Mechanical memory effect of cells in colony

• Project/Area Number: 18340122
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Biophysics/Chemical physics
• Research Institution: Hokkaido University
• Principal Investigator: KAWABATA Kazushige Hokkaido University, 大学院・理学研究院, 教授 (20261274)
• Co-Investigator(Kenkyū-buntansha): HAGA Hisashi 北海道大学, 大学院・理学研究院, 准教授 (00292045) ; NEMOTO Koji 北海道大学, 大学院・理学研究院, 准教授 (60202248)
• Project Period (FY): 2006 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥15,940,000 (Direct Cost: ¥13,900,000、Indirect Cost: ¥2,040,000); Fiscal Year 2008: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2007: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2006: ¥7,100,000 (Direct Cost: ¥7,100,000)
• Keywords: 細胞 / 細胞コロニー / 走査型プローブ顕微鏡 / 力学的応答 / 力学的メモリー効果

Research Abstract

細胞集団がどのように連携・協同して血管や臓器などのマクロな形態を形成するかその機構を解明することを目的に、細胞に蓄積される力学的記憶効果を調べた。本研究では、走査型プローブ顕微鏡を用いた新たな測定装置を構築し、細胞に力学的変形刺激を加えた場合に細胞内に発生する力の応答を調べた。その結果、細胞の変形パターンによって、その後の細胞の力学的応答が大きく異なり、細胞レベルに力学的な記憶効果があることを明らかにした。

Research Products

• [Journal Article] Regulation of Cellular Contractile Force in Response t Mechanical Stretch by Diphosphorylation of Myosin Regulatory Light Chain via RhoA Signaling Cascade (2009)
  • Author(s): T. Mizutani, K.Kawabata, Y. Koyama, M. Takahashi, H. Haga
  • Journal Title: Cell Motility and the Cytoskeleton (in press)
  • Peer Reviewed
• [Journal Article] Mechanical Response of Sinle Myoblasts to Various Stretching Patterns Visualized by Scanning Proe Microscoy (2009)
  • Author(s): W. Mitsui, K. Tamura, T. Mizutani,Haga and K. Kawabata
  • Journal Title: Archivesof Histology and Cytology (in press)
  • Peer Reviewed
• [Journal Article] Novel Function of Transcripion Factor ATF5 : Blockade of p53-dependent Apoptosis Induced by Ionizing Irradation (2009)
  • Author(s): T. Ni shioka, Y. Miyai, H. Haga, K. Kawabata, H. Shirato, A. Homma, K. Shibata and M. Yasuda
  • Journal Title: Cell Structre and Function 34 Pages: 17-22
  • Peer Reviewed
• [Journal Article] Mechanical Response of Single Myoblasts to Various Stretching Patterns Visualized by Scanning Probe Microscopy (2009)
  • Author(s): W. Mitsui
  • Journal Title: Archives of Histology and Cytology (in press)
  • Peer Reviewed
• [Journal Article] Mechanical Response to Isotropic Shrinkage of Fibroblasts Measured by canning Probe Microscopy (2008)
  • Author(s): T. Kawamoto, H. Haga, K. Tamura, T.Mizutani and K. Kawabata
  • Journal Title: Japanese Journal of Applied Physics 47 Pages: 6173-6176
  • Peer Reviewed
• [Journal Article] Mechanical Response to Isotropic Shrinkage of Fibroblasts Measured by Scanning Probe Microscopy (2008)
  • Author(s): T. Kawamoto
  • Journal Title: Japanese Journal of Applied Physics 47 Pages: 6173-6176
  • Peer Reviewed
• [Journal Article] Visualization of stretch-induc intracellular teal response of single fibroblasts by mechanical scaning probe microscopy (2007)
  • Author(s): K. Tamura, T. Mizutani, H. Haga, K.Kawabata
  • Journal Title: Japanese Journal of Applied Physics 46 Pages: 5631-5635
  • Peer Reviewed
• [Journal Article] Imaging Mechanical Properties of Living Cells by Scanning Probe Microscoy (2007)
  • Author(s): H. Haga, M. Nagayama and K. Kawabata
  • Journal Title: Crent Nanoscien 3 Pages: 97-103
  • Peer Reviewed
• [Journal Article] Development of a Device to Stretch Tissue-like Materials and to Measure Their Mechanical Properties by Scanning Probe Microscopy (2007)
  • Author(s): T. Mizutani, H. Haga, K. Kawabta
  • Journal Title: Acta Biomaterialia 3 Pages: 485-493
  • NAID: 120000971009
  • Peer Reviewed
• [Journal Article] Collective Movement and Morphogenesis of Epithelial Cells (2007)
  • Author(s): H. Haga and K. Kawabata
  • Journal Title: Proceedings of the international symposium on topological aspects of critical systems and netwrks Pages: 82-85
  • Peer Reviewed
• [Journal Article] Visualization of stretch-induced intracellular tensional response of single fibroblasts by mechanical scanning probe microscopy (2007)
  • Author(s): K. Tamura
  • Journal Title: Japanese Journal of Applied Physics 46 Pages: 5631-5635
  • Peer Reviewed
• [Journal Article] Imaging Mechanical Properties of Living Cells by Scanning Probe Microscopy (2007)
  • Author(s): H. Haga
  • Journal Title: Current Nanoscience 3 Pages: 97-103
  • Peer Reviewed
• [Journal Article] Development of a Device to Stretch Tissue-like Materials and to Measure Their Mechanical Properties by Scanning Probe Microscopy (2007)
  • Author(s): T. Mizutani
  • Journal Title: Acta Biomaterialia 3 Pages: 485-493
  • Peer Reviewed
• [Journal Article] Collective Movement and Morphogenesis of Epithelial Cells (2007)
  • Author(s): H. Haga
  • Journal Title: Proceedings of the international symposium on topological aspects of critical systems and networks Pages: 82-85
  • Peer Reviewed
• [Journal Article] Diphosphorylation of the Myosin Regulatory Light Chain Enhancs the Tension Acting on Stress Fibers in Fibroblasts (2006)
  • Author(s): T. Mizutani, H. Haga. Y. Koyama, M.Takahashi, K. Kawabata
  • Journal Title: Journal of Cellular Physiology 209 Pages: 726-731
  • Peer Reviewed
• [Journal Article] Observation of Stiff Domain Structure on Collagen Gels by Wide-Range Scanning Probe Microscopy (2006)
  • Author(s): T. Mizutani, H. Haga, K. Kato, K. Matsuda, and K. Kawabata
  • Journal Title: Japanese Journal of Applied Physics 45 Pages: 2353-2356
  • Peer Reviewed
• [Journal Article] The Role of Actin-Binding Protein Filamin A in Cellular Stiffness and Morphology Studied by Wide-Range Scanning Probe Microscopy (2006)
  • Author(s): K. Kato, Y. Ohmori, T. Mizutani, H. Haga Kohashi, T. Ito, and K. Kawabata
  • Journal Title: Japanese Journal of Applied Physics 45 Pages: 2328-2332
  • Peer Reviewed
• [Presentation] 細胞および細胞集団の協調動における動力学的効果 (2009)
  • Author(s): 川端和重
  • Organizer: 日本機械学会第21回バイオエンジニアリング講演会基調講演
  • Place of Presentation: 札幌市
  • Year and Date: 2009-01-24
• [Presentation] 細胞および細胞集団の協調運動における動力学的効果 (2009)
  • Author(s): 川端和重
  • Organizer: 日本機械学会第21回バイオエンジニアリング講演会基調講演
  • Place of Presentation: 札幌市
  • Year and Date: 2009-01-24
• [Presentation] 細胞協同運動における細胞内および細胞間の力学的コミュニケーション (2008)
  • Author(s): 川端和重
  • Organizer: 日本生物物理学会第46回年会シンポジュームオーガナイザー
  • Place of Presentation: 福岡市
  • Year and Date: 2008-12-03
• [Presentation] バイオ高速SPMによる細胞骨格動態観察 (2008)
  • Author(s): 川端和重
  • Organizer: 日本顕微鏡学会SPM研究部会招待講演
  • Place of Presentation: 南魚沼郡湯沢町
  • Year and Date: 2008-11-28
• [Presentation] バイオ高速SPMによる細胞骨格動態観察 (2008)
  • Author(s): 川端和重
  • Organizer: 日本顕微鏡学会SPM研究部会招待講演
  • Place of Presentation: 湯沢町
  • Year and Date: 2008-11-28
• [Presentation] 高分子ゲルにおける不均一な網目構造の静止摩擦力への効果 (2008)
  • Author(s): 川端和重
  • Organizer: 摩擦の科学シンポジュームオーガナイザー
  • Place of Presentation: 刈谷市
  • Year and Date: 2008-09-11
• [Presentation] SPMによる細胞システムの協同現象の動力学的観察 (2008)
  • Author(s): 川端和重
  • Organizer: 新世代研究所バイオSPM研究会招待講演
  • Place of Presentation: 新潟市
  • Year and Date: 2008-03-14
• [Presentation] SPMによる細胞システムの協同現象の動力学的観察 (2008)
  • Author(s): 川端 和重
  • Organizer: 新世代研究所バイオSPM研究会
  • Place of Presentation: 新潟
  • Year and Date: 2008-03-14
• [Presentation] 細胞システムの協同現象における動力学効果 (2007)
  • Author(s): 川端和重
  • Organizer: 日本生物物理学会シンポジューム招待講演
  • Place of Presentation: 横浜市
  • Year and Date: 2007-12-23
• [Presentation] 細胞システムの協同現象における動力学効果 (2007)
  • Author(s): 川端 和重
  • Organizer: 日本生物物理学会シンポジューム
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-23
• [Presentation] 細胞系における協調運動に伴う局所弾性率の時空間測定 (2007)
  • Author(s): 芳賀永
  • Organizer: 日本表面科学会第27回表面科学講演大会招待講演
  • Place of Presentation: 東京都
  • Year and Date: 2007-11-01
• [Presentation] 細胞系における協調運動に伴う局所弾性率の時空間測定 (2007)
  • Author(s): 芳賀 永
  • Organizer: 日本表面科学会 第27回表面科学講演大会
  • Place of Presentation: 東京
  • Year and Date: 2007-11-01
• [Presentation] Wide range scanning probe microscopy for probing mechanical properties i a sine cell orggregative cells (2007)
  • Author(s): T. Mizutani
  • Organizer: Advanced Technology Institute International Forum 2007招待講演
  • Place of Presentation: 千葉市
  • Year and Date: 2007-10-22
• [Presentation] Wide range scanning probe microscopy for probing mechanical properties in a single cell or aggregative cells (2007)
  • Author(s): T. Mizutani
  • Organizer: Advanced Technology Institute International Forum 2007
  • Place of Presentation: 千葉
  • Year and Date: 2007-10-22
• [Presentation] 走査型プローブ顕微鏡による細胞集団の硬さ分布測定と細胞運動の観察 (2007)
  • Author(s): 水谷武臣
  • Organizer: 日本顕微鏡学会第63回学術講演会招待講演
  • Place of Presentation: 新潟市
  • Year and Date: 2007-05-20
• [Presentation] 走査型プローブ顕微鏡による細胞集団の硬さ分布測定と細胞運動の観察 (2007)
  • Author(s): 水谷 武臣
  • Organizer: 日本顕微鏡学会第63回学術講演会
  • Place of Presentation: 新潟
  • Year and Date: 2007-05-20
• [Presentation] 染色体凝集解明の力学的アプローチ (2007)
  • Author(s): 川端和重
  • Organizer: 日本応用物理学会シンポジウム招待講演
  • Place of Presentation: 相模原市
  • Year and Date: 2007-03-28
• [Presentation] ゲル界面の摩擦の素過程と制御 (2007)
  • Author(s): 川端和重
  • Organizer: 日本物理学会シンポジウム招待講演
  • Place of Presentation: 鹿児島市
  • Year and Date: 2007-03-18
• [Presentation] 細胞の力学量マッピングと運動制御 (2006)
  • Author(s): 川端和重
  • Organizer: 新世代研究所合同シンポジウム招待講演
  • Place of Presentation: 長野市
  • Year and Date: 2006-11-10
• [Presentation] 生細胞の力学的イメージングとその運動制御 (2006)
  • Author(s): 川端和重
  • Organizer: 学術振興会ナノプローブテクノロジー第167委員会「光・プローブ技術を駆使した細胞操作とイメージング」招待講演
  • Place of Presentation: 横浜市
  • Year and Date: 2006-10-26
• [Presentation] Stiffness Measurement of an Epithelial Colony Using Wide-Range Scanning Probe Microscopy (2006)
  • Author(s): T. Mizutani
  • Organizer: The 16th International Microscopy Congress招待講演
  • Place of Presentation: 札幌市
  • Year and Date: 2006-09-07
• [Presentation] Stiffness Response to External Deformation of Single Cells and Colony (2006)
  • Author(s): K. Kawabata
  • Organizer: The 3rd Conference on Artificial Muscles Collaboration with Nanotechnology and Biology招待講演
  • Place of Presentation: 東京都
  • Year and Date: 2006-05-31
• [Presentation] 単一生細胞の力学的履歴効果 (2006)
  • Author(s): 川端和重
  • Organizer: 第45回日本生体医工学会大会 招待講演
  • Place of Presentation: 福岡市
  • Year and Date: 2006-05-15
• [Book] Applied Scanning Probe Methods X (2008)
  • Author(s): T. Ushiki and K. Kawabata
  • Publisher: Springer-Verlag
• [Book] Chromosome Nanoscience and Technology (2007)
  • Author(s): K. Kawabata, K. Nomura, K. Ikeda. O. Hoshi, D. Fukushi, H. Haga and T. Ushiki
  • Publisher: Taylor & Francis Group
• [Remarks]
  • URL: http://altair.sci.hokudai.ac.jp/g3/researchj.html
• [Remarks]
  • URL: http://altair.sci.hokudai.ac.jp/g3/researchj.html
• [Remarks]
  • URL: http://altair.sci.hokudai.ac.jp/g3/researchj.html