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Study on Mechanism of a Direct Photosynthetic/Metabolic Bio-Fuel Cell (DPBFC) using Gene manipulated Bacteria and Prototyping of Flexible DPBFC

Research Project

Project/Area Number 18360122
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Intelligent mechanics/Mechanical systems
Research InstitutionTokyo University of Agriculture and Technology

Principal Investigator

MORISHIMA Keisuke  Tokyo University of Agriculture and Technology, Institute of Symbiotic Science and Technology, Division of Bio-Applirations and Systems Engineering, Associate Professor (60359114)

Co-Investigator(Kenkyū-buntansha) FURUKAWA Yuji  Tokyo University of Agriculture and Technology, Master of Technology Management, Professor (10087190)
YOSHIDA Makoto  Tokyo Metropolitan University, Graduate School of Science and Engineering, Department of Mechanical Engineering, Assistant Professor (60336518)
Project Period (FY) 2006 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥11,930,000 (Direct Cost: ¥11,600,000、Indirect Cost: ¥330,000)
Fiscal Year 2007: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2006: ¥10,500,000 (Direct Cost: ¥10,500,000)
KeywordsFuel cell / DNA / Bacteria / Micromachine / Bioreactor
Research Abstract

In our laboratory, we have developed a mediator-less direct photosynthetic/metabolic bio-fuel cell (DPBFC) in which microparticles of polyaniline were adopted as the electrode material to extract electrons from bacteria In this paper, we selected Rhodopseudomonas palustris as a new fuel source which is activated by organic compounds and emits hydrogen. To improve the electron generation efficiency of R.palustris, gene manipulation was applied so as to suppress hydrogen emission and more electrons will be stored inside of R.palustris. As a result, the power density obtained by the mutant was 18.3μW/cm^2, which is higher than the wild type. In addition, to extend the cell life, it seems necessary to supply a medium to bacteria continuously, therefore, we have developed a medium flow system using water-absorbing polymer. The average flow rate was 32nl/min and the cell life for the mutant became 12 hours, that is six times longer than the previous DPBFC. In addition, we have prototyped a thin type flexible DPBFC by using a heat-sealing film and thermocompression process. As a result, the developed thin type flexible DPBFC(50×50×1mm) could generate the maximum power 64μW(4.65μW/cm2) when purple photosynthetic bacteria (Rhodopesudomonas palustris) was used as a fuel source.

Report

(3 results)
  • 2007 Annual Research Report   Final Research Report Summary
  • 2006 Annual Research Report
  • Research Products

    (39 results)

All 2008 2007 2006 Other

All Journal Article (15 results) (of which Peer Reviewed: 7 results) Presentation (21 results) Remarks (2 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] Improved Power Capability with Pyrolyzed Carbon Electrodes in Micro Direct Photosynthetic/Metabolic Bio-fuel Cell2008

    • Author(s)
      Takeyuki Moriuchi, Keisuke Morishim and Yuji Furukawa
    • Journal Title

      International Journal Of Precision Engineering And Manufacturing, Korean Society For Precision Engineering Vol.9, No.2

      Pages: 23-27

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Evaluation of Bacteria Compatible Microporous Surfaces for Biofuel Cells2008

    • Author(s)
      Takeyuki Moriuchi, Keisuke Morishima and Yuji Furukawa
    • Journal Title

      CIRP Annals-Manufacturing Technology Vol.57, Issue1

      Pages: 571-574

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Improved Power Capability with Pyrolyzed Carbon Electrodes in Micro Direct Photosynthetic/Metabolic Bio-fuel Cell2008

    • Author(s)
      Takeyuki, Moriuchi, Keisuke, Morishima, Yuii, Furukawa
    • Journal Title

      International Journal Of Precision Engineering And Manufacturing, Korean Society For Precision Engineering Vol. 9, No.2

      Pages: 23-27

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Evaluation of Bacteria Compatible Microporous Surfaces for Biofuel Cells2008

    • Author(s)
      Takeyuki, Moriuchi, Keisuke, Morishima, Yuji, Furukawa
    • Journal Title

      CIRP Annals - Manufacturing Technology Volume 57, Issue 1

      Pages: 571-574

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Improved Power Capability with Pyrolyzed Carbon Electrodes in Micro Direct Photosynthetic/Metabolic Bio-fuel Cell2008

    • Author(s)
      Takeyuki Moriuchi, Keisuke Morishim and Yuji Furukaw
    • Journal Title

      International Journal Of Precision Engineering And Manufacturing, Korean Society For Precision Engineering Vol 9,No.2

      Pages: 23-27

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Evaluation of Bacteria Compatible Microporous Surfaces for Biofuel Cells2008

    • Author(s)
      Takeyuki Moriuchi, Keisuke Morishima and Yuji Furukawa
    • Journal Title

      The International Academy for Production Engineering Accepted

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Improving the performance of a direct photosynthetic/metabolic bio-fuel cell(DPBFC)using gene manipulated bacteria2007

    • Author(s)
      Keisuke Morishima, Makoto Yoshida, Akihiro Furuya, Takeyuki Moriuchi, Masahiro Ota and Yuji Furukawa
    • Journal Title

      Journal of Micromechanics And Microengineering, Institute of Physics Publishing Vol.17, No.9

      Pages: 274-279

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Improving performance of a direct photosynthetic/metabolic bio-fuel cell (DPBFC) using gene manipulated bacteria2007

    • Author(s)
      Keisuke, Morishima, Makoto, Yoshida, Akihiro, Furuya, Takeyuki, Moriuchi, Masahiro, Ota, Yuji, Furukawa
    • Journal Title

      Journal Of Micromechanics And Microengineering, Institute Of Physics Publishing Volume 17, No.9

      Pages: 274-279

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Improving the performance of a direct photosynthetic/metabolic bio-fuel cell (DPBFC) using gene manipulated bacteria2007

    • Author(s)
      Keisuke Morishima, Makoto Yoshida, Akihiro Furuya, Takeyuki Moriuchi, Masahiro Ota and Yuji Furukawa,
    • Journal Title

      Journal Of Micromechanics And Microengineering, Institute Of Physics Publishing 17

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Design prisnciple and prototyping of a direct photosynthetic/metabolic biofuel cell(DPMFC)2006

    • Author(s)
      Yuji Furukawa, Takeyuki Moriuchi and Keisuke Morishima
    • Journal Title

      Journal Of Micromechanics And Microengineering, Institute Of Physics Publishing, Vol.16, No.9

      Pages: 220-225

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Design principle and prototyping of a direct photosynthetic/metabolic biofuel cell (DPMFC)2006

    • Author(s)
      Yuji, Furukawa, Takeyuki, Moriuchi, Keisuke, Morishima
    • Journal Title

      Journal Of Micromechanics And Microengineering, Institute Of Physics Publishing Vol. 16, No. 9

      Pages: 220-225

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Design princicple and protoyping of a direct photosynthetic/metabolic biofuel cell(DPMFC)2006

    • Author(s)
      Yuji Furukawa, Takeyuki Moriuchi, Keisuke Morishima
    • Journal Title

      Journal of Micromechanics and Microengineering Volume16 Number9

      Pages: 220-225

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Improving Performance of Direct Photosynthetic/Metabolic Micro Bio Fuel Cell(DPBFC) by Gene Manipulation of Bacteria2006

    • Author(s)
      Akihiro Furuya, Takeyuki Moriuchi, Makoto Yoshida, Masahiro Ota, Keisuke Morishima, Yuji Furukawa
    • Journal Title

      Proceedings of the 11^th International Conference on Precision Engineering (ICPE), Tokyo, Japan, August, (2006)

      Pages: 197-207

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Genetic Conversion of Bacteria and its Application to Micro Direct Photosynthetic/Metabolic Bio-Fuel Cell(DPMFC)2006

    • Author(s)
      Akihiro Furuya, Takeyuki Moriuchi, Makoto Yoshida, Masahiro Ota, Keisuke Morishima, Yuji Furukawa
    • Journal Title

      Proceedings of the 10^th International Conference on Miniaturized Systems for Chemistry and Life Sciences (μTAS), Tokyo, Japan, November,(2006) Volume 1

      Pages: 482-484

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Improving Performance of Direct Photosynthetic/Metabolic Bio-Fuel Cell(DPBFC) Using Gene Manipulated Bacteria2006

    • Author(s)
      Keisuke Morishima, Akihiro Furuya, Takeyuki Moriuchi, Makoto Yoshida, Masahiro Ota, Yuji Furukawa
    • Journal Title

      Proceedings of the 6^th International Workshop on Micro and Nanotechnology for Power Generation and Energy Conversion Application (PowerMEMS 2006), UC Berkeley, USA, November,(2006)

      Pages: 101-104

    • Related Report
      2006 Annual Research Report
  • [Presentation] 光合成型バイオフューエルセルの出力性能に及ぼす電極構造の影響評価2008

    • Author(s)
      森内 健行, 森島 圭祐, 古川 勇二
    • Organizer
      2008年度精密工学会春季大会
    • Place of Presentation
      神奈川
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
  • [Presentation] 光合成型バイオフューエルセルの性能向上-吸水性ポリマーを用いた培地還流システムの開発2008

    • Author(s)
      古谷 昭博, 森内 健行, 森島 圭祐, 古川 勇二
    • Organizer
      2008年度精密工学会春季大会
    • Place of Presentation
      神奈川
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
  • [Presentation] 光合成細菌を用いたバイオフューエルセルのフレキシブル構造化2008

    • Author(s)
      森内 健行, 角田 史郎, 森島 圭祐, 古川 勇二
    • Organizer
      2008年度精密工学会春季大会
    • Place of Presentation
      神奈川
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
  • [Presentation] Improvement of power capability applying pyrolyzed carbon photoresist electrode for Micro Direct Photosynthetic/metabolic Bio-Fuel Cell(DPBFC)2007

    • Author(s)
      Takeyuki Moriuchi, Keisuke Morishima and Yuji Furukawa
    • Organizer
      Asian Symposium for Precision Engineering and Nanotechnology2007
    • Place of Presentation
      GIST, Korea
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Porous Carbon Electrode To Improve Power Of Photosynthetic Bacteria Fuel Cell2007

    • Author(s)
      Takeyuki Moriuchi, Keisuke Morishima and Yuji Furukawa
    • Organizer
      PowerMEMS 2007
    • Place of Presentation
      Congress and Convention Center, Germany
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
  • [Presentation] Development of Flexible Direct Photosynthetic/metabolic Bio-fuel Cell(DPBFC)for Mobile Use2007

    • Author(s)
      Takeyuki Moriuchi, Shiro Sumida, Akihiro Furuya, Keisuke Morishima and Yuji Furukawa
    • Organizer
      Asian Symposium for Precision Engineering and Nanotechnology 2007,
    • Place of Presentation
      GIST, Korea,
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 直接光合成/代謝型バイオ燃料電池に関する研究-フレキシブル・モバイル形状の開発-2007

    • Author(s)
      角田 史郎, 古谷 昭博, 森内 健之, 吉田 真, 森島 圭祐, 古川 勇二
    • Organizer
      第15回化学とマイクロ・ナノシステム研究会,
    • Place of Presentation
      仙台
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Improvement of power capability applying pyrolyzed carbon photoresist electrode for Micro Direct Photosynthetic/metabolic Bio-Fuel Cell(DPBFC)2007

    • Author(s)
      Takeyuki, Moriuchi, Keisuke, Morishima, Yuji, Furukawa
    • Organizer
      Asian Symposium for Precision Engineering and Nanotechnology 2007 GIST
    • Place of Presentation
      Korea
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Porous Carbon Electrode To Improve Power Of Photosynthetic Bacteria Fuel Cell2007

    • Author(s)
      Takeyuki, Moriuchi, Keisuke, Morishima, Yuji, Furukawa
    • Organizer
      owerMEMS 2007, Congress and Convention Center
    • Place of Presentation
      Germany
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Development of Flexible Direct Photosynthetic/metabolic Bio-fuel Cell (DPBFC) for Mobile Use2007

    • Author(s)
      Takeyuki, Moriuchi, Shiro, Sumida, Akihiro, Furuya, Keisuke, Morishima, Yuji, Furukawa
    • Organizer
      Asian Symposium for Precision Engineering and Nanotechnology 2007, GIST
    • Place of Presentation
      Korea
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Improvement of power capability applying pyrolyzed carbon photoresistelectrode for Micro Direct Photosynthetic/metabolic Bio-Fuel Cell (DPBFC)2007

    • Author(s)
      Takeyuki Moriuchi, Keisuke Morishima and Yuji Furukawa
    • Organizer
      Asian Symposium for Precision Engineering and Nanotechnology 2007
    • Place of Presentation
      GIST, Korea
    • Related Report
      2007 Annual Research Report
  • [Presentation] Development of Flexible Direct Photosynthetic/metabolic Bio-fuel Cell (DPBFC) for Mobile Use2007

    • Author(s)
      Takeyuki Moriuchi, Shiro Sumida, Akihiro Furuya, Keisuke Morishima and Yuji Furukawa
    • Organizer
      Asian Symposium for Precision Engineering and Nanotechnology 2007,
    • Place of Presentation
      GIST, Korea,
    • Related Report
      2007 Annual Research Report
  • [Presentation] 直接光合成/代謝型バイオ燃料電池に関する研究 -フレキシブル・モバイル形状の開発-2007

    • Author(s)
      角田 史郎, 古谷 昭博, 森内 健之, 吉田 真, 森島 圭祐, 古川 勇二
    • Organizer
      第15回 化学とマイクロ・ナノシステム研究会,
    • Place of Presentation
      仙台
    • Related Report
      2007 Annual Research Report
  • [Presentation] Improving Performance of Direct Photosynthetic/Metabolic Micro Bio Fuel Cell(DPBFC)by Gene Manipulation of Bacteria2006

    • Author(s)
      Akihiro Furuya, Takeyuki Moriuchi, Makoto Yoshida, Masahiro Ota, Keisuke Morishima and Yuji Furukawa
    • Organizer
      11th International Conference on Precision Engineering(11th ICPE)
    • Place of Presentation
      Tokyo
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Genetic Conversion of Bacteria and its Application to Micro Direct Photosynthetic/Metabolic Bio-Fuel Cell(DPMFC)2006

    • Author(s)
      Akihiro Furuya, Takeyuki Moriuchi Makoto Yoshida, Masahiro Ota Keisuke Morishima and Yuji Furukawa
    • Organizer
      The 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences(μTAS)
    • Place of Presentation
      Tokyo
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Improving Performance of Direct Photosynthetic/Metabolic Bio-Fuel Cell(DPBFC)Using Gene Manipulated Bacteria2006

    • Author(s)
      Keisuke Morishima, Akihiro Furuya, Takeyuki Moriuchi, Makoto Yoshida, Masahiro Ota and Yuji Furukawa
    • Organizer
      The sixth International Workshop on Micro and Nanote chnology for Power Generation and Energy Conversion Applications(PowerMEMS 2006),
    • Place of Presentation
      UC Berkeley, USA
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 直接光合成/代謝方式によるマイクロバイオフューエルセルの発電原理と試作2006

    • Author(s)
      森内 健行, Yujie Han, 森島 圭祐, 古川 勇二
    • Organizer
      2006年度精密工学会春季大会
    • Place of Presentation
      東京
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 直接光合成・代謝型マイクロバイオフューエルセルの開発-遺伝子操作細菌と小型化による性能向上-2006

    • Author(s)
      古谷 昭博, 森内 健行, 吉田 真, 森島 圭祐, 太田 正廣, 古川 勇二
    • Organizer
      ロボティクス・メカトロニクス公演会2006
    • Place of Presentation
      東京
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Improving Performance of Direct Photosynthetic/Metabolic Micro Bio Fuel Cell (DPBFC) by Gene Manipulation of Bacteria2006

    • Author(s)
      Akihiro, Furuya, Takeyuki, Moriuchi, Makoto, Yoshida, Masahiro, Ota, Keisuke, Morishima, Yuji, Furukawa
    • Organizer
      11th International Conference on Precision Engineering (11th ICPE)
    • Place of Presentation
      Tokyo, Japan
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Genetic Conversion of Bacteria and its Application to Micro Direct Photosynthetic/Metabolic Bio-Fuel Cell (DPMFC)2006

    • Author(s)
      Akihiro, Furuya, Takeyuki, Moriuchi, Makoto, Yoshida, Masahiro, Ota, Keisuke, Morishima, Yuji, Furukawa
    • Organizer
      The 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences(μTAS)
    • Place of Presentation
      Japan
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Improving Performance of Direct Photosynthetic/Metabolic Bio-Fuel Cell (DPBFC) Using Gene Manipulated Bacteria2006

    • Author(s)
      Keisuke, Morishima, Akihiro, Furuya, Takeyuki, Moriuchi, Makoto, Yoshida, Masahiro, Ota, Yuji, Furukawa
    • Organizer
      The sixth International Workshop on Micro and Nanotechnology for Power Generation and Energy Conversion Applications(PowerMEMS 2006)
    • Place of Presentation
      UC Berkeley, USA
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Remarks] 「研究成果報告書概要(和文)」より

    • URL

      http://www.tuat.ac.jp/~biomems/

    • Related Report
      2007 Final Research Report Summary
  • [Remarks]

    • URL

      http://www.tuat.ac.jp/~biomems/

    • Related Report
      2007 Annual Research Report
  • [Patent(Industrial Property Rights)] バイオ燃料電池用電極およびバイオ燃料電池2006

    • Inventor(s)
      古川 勇二, 森島 圭祐, 古谷 昭博, 森内 健行, 吉田 真, 大田 正廣
    • Filing Date
      2006-06-01
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary

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Published: 2006-04-01   Modified: 2016-04-21  

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