Development of a micro-bioreactor system for chiral synthesis incorporated with a function of optical resolution

• Project/Area Number: 18360397
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Biofunction/Bioprocess
• Research Institution: Kyushu University
• Principal Investigator: KAWAKAMI Koei Kyushu University, 大学院・工学研究院, 教授 (70091345)
• Co-Investigator(Kenkyū-buntansha): SAKAI Shinji 九州大学, 大学院・工学研究院, 助教 (20359938) ; 岩井 芳夫 九州大学, 大学院・工学研究院, 准教授 (80176528)
• Co-Investigator(Renkei-kenkyūsha): IWAI Yoshio 九州大学, 大学院・工学研究院, 准教授 (80176528)
• Project Period (FY): 2006 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥17,610,000 (Direct Cost: ¥15,600,000、Indirect Cost: ¥2,010,000); Fiscal Year 2008: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2007: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2006: ¥8,900,000 (Direct Cost: ¥8,900,000)
• Keywords: バイオリアクター / 生物・生体工学 / 生体触媒 / マイクロバイオリアクター / シリカモノリス / 光学分割 / リパーゼ / 反応・分離 / ゾルーゲル法 / ゾル-ゲル / 不斉合成

Research Abstract

メチル基含有マクロポーラスシリカモノリスの調製とリパーゼを含むブチル基含有シリカによる内部表面塗布からなる2段階ゾルーゲル法を利用して、リパーゼ固定化シリカモノリスを装填した高効率マイクロバイオリアクターを開発した。有機溶媒中のグリシドールと酪酸ビニルのエステル交換に対する流通操作において、本固定化リパーゼは単なるシリカモノリス付着リパーゼより約10倍高い活性を示した。このモノリスバイオリアクターに市販のキラルカラムを直列に結合し、基質溶液のパルス供給を行ったところ、ラセミ体エステルの光学分割が達成できることがわかった。また、一本のモノリスカラムにリパーゼとキラル分離剤を共固定化することにより、エステル合成と光学分割が同時進行する反応・分離一体型システム構築の可能性について検討した。

Research Products

• [Journal Article] Application of a lipase-immobilized silica monolith bioreactor to the production of fatty acid methyl esters (2009)
  • Author(s): Koei Kawakami, Ryo Takahashi, Mozaffar Shakeri, Shinji Sakai
  • Journal Title: Journal of Molecular Catalysis B : Enzymatic Vol.57 Pages: 194-197
  • Peer Reviewed
• [Journal Article] Application of a lipase-immobilized silica monolith bioreactor to the production of fatty acid methyl esters (2009)
  • Author(s): Koei Kawakami, et al.
  • Journal Title: Journal of Molecular Catalysis B ; Enzymatic 57 Pages: 194-197
  • Peer Reviewed
• [Journal Article] Activation of lipase by sol-gel coating with hydrophobic alkyl-substituted silicates in supercritical carbon dioxide (2009)
  • Author(s): Koei Kawakami, et al.
  • Journal Title: Journal of Chemical Technology and Biotechnology (in press)
  • Peer Reviewed
• [Journal Article] Develop-ment of a silica monolith micro-bioreactor entrapping highly activated lipase and an experiment towardintegration with chromatographic separation of chiral esters (2007)
  • Author(s): Koei Kawakami, Daisuke Abe, Taiki Urakawa, Ayako Kawashima, Yasuhiro Oda, Ryo Takahashi, Shinji Sakai
  • Journal Title: Journal of Separation Science Vol.30 Pages: 3077-3084
  • Peer Reviewed
• [Journal Article] Development of a silica monolith microbioreactor entrapping highly activated lipase and an experiment toward integration with chromatographic separation of chiral esters (2007)
  • Author(s): Kawakami, et. al.
  • Journal Title: Journal of Separation Science 30 Pages: 3077-3084
  • Peer Reviewed
• [Journal Article] 生体物質の機能発現に有効なゾル-ゲルシリカの新規応用 (2006)
  • Author(s): 川上幸衛、境慎司
  • Journal Title: 化学工業 57巻12号 Pages: 43-47
• [Journal Article] Development and characterization of a lipase-immobilized silica monolith micro-capillary reactor prepared by using a two-step in situ sol-gel method (2006)
  • Author(s): Koei Kawakami
  • Journal Title: Book of Abstracts, 6^<th> European Symposium on Biochemical Engineering Science Pages: 19-19
• [Journal Article] Fabrication of a silica monolith micro-bioreactor entrapping highly activated lipase by a two-step sol-gel method (2006)
  • Author(s): Koei Kawakami et al.
  • Journal Title: Proceedings of 17^<th> International Congress of Chemical and Process Engineering (in CD-ROM edition) Pages: 9-9
• [Journal Article] Fabrication of a silica monolith micro-bioreactor entrapping highly activated lipase by a two-step sol-gel method (2006)
  • Author(s): Koei Kawakami et al.
  • Journal Title: Book of Abstracts, 19^<th> International Symposium on Chemical Reaction Engineering Pages: 407-408
• [Journal Article] 生体物質の機能発現に有効なゾル-ゲルシリカの新規応用 (2006)
  • Author(s): 川上幸衞, 境慎司
  • Journal Title: 化学工業 57巻・12号 Pages: 931-935
• [Journal Article] Activation of lipase by sol-gel coating with hydrophobic alkyl-substituted silicates in supercritical carbon dioxide
  • Author(s): Koei Kawakami, Taiki Urakawa, Yasuhiro Oda, Yoshio Iwai
  • Journal Title: Journal of Chemical Technology and Biotechnology (in press)
  • Peer Reviewed
• [Presentation] リパーゼ固定化シリカモノリスバイオリアクターの特性評価およびバイオディーゼル生産への応用 (2009)
  • Author(s): 高橋涼ら
  • Organizer: 化学工学会第74年会
  • Place of Presentation: 横浜国立大学、 横浜
  • Year and Date: 2009-03-18
• [Presentation] リパーゼ固定化シリカモノリスバイオリアクターの特性評価およびバイオディーゼル生産への応用 (2009)
  • Author(s): 高橋涼、武井孝行、境慎司、井嶋博之、川上幸衛
  • Organizer: 化学工学会第74年会
• [Presentation] Application of a lipase-immobilized silica monolith bioreactor to production of fatty acid alkyl esters (2008)
  • Author(s): Ryo Takahashi, et al.
  • Organizer: The 21^<st> International Symposium on Chemical Engineering
  • Place of Presentation: 佐賀大学、 佐賀
  • Year and Date: 2008-12-06
• [Presentation] リパーゼ固定化シリカモノリスバイオリアクターによるバイオディーゼルの生産 (2008)
  • Author(s): 高橋涼ら
  • Organizer: 化学工学会3支部合同姫路大会
  • Place of Presentation: ホテル日航姫路、 姫路
  • Year and Date: 2008-11-18
• [Presentation] シリカモノリス固定化リパーゼによるトリグリセリドのメタノリシス (2008)
  • Author(s): 高橋涼、川島綾子、境慎司、井嶋博之、川上幸衛
  • Organizer: 第10回化学工学会学生発表会
• [Presentation] Application of a lipase-immobilized silica monolith bioreactor to production of fatty acid alkyl esters (2008)
  • Author(s): Koei Kawakami, Ryo Takahashi, Mozaffar Shakeri, Shinji Sakai
  • Organizer: 4th International Congress on Biocatalysis
• [Presentation] Alcoholysis of triglycerides in a silica monolith bioreactor entrapping highly activated lipase (2008)
  • Author(s): Koei Kawakami, Ryo Takahashi, Mozaffar Shakeri, Shinji Sakai
  • Organizer: 7th European Symposium on Biochemical Engineering Science
• [Presentation] リパーゼ固定化シリカモノリスバイオリアクターによるバイオディーゼルの生産 (2008)
  • Author(s): 高橋涼、武井孝行、境慎司、井嶋博之、川上幸衛
  • Organizer: 化学工学会3支部合同姫路大会
• [Presentation] Development of a lipase-immobilized silica monolith bioreactor and its application to the production of biodiesel (2008)
  • Author(s): Ryo Takahashi, Takayuki Takei, Shinii Sakai, Hiroyuki Ijima, Koei Kawakami
  • Organizer: The 21^<st> International Symposium on Chemical Engineering
• [Presentation] Application of a lipase-immobilized silica monolith bioreactor to production of fatty acid alkyl esters (2008)
  • Author(s): Koei Kawakami, et al.
  • Organizer: 4th International Congress on Biocatalysis
  • Place of Presentation: ハンブルグ工業人学、 ハンブルグ、 ドイツ
• [Presentation] Alcoholysis of triglycerides in a silica monolith bioreactor entrapping highly activated lipase (2008)
  • Author(s): Koei Kawakami, et al.
  • Organizer: 7th European Symposium on Biochemical Engineering Science
  • Place of Presentation: アルガルベ大学、 フアロ、 ポルトガル
• [Presentation] Development of a lipase-immobilized silica monolith micro-bioreactor accompanied by chromatographic separation of chiral esters (2007)
  • Author(s): Kawashima, et. al.
  • Organizer: Asia Pacific Biochemical Engineering Conference, APBioChEC'07
  • Place of Presentation: Taipei, Taiwan
  • Year and Date: 2007-11-06
• [Presentation] Development and characterization of a lipase-immobilized silica monolith micro-capillary reactor prepared by using a two-step in situ sol-gel method (2007)
  • Author(s): Kawakami, et. al.
  • Organizer: 1^<st> International Congress on Green Process Engineering
  • Place of Presentation: Toulouse, France
  • Year and Date: 2007-04-25
• [Presentation] Development and characterization of a lipase-immobilized silica monolith micro-capillary reactor prepared by using a two-step in situ sol-gel method (2007)
  • Author(s): Koei Kawakami, Taiki Urakawa, Ayako Kawashima
  • Organizer: 1st International Congress on Green Process Engineering
• [Presentation] 超臨界二酸化炭素中におけるシリカ被覆リパーゼの調製と特性 (2007)
  • Author(s): 織田康裕、境慎司、井嶋博之、川上幸衛
  • Organizer: 第44回化学関連支部合同九州大会
• [Presentation] Develop-ment of gel materials congruous with functional biological substances (2007)
  • Author(s): Koei Kawakami, Shinji Sakai
  • Organizer: The 1st International Symposium for Future Molecular Systems
• [Presentation] Development of a lipase-immobilized silica monolith micro-bioreactor accompanied by chromatographic separation of chiral esters (2007)
  • Author(s): Ayako Kawashima, Taiki Urakawa, Shinji Sakai, Hiroyuki Ijima, Koei Kawakami
  • Organizer: Asia-Pacific Biochemical Engineering Conference
• [Presentation] リパーゼ固定化シリカモノリスの調製と反応-分離一体型システムの構築 (2007)
  • Author(s): 川島綾子、境慎司、井嶋博之、川上幸衛
  • Organizer: 日本生物工学会九州支部長崎大会
• [Presentation] Development and chara-cterization of a lipase-immobilized silica monolith micro-capillary reactor prepared by using a two-step in situ sol-gel method (2006)
  • Author(s): Koei Kawakami
  • Organizer: 6th European Symposium on Biochemical Engineering Science
• [Presentation] Fabrication of a silica monolith micro-bioreactor entrapping highly activated lipase by a two-step sol-gel method (2006)
  • Author(s): Koei Kawakami, Daisuke Abe, Taiki Urakawa, Shinji Sakai, Tsutomu Ono, Hiroyuki Ijima
  • Organizer: 17th International Congress of Chemical and Process Engineering
• [Presentation] Fabrication of a silica monolith micro-bioreactor entrapping highly activated lipase by a two-step sol-gel method (2006)
  • Author(s): Koei Kawakami, Daisuke Abe, Taiki Urakawa, Shinji Sakai, Tsutomu Ono, Hiroyuki Ijima
  • Organizer: 19th International Symposium on Chemical Reaction Engineering
• [Presentation] 二段階ゾル-ゲル法によるリパーゼ固定化シリカモノリスマイクロバイオリアクターの調製と特性 (2006)
  • Author(s): 浦川大樹、安部大輔、境慎司、小野勉、井嶋博之、川上幸衛
  • Organizer: 化学工学会第38回秋季大会
• [Presentation] Performance of a lipase-immobilized silica monolith micro-bioreactor prepared by a two-step sol-gel method (2006)
  • Author(s): Ayako Kawashima, Tsutomu Ono, Shinji Sakai, Hiroyuki Ijima, Koei Kawakami
  • Organizer: The 19th International Symposium on Chemical Engineering
• [Book] ゾル-ゲル法および有機-無機ハイブリッド材料-構造制御・高性能化とその応用(ゾル-ゲルシリカのバイオ分野への新規応用) (2007)
  • Author(s): 川上幸衛、境慎司、山口哲
  • Publisher: 技術情報協会
• [Remarks]
  • URL: http://sirius.chem-eng.kyushu-u.ac.jp/
• [Remarks]
  • URL: http://www.cstm.kyushu-u.ac.jp/gcoe/jpn/members/member.php?id=25
• [Remarks]
  • URL: http://sirius.chem-eng.kyushu-u.ac.jp/
• [Remarks]
  • URL: http://www.cstm.kyushu-u.ac.jp/gcoe/jpn/members/member.php?id=25
• [Remarks]
  • URL: http://sirius.chem-eng.kyushu-u.ac.jp/
• [Remarks]
  • URL: http://www.cstm.kyushu-u.ac.jp/gcoe/jpn/members/member.php?id=25
• [Patent(Industrial Property Rights)] 有機質粒子とポリメタロキサン系材料との複合体及びその製造方法 (2007)
  • Inventor(s): 川上幸衛ら他5名
  • Industrial Property Rights Holder: 株式会社豊田中央研究所
  • Acquisition Date: 2007-08-02