Construction of Protein detection Chips Using Peptide Arrays

• Project/Area Number: 18310087
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Nanomaterials/Nanobioscience
• Research Institution: Tokyo Institute of Technology
• Principal Investigator: MIHARA Hisakazu Tokyo Institute of Technology, 大学院・生命理工学研究科, 教授 (30183966)
• Co-Investigator(Kenkyū-buntansha): TOMIZAKI Kinya 東京工業大学, 大学院・生命理工学研究科, 助手 (90397026)
• Project Period (FY): 2006 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥17,100,000 (Direct Cost: ¥15,300,000、Indirect Cost: ¥1,800,000); Fiscal Year 2008: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2007: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2006: ¥9,300,000 (Direct Cost: ¥9,300,000)
• Keywords: ペプチド / タンパク質 / マイクロアレイ / ペプチドアレイ / プロテインチップ / 化合物ライブラリ / ライブラリ

Research Abstract

立体構造をデザインした種々のペプチド配列を同時合成し、デバイス配置可能な最新技術であるペプチドライブラリ手法を駆使し、種々のタンパク質を機能的特徴により解析することが可能なプロテイン検出チップ(デザインペプチドチップ)の開発を行った。デザインペプチドライブラリ構築、ドライペプチドアレイ構築、デザインペプチドアレイのデータの処理、および金基板を利用する新規ラベルフリー解析において成果をあげた。

Research Products

• [Journal Article] Poly(amidoamine)dendrimer modified gold surfaces for anomalous reflection of gold(AR)method to detect biomolecular interactions (2009)
  • Author(s): A. Syahir, K-Y. Tomizaki, K. Kajikawa, H. Mihara
  • Journal Title: Langmuir 25 Pages: 3667-3674
  • Peer Reviewed
• [Journal Article] Poly (amidoamine) dendrimer-modified gold surfaces for anomalous reflection of gold (AR) method to detect biomolecular interactions (2009)
  • Author(s): Amir Syahir
  • Journal Title: Langmuir (印刷中)
  • Peer Reviewed
• [Journal Article] Screening of α-Helical Peptide Ligands Controlling a Calcineurin Phosphatase Activity Bioorg (2007)
  • Author(s): K. Usui, K.-Y. Tomizaki, H. Mihara
  • Journal Title: Med. Chem. Lett 17 Pages: 167-171
  • Peer Reviewed
• [Journal Article] Interactions Between Peptides Containing Nucleobase Amino Acids and T7 Phages Displaying S. cerevisiae Proteins (2007)
  • Author(s): S. Watanabe, K.-Y. Tomizaki, T. Takahashi, K. Usui, K. Kajikawa, H. Mihara
  • Journal Title: Biopolymers(Peptide Science) 88 Pages: 131-140
  • Peer Reviewed
• [Journal Article] タンパク質分析用バイオチップ (2007)
  • Author(s): 三原久和
  • Journal Title: ケミカルエンジニアリング 52 Pages: 421-427
• [Journal Article] タンパク質検出チップ (2007)
  • Author(s): 富崎欣也、三原久和
  • Journal Title: 未来材料 7 Pages: 28-38
• [Journal Article] ペプチドライブラリを用いるタンパク質相互作用検出チップ (2007)
  • Author(s): 富崎欣也、臼井健二、三原久和
  • Journal Title: 電気化学および工業物理化学 75 Pages: 981-986
  • NAID: 10019676065
• [Journal Article] Screening of α-Helical Peptide Ligands Controlling a Calcineurin-Phosphatase Activity (2007)
  • Author(s): Kenji Usui
  • Journal Title: Bioorg. Med. Chem. Lett. 17 Pages: 167-171
  • Peer Reviewed
• [Journal Article] Interactions Between Peptides Containing Nucleobase Amino Acids and T7 Phages Displaying S. cerevisiae Proteins (2007)
  • Author(s): Shinya Watanabe
  • Journal Title: Biopolymers(Peptide Science) 88 Pages: 131-140
  • Peer Reviewed
• [Journal Article] Phosphate-Mediated Molecular Memory Driven by Two Different Protein Kinases as Information Input Elements (2007)
  • Author(s): Kin-ya Tomizaki
  • Journal Title: J. Am. Chem. Soc. 129 Pages: 8345-8352
  • Peer Reviewed
• [Journal Article] Rational design of homogenous protein kinase assay platforms that allow both fluorometric and colorimetric signal readouts (2006)
  • Author(s): K.-Y. Tomizaki, H. Mihara
  • Journal Title: Mol. BioSyst 2 Pages: 580-589
  • Peer Reviewed
• [Journal Article] Protein fingerprint data mining of a designed α-helical peptide array (2006)
  • Author(s): K. Usui, K.-Y. Tomizaki, H. Mihara
  • Journal Title: Mol. BioSyst 2 Pages: 417-420
  • Peer Reviewed
• [Journal Article] A PNA-DNA hybridization chip approach for the detection of β-secretase activity (2006)
  • Author(s): S. Sano, K.-Y. Tomizaki, K. Usui, H. Mihara
  • Journal Title: Bioorg. Med. Chem. Lett 16 Pages: 503-506
  • Peer Reviewed
• [Journal Article] A Novel Peptide Microarray for Protein Detection and Analysis Utilizing a Dry Peptide Array System (2006)
  • Author(s): K. Usui, K.-Y. Tomizaki, T. Ohyama, K. Nokihara, H. Mihara
  • Journal Title: Mol. BioSyst 2 Pages: 113-121
  • Peer Reviewed
• [Journal Article] A Novel Peptide Microarray for Protein Detection and Analysis Utilizing a Dry Peptide Array System (2006)
  • Author(s): Kenji Usui
  • Journal Title: Mol. BioSyst. 2 Pages: 113-121
• [Journal Article] Protein-fingerprint data mining of a designed □-helical peptide array (2006)
  • Author(s): Kenji Usui
  • Journal Title: Mol. BioSyst. 2 Pages: 417-420
• [Journal Article] Rational design of homogenous protein kinase assay platforms that allow both fluorometric and colorimetric signal readouts (2006)
  • Author(s): Kin-ya Tomizaki
  • Journal Title: Mol. BioSyst. 2 Pages: 580-589
• [Presentation] Designed peptide chips for protein analyses (2008)
  • Author(s): H. Mihara
  • Organizer: Korean Biochip Conference
  • Place of Presentation: Daejon, Korea
  • Year and Date: 2008-06-06
• [Presentation] 設計ペプチドを利用したタンパク質分析チップ (2007)
  • Author(s): 三原久和
  • Organizer: 第4回コンビナトリアル・バイオエンジニアリング会議
  • Place of Presentation: 大阪
  • Year and Date: 2007-11-09
• [Presentation] Designed Peptide Microarray for a Protein Chip Technology (2006)
  • Author(s): H. Mihara
  • Organizer: Systemic Innovation for Microbial Biotechnology
  • Place of Presentation: Busan. Korea
  • Year and Date: 2006-06-19
• [Book] Array Based Techniques Wiley Encyclopedia of Chemical Biology (2009)
  • Author(s): K.-Y. Tomizaki, K. Usui, H. Mihara Proteins(Ed. Begley, T. P.)
  • Publisher: John Wiley & Sons, Inc
• [Book] Designed Peptide Chip : Protein Fingerprinting Technology with a Dry Peptide Array and Statistical Data Mining, Peptide Microarrays (2009)
  • Author(s): K. Usui, K.-Y. Tomizaki, H. Mihara A
  • Publisher: Methods in Molecular Biology, Humana Press
• [Book] バイオ研究のフロンティア2、酵素・タンパク質をはかる・とらえる・利用する (2009)
  • Author(s): 岡畑恵雄、三原久和
  • Total Pages: 188
  • Publisher: 工学図書
• [Book] 超分子サイエンス第5節プロテインチップデバイス (2009)
  • Author(s): 臼井健二
  • Publisher: エヌ・ティー・エス(印刷中)
• [Book] とことんやさしいタンパク質の本、プロテインチップでタンパク質を検出 (2007)
  • Author(s): 三原久和、高橋剛、富崎欣也
  • Publisher: 日本工業新聞社
• [Book] ナノバイオ計測の実際 (2007)
  • Author(s): 三原久和、小畠英理、馬場嘉信
  • Total Pages: 224
  • Publisher: 講談社
• [Book] 新しいDNAチップの科学と応用(関根光雄編) (2007)
  • Author(s): 富崎欣也、三原久和
  • Publisher: 講談社サイエンティフィック
• [Book] ペプチドチップマイクロアレイ・バイオチップの最新技術 (2007)
  • Author(s): 富崎欣也、三原久和
  • Publisher: シーエムシー
• [Patent(Industrial Property Rights)] バイオセンシング用基板 (2008)
  • Inventor(s): 梶川浩太郎、三原久和、アミルシャヒルビンハムザー
  • Industrial Property Rights Holder: 東京工業大学
  • Filing Date: 2008-10-06
• [Patent(Industrial Property Rights)] センサ基板及びバイオセンサ (2007)
  • Inventor(s): 梶川浩太郎、三原久和、アミルシャヒルビンハムザー
  • Industrial Property Rights Holder: 東京工業大学
  • Filing Date: 2007-08-30