Chemical and biochemical studies on the relationship between NO-induced oxanine formation as gene dmage and its cancer generation

• Project/Area Number: 18350083
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Chemistry related to living body
• Research Institution: Kyoto University
• Principal Investigator: MAKINO Keisuke Kyoto University, エネルギー理工学研究所, 教授 (50159141)
• Co-Investigator(Kenkyū-buntansha): KODAKI Tsutomu 京都大学, エネルギー理工学研究所, 准教授 (70170264) ; KANAORI Kenji 京都工芸繊維大学, 大学院・工芸科学研究科, 准教授 (30273543)
• Project Period (FY): 2006 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥17,770,000 (Direct Cost: ¥15,400,000、Indirect Cost: ¥2,370,000); Fiscal Year 2008: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2007: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2006: ¥7,500,000 (Direct Cost: ¥7,500,000)
• Keywords: 一酸化窒素 / 損傷塩基 / オキザニン / 架橋形成 / 発ガン / 修復酵素 / DNA認識酵素 / 修復機構存在

Research Abstract

一酸化窒素によってDNA中のグアニン(Gua)から生成するオキザニン(Oxa)と発ガンとの関係を検討した。Oxaは遺伝子の修飾を司る酵素(制限酵素、キナーゼ、リガーゼ等)によってGuaと認識され、認識中に架橋反応することはないが、 DNA修復酵素等とは架橋反応を引き起こす。これらの知見と化学合成法を組み合わせてタンパク質-DNA架橋モデルを合成し、この損傷への修復機能の存在を明らかにし、発ガンとの関係が否定できないことを明らかにした。

Research Products

• [Journal Article] Reinvestigation of the molecular influence of hypoxanthine on the DNA cleavage efficiency of restriction endonucleases BglII, EcoRI and BamHI (2008)
  • Author(s): A. Doi, S. P. Pack, T. Kodaki, and K. Makino
  • Journal Title: J. Biochem Accepted
  • NAID: 10026302695
  • Peer Reviewed
• [Journal Article] Ozone production by amino acids contributes to killing of bacteria (2008)
  • Author(s): K. Yamashita, T. Miyoshi, T. Arai, N. Endo, H. Itoh, K. Makino, K. Mizugishi, T. Uchiyama, and M. Sasada
  • Journal Title: Proc. Natl. Acad. Sci. U.S.A. 105 Pages: 16912-16971
  • Peer Reviewed
• [Journal Article] Metabolic fate of l-lactaldehyde derived from an alternative l-rhamnose pathway (2008)
  • Author(s): S. Watanabe, S. Piyanart, and K. Makino
  • Journal Title: FEBS J. 275 Pages: 5139-5149
  • Peer Reviewed
• [Journal Article] Eukaryotic and bacterial gene clusters related to an alternative pathway of nonphosphorylated L-rhamnose metabolism (2008)
  • Author(s): S. Watanabe, M. Saimura, and K. Makino
  • Journal Title: J Biol Chem. 283 Pages: 20372-20382
  • Peer Reviewed
• [Journal Article] Bioethanol Production from Xylose by Recombinant Saccharomyces cerevisiae Expressing Xylose Reductase (2008)
  • Author(s): A. Matsushika, S. Watanabe, T. Kodaki, K. Makino, S. Sawayama
  • Journal Title: J. Biosci. Bioeng. 105 Pages: 296-299
  • Peer Reviewed
• [Journal Article] The radical scavenger edaravone (3-methyl-1-phenyl-2-pyrazolin-5-one) rescts with a pterin derivative and (2008)
  • Author(s): T. Arai, M. Nonogawa, K. Makino, N. Endo, H. Mori, T. Miyoshi, K.
  • Journal Title: J. Pharmacol. Exp. Ther. 324 Pages: 529-538
  • Peer Reviewed
• [Journal Article] Anticancer photodynamic and non-photodynamic effects of pterin derivatives on a pancreatic cancer cell line. (2008)
  • Author(s): T. Miyoshi, T. Arai, M. Nonogawa, K. Makino, H. Mori, K. Yamashita
  • Journal Title: J. Pharmacol. Sci. 107 Pages: 221-225
  • NAID: 10024321141
  • Peer Reviewed
• [Journal Article] Eukaryotic and bacterial gene clusters related to an alternative pathway of nonphosphorylated L-rhamnose (2008)
  • Author(s): S. Watanade, M. Saimura, K. Makino.
  • Journal Title: J Biol Chem. 283 Pages: 20372-20382
  • Peer Reviewed
• [Journal Article] Expression of protein engineered NADP (+)-dependent xylitol dehyrogenase increases ethanol production from (2008)
  • Author(s): A. Matsushika, S. Watanabe, T. Kodaki, K. Makino, H. Inoue, K.
  • Journal Title: Appl. Microbiol. Biotechnol. 81 Pages: 243-255
  • Peer Reviewed
• [Journal Article] Metabolic fate of 1-lactaldehyde derived from analternative 1-rhamnose pathway. (2008)
  • Author(s): S. Watanabe, S. Piyanart, K. Makino.
  • Journal Title: FEBS J. 275 Pages: 5139-5149
  • Peer Reviewed
• [Journal Article] Ozone production by amino acids contributes to killing of bacteria. (2008)
  • Author(s): K. Yaraashita, T. Miyoshi, T. Aral, N. Endo, H. Itoh, K.
  • Journal Title: Proc. Natl. Acad. Sci. U.S.A. 105 Pages: 16912-16917
  • Peer Reviewed
• [Journal Article] Reinvestigation of the molecular influence of hypoxanthine on the DNA cleavage efficiency of (2008)
  • Author(s): A. Doi, S. P. Pack, T. Kodaki, K. . Makino.
  • Journal Title: J. Biochem
  • Peer Reviewed
• [Journal Article] Direct immobilization of DNA oligomers onto the amine-functionalized glass surface for DNA microarray fabrication through the activation-free reaction of oxanine (2007)
  • Author(s): S. P. Pack, N. K. Kamisetty, M. Nonogawa, K. C. Devarayapalli, K. Ohtani, K. Yamada, Y. Yoshida, T. Kodaki, and K. Makino
  • Journal Title: Nucleic Acids Res. 35
  • Peer Reviewed
• [Journal Article] Additional alkylsilanization of aminosilane-modified glass slide: effect of alkylsilane structure for enhancing surface amine functionality (2007)
  • Author(s): N. K. Kamisetty, S. P. Pack, M. Nonogawa, D. K. Charyulu, T. Kodaki and K. Makino
  • Journal Title: Chem. Lett. 36 Pages: 322-323
  • NAID: 10018523096
  • Peer Reviewed
• [Journal Article] Efficient preparation of amine-modified oligodeoxynucleotide using a modified H-phosphonate chemistry for DNA microarray fabrication (2007)
  • Author(s): N. K. Kamisetty, S. P. Pack, M. Nonogawa, D. K. Charyulu, S. Watanabe, T. Kodaki and K. Makino
  • Journal Title: Anal. Bioanal. Chem. 387 Pages: 2027-2037
  • Peer Reviewed
• [Journal Article] Base sequence- and Tm-dependent DNA oligomer separation by open tubular capillary columns carrying complementary DNA oligomers as probes (2007)
  • Author(s): K. C. Devarayapalli, S. P. Pack, N. K. Kamisetty, S. Watanabe, T. Kodaki, and K. Makino
  • Journal Title: Anal. Bioanal. Chem. 388 Pages: 919-928
  • Peer Reviewed
• [Journal Article] Ethanol production from xylose by recombinant Saccharomyces cerevisiae expressing protein engineered NADP+-dependent xylitol dehydrogenase (2007)
  • Author(s): S. Watanabe, A. A. Saleh, S. P. Pack, N. Annaluru, T. Kodaki and K. Makino
  • Journal Title: J. Biotechn. 130 Pages: 316-319
  • Peer Reviewed
• [Journal Article] Protein engineering of xylose reductase from Pichia stipitis for improved NADH-specificity and the efficient ethanol production from xylose in recombinant Saccharomyces cerevisiae (2007)
  • Author(s): Watanabe, A. A. Saleh, S. P. Pack, N. Annaluru, T. Kodaki and K. Makino
  • Journal Title: Microbiology 153 Pages: 3044-3054
  • Peer Reviewed
• [Journal Article] Nucleotide excision repair and homologous recombination systems commit differentially to the repair of DNA-protein crosslinks (2007)
  • Author(s): T. Nakano, S. Morishita. A. Katafuchi, M. Matsubara, Y. Horikawa, H. Terato, A. M. H. Salem, S. Izumi, S. P. Pack, K. Makino and H. Ide
  • Journal Title: Mol. Cell 28 Pages: 147-158
  • Peer Reviewed
• [Journal Article] Thermostabilization of Pichia stipitis xylitol dehydrogenase by mutation of structural zinc-binding loop (2007)
  • Author(s): N. Annaluru, S. Watanabe, S.P. Pack, A. A. Saleh, T. Kodaki and K. Makino
  • Journal Title: J. Biotech. 129 Pages: 717-722
  • Peer Reviewed
• [Journal Article] Biophysical stability and enzymatic recognition of oxanine in DNA (2007)
  • Author(s): S. P. Pack, A. Doi, M. Nonogawa, N. K. Kamisetty, K. C. Devarayapalli, T. Kodaki, and K. Makino
  • Journal Title: Nucleotides and Nucleic Acids 26 Pages: 1589-1593
  • Peer Reviewed
• [Journal Article] Base sequence-and T_m-dependent DNA oligomer separation by open tubular capillary columns carrying complementary DNA oligomers as probes (2007)
  • Author(s): K. C. Devarayapalli, S. P. Pack, N. K. Kamisetty, S. Watanabe, T. Kodaki, and K. Makino
  • Journal Title: Anal. Bioanal. Chem. 388 Pages: 919-928
  • Peer Reviewed
• [Journal Article] The positive effect of the decreased NADPH-preferring activity of xylose reductase from Pichia stipitis on the ethanol production using xylose-fermenting recombinant Saccharomyces cerevisiae (2007)
  • Author(s): S. Watanabe, A. A. Saleh, S. P. Pack, N. Annaluru, T. Kodaki and K. Makino
  • Journal Title: Biosci. Biotech. Biochem. 71 Pages: 1365-1369
  • NAID: 10027515216
  • Peer Reviewed
• [Journal Article] Ethanol production from xylose by recombinant Saccharomyces cerevisiae expressing protein engineered NADP^+-dependent xylitoldehydrogenase (2007)
  • Author(s): S. Watanabe, A. A. Saleh, S. P. Pack, N.Annaluru, T. Kodaki and K. Makino
  • Journal Title: J. Biotechn. 130 Pages: 316-319
  • Peer Reviewed
• [Journal Article] Protein engineering of xylose reductase from Pichia stipitis for improved NADH-specificity and the efficient ethanol production from xylose in recombinant Saccharomyces cerevisiae (2007)
  • Author(s): S. Watanabe, A. A. Saleh, S. P. Pack, N. Annaluru, T. Kodaki and K. Makino
  • Journal Title: Microbiology 153 Pages: 3044-3054
  • Peer Reviewed
• [Journal Article] Direct immobilization of DNA oligomers onto the amine-functionalized glass surface for DNA microarray fabrication through theactivation-free reaction of oxanine (2007)
  • Author(s): S. P. Pack, N. K. Kamisetty, M. Nonogawa, K. C. Devarayapalli, K. Ohtani, K. Yamada, Y. Yoshida, T. Kodaki, and K. Makino
  • Journal Title: Nucleic Acids Res. 35
  • Peer Reviewed
• [Journal Article] Nucleotide excision repair and homologous recombination systems commit differentially to the repair of DNA-protein crosslinks (2007)
  • Author(s): S. Watanabe, A. A. Saleh, S. P. Pack, N. Annaluru, T. Kodaki and K. Makino Toshiaki Nakano, Soh Morishita. Atsushi Katafuchi, Mayumi Matsubara, Yusuke Horikawa, Hiroaki Terato, Amir M.H. Salem, Shunsuke Izumi, Seung Pil Pack, Keisuke Makino and Hiroshi Ide
  • Journal Title: Molecular Cell 28 Pages: 147-158
  • Peer Reviewed
• [Journal Article] Thermostabilization of Pichia stipitis xylitol dehydrogenase bymutation of structural zinc-binding loop (2007)
  • Author(s): N. Annaluru, S. Watanabe, S. P. Pack, A. A. Saleh, T. Kodaki and K. Makino
  • Journal Title: J. Biotech. 129 Pages: 717-722
  • Peer Reviewed
• [Journal Article] Biophysical stability and enzymatic recognition of oxanine in DNA. (2007)
  • Author(s): S. P. Pack, A. Doi, M. Nonogawa, N. K. Kamisetty, K. C. Devarayapalli, T. Kodaki, and K. Makino
  • Journal Title: Nucleosides, Nucleotides, and Nucleic Acids 26 Pages: 1589-1593
  • Peer Reviewed
• [Journal Article] α-Ketoglutaric semialdehyde dehydrogenase isozymes involved in metabolic pathways of D-glucarate and hydroxy-L-proline. (2007)
  • Author(s): S.Watanabe, M.Yamada, I.Ohtsu, K.Makino
  • Journal Title: J. Biol. Chem. 282 Pages: 6685-6695
• [Journal Article] Additional alkylsilanization of aminosilane-modified glass slide : effect of alkylsilane structure for enhancing surface amine functionality. (2007)
  • Author(s): N.K.Kamisetty, S.P.Pack, M.Nonogawa, D.K.Charyulu, T.Kodaki, K.Makino
  • Journal Title: Chem. Lett. 36 Pages: 322-323
  • NAID: 10018523096
• [Journal Article] Efficient preparation of amine-modified oligodeoxynucleotide using a modified H-phosphonate chemistry for DNA microarray fabrication. (2007)
  • Author(s): N.K.Kamisetty, S.P.Pack, M.Nonogawa, D.K.Charyulu, S.Watanabe, T.Kodaki, K.Makino
  • Journal Title: Anal. Bioanal. Chem. 387 Pages: 2027-2037
• [Journal Article] Reactive oxygen species generation through NADH oxidation by 6-formylpterin derivatives in the dark. (2007)
  • Author(s): M.Nonogawa, S-P.Pack, To.Arai, N.Endo, P.Sommani, T.Kodaki, K.Makino
  • Journal Title: Biochem. Biophys. Res. Comn. 353 Pages: 1105-1110
• [Journal Article] Thermostabilization of Pichia stipitis xylitol dehydrogenase by mutation of structural zinc-binding loop. (2007)
  • Author(s): N.Annaluru, S.Watanabe, S.P.Pack, A.A.Saleh, T.Kodaki, K.Makino
  • Journal Title: J. Biotech. 129 Pages: 717-722
• [Journal Article] Development of efficient amine-functionalized glass platform by additional alkylsilane silanization treatment (2006)
  • Author(s): N. K. Kamisetty, S. P. Pack, M. Nonogawa, K. C. Devarayapalli, T. Kodaki, and K. Makino
  • Journal Title: Anal. Bioanal. Chem. 386 Pages: 1649-1656
  • Peer Reviewed
• [Journal Article] A novel α-ketoglutaric semialdehyde dehydrogenase - Evolutionary insight into an alternative pathway of bacterial L-arabinose metabolism (2006)
  • Author(s): S. Watanabe, T. Kodaki, and K. Makino
  • Journal Title: J. Biol. Chem 281 Pages: 28876-28888
  • Peer Reviewed
• [Journal Article] Gene expression in enhanced apoptosis of human lymphoma U937 cells treated with the combination of different free radical generators and hyperthermia (2006)
  • Author(s): S. Wada, Y. Tabuchi, T. Kondo, Z.-G Cui, Q.-L. Zhao, I. Takasaki, T. L. Salunga, R. Ogawa, T. Arai, K. Makino, and I. Furuta
  • Journal Title: Free Rad. Res. 41 Pages: 73-81
  • Peer Reviewed
• [Journal Article] Identification and characterization of L-arabonate dehydratase, L-2-keto-3-deoxyarabonate dehydratase and L-arabinolactonase involved in an alternative pathway of L-arabinose metabolism: novel evolutionary insight into sugar metabolism (2006)
  • Author(s): S. Watanabe, N. Shimada, K. Tajima, T. Kodaki, and K. Makino
  • Journal Title: J. Biol. Chem. 281 Pages: 33521-33536
  • Peer Reviewed
• [Journal Article] Novel 6-formylpterin derivatives: Chemical synthesis and O2 to ROS conversion activities (2006)
  • Author(s): M. Nonogawa, T. Arai, N. Endo, S.P. Pack, T. Kodaki, and K. Makino
  • Journal Title: Org. Biomol. Chem. 4 Pages: 1811-1816
  • Peer Reviewed
• [Journal Article] Structural property and enzymatic response of oxanine in DNA strands (2006)
  • Author(s): S. P. Pack, A. Doi, M. Nonogawa, N. K. Kamisetty, K. C. Devarayapalli, T. Kodaki, and K. Makino
  • Journal Title: Nucleic Acids Res. Symp. Ser. 50 Pages: 97-98
  • Peer Reviewed
• [Journal Article] α-Ketoglutaric semialdehyde dehydrogenase isozymes involved in metabolic pathways of D-glucarate and hydroxy-L-proline (2006)
  • Author(s): S. Watanabe, M. Yamada, I. Ohtsu, and K. Makino
  • Journal Title: J. Biol. Chem. 282 Pages: 6685-6695
  • Peer Reviewed
• [Journal Article] Development of efficient amine-functionalized glass platform by additional alkylsilane silanization treatment. (2006)
  • Author(s): N.K.Kamisetty, S.P.Pack, M.Nonogawa, K.C.Devarayapalli, T.Kodaki, K.Makino
  • Journal Title: Anal. Bioanal. Chem. 386 Pages: 1649-1656
• [Journal Article] A novel α-ketoglutaric semialdehyde dehydrogenase - Evolutionary insight into an alternative pathway of bacterial L-arabinose metabolism. (2006)
  • Author(s): S.Watanabe, T.Kodaki, K.Makino
  • Journal Title: J. Biol. Chem. 281 Pages: 28876-28888
• [Journal Article] Gene expression in enhanced apoptosis of human lymphoma U937 cells treated with the combination of different free radical generators and hyperthermia. (2006)
  • Author(s): S Wada, Y.Tabuchi, T.Kondo, Z.-G.Cui, Q.-L.Zhao, I.Takasaki, T.L.Salunga, R.Ogawa, T.Ara, K.Makino, I.Furuta
  • Journal Title: Free Rad. Res. 41 Pages: 73-81
• [Journal Article] Identification and characterization of L-arabonate dehydratase,L-2-keto-3-deoxyarabonate dehydratase and L-arabinolactonase involved in an alternative pathway of L-arabinose metabolism : novel evolutionary insight into sugar metabolism. (2006)
  • Author(s): S.Watanabe, N.Shimada, K.Tajima, T.Kodaki, K.Makino
  • Journal Title: J. Biol. Chem. 281 Pages: 33521-33536
• [Journal Article] Novel 6-formylpterin derivatives : Chemical synthesis and O_2 to ROS conversion activities. (2006)
  • Author(s): M.Nonogawaa, T.Arai, N.Endo, S.P.Pack, T.Kodaki, K.Makino
  • Journal Title: Org. Biomol. Chem. 4 Pages: 1811-1816
• [Journal Article] Structural property and enzymatic response of oxanine in DNA strands. (2006)
  • Author(s): S.P.Pack, A.Doi, M.Nonogawa, N.K.Kamisetty, K.C.Devarayapalli, T.Kodaki, K.Makino
  • Journal Title: Nucleic Acids Symp. Ser. 50 Pages: 97-98
• [Presentation] Investigation of interaction between acid/base catalyst amino acid residues and oxanine, a novel oxidative lesion from guanine by nitrous acid (2008)
  • Author(s): A. Doi, S.P. Pack, M. Saimura, M. Nonogawa, T. Kodaki, and K. Makino
  • Organizer: 第31回日本分子生物学会年会 第81回日本生化学会大会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-12
• [Presentation] Investigation of interaction between acid/base catalystamino acid residues and oxanine, a novel oxidative (2008)
  • Author(s): A. Doi, S. P. Pack, M. Saimura, M. Nonogawa, T. Kodaki, K. Makino
  • Organizer: 第31回日本分子生物学会年会 第81回日本生化学会大会 合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-12
• [Presentation] タンパク質工学を用いたキシロース発酵性サツカロミセス酵母の育種 (2008)
  • Author(s): 渡辺誠也, 小瀧努, 牧野圭祐, 松鹿昭則, 澤山茂樹
  • Organizer: 第60回 日本生物工学会大会
  • Place of Presentation: 仙台
  • Year and Date: 2008-09-28
• [Presentation] Reactive oxygen species generation through NADH oxidation by pterin derivatives. (2008)
  • Author(s): M, Nonogawa, T. Arai, N. Endo, G. P. Pack, T. Kodake, K.
  • Organizer: Joint Symposium of 18th International Roundtable on Nucleosides, Nucleotides and Nucleic Acids and
  • Place of Presentation: Kyoto, Japan
  • Year and Date: 2008-09-12
• [Presentation] Repair of DNA-protein crosslink damage: coordinated actions of nucleotide excision repair and homologous recombination. (2008)
  • Author(s): H. Ide, T. Nakano, A.M. Salem, H. Terato, S.P. Pack, and K. Makino
  • Organizer: Joint Symposium of 18th Int. Roundtable on Nucleosides, Nucleotides and Nucleic Acids and 35th Int. Symp. on Nucleic Acids Chemistry
  • Place of Presentation: Kyoto, Japan
  • Year and Date: 2008-09-11
• [Presentation] Efficient preparation of xanthine-containing oligodeoxynucleotide from oxanine-containing oligodeoxynucleotide, catalyzed by N alpha-acetyl-L-histidine. (2008)
  • Author(s): A. Doi, S.P. Pack, T. Kodaki, and K. Makino
  • Organizer: Joint Symposium of 18th Int. Roundtable on Nucleosides, Nucleotides and Nucleic Acids and 35th Int. Symp. on Nucleic Acids Chemistry
  • Place of Presentation: Kyoto, Japan
  • Year and Date: 2008-09-11
• [Presentation] Reactivity of oxanine: efficient fabrication of DNA microanay by using oxanine-containing DNA oligomer as probe molecule. (2008)
  • Author(s): S.P. Pack, N.K. Kamisetty, M. Nonogawa, T. Kodaki, and K. Makino
  • Organizer: Joint Symposium of 18th Int. Roundtable on Nucleosides, Nucleotides and Nucleic Acids and 35th Int. Symp. on Nucleic Acids Chemistry
  • Place of Presentation: Kyoto, Japan
  • Year and Date: 2008-09-11
• [Presentation] Temperature-gradient dependent detection of target DNA oligomers using DNA-immobilized open tubular capillary column. (2008)
  • Author(s): N. K. Kamisetty, S.P. Pack, K.C. Devarayapalli, M. Nonogawa, T. Kodaki, and K. Makino
  • Organizer: Joint Symposium of 18th Int. Roundtable on Nucleosides, Nucleotides and Nucleic Acids and 35th Int. Symp. on Nucleic Acids Chemistry
  • Place of Presentation: Kyoto, Japan
  • Year and Date: 2008-09-11
• [Presentation] Reactive oxygen species generation through NADH oxidation by pterin derivatives (2008)
  • Author(s): M. Nonogawa, T. Arai, N. Endo, S.P. Pack, T. Kodaki, and K. Makino
  • Organizer: Joint Symposium of 18th Int. Roundtable on Nucleosides, Nucleotides and Nucleic Acids and 35th Int. Symp. on Nucleic Acids Chemistry
  • Place of Presentation: Kyoto, Japan
  • Year and Date: 2008-09-11
• [Presentation] Repair of DNA-protein crosslink damage : coordinatedactions of nucleotide excision repair and homologous (2008)
  • Author(s): H. Ide, T. Nakano, A. M. Salem, H. Terato, S. P. Pack, K. Makino.
  • Organizer: Joint Symposium of 18th International Roundtable on Nucleosides, Nucleotides and Nucleic Acids and
  • Place of Presentation: Kyoto, Japan
  • Year and Date: 2008-09-11
• [Presentation] Efficient preparation of xanthine-containing oligodeo xynucleotide from oxanine-containing (2008)
  • Author(s): A. Doi, S. P. Pack, T. Kodaki, K. Makino.
  • Organizer: Joint Symposium of 18ty International Roundtable on Nucleosides, Nucleotides and Nucleic Acids and
  • Place of Presentation: Kyoto, Japan
  • Year and Date: 2008-09-11
• [Presentation] Reactivity of oxanine : efficient fabrication of DNA microarray by using oxanine-containing DNA oligomer as (2008)
  • Author(s): S. P. Pack, N. K. Kamisetty, M. Nonogawa, T. Kodaki, K.
  • Organizer: Joint Symposium of 18th International Roundtable on Nucleosides, Nucleotides and Nucleic Acids and
  • Place of Presentation: Kyoto, Japan
  • Year and Date: 2008-09-11
• [Presentation] Temperature-gradient dependent detection of target DNA oligomers using DNA-immobilized open tubular capillary (2008)
  • Author(s): N. K. Kamisetty, S. P. Pack, K. C. Devarayapalli, M. Nonogawa, T.
  • Organizer: Joint Symposium of 18th International Roundtableon Nucleosides, Nucleotides and Nucleic Acids and
  • Place of Presentation: Kyoto, Japan
  • Year and Date: 2008-09-11
• [Presentation] Bioethanol production from xylose by recombinant Saccharomyces cerevisiae expressing xylose reductase (2008)
  • Author(s): A. Matsushika, S. Watanabe, T. Kodaki, K. Makino, S. Sawavama
  • Organizer: 30th Symposium on Biotechnology for Fuels and Chemicals
  • Place of Presentation: Chicago, USA
  • Year and Date: 2008-05-04
• [Presentation] Oxanine-induced DNA-protein crosslink as an useful biomaterial for elucidation of biological interaction mechanism (2008)
  • Author(s): S.P. Pack, 野々川満、 N. K. Kamisetty, 小瀧努、牧野圭祐
  • Organizer: 日本化学会第88春季年会
  • Place of Presentation: 東京
• [Presentation] 酸塩基触媒アセチルヒスチジンによるオキザニンからキサンチンへの構造変換 (2008)
  • Author(s): 土井昭宏、S. P. Pack, 野々川満、小瀧努、牧野圭祐
  • Organizer: 日本化学会第88春季年会
  • Place of Presentation: 東京
• [Presentation] Development of oligo-DNA coupled micro columns for isolation and detection of DNA oligomers (2008)
  • Author(s): N. K. Kamisetty, S. P. Pack,野々川満、K. C Devarayapalli,和田啓男、小瀧努、牧野圭祐
  • Organizer: 日本化学会第88春季年会
  • Place of Presentation: 東京
• [Presentation] Oxanine-induced DNA-protein crosslink as an useful biomaterial fbr elucidation of biological interaction mechanism (2008)
  • Author(s): S.P. Pack, 野々川満、N. K. Kamisetty, 小龍努、牧野圭祐
  • Organizer: 日本化学会第88春季年会
  • Place of Presentation: 東京
• [Presentation] オキザノシン:シトシン塩基対を含むDNA二重鎖の溶液構造 (2007)
  • Author(s): S.P. Pack,金折賢二、田嶋邦彦、牧野圭祐
  • Organizer: 日本化学会第87春季年会
  • Place of Presentation: 大阪
  • Year and Date: 2007-03-27
• [Presentation] Development of DNA microarray fabrication by efficient surface modification and functional probe design (2007)
  • Author(s): N. K. Kamisetty, S. P. Pack,野々川満、K. C. Devarayapalli,吉田安子、山田和成、渡邊誠也、小瀧努、牧野圭祐
  • Organizer: 日本化学会第87春季年会
  • Place of Presentation: 大阪
  • Year and Date: 2007-03-27
• [Presentation] 新規損傷塩基オキサニンの酵素反応 (2007)
  • Author(s): 土井昭宏、S. P. Pack,野々川満、小瀧努、牧野圭祐
  • Organizer: 日本化学会第87春季年会
  • Place of Presentation: 大阪
  • Year and Date: 2007-03-26
• [Presentation] Repair mechanism of DNA-protein cross-link damage in Escherichia coli (2007)
  • Author(s): T. Nakano, S. Morishita, H. Terato, S. P. Pack, K. Makino, and H. Ide
  • Organizer: 5th International Symposium on Nucleic Acids Chemistry
  • Place of Presentation: Tokyo Japan
• [Presentation] Functional reactivity of oxanine: its biological meanings and biotechnological applications (2007)
  • Author(s): S.P. Pack, A. Doi, N. K. Kamisetty, M. Nonogawa, T. Kodaki, and K. Makino
  • Organizer: 5th International Symposium on Nucleic Acids Chemistry
  • Place of Presentation: Tokyo Japan,
• [Presentation] Development of stable DNA microarray platforms suitable for quantitative analysis (2007)
  • Author(s): N. K. Kamisetty, S. P. Pack, M. Nonogawa, K. C. Devarayapalli, Y. Yoshida, K. Yamada, T. Kodaki, and K. Makino
  • Organizer: 5th International Symposium on Nucleic Acids Chemistry
  • Place of Presentation: Tokyo Japan
• [Presentation] Comparison of enzymatic recognition of DNA-duplexes containing NO-induced lesions by DNA-relevant enzymes (2007)
  • Author(s): A. Doi, S. P. Pack, M. Nonogawa, T. Kodaki and K. Makino
  • Organizer: 5th International Symposium on Nucleic Acids Chemistry
  • Place of Presentation: Tokyo Japan
• [Presentation] Repair mechanism of DNA-protein cross link damage in Escherichia coli (2007)
  • Author(s): T. Nakano, S. Morishita, H. Terato, S. P. Pack, K. Makino, and H. Ide
  • Organizer: 5th International Symposium on Nucleic Acids Chemistry
  • Place of Presentation: Tokyo Japan
• [Presentation] Functional reactivity of oxanine: its biological meanings and biotechnological applications (2007)
  • Author(s): S. P. Pack, A. Doi, N. K. Kamisetty, M. Nonogawa, T. Kodaki, and K. Makino
  • Organizer: 5th International Symposium on Nucleic Acids Chemistry
  • Place of Presentation: Tokyo Japan
• [Presentation] Comparison of enzymatic recognition of DNA-duplexes containing NO-induced lesions by DNA -relevant enzymes (2007)
  • Author(s): A. Doi, S. P. Pack, M. Nonogawa, T. Kodaki and K. Makino
  • Organizer: 5th International Symposium on Nucleic Acids Chemistry
  • Place of Presentation: Tokyo Japan
• [Presentation] Structural property and enzymatic response of oxanine in DNA strands (2006)
  • Author(s): S. P. Pack,土井昭宏、野々川満、N. K. Kamisetty, K. C. Devarayapalli,小瀧努、牧野圭祐
  • Organizer: 第33回核酸化学シンポジウム
  • Place of Presentation: 大阪
  • Year and Date: 2006-11-22
• [Presentation] Chemical synthesis, biochemical and biophysical properties, and biological implication of oxanine-containing oligodeoxynucleotides (2006)
  • Author(s): P. Pack, M. Nonogawa, N. K. Kamisetty, K. C. Devarayapalli, T. Kodaki, and K. Makino
  • Organizer: XVII International Roundtable on Nucleosides, Nucleotides and Nucleic Acids
  • Year and Date: 2006-09-05
• [Presentation] Development of novel pterin derivatives and study of their chemical natures (2006)
  • Author(s): M. Nonogawa, T. Arai, N. Endo, S. P. Pack T. Kodaki, and K. Makino
  • Organizer: Seventh Tetrahedron Symposium
  • Place of Presentation: Kyoto
  • Year and Date: 2006-05-26
• [Presentation] Application of oxanine base for a novel chemical linker in DNA microarray fabrication (2006)
  • Author(s): S. P. Pack, N. K. Kamisetty, M. Nonogawa, T. Kodaki, and K. Makino
  • Organizer: Seventh Tetrahedron Symposium
  • Place of Presentation: Kyoto
  • Year and Date: 2006-05-26
• [Presentation] Fabrication of DNA arrayed capillary system for sensitive and selective analysis of DNA (2006)
  • Author(s): D. K. Charyulu, S. P. Pack, K. N. Kumar; 野々川満、小瀧努、牧野圭祐
  • Organizer: 日本化学会第86春季年会
  • Place of Presentation: 干葉
  • Year and Date: 2006-03-29
• [Book] 微生物の糖代謝経路に見られる新規な進化学的関係(生化学) (2008)
  • Author(s): 渡邊誠也,牧野圭祐
  • Total Pages: 5
  • Publisher: 日本生化学会
• [Book] 「第一章 総論 DNAチップの現状と将来展望」新しいDNAチップの科学と応用 (2007)
  • Author(s): 牧野圭祐
  • Total Pages: 14
  • Publisher: 講談社サイエンティフィク
• [Book] 「第一章 総論 DNAチップの現状と将来展望」、新しいDNAチップの科学と応用 (2007)
  • Author(s): 牧野圭祐
  • Total Pages: 14
  • Publisher: 講談社サイエンティフィク
• [Book] 一酸化窒素によるDNA傷害、別冊・医学のあゆみ、酸化ストレス Ver.2 フリーラジカル医学生物学の最前線(吉川敏一編) (2006)
  • Author(s): 牧野圭祐、Seung Pil Pack
  • Total Pages: 3
  • Publisher: 医歯薬出版
• [Book] 別冊・医学のあゆみ、酸化ストレス (2006)
  • Author(s): 牧野圭祐, Seung Pil Pack
  • Total Pages: 3
  • Publisher: 医歯薬出版
• [Book] 化学フロンティア(17)、新エネルギー最前線、環境調和型エネルギーシステムの構築を目指して (2006)
  • Author(s): 渡邉誠也, 小瀧努, 牧野圭祐
  • Total Pages: 8
  • Publisher: 化学同人