Spatio-temporal regulation of the formation and function of the yeast 26S proteasome

• Project/Area Number: 18370002
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Genetics/Genome dynamics
• Research Institution: Tokyo Metropolitan Organization for Medical Research
• Principal Investigator: TOUE Akio Tokyo Metropolitan Organization for Medical Research, 東京都臨床医学総合研究所, 研究員 (90029249)
• Project Period (FY): 2006 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥17,220,000 (Direct Cost: ¥15,000,000、Indirect Cost: ¥2,220,000); Fiscal Year 2008: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2007: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2006: ¥7,600,000 (Direct Cost: ¥7,600,000)
• Keywords: プロテアソーム / 出芽酵母 / 分子集合 / リッド / ベース / 26Sプロテアソーム / パン酵母 / マス解析 / 黒カビ / 高温生育性 / 蛍光タイフーン / サブユニット融合変異株 / 結晶化 / 1粒子解析 / 電子顕微鏡 / 19S調節因子(19SRP) / 温度感受性変異 / 局在

Research Abstract

酵母の26Sプロテアソームの調節因子(RP)はリッドとベースという二つの複合体からなる。これまで,我々は、これらの複合体を構成するサブユニット遺伝子に関する温度感受性変異体を分離・集積してきた。今回、リッド、および、ベースの変異体を生化学的および細胞生物学的な方法で解析し、これらの複合体が互いに独立に形成され核内に移送され、RPが形成されることを明らかにした。26Sプロテアソーム研究の新奇材料として、高温で飼育可能なカビAspergillusu fumigatusの26Sプロテアソームの精製法を確立し,サブユニット構成を明らかにした。

Research Products

• [Journal Article] Lysine 63-linked polyubiquitin chain may serves the targeting signal for the 26S proteasome. (2009)
  • Author(s): Saeki, Y., Kudo, T., Sone, T., Kikuchi, Y., Yokosawa, H., Toh-e, A., Tanaka, K.
  • Journal Title: EMBO J 28 Pages: 359-371
  • Peer Reviewed
• [Journal Article] Lysine 63-linked polyubiquitin chain serves as a targeting signal for the 26S proteasome (2009)
  • Author(s): Saeki, Y., Kudo, T., Sone, T., Yokosawa, H., Toh-e, A., Tanaka, K.
  • Journal Title: EMBO J 28 Pages: 359-371
  • Peer Reviewed
• [Journal Article] 26S proteasome regulatory particle mutants have increased oxidative stress tolerance (2008)
  • Author(s): Kurepa J, Toh-e A, Smalle JA.
  • Journal Title: Plant J. 53(1) Pages: 102-14
  • Peer Reviewed
• [Journal Article] Transcriptional repression by the Pho4 transcription factor controls the timing of SNZ1 expression. (2008)
  • Author(s): Nishizawa, M., Komai, T., Morohashi, N. Shimizu, M. Toh-e, A.
  • Journal Title: Eukaryot Cell 7 Pages: 949-957
  • Peer Reviewed
• [Journal Article] Nutrient-regulated antisense and intragenic RNAs modulate a signal transduction pathway in yeast (2008)
  • Author(s): Nishizawa M, Komai T, Katou Y, Shirahige K, Ito T, Toh-e A.
  • Journal Title: PLoS Biol. 6 Pages: 2817-30
  • Peer Reviewed
• [Journal Article] Transcriptional repression by the Pho4 transcriptional factor controls the timing of SNZ1 expression (2008)
  • Author(s): Nishizawa, M., Komai, T., Moro-Hashi, N., Shimizu, M., Toh-e, A.
  • Journal Title: Eukaryot Cell 7 Pages: 949-957
  • Peer Reviewed
• [Journal Article] Local anesthetics, antipsychotic phenothiazines, and cationic surfactants shut down intracellular reactions through membrane perturbation in yeast (2008)
  • Author(s): Uesono, Y., Araki, T., Toh-e, A
  • Journal Title: Biosci. Biotechnol. Biochem. 72 Pages: 2884-2894
  • Peer Reviewed
• [Journal Article] 26S proteasome regulatory particle mutants have increased oxidative stress tolerance. (2008)
  • Author(s): Kurepa, J., Toh-e, A., Smalle, J., A.
  • Journal Title: Plant J 53 Pages: 102-114
  • Peer Reviewed
• [Journal Article] The assembly pathway of the 19S regulatory particle of the yeast 26S proteasome (2007)
  • Author(s): Isono E, Nishihara K, Saeki Y, Yashiroda H,Kamata N, Ge L, Ueda T, Kikuchi Y, Tanaka K, Nakano A, Toh-e A.
  • Journal Title: Mol Biol Cell 18 Pages: 569-80
  • Peer Reviewed
• [Journal Article] A role for Ltelp (a low temperature essential protein involved in mitosis) in proprotein processing in the yeast secretory pathway (2007)
  • Author(s): Zhao X, Chang AY, Toh-e A, Arvan P.
  • Journal Title: J Biol Chem. 282 Pages: 1670-8
  • Peer Reviewed
• [Journal Article] The assembly pathway of the 19S regulatory particle of the yeast 26S proteasome. (2007)
  • Author(s): Isono, E., Nishihara, K., Saeki, Y., Yashiroda, H., Kamata, N., Ge, L., Ueda, T., Kikuchi, Y., Tanaka, K., Nakano, A., Toh-e, A.
  • Journal Title: Mol.Biol.Cell 18 Pages: 569-580
  • Peer Reviewed
• [Journal Article] A role for Ltelp (a low temperature essential protein involved in mitosis) in proprotein processing in the yeast secretory pathway. (2007)
  • Author(s): Zhao X, Chang AY, Toh-E A, Arvan P
  • Journal Title: J. Biol. Chem. 282 Pages: 1670-1778
• [Journal Article] The Assembly Pathway of the 19S Regulatory Particle of the Yeast 26S Proteasome. (2007)
  • Author(s): Isono E, Nishihara K, Saeki Y, Yashiroda H, Kamata N, Ge L, Ueda T, Kikuchi Y, Tanaka K, Nakano A, Toh-E A
  • Journal Title: Mol. Biol. Cell 18 Pages: 569-580
• [Journal Article] Identifying novel substrates for mouse CDK5 using the yeast Saccharomyces cerevisiae. (2006)
  • Author(s): Horiuchi, Y., Asada, A., Hisanaga, S., Toh-e, A., Nishizawa,M.
  • Journal Title: Genes Cells 11 Pages: 1393-1404
• [Journal Article] 出芽酵母26S プロトアソームの分子集合 (2006)
  • Author(s): 磯野江利香, 東江昭夫
  • Journal Title: 蛋白質・核酸・酵素 51 Pages: 1224-1229
• [Presentation] Lysine 63-linked polyubiquitin chain serves as a targeting signal for the 26S proteasome. (2008)
  • Author(s): Saeki, Y., Yokosawa, H., Toh-e, A., Tanaka, K.
  • Organizer: The 5^<th> International Symposium on the COP9 signalosome, Proteasome, and eIF3 : At the interface between signal and proteolysis
  • Place of Presentation: RIKEN Yokohama, Japan
• [Presentation] Lysine 63-linked polyubiquitin chain serves a targeting signal for the 26S proteasome (2008)
  • Author(s): Sseki, Y., Yokosawa, H., Toh-e, A., Tanaka, K
  • Organizer: The 5^<th> International Symposium on the COP9 signalosome, PRoteasome, and eIF3 : At the interface between signal and proteolysis
  • Place of Presentation: RIKEN Yokohama, Japan
• [Book] Biogenesis of the 26S proteasome in the yeast Saccharomyces cerevisiae. Tanpakushitsu Kakusan Koso. (2006)
  • Author(s): Isono E, Toh-e A.
  • Publisher: 共立出版株式会社