Inactivation of Tumor Suppressor Protein p53 by Mutations in the Tetramerization Domain and Stabilizing Methods for the Tetrameric Structure

• Project/Area Number: 18310140
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Living organism molecular science
• Research Institution: Hokkaido University
• Principal Investigator: SAKAGUCHI Kazuyasu Hokkaido University, 大学院・理学研究院, 教授 (00315053)
• Co-Investigator(Kenkyū-buntansha): TOSHIAKI Imagawa 北海道大学, 大学院・先端生命科学研究院, 准教授 (20142177) ; CHUMAN Yoshiro 北海道大学, 大学院・理学研究院, 助教 (40372263)
• Project Period (FY): 2006 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥17,350,000 (Direct Cost: ¥15,100,000、Indirect Cost: ¥2,250,000); Fiscal Year 2008: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2007: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2006: ¥7,600,000 (Direct Cost: ¥7,600,000)
• Keywords: 構造活性相関 / 蛋白質 / 癌 / バイオテクノロジー / 生体分子 / 癌抑制タンパク質 / 変異体 / オリゴマー / ペプチド / フォールデイング / フォールディング

Research Abstract

がん抑制遺伝子p53の変異は、ヒト悪性腫瘍で最も多く報告されている異常である。ヒトp53はリン酸化タンパク質であり、そのがん抑制機能の発現のためには四量体の形成が必須である。本研究では、四量体形成ドメイン変異や修飾によるp53機能の不活性化機構と変異の閾値を明らかとした。さらに、p53四量体ドメインに対する安定化法および機能修復ペプチドの開発を実施した。

Research Products

• [Journal Article] Oxidation of methionine residue at hydrophobic core destabilizes p53 tetrameric structure (2009)
  • Author(s): T. Nomura, R. Kamada, I. Ito, Y.Chuman, Y. Shimohigashi, K. Sakaguchi
  • Journal Title: Biopolymers 91(1) Pages: 78-84
  • Peer Reviewed
• [Journal Article] Effects of tumor-associated mutations in thep53 tetramerization domain onoligomerization state and transcriptionalactivity (2009)
  • Author(s): R. Kamada, T. Terai, T. Nomura, Y. Chuman, T. Imagawa, K. Sakaguchi
  • Journal Title: Adv. Exp.Med. Biol 611 Pages: 567-568
  • Peer Reviewed
• [Journal Article] Thermal Stability of p53 Tetramerization Domain Peptides Derived from Various Species (2009)
  • Author(s): T. Nomura, R. Kamada, Y. Chumanand and K. Sakaguchi
  • Journal Title: Peptide Sci 2008 Pages: 35-36
  • NAID: 10027165876
  • Peer Reviewed
• [Journal Article] Evaluation of Transcriptional Activity of p53 in Individual Living Mammalian Cell (2009)
  • Author(s): T.Imagawa, T. Terai, Y. Yamada, R. Kamada and K. Sakaguchi
  • Journal Title: Anal. Biochem. 387 Pages: 249-256
  • Peer Reviewed
• [Journal Article] Oxidation of Methionine Residue at Hydrophobic Core Destabilizes p53 Tetrameric Structure (2009)
  • Author(s): T. Nomura
  • Journal Title: Biopolymers 91 Pages: 78-84
  • Peer Reviewed
• [Journal Article] Evaluation of transcriptional activity of p53 in individual living mammalian cells (2009)
  • Author(s): T. Imagawa
  • Journal Title: Anal. l Biochem. 387 Pages: 249-256
  • Peer Reviewed
• [Journal Article] PPMID430, a Novel Alternative Splicing Variant of the Human PPM1D, can Dephosphorylate p53 and Exhibits Specific Tissue Expression (2009)
  • Author(s): Y. Chuman
  • Journal Title: J. Biochem. 145 Pages: 1-12
  • Peer Reviewed
• [Journal Article] Thermal Stability of p53 Tetramerization Domain Peptides Derived from Various Species (2009)
  • Author(s): T. Nomura
  • Journal Title: Peptide Science 2008 Pages: 35-36
  • Peer Reviewed
• [Journal Article] PPM1D430, a Novel Alternative Splicing Variant of the Human PPM1D. (2009)
  • Author(s): Y. Chuman
  • Journal Title: Peptide Science 2008 Pages: 531-532
  • Peer Reviewed
• [Journal Article] Identification of Novel Chemical Inhibitors for p53-Inducible Protein Phosphatase PPM1D (2009)
  • Author(s): H. Yagi
  • Journal Title: Peptide Science 2008 Pages: 385-386
  • Peer Reviewed
• [Journal Article] Extremely Long Nanofibrils of Functionalized Structure-Controlled-Amyloid Peptides Derived from Transthyretin (2009)
  • Author(s): H. Sakai
  • Journal Title: Peptide Science 2008 Pages: 465-466
  • Peer Reviewed
• [Journal Article] Differential Recognition of PhosphorylatedTransactivation Domains of p53 by Differentp300 Domains (2008)
  • Author(s): S. Polley, S. Guha, N.S. Roy,S. Kar, K. Sakaguchi, Y. Chuman, V.Swaminathan and S. Roy
  • Journal Title: J. Mol. Biol. 376 Pages: 8-12
  • Peer Reviewed
• [Journal Article] Correlation of Oligomeric Structure and Transcriptional Activity of p53 Mutants (2008)
  • Author(s): R. Kamada, T. Terai, T. Nomura, Y. Chuman, T.Imagawa and K. Sakaguchi
  • Journal Title: Peptide Sci 2007 Pages: 69-70
  • NAID: 10021906623
  • Peer Reviewed
• [Journal Article] Screening of Peptides to Stabilize the p53 Tetramer Formation from Phage Displayed Library (2008)
  • Author(s): T. Nomura, R. Kamada, Y. Chuman and K. Sakaguchi
  • Journal Title: Peptide Sci 2007 Pages: 315-316
  • NAID: 10021907074
  • Peer Reviewed
• [Journal Article] Correlation between Expression Level in E.coli and Stability of Oligomeric Structure ofthe p53 Tetramerization Domain (2008)
  • Author(s): H. Miyazaki, R. Kamada, T. Nomura, Y. Chuman, T.Imagawa and K. Sakaguchi
  • Journal Title: Peptide Sci 2007 Pages: 317-318
  • Peer Reviewed
• [Journal Article] Structural Photomodulation of Peptides with Azobenzoate Derivative in Peptide Backbone. (2008)
  • Author(s): R. Kamada, K. Fukuda, H. Miyazaki, T.Nomura, Y. Chuman, T. Imagawa, K. Tanino and K. Sakaguchi
  • Journal Title: Peptide Sci 2007 Pages: 447-448
  • NAID: 10021907298
  • Peer Reviewed
• [Journal Article] Characterization of the Active Site and a Unique Uncompetitive Inhibitor of the PPM1-tvpe Protein Phosphatase PPM1D (2008)
  • Author(s): Y. Chuman
  • Journal Title: Protein Pept. Lett. 15 Pages: 938-948
  • Peer Reviewed
• [Journal Article] Differential Recognition of Phosphorylated Transactivation Domains of p53 by Different p300 Domains. (2008)
  • Author(s): S. Polley
  • Journal Title: J. Mol. Biol. 371 Pages: 8-12
  • Peer Reviewed
• [Journal Article] Characterization of the Active Site and a Unique Uncompetitive Inhibitor of the PPM1-type Protein Phosphatase PPM1D (2008)
  • Author(s): Y. Chuman
  • Journal Title: Protein & Peptide Lett. (in press)
  • Peer Reviewed
• [Journal Article] Correlation of Oligomeric Structure and Transcriptional Activity of p53 Mutants (2008)
  • Author(s): K. Sakaguchi
  • Journal Title: Peptide Science 2007 Pages: 69-70
  • NAID: 10021906623
• [Journal Article] Screening of Peptides to Stabilize the p53 Tetramer Formation from Phage Displayed Library (2008)
  • Author(s): T. Nomura
  • Journal Title: Peptide Science 2007 Pages: 315-316
• [Journal Article] Correlation between expression level in E. coli and Stability of oligomeric structure of the p53 tetramerization domain (2008)
  • Author(s): H. Miyazaki
  • Journal Title: Peptide Science 2007 Pages: 317-318
• [Journal Article] Structural photomodulation of peptides with azobenzoate derivative in peptide backbone (2008)
  • Author(s): R. Kamada
  • Journal Title: Peptide Science 2007 Pages: 447-448
• [Journal Article] Stabilization of p53 Tetrameric Structure by Polyol Compounds (2007)
  • Author(s): T. Nomura, Y. Chuman and K. Sakaguchi
  • Journal Title: Peptide Sci 2006 Pages: 131-132
  • NAID: 10020529267
  • Peer Reviewed
• [Journal Article] Thermodynamic Stability of Mutant p53 Tetramerization Domain Peptides Associated with Human Tumors (2007)
  • Author(s): R. Kamada, T. Nomura, Y. chuman, T. Imagawa and K. Sakaguchi
  • Journal Title: Peptide Sci 2006 Pages: 131-132
  • NAID: 10020530042
  • Peer Reviewed
• [Journal Article] Unfolding, Aggregation, and Amyloid Formation by the Tetramerization Domain from Mutant p53 Associated with Lung Cancer (2006)
  • Author(s): Y. Higashimoto, Y. Asanomi, S.Takakusagi, M. S. Lewis, K. Uosaki, S. R. Durell, C. W. Anderson, E. Appella and K. Sakaguchi
  • Journal Title: Biochemistry 45 Pages: 1608-1619
  • Peer Reviewed
• [Journal Article] Analysis of Stabilization by Substituti on of Cyclohexylalanine for Phe341 in Hydrophobic Core of p53 Tetramer. (2006)
  • Author(s): T. Nomura, K. Sakamoto, Y. Kasai, Y. Chuman, K. Ishimori and K. Sakaguchi
  • Journal Title: Peptide Science 2005 Pages: 399-402
  • Peer Reviewed
• [Journal Article] Fibrillar and Granular Aggregation of G334V Mutant p53 Tetramer Peptide. Y. Asanomi, Y. Higashimoto (2006)
  • Author(s): Y. Asanomi, Y. Higashimoto, Y. Chuman, S. Kaya, T. Imagawa and K. Sakaguchi
  • Journal Title: Peptide Sci 2005 Pages: 397-398
  • Peer Reviewed
• [Journal Article] Unfolding, Aggregation, and Amyloid Formation by the Tetramerization Domain from Mutant p53 Associated with Lung Cancer (2006)
  • Author(s): Y.Higashimoto
  • Journal Title: Biochemistry 45 Pages: 1608-1619
• [Journal Article] Thermodynamic Stability of Mutant p53 Tetramerizaation Domain Peptides Associated with Human Tumors (2006)
  • Author(s): R.Kamada
  • Journal Title: Peptide Science 2006 Pages: 293-294
• [Journal Article] Stabilization of p53 Tetrameric Structure by Polyol Compounds (2006)
  • Author(s): T.Nomura
  • Journal Title: Peptide Science 2006 Pages: 131-132
• [Journal Article] Structural isoforms of the circadian neuropeptide PDF expressed in the optic lobes of the cricket Gryllus bimaculatus : immunocytochemical evidence from specific monoclonal antibodies (2006)
  • Author(s): T.Honda
  • Journal Title: J. Comp. Neurol. 499 Pages: 404-421
• [Journal Article] New Evidence for ATP Binding Induced Catalytic Subunit Interactions in Pig Kidney Na/K-ATPase (2006)
  • Author(s): K.Tanoue
  • Journal Title: J. Biochem. 140 Pages: 599-607
• [Presentation] Functional Bionanomaterial Based on Peptide Self-assembly (2008)
  • Author(s): K. Sakaguchi
  • Organizer: International Symposium on Molecular and System Life Sciences
  • Place of Presentation: 理研・神戸
  • Year and Date: 2008-12-11
• [Presentation] Identification and characterization of novel alternative splicing variant of p53-inducible protein phosphatase PPMID (2008)
  • Author(s): W. Kurihashi
  • Organizer: 第8回ホスファターゼカンファレンス
  • Place of Presentation: 前橋テルサ・群馬
  • Year and Date: 2008-11-14
• [Presentation] Thermal stability of p53 tetramerization domain peptides derived from various species (2008)
  • Author(s): T. Nomura, et. al.
  • Organizer: 第45回ペプチド討論会
  • Place of Presentation: 船堀
  • Year and Date: 2008-10-29
• [Presentation] Chemical and Structural Proteomics : The Solution to Biological Problems (2008)
  • Author(s): K. Sakaguchi
  • Organizer: MPSA2008
  • Place of Presentation: Sapporo
  • Year and Date: 2008-08-26
• [Presentation] Effect of thermodynamic stability on expression level of p53 tetramerization domain peptides in E. coli (2008)
  • Author(s): H. Miyazaki, et. al.
  • Organizer: MPSA2008
  • Place of Presentation: Sapporo
  • Year and Date: 2008-08-26
• [Presentation] Analysis of stability for p53 tetramerization domain peptides derived from various species (2008)
  • Author(s): T. Nomura, et. al.
  • Organizer: MPSA2008
  • Place of Presentation: Sapporo
  • Year and Date: 2008-08-26
• [Presentation] Tetramerization of tumor suppressor protein p53 (2008)
  • Author(s): K. Sakaguchi
  • Organizer: 12th Akabori Conference : German-Japanese Symposium on Peptide Science
  • Place of Presentation: Kyoto
  • Year and Date: 2008-05-16
• [Presentation] Tetramerization Domain of Tumor Suppressor Protein p53 (2008)
  • Author(s): K. Sakaguchi
  • Organizer: Taiwan-Japan International Symposium on Organic Chemistry and Molecular Science
  • Place of Presentation: Taipei, Taiwan
  • Year and Date: 2008-04-18
• [Presentation] Tetramerization Domain of Tumor Suppressor Protein p53 (2008)
  • Author(s): K. Sakaguchi
  • Organizer: Taiwan-Japan International Symposium on Organic Chemistry and Molecular Science
  • Place of Presentation: 台北・台湾
  • Year and Date: 2008-04-18
• [Presentation] Effects of Aromaticity in Hydrophobic Core on Stability of Tetramerization Domain in Tumor Suppressor Protein p53 (2008)
  • Author(s): T. Nomura
  • Organizer: Taiwan-Japan International Symposium on Organic Chemistry and Molecular Science
  • Place of Presentation: 台北・台湾
  • Year and Date: 2008-04-18
• [Presentation] 癌抑制タンパク質p53四量体形成ドメインペプチドの空隙充填による自己組織可能への影響 (2008)
  • Author(s): 野村尚生, 他
  • Organizer: 分子情報生命科学シンポジウム2008
  • Place of Presentation: 札幌
  • Year and Date: 2008-02-29
• [Presentation] ターン部位にアゾベンゼンアミノ酸誘導体を導入したペプチドの光による構造制御 (2008)
  • Author(s): 中馬吉郎, 他
  • Organizer: 分子情報生命科学シンポジウム2008
  • Place of Presentation: 札幌
  • Year and Date: 2008-02-29
• [Presentation] ターン部位にアゾベンゼンアミノ酸誘導体を導入したペプチドの光による構造制御 (2008)
  • Author(s): 中馬 吉郎
  • Organizer: 分子情報生命科学シンポジウム2008
  • Place of Presentation: 北海道大学(北海道)
  • Year and Date: 2008-02-29
• [Presentation] 癌抑制タンパク質p53四量体形成ドメインペプチドの空隙充填による自己組織可能への影響 (2008)
  • Author(s): 野村 尚生
  • Organizer: 分子情報生命科学シンポジウム2008
  • Place of Presentation: 北海道大学(北海道)
  • Year and Date: 2008-02-29
• [Presentation] 大腸菌発現量におけるペプチド構造安定性の効果 (2008)
  • Author(s): 宮崎広充, 他
  • Organizer: 北海道支部2008 年冬季研究発表会
  • Place of Presentation: 札幌
  • Year and Date: 2008-01-29
• [Presentation] 癌抑制タンパク質p53の四量体構造-転写活性の相関解析 (2008)
  • Author(s): 鎌田瑠泉
  • Organizer: 日本分子生物学会第8回春季シンポジウム
  • Place of Presentation: 京王プラザホテル札幌
• [Presentation] 癌抑制タンパク質p53誘導性ホスファターゼのAlternative splicing varlant (2008)
  • Author(s): 中馬吉郎
  • Organizer: 日本分子生物学会第8回春季シンポジウム
  • Place of Presentation: 京王プラザホテル・札幌
• [Presentation] Tetramerization of Tumor Suppressor Protein p53 (2008)
  • Author(s): K. Sakaguchi
  • Organizer: 12th Akabori Conference German - Japanese Symposium on Peptide Science
  • Place of Presentation: 京都プラザホテル・京都
• [Presentation] Effects of thermodynamic stability on expression Level of p53 tetramerizatiim domain peptide in E.coli (2008)
  • Author(s): H. Miyazaki
  • Organizer: 17th Meeting of Methods in Protein Structure Analysis
  • Place of Presentation: 北海道大学学術交流会館・札幌
• [Presentation] Quantitative analysis of oligomeric stability and transcriptional activity of tumor suppressor protein p53 (2008)
  • Author(s): R. Ramada
  • Organizer: 17th Meeting of Methods in Protein Structure Analysis
  • Place of Presentation: 北海道大学学術交流会館・札幌
• [Presentation] Analysis of stability for p53 tetramerization domain peptides derived from various species (2008)
  • Author(s): T. Nomura
  • Organizer: 17th Meeting of Methods in Protein Structure Analysis
  • Place of Presentation: 北海道大学学術交流会館・札幌
• [Presentation] Novel chemical inhibitors for p53-inducible protein phosphatase PPM1D (2008)
  • Author(s): H. Yagi
  • Organizer: 17th Meeting of Methods in Protein Structure Analysis
  • Place of Presentation: 北海道大学学術交流会館・札幌
• [Presentation] Thermal Stability of p53 Tetramerization Domain Peptides Derived from Various Species (2008)
  • Author(s): 野村尚生
  • Organizer: 第45回ペプチド討論会
  • Place of Presentation: タワーホール船堀・東京
• [Presentation] Identification of novel chemical inhibitors for p53-inducible protein phosphatase PPM1D (2008)
  • Author(s): 八木寛陽
  • Organizer: 第45回ペプチド討論会
  • Place of Presentation: タワーホール船堀・東京
• [Presentation] Structure Control of Amyloid Peptide Nanofibrils (2008)
  • Author(s): K. Sakaguchi
  • Organizer: The 10th RIES-Hokudai International Symposium
  • Place of Presentation: 北海道大学学術交流会館・札幌
• [Presentation] Stability of oligomeric structure is the key factor for transcriptional activity in tumor suppressor protein p53 BMB2007 (2007)
  • Author(s): T. Imagawa, et. al.
  • Organizer: 第30回日本分子生物学会年会・第80回日本生化学会大会 合同大会
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-11
• [Presentation] Correlation of oligomeric Structure and Transcritional Activity of p53 Mutants (2007)
  • Author(s): 坂口和靖, 他
  • Organizer: 第44回ペプチド討論会
  • Place of Presentation: 富山
  • Year and Date: 2007-11-07
• [Presentation] Structural photomodulation of peptides with azobenzoate derivative in peptide backbone (2007)
  • Author(s): R. Kamada, et. al.
  • Organizer: 第44回ペプチド討論会
  • Place of Presentation: 富山
  • Year and Date: 2007-11-07
• [Presentation] Correlation between expression level in E.coli and stability of oligomeric structure of the p53 tetramerization domain (2007)
  • Author(s): H. Miyazaki, et. al.
  • Organizer: 第44回ペプチド討論会
  • Place of Presentation: 富山
  • Year and Date: 2007-11-07
• [Presentation] Screening of peptides to stabilize the p53 tetramer formation from phage displayed library formation from phage displayed library (2007)
  • Author(s): T. Nomura, et. al.
  • Organizer: 第44回ペプチド討論会
  • Place of Presentation: 富山
  • Year and Date: 2007-11-07
• [Presentation] 癌抑制タンパク質p53四量体形成ドメイン (2007)
  • Author(s): 坂口和靖
  • Organizer: 日本バイオインフォマティクス学会北海道地域部会
  • Place of Presentation: 札幌
  • Year and Date: 2007-08-03
• [Presentation] Effects of Tumor-Associated Mutations in the p53 Tetramerization Domain on Oligomerization State and Transcriptional Activity (2007)
  • Author(s): R. Kamada, et. al.
  • Organizer: 20th American Peptide Society Symposium
  • Place of Presentation: Montreal, Quebec, Canada
  • Year and Date: 2007-06-26
• [Presentation] ヒト悪性腫瘍で見られる変異が癌抑制タンパク質p53四量体構造の安定性に与える影響 (2007)
  • Author(s): 鎌田瑠泉, 他
  • Organizer: 日本化学会第87春季年会
  • Place of Presentation: 大阪
  • Year and Date: 2007-03-25
• [Presentation] Correlation of Oligomeric Structure and Transcriptional Activity of p53 Mutants (2007)
  • Author(s): 坂口 和靖
  • Organizer: 第44回 ペプチド討論会
  • Place of Presentation: 富山国際会議場(富山)
• [Presentation] Effects of Tumor-Associated Mutations in the p53 Tetramerization Domain on Oligomerization State and Transcriptional Activity (2007)
  • Author(s): 鎌田瑠泉
  • Organizer: 20th American Peptide Society Symposium
  • Place of Presentation: Montreal, Canada
• [Presentation] Screening of Peptides to Stabilize the p53 Tetramer Formation from Phage Displayed Library (2007)
  • Author(s): 野村 尚生
  • Organizer: 第44回 ペプチド討論会
  • Place of Presentation: 富山国際会議場(富山)
• [Presentation] Structural photomodulation of peptides with azobenzoate derivative in peptide backbone (2007)
  • Author(s): 鎌田 瑠泉
  • Organizer: 第44回 ペプチド討論会
  • Place of Presentation: 富山国際会議場(富山)
• [Presentation] Correlation between expression level in E. coli and stability of oligomeric structure of the p53 tetramerization domain (2007)
  • Author(s): 宮崎 広充
  • Organizer: 第44回 ペプチド討論会
  • Place of Presentation: 富山国際会議場(富山)
• [Presentation] Stability of oligomeric structure is the key factor for transcriptional activity in tumor suppressor protein p53 (2007)
  • Author(s): 今川 敏明
  • Organizer: BMB2007(第30回日本分子生物学会年会 第80回日本生化学会大会 合同大会)
  • Place of Presentation: パシフィコ横浜(神奈川)
• [Presentation] Substrate Preference of p53-inducible Protein Phosphatase PPM1D (2007)
  • Author(s): 八木寛陽
  • Organizer: 4th International Peptide Symposium
  • Place of Presentation: Cairns, Australia
• [Presentation] Oligomeric Structures and Stability of Mutant p53 Tetramerization Domain Peptides Associated with Human Tumors (2006)
  • Author(s): R. Kamada, et.al.
  • Organizer: Japan - China Joint Symposium onModern Chemistry 2nd Hokkaido University-Nanjing University joint Symposium
  • Place of Presentation: Sapporo
  • Year and Date: 2006-11-10
• [Presentation] Polyol Osmolytes Affect Stability and Oligomeric State of p53 Tetramerization Domain (2006)
  • Author(s): T. Nomura, et.al.
  • Organizer: Japan-China Joint Symposium on Modern Chemistry 2nd Hokkaido University- Nanjing University joint Symposium
  • Place of Presentation: Sapporo
  • Year and Date: 2006-11-10
• [Presentation] Stabilization of p53 Tetrameric Structure by Polyol Compounds (2006)
  • Author(s): T. Nomura, et.al.
  • Organizer: 第43回ペプチド討論会
  • Place of Presentation: 横浜
  • Year and Date: 2006-11-05
• [Presentation] Thermodynamic stability of mutant p53 tetramerization domain peptides associated with human tumors (2006)
  • Author(s): R. Kamada, et.al.
  • Organizer: 第43回ペプチド討論会
  • Place of Presentation: 横浜
  • Year and Date: 2006-11-05
• [Presentation] Stabilization of p53 Tetramerization Domain by Occupying Cavities with Cyclohexylalanine (2006)
  • Author(s): T. Nomura, et. al.
  • Organizer: The 20th symposium of the protein society
  • Place of Presentation: San Diego, California, USA
  • Year and Date: 2006-08-05
• [Presentation] Amyloid formation of mutant p53 peptide was affected by hetero-oligomerization with the wild-type peptide (2006)
  • Author(s): Y. Asanomi, et.al.
  • Organizer: The 20th symposium of the protein society
  • Place of Presentation: San Diego, California, USA
  • Year and Date: 2006-08-05
• [Presentation] Effects of Mutation in p53 tetramerization domain on oligomeric structure and stability (2006)
  • Author(s): R. Kamada, et. al.
  • Organizer: 20th IUBMB International Congress of Biochemistry and Molecular Biology and 11th FAOBMB Congress
  • Place of Presentation: Kyoto
  • Year and Date: 2006-06-18
• [Presentation] Stabilization by substitution of cyclohexylalanine for phe341 in hydrophobic core of p53 tetramer (2006)
  • Author(s): T. Nomura, et. al.
  • Organizer: 20th IUBMB International Congress of Biochemistry and Molecular Biology and 11th FAOBMB Congress
  • Place of Presentation: Kyoto
  • Year and Date: 2006-06-18
• [Presentation] Correlation Between Stability of Oligomeric Structure and Transcriptional Activity in Tumor Suppressor Protein p53 Mutants (2006)
  • Author(s): T. Terai, et. al.
  • Organizer: 20th IUBMB International Congress of Biochemistry and Molecular Biology and 11th FAOBMB Congress
  • Place of Presentation: Kyoto
  • Year and Date: 2006-06-18
• [Remarks]
  • URL: http://barato.sci.hokudai.ac.jp/~biochem/index.html
• [Patent(Industrial Property Rights)] プロテインホスファターゼ阻害剤 (2008)
  • Inventor(s): 坂口和靖, 谷野圭持, 中馬吉郎, 吉村文彦, 八木寛陽
  • Industrial Property Number: 2008-262646
  • Filing Date: 2008-10-09
• [Patent(Industrial Property Rights)] p53翻訳後修飾部位を特異的に認識するーナル抗体、及びその抗体を含む修飾部位測定キット (2006)
  • Inventor(s): 坂口和靖, 中馬吉郎, 明日山康生, 松木園美穂
  • Industrial Property Number: 2007-511193
  • Filing Date: 2006-03-31