Studies for regulation mechanism of meiotic crossover formation through the protein-chromosome structure

• Project/Area Number: 18370003
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Genetics/Genome dynamics
• Research Institution: Osaka University
• Principal Investigator: SHINOHARA Miki Osaka University, 蛋白質研究所, 准教授 (80335687)
• Project Period (FY): 2006 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥16,910,000 (Direct Cost: ¥14,000,000、Indirect Cost: ¥2,910,000); Fiscal Year 2008: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000); Fiscal Year 2007: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000); Fiscal Year 2006: ¥4,300,000 (Direct Cost: ¥4,300,000)
• Keywords: 減数分裂期 / 相同組換え / シナプトネマ複合体 / 減数分裂 / リン酸化制御

Research Abstract

減数分裂期に発現するSpo16 タンパク質が、シナプトネマ複合体と呼ばれる減数分裂期に形成される染色体高次構造体の新規構成因子であることを明らかにした。配偶子を作るとき、染色体数を半数にするために、キアズマを染色体上で正しく配置する仕組み(干渉)と全ての染色体にキアズマを必ず作る仕組み(保証)がある。シナプトネマ複合体が干渉と保証の両方を制御するがそれぞれに異なるユニットを用いることを明らかにした。

Research Products

• [Journal Article] 減数分裂期組換え制御の分子メカニズム (2009)
  • Author(s): 篠原美紀、篠原彰
  • Journal Title: 蛋白質核酸酵素 54 Pages: 466-471
• [Journal Article] 減数分裂期組換え制御の分子メカニズム (2009)
  • Author(s): 篠原美紀
  • Journal Title: 蛋白質核酸酵素 54 Pages: 466-471
• [Journal Article] Csm4-dependent telomere movement onnuclear envelope promotes meiotic recombination (2008)
  • Author(s): Kosaka, Hiromichi, Miki Shinohara and Akira Shinohara
  • Journal Title: PLoS Genet. 4 : e1000196
• [Journal Article] Rapid telomere movement in meioticprophase is promoted by NDJ1, MPS3 andCSM4 and is modulated by recombination (2008)
  • Author(s): Conrad, Michael, Chih-Ying Lee, GeneChao, Miki Shinohara, Hiromichi Kosaka, Akira Shinohara, J.A.Conchello, and Michel E.Dresser
  • Journal Title: Cell 133 Pages: 1175-87
• [Journal Article] FHA domain of yeast Xrs2, a homologue ofhuman Nbs1, promotes non-homologousend joining through the interaction with aligase IV partner protein, Lif1 (2008)
  • Author(s): Matsuzaki, Kenichiro, Akira Shinohara andMiki Shinohara
  • Journal Title: Genetics 179 Pages: 213-25
• [Journal Article] Rad52 promotes postinvasion steps ofmeiotic double-strand-break repair (2008)
  • Author(s): Lao, Jeccica P, Steve D.Oh, Miki Shinohara, Akira Shinohara, Neil Hunter
  • Journal Title: Mol.Cell 29 Pages: 517-524
• [Journal Article] Crossover assurance and crossoverinterference are distinctly regulated by theZMM/SIC proteins during meiosis (2008)
  • Author(s): Shinohara, Miki, Steve D. Oh, Neil Hunterand Akira Shinohara
  • Journal Title: Nature Genet 40 Pages: 299-309
• [Journal Article] タンパク質リン酸化を介したDNA 二重鎖切断修復制御のメカニズム (2008)
  • Author(s): 篠原美紀
  • Journal Title: 生化学 80 Pages: 1029-1033
  • NAID: 10024455011
• [Journal Article] FHA domain of yeast Xrs2, a homolog of human Nbs1, promotes non-homologous end joining through the interaction with a Ligase-IV partner protein, Lifl (2008)
  • Author(s): Matsuzaki, K
  • Journal Title: Genetics 179 Pages: 213-215
  • Peer Reviewed
• [Journal Article] Rapid telomere movement in meiotic prophase is promoted by NDJ1, MPS3 and CSM4 amd is modurated by recombination (2008)
  • Author(s): Conrad, M
  • Journal Title: Cell 133 Pages: 1175-1187
  • Peer Reviewed
• [Journal Article] Csm4-dependent telomere movement on nuclear envelope promotes meiotic recombination (2008)
  • Author(s): Kosaka, H
  • Journal Title: PLoS Genet 4
  • Peer Reviewed
• [Journal Article] タンパク質リン酸化を介したDNA二重鎖切断制御の分子メカニズム (2008)
  • Author(s): 篠原美紀
  • Journal Title: 生化学 80 Pages: 1029-1033
  • Peer Reviewed
• [Journal Article] Crossover assurance and crossover interference are distinctly regulated by the ZMM proteins during meiosis. (2008)
  • Author(s): Shinohara, M
  • Journal Title: Nature Genetics 40 Pages: 299-309
  • Peer Reviewed
• [Journal Article] Rad52 promotes postinvasion steps of meiotic dounle-strand-break repair (2008)
  • Author(s): Lao J
  • Journal Title: Mol. Cell 29 Pages: 517-524
  • Peer Reviewed
• [Journal Article] FHA domain of yeast Xrs2, a homologue of human Nbs1, promotes non-homologous end joining through the interaction with a Ligase IV partner protein, Lifl (2008)
  • Author(s): Matsuzaki K
  • Journal Title: Genetics (in press)
  • Peer Reviewed
• [Journal Article] Meiotic recombination-related DNAsynthesis and its implications for cross-overand non-cross-over recombinant formation (2007)
  • Author(s): Terasawa, Masahiro, Hideyuki Ogawa, Yasumasa Tsukamoto, Miki Shinohara, Katsuhiko Shirahige, Nancy Kleckner andTomoko Ogawa
  • Journal Title: Proc. Natl. Acad. Sci. USA. 104 : (14) Pages: 5965-5970
• [Journal Article] Distinct functions of the twospecificity-determinants in replicationinitiation of the plasmids ColE2-P9 andColE3-CA38 (2007)
  • Author(s): Aoki, Kazuaki, Miki Shinohara and Tateo Itoh
  • Journal Title: J Bacteriol 19 Pages: 2392-2400
• [Journal Article] Distinct functions of the two specificity-determinants in replication initiation of the plasmids ColE2-P9 and ColE3-CA38 (2007)
  • Author(s): Aoki, Kazuaki
  • Journal Title: J Bacteriol 19 Pages: 2392-2400
  • Peer Reviewed
• [Journal Article] Meiotic recombination-related DNA synthesis and its implications forcross-over and non-cross-over recombinant formation (2007)
  • Author(s): Terasawa, M
  • Journal Title: Proc. Natl. Acad. Sci. USA 104 Pages: 5965-5970
  • Peer Reviewed
• [Journal Article] Distinct functions of the two specificity-determinants in replication initiation of the plasmids ColE2-P9 and ColE3-CA38 (2007)
  • Author(s): Aoki, Kazuaki, Miki Shinohara, Tateo Itoh
  • Journal Title: J Bacteriol 19 Pages: 2392-2400
• [Presentation] 染色体ワークショップ (2008)
  • Author(s): 篠原美紀
  • Place of Presentation: ウエルサンピア姫路
• [Presentation] 日本分子生物学会年会 (2008)
  • Author(s): 篠原美紀
  • Place of Presentation: 神戸国際会議場
• [Presentation] 日本放射線影響学会 (2008)
  • Author(s): 篠原美紀
  • Place of Presentation: 北九州国際会議場
• [Presentation] 3R Symposium (2008)
  • Author(s): Miki Shinohara
  • Place of Presentation: 静岡県つま恋
• [Presentation] 酵母遺伝学フォーラム (2008)
  • Author(s): 篠原美紀
  • Place of Presentation: 北海道大学
• [Presentation] XX International Congress of Genetics (2008)
  • Author(s): Miki Shinohara
  • Place of Presentation: Berlin, Germany
• [Presentation] EMBO Workshop (2008)
  • Author(s): Miki Shinohara
  • Place of Presentation: Il Ciocco, Italy
• [Presentation] FHA domain of yeast Xrs2 promotes non-homologous end joining through the interaction with a Ligase-IV partner protein. Lifl (2008)
  • Author(s): Shinohara M
  • Organizer: EMBO Workshop
  • Place of Presentation: II Ciocco, Italy
• [Presentation] Crossover Assurance and Crossover Interference are Distinctly Regulated by the ZMM/SIC Proteins during Meiosis (2008)
  • Author(s): Shinohara M
  • Organizer: Genetics-Understanding Living Systems XX Intemational Congress of Genetics
  • Place of Presentation: Berlin, Germany
• [Presentation] 減数分裂期交叉型組換えの制御機構の解明 (2008)
  • Author(s): 篠原美紀
  • Organizer: 酵母遺伝学フォーラム
  • Place of Presentation: 北海道大学
• [Presentation] Molecular functions of ZMM/SIC complex during meiosis in Saccharomyces cerevisiae (2008)
  • Author(s): Shinohara M
  • Organizer: 3R Symposium
  • Place of Presentation: つま恋
• [Presentation] 非相同末端結合における酵母Xrs2の機能と細胞周期による制御 (2008)
  • Author(s): 篠原美紀
  • Organizer: 日本放射線影響学会
  • Place of Presentation: 北九州国際会議場
• [Presentation] Molecular mechanism of regulation in meiotic crossover formation (2008)
  • Author(s): 篠原美紀
  • Organizer: 日本分子生物学会年会
  • Place of Presentation: 神戸国際会議場
• [Presentation] 減数分裂期組換えにおけるATM/ATRキナーゼの機能 (2008)
  • Author(s): 篠原美紀
  • Organizer: 染色体ワークショップ
  • Place of Presentation: ウエルサンピア姫路
• [Presentation] 日本分子生物学会第30回年会 (2007)
  • Author(s): 篠原美紀
  • Place of Presentation: 横浜国際会議場
  • Year and Date: 2007-12-13
• [Presentation] 減数分裂期の組換えにおけるシナプトネマ複合体の機能解析Functional analysis of Synaptnemal complex in meioticrecombination (2007)
  • Author(s): 篠原美紀
  • Organizer: 日本分子生物学会
  • Place of Presentation: 横浜国際会議場
  • Year and Date: 2007-12-13
• [Presentation] InternationalSymposium on "Chromosome andGenome Stability & Instability" (2007)
  • Author(s): Miki Shinohara
  • Place of Presentation: Osaka
  • Year and Date: 2007-11-08
• [Presentation] FHA domain of yeast Xrs2 promotes non-homologous end joining through the interaction with a Ligase-IV partner protein, Lifl (2007)
  • Author(s): Miki Shinohara
  • Organizer: International Symposium on "Chromosome and Genome Stability & Instability"
  • Place of Presentation: 千里ライフサイエンスセンター
  • Year and Date: 2007-11-08
• [Presentation] 日本遺伝学会第79回大会 (2007)
  • Author(s): 篠原美紀
  • Place of Presentation: 岡山大学
  • Year and Date: 2007-09-13
• [Presentation] The 8th European Meiosis Meeting (2007)
  • Author(s): Miki Shinohara
  • Place of Presentation: Kanagawa
  • Year and Date: 2007-09-13
• [Presentation] Molecular functions of ZMM/SIC complex during meiosis in Sacchar omyces cerevisiae (2007)
  • Author(s): Miki Shinohara
  • Organizer: The 8th European Meiosis Meeting
  • Place of Presentation: Shonan Village Center, Kanagawa, Japan.
  • Year and Date: 2007-09-13
• [Presentation] シナプトネマ複合体の構成因子の減数分裂期相同組換え制御機構 (2007)
  • Author(s): 篠原美紀
  • Organizer: 日本遺伝学会 第79回大会
  • Place of Presentation: 岡山大学
  • Year and Date: 2007-09-13
• [Presentation] 酵母遺伝学フォーラム (2007)
  • Author(s): 篠原美紀
  • Place of Presentation: 大阪大学
  • Year and Date: 2007-09-11
• [Presentation] Liflのリン酸化による非相同末端結合制御機構 (2007)
  • Author(s): 篠原美紀
  • Organizer: 酵母遺伝学フォーラム
  • Place of Presentation: 大阪大学
  • Year and Date: 2007-09-11
• [Presentation] FHA domain of yeast Xrs2 promotes non-homologous end joining through the interaction with a Ligase-IV partner protein, Lifl (2007)
  • Author(s): K. Matsuzaki
  • Organizer: FASEB summer conference
  • Place of Presentation: Snowmass Village, Colorado
  • Year and Date: 2007-07-28
• [Presentation] 第23回染色体ワークショップ (2007)
  • Author(s): 小坂宏道、唐津
• [Presentation] The 20^<th> IUBMB International Congress ofBiochemistry and MolecularBiology (2006)
  • Author(s): Miki Shinohara, Kyoto
  • Year and Date: 2006-07-22
• [Presentation] 組換えワークショップ (2006)
  • Author(s): 篠原美紀
  • Place of Presentation: 淡路島夢舞台国際会議場
• [Presentation] 日本遺伝学会第78回大会 (2006)
  • Author(s): 篠原美紀, つくば
• [Presentation] 酵母遺伝学フォーラム第39回大会 (2006)
  • Author(s): 篠原美紀、三島
• [Remarks]
  • URL: http://www.protein.osaka-u.ac.jp/