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Application of a dsDNA imprinted polymer to detection for bacterial toxin

Research Project

Project/Area Number 18350080
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Environmental chemistry
Research InstitutionTokyo University of Technology

Principal Investigator

MINOURA Norihiko  Tokyo University of Technology, School of Bionics, 教授 (10358111)

Co-Investigator(Kenkyū-buntansha) SHINBO Toshio  National Institute of Advanced Industrial Science and Technology, Research Center of Advanced Bionics, 副センター長 (70357250)
Project Period (FY) 2006 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥17,420,000 (Direct Cost: ¥15,200,000、Indirect Cost: ¥2,220,000)
Fiscal Year 2007: ¥9,620,000 (Direct Cost: ¥7,400,000、Indirect Cost: ¥2,220,000)
Fiscal Year 2006: ¥7,800,000 (Direct Cost: ¥7,800,000)
KeywordsMolecular imprinting / Polymer gel / Electrophoresis / dsDNA / DNA sequence / Molecular recognition / Template / Verotoxin / 分子認織 / ペロ毒素
Research Abstract

Molecularly imprinted polymers(MIPs) are materials that are attractive for research applications because they have an artificial recognition cavity for the target molecule. We prepared a MIP for recognizing a specific dsDNA sequence by using 2-vinyl-4, 6-diamino-1, 3, 5-triazine(VDAT) as a functional monomer and used it in an electrophoretic gel matrix.
Firstly, interaction between VDAT analog as a new functional monomer and dsDNA was investigated to improve the specificity of target dsDNA to MIP gel. From the result, some candidate compounds were selected as new functional monomer and application of these compounds to MIP is now in progress.
To achieve a miniaturization of detection device, a label-free sample, and a real time detection, we demonstrate a novel dsDNA detection system with dsDNA-imprinted polymer gel by using VDAT as a functional monomer. The miniature-sized gel matrix was used in this system. The dsDNA eluted through the MIP gel during electrophoresis was stained with fluorescence molecule at an edge of the gel and was carried to fluorescence detector by carrier solution. In this system, elution time of dsDNA with the target sequence should be elongated in comparison with the non-imprinted polymer gel by the capture effect of the binding sites in the MIP gel. Under optimum measurement condition, the elongation of elution time on λDNA 564bp fragment was confirmed in a λDNA 564bp fragment-imprinted polymer gel.
Application of this system to a bacterial dsDNA detection was also investigated. However, the target bacterial dsDNA was not detected. The DNA size of bacterial dsDNA(34bp) is smaller in comparison with the λDNA 564bp fragment. It suggests that there is an influence of size of DNA on detection of target dsDNA, since the number of functional groups surrounding the binding sites in MIP gel increases with increasing the size of target dsDNA. We are planning to study the relationship between size of target dsDNA and detection limit in this system.

Report

(3 results)
  • 2007 Annual Research Report   Final Research Report Summary
  • 2006 Annual Research Report
  • Research Products

    (26 results)

All 2008 2007 2006 2005

All Journal Article (8 results) (of which Peer Reviewed: 3 results) Presentation (13 results) Book (3 results) Patent(Industrial Property Rights) (2 results)

  • [Journal Article] Preparation of gels with a molecular recognition function by molecular imprinting technology.2008

    • Author(s)
      Norihiko Minoura(Ryo Yoshida, Ed.)
    • Journal Title

      The Latest Technology and Development of Medical Gels, CMG Books

      Pages: 70-82

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] DNA detection system using molecularly imprinted polymer as the gel matrix in electrophoresis2007

    • Author(s)
      Masayo Ogiso
    • Journal Title

      Biosensors & Bioelectronics 22

      Pages: 1974-1981

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] DNA detection system using molecularly imprinted polymer as the gel matrix in electrophoresis.2007

    • Author(s)
      Masayo Ogiso, Norihiko Minoura, Toshio Shinbo, Toshimi Shimizu.
    • Journal Title

      Biosensors & Bioelectronics 22

      Pages: 1974-1981

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Toxin sensors.2007

    • Author(s)
      Norihiko Minoura(Isao Karube, Ed.)
    • Journal Title

      Handbook of Biosensors and Chemical Sensors, Technosystem

      Pages: 366-373

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] DNA detection system using molecularly imprinted polymer as the gelmatrix in electrophoresis2007

    • Author(s)
      Masayo Ogiso
    • Journal Title

      Biosensors & Bioelectronics 22

      Pages: 1974-1981

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Detection of a specific DNA sequence by electrophoresis through a molecularly imprinted polymer2006

    • Author(s)
      Masayo Ogiso
    • Journal Title

      Biomaterials 27

      Pages: 4177-4182

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Detection of a specific DNA sequence by electrophoresis through a molecularly imprinted polymer.2006

    • Author(s)
      Masayo Ogiso, Norihiko Minoura, Toshio Shinbo, Toshimi Shimizu.
    • Journal Title

      Biomaterials 27

      Pages: 4177-4182

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Detection of a specific DNA sequence by electrophoresis through a molecularly imprinted polymer2006

    • Author(s)
      M.Ogiso, et al.
    • Journal Title

      Biomaterials 27

      Pages: 4177-4182

    • Related Report
      2006 Annual Research Report
  • [Presentation] 分子認識部位を有するポリマーゲルを用いたDNA検出システムの開発2007

    • Author(s)
      小木曽 真佐代
    • Organizer
      第7回界面ナノアーキテクトニクスワークショップ
    • Place of Presentation
      産業技術総合研究所つくば中央
    • Year and Date
      2007-12-14
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Development of DNA deteption system by using a molecularly imprinted polymer gel with DNA recognition sites.2007

    • Author(s)
      Masayo Ogiso, Norihiko Minoura, Toshio Shinbo.
    • Organizer
      7th Nanoarchitectonics Workshop
    • Place of Presentation
      National Institute of Advanced Industrial Science
    • Year and Date
      2007-12-14
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] DNA detection system using molecularly imprinted polymer as the gel matrix in electrophoresis2007

    • Author(s)
      Masayo Ogiso
    • Organizer
      1st Asian Biomaterials Congress
    • Place of Presentation
      Epochal Tsukuba International Congress Center
    • Year and Date
      2007-12-07
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] DNA detection system using molecularly imprinted polymer as the gel matrix in electrophoresis.2007

    • Author(s)
      Masayo Ogiso, Norihiko Minoura, Toshio Shinbo.
    • Organizer
      1st Asian Biomaterials Congress.
    • Place of Presentation
      Epochal Tsukuba International Congress Center
    • Year and Date
      2007-12-07
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] DNA detection system using molecularly imprinted polymer as the gelmatrix in electrophoresis2007

    • Author(s)
      M. Ogiso
    • Organizer
      1st Asian Biomaterials Congress
    • Place of Presentation
      Epochal Tsukuba International Congress Center
    • Year and Date
      2007-12-07
    • Related Report
      2007 Annual Research Report
  • [Presentation] 分子認識部位を有するポリマーゲルを用いた電気泳動法による生体分子検出方法の開発2007

    • Author(s)
      小木曽 真佐代
    • Organizer
      日本化学会第87春季年会
    • Place of Presentation
      関西大学千里山キャンパス
    • Year and Date
      2007-03-26
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Development of a biomolecule detection system by using a molecularly imprinted polymer gel.2007

    • Author(s)
      Norihiko Minoura, Masayo Ogiso, Toshio Shinbo.
    • Organizer
      87th The Chemical Society of Japan
    • Place of Presentation
      Kansai University
    • Year and Date
      2007-03-26
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 分子インプリンティングポリマーを用いたDNA検出方法の開発2006

    • Author(s)
      箕浦 憲彦
    • Organizer
      日本膜学会第28年会
    • Place of Presentation
      北海道大学学術交流会館
    • Year and Date
      2006-06-08
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Detection of double-stranded dsDNA by using a molecularly imprinted polymer gel.2006

    • Author(s)
      Norihiko Minoura, Masayo Ogiso, Toshio Shinbo, Toshimi Shimizu.
    • Organizer
      28th The Membrane Society of Japan
    • Place of Presentation
      Hokkaido University
    • Year and Date
      2006-06-08
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 分子認識ゲル媒体を用いた電気泳動法による塩基配列特異的DNAの検出2006

    • Author(s)
      小木曽 真佐代
    • Organizer
      第55回高分子学会年次大会
    • Place of Presentation
      名古屋国際会議場
    • Year and Date
      2006-05-25
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Detection of a target dsDNA by electrophoresis through a molecularly imprinted gel matrix2006

    • Author(s)
      Norihiko Minoura, Masavo Ogiso, Toshio Shinbo, Toshimi Shimizu.
    • Organizer
      55th The Society of Polymer Science, 25
    • Place of Presentation
      Nagoya Congress Center
    • Year and Date
      2006-05-25
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] DNA detection system using molecularly imprinted polymer as a gel matrix of electrophoresis2006

    • Author(s)
      Norihiko Minoura
    • Organizer
      Biosensors 2006
    • Place of Presentation
      Toronto Canada
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] DNA detection system using molecularly imprinted polymer as a gel matrix of electrophoresis.2006

    • Author(s)
      Norihiko Minoura, Masayo Ogiso, Toshio Shinbo, Toshimi Shimizu.
    • Organizer
      Biosensors 2006
    • Place of Presentation
      Tronto, Canada.
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Book] 分子インプリンティングによる分子認識機能ゲルの創製(医療用ゲルの最新技術と開発)2008

    • Author(s)
      箕浦 憲彦(監修:吉田亮)
    • Publisher
      シーエムシー出版
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Book] 毒物バイオセンサ(バイオセンサ・ケミカルセンサ事典)2007

    • Author(s)
      箕浦 憲彦(監修:軽部征夫)
    • Publisher
      (株)テクノシステム
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Book] 毒物バイオセンサ(バイオセンサ・ケミカルセンサ事典)2007

    • Author(s)
      箕浦憲彦(監修:軽部征夫)
    • Publisher
      (株)テクノシステム
    • Related Report
      2007 Annual Research Report
  • [Patent(Industrial Property Rights)] DNA分子識別機能材料2007

    • Inventor(s)
      箕浦憲彦・小木曽真佐代
    • Industrial Property Rights Holder
      (独)産業技術総合研究所
    • Acquisition Date
      2007-09-02
    • Related Report
      2007 Annual Research Report
  • [Patent(Industrial Property Rights)] 標的DNA解析方法及び解析装置2005

    • Inventor(s)
      箕浦憲彦・小木曽真佐代・新保外志夫
    • Industrial Property Rights Holder
      (独)産業技術総合研究所
    • Filing Date
      2005-09-27
    • Related Report
      2007 Annual Research Report

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Published: 2006-04-01   Modified: 2016-04-21  

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