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Creation of novel photosynthetic microorganism for degradation of PCB in oligotrophic environment

Research Project

Project/Area Number 18310050
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Environmental technology/Environmental materials
Research InstitutionIbaraki University

Principal Investigator

KIMURA Shigenobu  Ibaraki University, 工学部, 教授 (90291608)

Co-Investigator(Kenkyū-buntansha) 千田 俊域 (千田 俊哉)  産業総合研究所, 生物情報解析研究センター, 研究員 (30272868)
菓子野 康浩  兵庫県立大学大学院, 生命理学研究科, 助教 (20221872)
Co-Investigator(Renkei-kenkyūsha) SENDA Toshiya  産業技術総合研究所, バイオメディシナル情報研究センター, 主任研究員 (30272868)
KAHINO Yasuhiro  兵庫県立大学, 大学院・生命理学研究科, 准教授 (20221872)
Research Collaborator FUKUDA Masao  長岡技術科学大学, 工学部, 教授 (20134512)
NISHIZAWA Akito  茨城大学, 産学連携イノベーション創成機構, 非常勤研究員 (30466643)
Project Period (FY) 2006 – 2008
Project Status Completed (Fiscal Year 2008)
Budget Amount *help
¥16,670,000 (Direct Cost: ¥15,500,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2008: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2007: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2006: ¥11,600,000 (Direct Cost: ¥11,600,000)
Keywords環境修復技術 / バイオレメディエーション / PCB / シアノバクテリア / 光合成 / タンパク質工学 / 基質特異性 / X 線結晶構造解析 / 電子伝達系 / フラビン酵素 / X線結晶構造解析 / 反応中間体 / 基質性異性 / 結晶化
Research Abstract

水や土壌中などに残留するポリ塩化ビフェニル(PCB)の汚染除去には、ビフェニル分解微生物を用いた生物的環境浄化が有効である.本研究では、PCB 分解細菌Pseudomonas sp. KKS102 株の分解酵素系タンパク質BphA1~A4 遺伝子を光合成微生物であるシアノバクテリア(ラン藻)に組み込んで、これまで困難であった貧栄養環境下でも光エネルギーを用いてビフェニル化合物を分解できる新規光合成微生物を創出した.

Report

(4 results)
  • 2008 Annual Research Report   Final Research Report ( PDF )
  • 2007 Annual Research Report
  • 2006 Annual Research Report
  • Research Products

    (35 results)

All 2009 2008 2007 2006 Other

All Journal Article (15 results) (of which Peer Reviewed: 8 results) Presentation (16 results) Book (2 results) Remarks (2 results)

  • [Journal Article] Redox control of protein conformation in flavoproteins2009

    • Author(s)
      Senda, T., Senda, M., Kimura, S., Ishida, T
    • Journal Title

      Antioxid Redox Signal 11

      Pages: 1741-1766

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] 反応中間体構造から明らかになった電子伝達タンパク質間の酸化還元依存的な親和性調節機構2008

    • Author(s)
      千田美紀, 木村成伸, 福田雅夫, 石田哲夫, 千田俊哉
    • Journal Title

      日本結晶学会誌 50

      Pages: 341-347

    • NAID

      10023987813

    • Related Report
      2008 Final Research Report
  • [Journal Article] 電子伝達タンパク質間の酸化還元状態依存的親和性調節機構2008

    • Author(s)
      千田美紀, 木村成伸, 福田雅夫, 石田哲夫, 千田俊哉
    • Journal Title

      化学と生物 46

      Pages: 689-696

    • NAID

      10024334724

    • Related Report
      2008 Final Research Report
  • [Journal Article] 電子伝達タンパク質間の酸化還元状態依存的親和性調節機構2008

    • Author(s)
      千田美紀
    • Journal Title

      化学と生物 46

      Pages: 689-696

    • NAID

      10024334724

    • Related Report
      2008 Annual Research Report
  • [Journal Article] 反応中間体構造から明らかになった電子伝達タンパク質間の酸化還元依存的な親和性調節機構2008

    • Author(s)
      千田美紀
    • Journal Title

      日本結晶学会誌 50

      Pages: 341-347

    • NAID

      10023987813

    • Related Report
      2008 Annual Research Report
  • [Journal Article]2008

    • Author(s)
      Akito Nishizawa
    • Journal Title

      Inversion of NADH/NADPH-specificity of BphA4 from Pseudomonas sp. strain KK S102 by substitutions of Glu175 and Gln177. In Flavins and Flavoproteins 2008 (Fargo, S., Gomez-Moreno, C., Medina, M.)(Prensas Universitarias de Zaragoza, Spain)

      Pages: 273-278

    • Related Report
      2008 Annual Research Report
  • [Journal Article]2008

    • Author(s)
      Miki Senda
    • Journal Title

      Molecular mechanism of the redox-depsndent interaction between NADH-dependent ferredoxin reductase and Rieske-type [2Fe-2S]ferredoxin. In Flavins and Flavooroteins 2008 (Fargo. S., Gomez-Moreno, C., Medina, M.)(Prensas Universitarias de Zaragoza, Spain)

      Pages: 113-118

    • Related Report
      2008 Annual Research Report
  • [Journal Article] Molecular mechanism of the redox-dependent interaction between NADH-dependent ferredoxin reductase and Rieske-type [2Fe-2S] ferredoxin2007

    • Author(s)
      Senda, M., Kishigami, S., Kimura, S., Fukuda, M., Ishida, T., Senda, T
    • Journal Title

      J.Mol.Biol 373

      Pages: 382-400

    • NAID

      10024334724

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Crystallization and preliminary X-ray analysis of the electron-transfer complex of Rieske-type [2Fe-2S] ferredoxin and NADH-dependent ferredoxin reductase derived from Acidovorax sp. Strain KKS1022007

    • Author(s)
      Senda, M., Kishigami, S., Kimura, S., Senda, T
    • Journal Title

      Acta Crystallogr.Sect.F 63

      Pages: 520-523

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Crystallization and preliminary X-ray analysis of the reduced Rieske-type [2Fe-2S] ferredoxin derived from Pseudomonas sp. Strain KKS1022007

    • Author(s)
      Senda, M., Kishigami, S., Kimura, S., Senda, T
    • Journal Title

      Acta Crystallogr.Sect.F 63

      Pages: 311-314

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Crystallization and perliminary X-ray analysis of the reduced Rieske-type [2Fe-2S] ferredoxin derived from Pseudomonas sp. strain KKS102.2007

    • Author(s)
      Miki Senda
    • Journal Title

      Acta Crystallograph Sect F Struct Biol Cryst Commun. 63

      Pages: 311-314

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Crystallization and preliminary X-ray analysis of the electron-transfer complex of Rieske-type [2Fe-2S] ferredoxin and NADH-dependent ferredoxin reductase derived from Acidovorax sp. strain KKS102.2007

    • Author(s)
      Miki Senda
    • Journal Title

      Acta Crystallograph Sect F Struct Biol Cryst Commun. 63

      Pages: 520-523

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Molecular mechanism of the redox-dependent interaction between NADH-dependent ferredoxin reductase and Rieske-type [2Fe-2S] ferredoxin.2007

    • Author(s)
      Miki Senda
    • Journal Title

      J. Mol. Biol. 373

      Pages: 382-400

    • NAID

      10024334724

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Crystallization and preliminary X-ray analysis of the Rieske-type [2Fe-2S] ferredoxin component of biphenyl dioxygenase from Pseudomonas sp. Strain KKS1022006

    • Author(s)
      Senda, M., Kimura, S., Kishigami, S., Senda, T
    • Journal Title

      Acta Crystallogr.Sect.F 62

      Pages: 590-592

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Crystallization and preliminary X-ray analysis of the Tieske-type [2Fe-2S] ferredoxin component of biphenyl dioxygenase from Pseudomonas sp. strain KKS102.2006

    • Author(s)
      Miki Senda
    • Journal Title

      Acta Crystallograph. Sect F Struct. Biol. Cryst. Commun. 62・6

      Pages: 590-592

    • Related Report
      2006 Annual Research Report
  • [Presentation] Enzymatic properties of NADH/NADPH-specificity inverted BphA4 mutants from Pseudomonas sp. Strain KKS1022008

    • Author(s)
      Nishizawa, A., Muramatsu, D., Kishigami, S., Kashino, Y., Senda, M., Senda, T., Fukuda, M., Kimura, S
    • Organizer
      第31回日本分子生物学会年会 第81回日本生化学会大会 合同大会 (BMB2008)
    • Place of Presentation
      神戸市神戸ポートアイランド
    • Year and Date
      2008-12-12
    • Related Report
      2008 Final Research Report
  • [Presentation] 部位特異的ランダム変異導入によるNADPH 依存的BphA4 変異体の作製2008

    • Author(s)
      森重政, 村松大輔, 西澤明人, 千田美紀, 千田俊哉, 福田雅夫, 木村成伸
    • Organizer
      第31回日本分子生物学会年会 第81回日本生化学会大会 合同大会 (BMB2008)
    • Place of Presentation
      神戸市神戸ポートアイランド
    • Year and Date
      2008-12-12
    • Related Report
      2008 Final Research Report
  • [Presentation] 部位特異的ランダム変異導入によるNADPH依存的BphA4変異体の作製2008

    • Author(s)
      森重政
    • Organizer
      第31回日本分子生物学会年会第81回日本生化学会大会合同大会(BMB2008)
    • Place of Presentation
      兵庫県神戸市神戸ポートアイランド神戸国際展示場
    • Year and Date
      2008-12-12
    • Related Report
      2008 Annual Research Report
  • [Presentation] Enzymatic properties of NADH/NADPH-specicifity inverted BphA4 mutants from Pseudomonas sp. strain KKS1022008

    • Author(s)
      Nishizawa Akito
    • Organizer
      第31回日本分子生物学会年会第81回日本生化学会大会合同大会(BMB2008)
    • Place of Presentation
      兵庫県神戸市神戸ポートアイランド神戸国際展示場
    • Year and Date
      2008-12-12
    • Related Report
      2008 Annual Research Report
  • [Presentation] 電子伝達反応に伴って生じるFAD の構造変化が電子伝達タンパク質間の親和性を調節する2008

    • Author(s)
      千田美紀, 木村成伸, 福田雅夫, 石田哲夫, 千田俊哉
    • Organizer
      第31回日本分子生物学会年会 第81回日本生化学会大会 合同大会 (BMB2008)
    • Place of Presentation
      神戸市神戸ポートアイランド
    • Year and Date
      2008-12-11
    • Related Report
      2008 Final Research Report
  • [Presentation] 電子伝達反応に伴って生じるFADの構造変化が電子伝達タンパク質間の親和性を調節する2008

    • Author(s)
      千田美紀
    • Organizer
      第31回日本分子生物学会年会第81回日本生化学会大会合同大会(BMB2008)
    • Place of Presentation
      兵庫県神戸市神戸ポートアイランド神戸国際展示場
    • Year and Date
      2008-12-11
    • Related Report
      2008 Annual Research Report
  • [Presentation] Molecular mechanism of the redox-dependent interaction between ferredoxin reductase and ferredoxin2008

    • Author(s)
      Senda, M., Kimura, S., Fukuda, M., Ishida, T., Senda, T
    • Organizer
      XXI Congress and Genaral Assenbly of the International Union of Crystallography (IUCr2008)
    • Place of Presentation
      Grand Cube Osaka, Osaka
    • Year and Date
      2008-08-28
    • Related Report
      2008 Final Research Report
  • [Presentation] Molecular mechanism of the redox-dependent interaction between ferredo xin reductase and ferredoxin2008

    • Author(s)
      Miki Senda
    • Organizer
      XXI Congress and Genaral Assenbly of the International Union of Cristallography (IUCr2008)
    • Place of Presentation
      Grand Cube Osaka (Osaka In ternational Convention Center)
    • Year and Date
      2008-08-28
    • Related Report
      2008 Annual Research Report
  • [Presentation] 部位特異的ランダム変異導入によるNADPH 依存的BphA4 の選抜2008

    • Author(s)
      森重政, 村松大輔, 西澤明人, 千田俊哉, 千田美紀, 福田雅夫, 木村成伸
    • Organizer
      第8回日本蛋白質科学会年会
    • Place of Presentation
      東京都江戸川区タワーホール船堀
    • Year and Date
      2008-06-10
    • Related Report
      2008 Final Research Report
  • [Presentation] 部位特異的ランダム変異導入によるNADPH依存的BphA4の選抜2008

    • Author(s)
      森重政
    • Organizer
      第8回日本蛋白質科学会年会
    • Place of Presentation
      東京都江戸川区タワーホール船堀
    • Year and Date
      2008-06-10
    • Related Report
      2008 Annual Research Report
  • [Presentation] Inversion of NADH/NADPH-specificity of BphA4 from Pseudomonas sp. Strain KKS102 by substitutions of Glu175 and Gln1772008

    • Author(s)
      Nishizawa, A., Muramatsu, D., Kimura, S., Kishigami, S., Senda, M., Senda, T., Fukuda, M
    • Organizer
      16th Symposium on Flavins and Flavoproteins 2008
    • Place of Presentation
      Palacio de Congressos, Jaca, Spain
    • Year and Date
      2008-06-09
    • Related Report
      2008 Final Research Report
  • [Presentation] Molecular Mechanism of the redox-dependent interaction between NADH-dependent ferredoxin reductase and NADH/NAD^<+>2008

    • Author(s)
      Senda, M., Kimura, S., Fukuda, M., Ishida, T., Senda, T
    • Organizer
      16th Symposium on Flavins and Flavoproteins 2008
    • Place of Presentation
      Palacio de Congressos, Jaca, Spain
    • Year and Date
      2008-06-09
    • Related Report
      2008 Final Research Report
  • [Presentation] Molecular Mechanism of the redox-dependent interaction between NADH-de pendent ferredoxin reductase and NADH/NAD^+2008

    • Author(s)
      Miki Senda
    • Organizer
      16th Symposium on Flavins and Flavoproteins 2008
    • Place of Presentation
      Palacio de Congressos, Jaca, Spain
    • Year and Date
      2008-06-09
    • Related Report
      2008 Annual Research Report
  • [Presentation] Inversion of NADH/NADPH-specificity of BphA4 from Pseudomonas sp. stra in KKS102 by substitutions of Glu175 and Gln1772008

    • Author(s)
      Akito Nishizawa
    • Organizer
      16th Symposium on Flavins and Flavoproteins 2008
    • Place of Presentation
      Palacio de Congressos, Jaca, Spain
    • Year and Date
      2008-06-09
    • Related Report
      2008 Annual Research Report
  • [Presentation] Glu^<17>5およびGln^<177> の系統的置換によるPseudomonas sp. KKS102 株由来BphA4 のNADH/NADPH 特異性の逆転2007

    • Author(s)
      村松大輔, 岸上晋也, 上村卓哉, 千田俊哉, 菓子野康浩, 福田雅夫, 木村成伸
    • Organizer
      第30回日本分子生物学会年会 第80回日本生化学会大会 合同大会(BMB2007)
    • Place of Presentation
      横浜市パシフィコ横浜
    • Year and Date
      2007-12-15
    • Related Report
      2008 Final Research Report
  • [Presentation] Glu^<175>およびGln^<177>の系統的置換によるPseudomonas sp. KKS102株由来BphA4のNADH/NADPH特異性の逆転2007

    • Author(s)
      村松大輔
    • Organizer
      第30回日本分子生物学会年会 第80回日本生化学会大会 合同大会(BMB2007)
    • Place of Presentation
      横浜市(パシフィコ横浜)
    • Year and Date
      2007-12-15
    • Related Report
      2007 Annual Research Report
  • [Book] Inversion of NADH/NADPH-specificity of BphA4 from Pseudomonas sp. strain KKS102 by substitutions of Glu^<175> and Gln^<177>. in Flavins and Flavoproteins 2008 (Fargo, S., Gomez-Moreno, C., Medina, M. eds)2008

    • Author(s)
      Nishizawa, A., Muramatsu, D., Kishigami, S., Senda, M., Senda, T., Fukuda, M., and Kimura, S
    • Publisher
      Prensas Universitarias de Zaragoza, Spain
    • Related Report
      2008 Final Research Report
  • [Book] Prensas Universitarias de Zaragoza, Spain2008

    • Author(s)
      Senda, M., Kimura, S., Fukuda, M., Ishida, T., and Senda, T
    • Publisher
      Molecular mechanism of the redox-depsndent interaction between NADH-dependent ferredoxin reductase and Rieske-type [2Fe-2S] ferredoxin. In Flavins and Flavoproteins 2008 (Fargo, S., Gomez-Moreno, C., Medina, M. eds)
    • Related Report
      2008 Final Research Report
  • [Remarks]

    • URL

      http://info.ibaraki.ac.jp/scripts/websearch/index.htm

    • Related Report
      2008 Annual Research Report
  • [Remarks] 茨城大学研究者情報総覧

    • URL

      http://info.ibaraki.ac.jp/scripts/websearch/index.htm

    • Related Report
      2007 Annual Research Report

URL: 

Published: 2006-04-01   Modified: 2016-04-21  

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