Study of the methods for visualizing intracellular signaling molecules
| Project/Area Number |
18350045
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Analytical chemistry
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| Research Institution | The University of Tokyo (2007-2008)
Institute for Molecular Science (2006) |
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Principal Investigator |
OZAWA Takeaki The University of Tokyo, 大学院・理学系研究科, 教授 (40302806)
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| Co-Investigator(Kenkyū-buntansha) |
TAKEUCHI Masaki 東京大学, 大学院・理学系研究科, 助教 (00332271)
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|---|
| Project Period (FY) |
2006 – 2008
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| Project Status |
Completed (Fiscal Year 2008)
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| Budget Amount *help |
¥16,880,000 (Direct Cost: ¥14,600,000、Indirect Cost: ¥2,280,000)
Fiscal Year 2008: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2007: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2006: ¥7,000,000 (Direct Cost: ¥7,000,000)
|
|---|
| Keywords | 可視化 / バイオテクノロジー / 分析化学 / 生体分子 |
|---|
| Research Abstract |
In the present study, we studied the development of optical probes for visualizing bio molecules using luciferases, GFP and its spectral variants based on protein reconstitution methods. Moreover, we generated new concepts for molecular imaging such as luciferase cyclization by protein splicing and spatiotemporal analysis of protein-protein interactions based on split luciferase complementation. These results are expected to contribute to resolving biological function in live cells.
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Report
(4 results)
Research Products
(75 results)
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[Book] 光る遺伝子2009
Author(s)
小澤岳昌(監訳), 大森充香(訳)
Total Pages
272
Publisher
丸善
Related Report
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