Author(s): Chie Furihata, Takashi Watanabe, Ayami Tadakuma, Mikiya Sakurai, Takayoshi Suzuki, Shuichi, Hamada, Kazunori Narumi, Madoka Nakajima, Akiko Koeda, Tomohiro Sakuma, Keiyu Oshida, Hisakazu Sanada, Michiasa Hirayama
  • Organizer: 1^<st> Asian Conference on Environmental Mutagens & 36^<th> Annual Meeting of the Japanese Environmental Mutagen Society
  • Place of Presentation: 北九州国際会議場
  • Year and Date: 2007-11-29
• [Presentation] Collaborative study on the Toxicogenomics in JEMS/MMS II:Highthroughput qPCR analysis by TaqMan Low Density Array (2007)
  • Author(s): Takayoshi Suzuki, Tomoko Koizumi, Duraiswamy Prabha, Yang Luan, Masamitsuonma, Shuichi Hamada, Madoka Nakajima, Takashi watanabe, Chie Furihata
  • Organizer: 1^<st> Asian Conference on Environmental Mutagens & 36^<th> Annual Meeting of the Japanese Environ mental Mutagen Society
  • Place of Presentation: 北九州国際会議場
  • Year and Date: 2007-11-29
• [Presentation] Differentially expressed genes in mouse liver induced by N-nitroso carcinogenic compounds, phenobarbital and ethanol (2006)
  • Author(s): C Furihata, K Tobe, T Watanabe, S Maeda, M Hirayama, M Harada, M Nakajima, S Hamada, C Namiki, T Suzuki, Y Nakachi, Y Kondoh, T Tashiro and C Furihata
  • Organizer: The 20th IUBMB Congress and 11th FAOBMB Congress
  • Place of Presentation: Kyoto International Conference Hall
  • Year and Date: 2006-06-19
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Dose-dependent alteration in the expression of mouse hepatic genesinduced by diethylnitrosamine and ethylnitrosourea determined by quantitative RT-PCR (2006)
  • Author(s): T Watanabe, S Maeda, M Hirayama, S Hamada, C Namiki, M Nakajima, T Suzuki and C Furihata
  • Organizer: The 20th IUBMB Congress and 11th FAOBMB Congress
  • Place of Presentation: Kyoto International Conference Hall
  • Year and Date: 2006-06-19
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Dose-dependent alteration in the expression of mouse hepatic genes induced by diethylnitrosamine and ethylnitrosourea determined by quantitative RT-PCR (2006)
  • Author(s): T, Watanabe, S, Maeda, M, Hirayama, S, Hamada, C, Namiki, M, Nakajima, T, Suzuki and C, Furihata
  • Organizer: The 20th IUBMB Congress and 11th FAOBMB Congress
  • Place of Presentation: Kyoto International Conference Hall
  • Year and Date: 2006-06-19
  • Description: 「研究成果報告書概要(欧文)」より
• [Remarks] 「研究成果報告書概要(和文)」より
  • URL: http://www.chem.aoyama.ac.jp/Chem/ChemHP/Furihatalab/