Creation of novel photosynthetic microorganism for degradation of PCB in oligotrophic environment

• Project/Area Number: 18310050
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Environmental technology/Environmental materials
• Research Institution: Ibaraki University
• Principal Investigator: KIMURA Shigenobu Ibaraki University, 工学部, 教授 (90291608)
• Co-Investigator(Kenkyū-buntansha): 千田 俊域 (千田 俊哉) 産業総合研究所, 生物情報解析研究センター, 研究員 (30272868) ; 菓子野 康浩 兵庫県立大学大学院, 生命理学研究科, 助教 (20221872)
• Co-Investigator(Renkei-kenkyūsha): SENDA Toshiya 産業技術総合研究所, バイオメディシナル情報研究センター, 主任研究員 (30272868) ; KAHINO Yasuhiro 兵庫県立大学, 大学院・生命理学研究科, 准教授 (20221872)
• Research Collaborator: FUKUDA Masao 長岡技術科学大学, 工学部, 教授 (20134512) ; NISHIZAWA Akito 茨城大学, 産学連携イノベーション創成機構, 非常勤研究員 (30466643)
• Project Period (FY): 2006 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥16,670,000 (Direct Cost: ¥15,500,000、Indirect Cost: ¥1,170,000); Fiscal Year 2008: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2007: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000); Fiscal Year 2006: ¥11,600,000 (Direct Cost: ¥11,600,000)
• Keywords: 環境修復技術 / バイオレメディエーション / PCB / シアノバクテリア / 光合成 / タンパク質工学 / 基質特異性 / X 線結晶構造解析 / 電子伝達系 / フラビン酵素 / X線結晶構造解析 / 反応中間体 / 基質性異性 / 結晶化

Research Abstract

水や土壌中などに残留するポリ塩化ビフェニル(PCB)の汚染除去には、ビフェニル分解微生物を用いた生物的環境浄化が有効である.本研究では、PCB 分解細菌Pseudomonas sp. KKS102 株の分解酵素系タンパク質BphA1~A4 遺伝子を光合成微生物であるシアノバクテリア(ラン藻)に組み込んで、これまで困難であった貧栄養環境下でも光エネルギーを用いてビフェニル化合物を分解できる新規光合成微生物を創出した.

Research Products

• [Journal Article] Redox control of protein conformation in flavoproteins (2009)
  • Author(s): Senda, T., Senda, M., Kimura, S., Ishida, T
  • Journal Title: Antioxid Redox Signal 11 Pages: 1741-1766
  • Peer Reviewed
• [Journal Article] 反応中間体構造から明らかになった電子伝達タンパク質間の酸化還元依存的な親和性調節機構 (2008)
  • Author(s): 千田美紀, 木村成伸, 福田雅夫, 石田哲夫, 千田俊哉
  • Journal Title: 日本結晶学会誌 50 Pages: 341-347
  • NAID: 10023987813
• [Journal Article] 電子伝達タンパク質間の酸化還元状態依存的親和性調節機構 (2008)
  • Author(s): 千田美紀, 木村成伸, 福田雅夫, 石田哲夫, 千田俊哉
  • Journal Title: 化学と生物 46 Pages: 689-696
  • NAID: 10024334724
• [Journal Article] 電子伝達タンパク質間の酸化還元状態依存的親和性調節機構 (2008)
  • Author(s): 千田美紀
  • Journal Title: 化学と生物 46 Pages: 689-696
  • NAID: 10024334724
• [Journal Article] 反応中間体構造から明らかになった電子伝達タンパク質間の酸化還元依存的な親和性調節機構 (2008)
  • Author(s): 千田美紀
  • Journal Title: 日本結晶学会誌 50 Pages: 341-347
  • NAID: 10023987813
• [Journal Article] (2008)
  • Author(s): Akito Nishizawa
  • Journal Title: Inversion of NADH/NADPH-specificity of BphA4 from Pseudomonas sp. strain KK S102 by substitutions of Glu175 and Gln177. In Flavins and Flavoproteins 2008 (Fargo, S., Gomez-Moreno, C., Medina, M.)(Prensas Universitarias de Zaragoza, Spain) Pages: 273-278
• [Journal Article] (2008)
  • Author(s): Miki Senda
  • Journal Title: Molecular mechanism of the redox-depsndent interaction between NADH-dependent ferredoxin reductase and Rieske-type [2Fe-2S]ferredoxin. In Flavins and Flavooroteins 2008 (Fargo. S., Gomez-Moreno, C., Medina, M.)(Prensas Universitarias de Zaragoza, Spain) Pages: 113-118
• [Journal Article] Molecular mechanism of the redox-dependent interaction between NADH-dependent ferredoxin reductase and Rieske-type [2Fe-2S] ferredoxin (2007)
  • Author(s): Senda, M., Kishigami, S., Kimura, S., Fukuda, M., Ishida, T., Senda, T
  • Journal Title: J.Mol.Biol 373 Pages: 382-400
  • NAID: 10024334724
  • Peer Reviewed
• [Journal Article] Crystallization and preliminary X-ray analysis of the electron-transfer complex of Rieske-type [2Fe-2S] ferredoxin and NADH-dependent ferredoxin reductase derived from Acidovorax sp. Strain KKS102 (2007)
  • Author(s): Senda, M., Kishigami, S., Kimura, S., Senda, T
  • Journal Title: Acta Crystallogr.Sect.F 63 Pages: 520-523
  • Peer Reviewed
• [Journal Article] Crystallization and preliminary X-ray analysis of the reduced Rieske-type [2Fe-2S] ferredoxin derived from Pseudomonas sp. Strain KKS102 (2007)
  • Author(s): Senda, M., Kishigami, S., Kimura, S., Senda, T
  • Journal Title: Acta Crystallogr.Sect.F 63 Pages: 311-314
  • Peer Reviewed
• [Journal Article] Crystallization and perliminary X-ray analysis of the reduced Rieske-type [2Fe-2S] ferredoxin derived from Pseudomonas sp. strain KKS102. (2007)
  • Author(s): Miki Senda
  • Journal Title: Acta Crystallograph Sect F Struct Biol Cryst Commun. 63 Pages: 311-314
  • Peer Reviewed
• [Journal Article] Crystallization and preliminary X-ray analysis of the electron-transfer complex of Rieske-type [2Fe-2S] ferredoxin and NADH-dependent ferredoxin reductase derived from Acidovorax sp. strain KKS102. (2007)
  • Author(s): Miki Senda
  • Journal Title: Acta Crystallograph Sect F Struct Biol Cryst Commun. 63 Pages: 520-523
  • Peer Reviewed
• [Journal Article] Molecular mechanism of the redox-dependent interaction between NADH-dependent ferredoxin reductase and Rieske-type [2Fe-2S] ferredoxin. (2007)
  • Author(s): Miki Senda
  • Journal Title: J. Mol. Biol. 373 Pages: 382-400
  • NAID: 10024334724
  • Peer Reviewed
• [Journal Article] Crystallization and preliminary X-ray analysis of the Rieske-type [2Fe-2S] ferredoxin component of biphenyl dioxygenase from Pseudomonas sp. Strain KKS102 (2006)
  • Author(s): Senda, M., Kimura, S., Kishigami, S., Senda, T
  • Journal Title: Acta Crystallogr.Sect.F 62 Pages: 590-592
  • Peer Reviewed
• [Journal Article] Crystallization and preliminary X-ray analysis of the Tieske-type [2Fe-2S] ferredoxin component of biphenyl dioxygenase from Pseudomonas sp. strain KKS102. (2006)
  • Author(s): Miki Senda
  • Journal Title: Acta Crystallograph. Sect F Struct. Biol. Cryst. Commun. 62・6 Pages: 590-592
• [Presentation] Enzymatic properties of NADH/NADPH-specificity inverted BphA4 mutants from Pseudomonas sp. Strain KKS102 (2008)
  • Author(s): Nishizawa, A., Muramatsu, D., Kishigami, S., Kashino, Y., Senda, M., Senda, T., Fukuda, M., Kimura, S
  • Organizer: 第31回日本分子生物学会年会 第81回日本生化学会大会 合同大会 (BMB2008)
  • Place of Presentation: 神戸市神戸ポートアイランド
  • Year and Date: 2008-12-12
• [Presentation] 部位特異的ランダム変異導入によるNADPH 依存的BphA4 変異体の作製 (2008)
  • Author(s): 森重政, 村松大輔, 西澤明人, 千田美紀, 千田俊哉, 福田雅夫, 木村成伸
  • Organizer: 第31回日本分子生物学会年会 第81回日本生化学会大会 合同大会 (BMB2008)
  • Place of Presentation: 神戸市神戸ポートアイランド
  • Year and Date: 2008-12-12
• [Presentation] 部位特異的ランダム変異導入によるNADPH依存的BphA4変異体の作製 (2008)
  • Author(s): 森重政
  • Organizer: 第31回日本分子生物学会年会第81回日本生化学会大会合同大会(BMB2008)
  • Place of Presentation: 兵庫県神戸市神戸ポートアイランド神戸国際展示場
  • Year and Date: 2008-12-12
• [Presentation] Enzymatic properties of NADH/NADPH-specicifity inverted BphA4 mutants from Pseudomonas sp. strain KKS102 (2008)
  • Author(s): Nishizawa Akito
  • Organizer: 第31回日本分子生物学会年会第81回日本生化学会大会合同大会(BMB2008)
  • Place of Presentation: 兵庫県神戸市神戸ポートアイランド神戸国際展示場
  • Year and Date: 2008-12-12
• [Presentation] 電子伝達反応に伴って生じるFAD の構造変化が電子伝達タンパク質間の親和性を調節する (2008)
  • Author(s): 千田美紀, 木村成伸, 福田雅夫, 石田哲夫, 千田俊哉
  • Organizer: 第31回日本分子生物学会年会 第81回日本生化学会大会 合同大会 (BMB2008)
  • Place of Presentation: 神戸市神戸ポートアイランド
  • Year and Date: 2008-12-11
• [Presentation] 電子伝達反応に伴って生じるFADの構造変化が電子伝達タンパク質間の親和性を調節する (2008)
  • Author(s): 千田美紀
  • Organizer: 第31回日本分子生物学会年会第81回日本生化学会大会合同大会(BMB2008)
  • Place of Presentation: 兵庫県神戸市神戸ポートアイランド神戸国際展示場
  • Year and Date: 2008-12-11
• [Presentation] Molecular mechanism of the redox-dependent interaction between ferredoxin reductase and ferredoxin (2008)
  • Author(s): Senda, M., Kimura, S., Fukuda, M., Ishida, T., Senda, T
  • Organizer: XXI Congress and Genaral Assenbly of the International Union of Crystallography (IUCr2008)
  • Place of Presentation: Grand Cube Osaka, Osaka
  • Year and Date: 2008-08-28
• [Presentation] Molecular mechanism of the redox-dependent interaction between ferredo xin reductase and ferredoxin (2008)
  • Author(s): Miki Senda
  • Organizer: XXI Congress and Genaral Assenbly of the International Union of Cristallography (IUCr2008)
  • Place of Presentation: Grand Cube Osaka (Osaka In ternational Convention Center)
  • Year and Date: 2008-08-28
• [Presentation] 部位特異的ランダム変異導入によるNADPH 依存的BphA4 の選抜 (2008)
  • Author(s): 森重政, 村松大輔, 西澤明人, 千田俊哉, 千田美紀, 福田雅夫, 木村成伸
  • Organizer: 第8回日本蛋白質科学会年会
  • Place of Presentation: 東京都江戸川区タワーホール船堀
  • Year and Date: 2008-06-10
• [Presentation] 部位特異的ランダム変異導入によるNADPH依存的BphA4の選抜 (2008)
  • Author(s): 森重政
  • Organizer: 第8回日本蛋白質科学会年会
  • Place of Presentation: 東京都江戸川区タワーホール船堀
  • Year and Date: 2008-06-10
• [Presentation] Inversion of NADH/NADPH-specificity of BphA4 from Pseudomonas sp. Strain KKS102 by substitutions of Glu175 and Gln177 (2008)
  • Author(s): Nishizawa, A., Muramatsu, D., Kimura, S., Kishigami, S., Senda, M., Senda, T., Fukuda, M
  • Organizer: 16th Symposium on Flavins and Flavoproteins 2008
  • Place of Presentation: Palacio de Congressos, Jaca, Spain
  • Year and Date: 2008-06-09
• [Presentation] Molecular Mechanism of the redox-dependent interaction between NADH-dependent ferredoxin reductase and NADH/NAD^<+> (2008)
  • Author(s): Senda, M., Kimura, S., Fukuda, M., Ishida, T., Senda, T
  • Organizer: 16th Symposium on Flavins and Flavoproteins 2008
  • Place of Presentation: Palacio de Congressos, Jaca, Spain
  • Year and Date: 2008-06-09
• [Presentation] Molecular Mechanism of the redox-dependent interaction between NADH-de pendent ferredoxin reductase and NADH/NAD^+ (2008)
  • Author(s): Miki Senda
  • Organizer: 16th Symposium on Flavins and Flavoproteins 2008
  • Place of Presentation: Palacio de Congressos, Jaca, Spain
  • Year and Date: 2008-06-09
• [Presentation] Inversion of NADH/NADPH-specificity of BphA4 from Pseudomonas sp. stra in KKS102 by substitutions of Glu175 and Gln177 (2008)
  • Author(s): Akito Nishizawa
  • Organizer: 16th Symposium on Flavins and Flavoproteins 2008
  • Place of Presentation: Palacio de Congressos, Jaca, Spain
  • Year and Date: 2008-06-09
• [Presentation] Glu^<17>5およびGln^<177> の系統的置換によるPseudomonas sp. KKS102 株由来BphA4 のNADH/NADPH 特異性の逆転 (2007)
  • Author(s): 村松大輔, 岸上晋也, 上村卓哉, 千田俊哉, 菓子野康浩, 福田雅夫, 木村成伸
  • Organizer: 第30回日本分子生物学会年会 第80回日本生化学会大会 合同大会(BMB2007)
  • Place of Presentation: 横浜市パシフィコ横浜
  • Year and Date: 2007-12-15
• [Presentation] Glu^<175>およびGln^<177>の系統的置換によるPseudomonas sp. KKS102株由来BphA4のNADH/NADPH特異性の逆転 (2007)
  • Author(s): 村松大輔
  • Organizer: 第30回日本分子生物学会年会 第80回日本生化学会大会 合同大会(BMB2007)
  • Place of Presentation: 横浜市(パシフィコ横浜)
  • Year and Date: 2007-12-15
• [Book] Inversion of NADH/NADPH-specificity of BphA4 from Pseudomonas sp. strain KKS102 by substitutions of Glu^<175> and Gln^<177>. in Flavins and Flavoproteins 2008 (Fargo, S., Gomez-Moreno, C., Medina, M. eds) (2008)
  • Author(s): Nishizawa, A., Muramatsu, D., Kishigami, S., Senda, M., Senda, T., Fukuda, M., and Kimura, S
  • Publisher: Prensas Universitarias de Zaragoza, Spain
• [Book] Prensas Universitarias de Zaragoza, Spain (2008)
  • Author(s): Senda, M., Kimura, S., Fukuda, M., Ishida, T., and Senda, T
  • Publisher: Molecular mechanism of the redox-depsndent interaction between NADH-dependent ferredoxin reductase and Rieske-type [2Fe-2S] ferredoxin. In Flavins and Flavoproteins 2008 (Fargo, S., Gomez-Moreno, C., Medina, M. eds)
• [Remarks]
  • URL: http://info.ibaraki.ac.jp/scripts/websearch/index.htm
• [Remarks] 茨城大学研究者情報総覧
  • URL: http://info.ibaraki.ac.jp/scripts/websearch/index.htm