Molecular dissection of the flowering process from induction to morphogenesis in Arabidopsis
Project/Area Number |
18370018
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
植物生理・分子
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Research Institution | Kyoto University |
Principal Investigator |
ARAKI Takashi Kyoto University, Graduate School of Biostidies, Professor (00273433)
|
Project Period (FY) |
2006 – 2007
|
Project Status |
Completed (Fiscal Year 2007)
|
Budget Amount *help |
¥15,340,000 (Direct Cost: ¥13,600,000、Indirect Cost: ¥1,740,000)
Fiscal Year 2007: ¥7,540,000 (Direct Cost: ¥5,800,000、Indirect Cost: ¥1,740,000)
Fiscal Year 2006: ¥7,800,000 (Direct Cost: ¥7,800,000)
|
Keywords | Higher plants / Flowering / Photoperiodism / Protein / mRNA / Signal transduction / Long-distance signal / Arabidopsis / シグナル伝達 |
Research Abstract |
Molecular events in FD-dependent and FD-independent flowering pathways and long-distance action of FT gene products (mRNA, protein) have been investigated. As components of the FD-dependent pathway, several Ca-dependent protein kinases (CDPKs) which may phosphorylate a C-terminal CDPK site in FD in the shoot apex were identified as FD-interactors by yeast two-hybrid assays. Biochemical demonstration of phosphorylation of FD will be the important next step in the future research. Genetic interaction between fd mutation and other flowering-related mutations was analyzed in detail and papers are currently being prepared. As an important component of the FD-independent flowering pathway, SOC1 was identified as an immediate downstream target of FT in the shoot apex. Several TCP transcription factors were identified as FT-interactors by yeast two-hybrid assays and bi-molecular fluorescent complementation assays. The role of these TCPs in flowering is currently being investigated. The long-distance action of FT in promotion of flowering was demonstrated using a local transient induction system for FT and two-shoot Y-grafting of young seedlings. The transport of FT-T7 protein from a single leaf blade to the shot apex or from a donor scion to the shoot apex of a recipient stock plants within 24-48 hours was observed. By using a synthetic FT gene with extensive synonymous substitution of all the codons in a single ORF, it was clearly shown that the mRNA sequence/structure per se is not of vital importance for the long-distance action of FT. These results have led to a conclusion that the FT protein, not mRNA, is the component of florigen.
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Report
(3 results)
Research Products
(79 results)
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[Journal Article] Morphological variation in leaf shape in Ainsliaea apiculata with special reference to the endemic characters of populations on Yakushima Island, Japan.2007
Author(s)
Tsukaya, H., Tsujino, R., Ikeuchi, M., Isshiki, Y., Kono, M., Takeuchi, T., Araki, T.
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Journal Title
Journal of Plant Research 120(印刷中)
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[Journal Article] Molecular basis of late-flowering phenotype caused by dominant epi-alleles of the FWA locus in Arabidopsis.2007
Author(s)
Ikeda, Y., Kobayashi, Y., Yamaguchi, A., Abe, M., Araki, T.
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Journal Title
Plant & Cell Physiology 48・2, 4
NAID
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[Journal Article] Increased frequency of homologous recombination and T-DNA integration in Arabidopsis CAF-1 mutants.2006
Author(s)
Endo, M., Ishikawa, Y., Osakabe, K., Nakayama, S., Kaya, H., Araki, T.ほか6名
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Journal Title
EMBO Journal 25・23
Pages: 5579-5590
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