Large-scale DNA polymorphisms study of rice

• Project/Area Number: 18310136
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied genomics
• Research Institution: Iwate Biotechnology Research Center
• Principal Investigator: TERAUCHI Ryohei Iwate Biotechnology Research Center, 生命科学研究部, 研究部長 (50236981)
• Project Period (FY): 2006 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥17,730,000 (Direct Cost: ¥15,600,000、Indirect Cost: ¥2,130,000); Fiscal Year 2008: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2007: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2006: ¥8,500,000 (Direct Cost: ¥8,500,000)
• Keywords: 遺伝学 / ゲノム / 植物 / 進化 / 育種学

Research Abstract

イネゲノムDNA 変異の大規模解析を実施した。イネ染色体上の22 箇所の領域、計26kb のDNA 配列を、栽培イネOryza sativa とその近縁野生種の30 系統について決定した。DNA 変異の系統解析の結果、日本型イネとインド型イネは、野生種O. rufipogon から独立に起原したことが明らかになった。さらに、品種「ひとめぼれ」において、5000 系統の突然変異系統群を作出した。「ササニシキ」突然変異系統を用いて、いもち病菌真性抵抗性遺伝子Pia を同定した。

Research Products

• [Journal Article] Association genetics reveals three novel avirulence genes from the rice blast fungal pathogen Magnaportheoryzae (2009)
  • Author(s): Yoshida, K., Saitoh, H., Fujisawa, S., Kanzaki, H., Matsumura, H., Yoshida, K., Tosa, Y., Chuma, I., Takano, Y., Win, J., Kamoun, S., Terauchi, R.
  • Journal Title: Plant Cell (in press)
• [Journal Article] SuperSAGE : The most advanced transcriptome technology for functional genomics (In : Kahl, G., Meksem, K. eds. The Handbook of Plant Functional Genomics) (2008)
  • Author(s): Terauchi Ryohei, et al.
  • Journal Title: Wiley-VCH Pages: 37-54
• [Journal Article] SuperSAGE : A modern platform for genome-wide quantitative transcript profiling (2008)
  • Author(s): Matsumura, H., Krueger, DH, Kahl, G., Terauchi, R
  • Journal Title: Current Pharmaceutical Biotechnology 9 Pages: 368-374
  • Peer Reviewed
• [Journal Article] A multi-faceted genomics approach toward understanding Magnaporthe-rice interactions (2008)
  • Author(s): Terauchi Ryohei
  • Journal Title: Biology of Molecular Plant-Microbe Interactions 6(in press)
• [Journal Article] Large-scale DNA polymorphism study of Oryza sativa and O. rufipogon reveals the origin and divergence of Asian rice. (2007)
  • Author(s): Rakshit, S., Rakshit , A., Matsumura, H., Takahashi, Y., Hasegawa, Y., Ito, A., Ishii, T., Miyashita, N. T., Terauchi, R.
  • Journal Title: Theoretical and Applied Genetics 114 Pages: 731-743
• [Journal Article] Large-scale DNA polymorphism study of Oryza sativa and O. rufipogon reveals the origin and divergence of Asian rice. (2007)
  • Author(s): Raksit, Sujay
  • Journal Title: Theoretical and Applied Genetics 114・4 Pages: 731-743
• [Presentation] Genome resequencing and association genetics reveals novel Magnaporthe oryzae avirulence genes. (2008)
  • Author(s): Terauchi,R.
  • Organizer: Biotechnology Havana 2008
  • Place of Presentation: Havana, Cuba
  • Year and Date: 2008-12-02
• [Presentation] Genome resequencing and association genetics reveals novel Magnaporthe oryzae avirulence genes (2008)
  • Author(s): 寺内良平
  • Organizer: Biotechnology Havana2008
  • Place of Presentation: Havana, Cuba
  • Year and Date: 2008-12-01
• [Presentation] Rice-Magnaporthe interactions:a multifaceted genomics approach (2007)
  • Author(s): 寺内 良平
  • Organizer: 5th International Symposium of Rice Functional Genomics
  • Place of Presentation: つくば市エポカルつくば
  • Year and Date: 2007-10-15
• [Presentation] A multi-faceted genomics approach toward understanding Magnaporthe-rice interactions (2007)
  • Author(s): 寺内 良平
  • Organizer: 13th International Congress on Molecular Plant・Microbe Interactions
  • Place of Presentation: Sorrento,Italy
  • Year and Date: 2007-07-25
• [Presentation] Searching for effectors of Magnaporthe oryzae: a multi-faceted genomics approach (2007)
  • Author(s): Terauchi,R.
  • Organizer: 4th International Rice Blast Conference
  • Place of Presentation: Changsha,China
• [Presentation] Searching for effectors of Magnaporthe oryzae: a multi-faceted genomics approach (2007)
  • Author(s): Terauchi,R.
  • Organizer: 5th International Symposium of Rice Functional Genomics
  • Place of Presentation: Tsukuba, Japan
• [Book] Use of TILLING for reverse and forward genetics of rice (In : Kahl, G., Meksem, K. eds. The Handbook of Plant Mutation Screening) (2009)
  • Author(s): Rakshit S., Terauchi, R
  • Publisher: Wiley-VCH(In press)
• [Book] A multi-faceted genomics approach toward understanding Magnaporthe-rice interactions. In: Biology of Molecular Plant-Microbe Interactions.(Lorito M. ed.) Vol 6. (2008)
  • Author(s): Terauchi, R., Win, J., Kamoun, S., et al.
• [Book] SuperSAGE: The most advanced transcriptome technology for functional genomics. In:Handbook of plant functional genomics.(Kahl, G., Meksam, K. eds) (2007)
  • Author(s): Terauchi, R., Matsumura, H., Krueger, D. H., Kahl, G.
  • Publisher: Wiley VCH
• [Book] Use of Serial Analysis of Gene Expression in crop Improvement. Genomics-Assisted Crop Improvement.(Varshney and Tuberosa eds) (2007)
  • Author(s): Sharma, P.C., Matsumura, H., Terauchi, R.
  • Publisher: Springer
• [Book] Use of TILLING for forward genetics of rice 2009 In:Handbook of plant mutation detection (Kahl, G., Meksam, K. eds)
  • Author(s): Rakshit, S., Kanzaki, H., Matsumura H., et al. Terauchi R.
  • Publisher: Wiley VCH (in press)