FUNCTIONAL ANALYSIS OF PHOSPHO ENOLPYRUVATE CARBOXYLASE TARGETED TO THE CHLOROPLAST

• Project/Area Number: 18370022
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: 植物生理・分子
• Research Institution: National Institute of Agrobiological Sciences
• Principal Investigator: TOKUTOMI Mitsue National Institute of Agrobiological Sciences, 光環境応答研究ユニット, ユニット長 (70181980)
• Co-Investigator(Kenkyū-buntansha): FUKAYAMA Hiroshi 神戸大学, 大学院・農学研究科, 助教 (60373255)
• Research Collaborator: MASUMOTO Chisato 独立行政法人農業生物資源研究所, 光環境応答研究ユニット, 特別研究員 (60462561) ; MIYAZAWA Shin-ichi 独立行政法人農業生物資源研究所, 光環境応答研究ユニット, 特別研究員 (10578438)
• Project Period (FY): 2006 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥17,290,000 (Direct Cost: ¥14,500,000、Indirect Cost: ¥2,790,000); Fiscal Year 2008: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000); Fiscal Year 2007: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000); Fiscal Year 2006: ¥5,200,000 (Direct Cost: ¥5,200,000)
• Keywords: ホスホエノールピルビン酸カルボキシラーゼ / 葉緑体 / 有機酸 / 窒素同化 / イネ / 解糖系

Research Abstract

イネ特有の代謝酵素である葉緑体局在性ホスホエノールピルビン酸カルボキシラーゼ(PEPC)は、無機窒素を有機物に取り込む際必要な炭素化合物(有機酸)を作る働きをもつこと、イネはこの酵素が関与する独自の代謝経路をもち、この経路が水田のような湛水環境での無機窒素の利用に重要な役割を担うことが明らかにされた。葉緑体局在性PEPCは、光合成で固定された炭素の代謝と無機窒素の取り込みという、生産性に関わるふたつの過程をつなぐ鍵酵素であり、本酵素の関与する代謝経路の全容を理解することによって、イネの生産性の決定要因が明らかにされるものと期待される。

Research Products

• [Journal Article] Phosphoenolpyruvate carboxylase intrinsically located in the chloroplast of rice plays a crucial role in ammonium assimilation (2010)
  • Author(s): Masumoto, C., Miyazawa, S., Ohkawa, H., Fukuda, T., Taniguchi, Y., Murayama, S., Saito, K., Kusano, M., Fukavama, H., Miyao, M
  • Journal Title: Proc. Natl. Acad. Sci. USA 107 Pages: 5226-5231
  • Peer Reviewed
• [Journal Article] Gene expression profiling in leaves of rice grown under elevated CO2 (2009)
  • Author(s): Fukayama, H., Fukuda, T., Masumoto, C., Taniguchi, Y., Sakai, H., Cheng, W., Hasegawa, T., Miyao, M
  • Journal Title: Plant Sci 177 Pages: 203-210
  • Peer Reviewed
• [Journal Article] Overproduction of C_4 photosynthetic enzymes in transgenic rice plants : an approach to introduce the C_4-like photosynthetic pathway into rice (2008)
  • Author(s): Taniguchi, Y, Ohkawa, H., Masumoto, C., Fukuda, T., Tamai, T., Lee, K., Sudoh, S., Tsuchida, H., Sasaki, H., Fukayama, H., Miyao. M
  • Journal Title: J Exp. Bot 59 Pages: 1799-1809
  • Peer Reviewed
• [Journal Article] Overproduction of C_4 photosynthetic enzymes in transgenic rice plants:an approach to introduce the C_4-like photosthetic pathway into rice (2008)
  • Author(s): Taniguchi, Y.
  • Journal Title: Journal of Experimental Botany (Published on line)
  • Peer Reviewed
• [Journal Article] Characterization and functional analysis of phosphoenolpyruvate carboxylase kinase genes in rice (2006)
  • Author(s): Fukavama, H., Tamai, T., Taniguchi, Y., Sullivan, S., Miyao, M., Nimmo, H.G
  • Journal Title: Plant J 47 Pages: 258-268
  • Peer Reviewed
• [Journal Article] Identification of C_4 responsive genes in the facultative C_4 plant Hydrilla verticillata (2006)
  • Author(s): Rao, S.K., Fukavama, H., Reiskind, J., Miyao, M., Bowes, G
  • Journal Title: Photosynth. Res 88 Pages: 173-183
  • Peer Reviewed
• [Journal Article] Characterization and functional analysis of phosphoenolpyruvate carboxylase kinase genes in rice (2006)
  • Author(s): Fukayama, H., Tamai, T., Taniguchi, Y., Sullivan, S., Miyao, M., Nimmo,H.G.
  • Journal Title: The Plant Journal 47 Pages: 258-268
• [Journal Article] Identification of C_4 responsive genes in the facultative C_4 plant Hydrilla verticillata (2006)
  • Author(s): Rao, S.K., Fukayama, H., Reiskind, J., Miyao, M., Bowes,G.
  • Journal Title: Photosynthesis Research 88 Pages: 173-183
• [Presentation] 窒素同化におけるイネ葉緑体型ホスホエノールピルビン酸カルボキシラーゼ (PEPC) の役割 (2010)
  • Author(s): 増本千都, 宮澤真一, 宮尾光恵
  • Organizer: 第51回日本植物生理学会年会
  • Place of Presentation: 熊本
  • Year and Date: 2010-03-21
• [Presentation] イネ葉身のアンモニア同化における細胞質型と葉緑体型PEPCの役割 (2010)
  • Author(s): 宮澤真一, 増本千都, 宮尾光恵
  • Organizer: 第51回日本植物生理学会年会
  • Place of Presentation: 熊本
  • Year and Date: 2010-03-21
• [Presentation] 葉緑体型PEPC発現抑制イネの生理解析 (2009)
  • Author(s): 増本千都, 宮澤真一, 福田琢哉, 宮尾光恵
  • Organizer: 日本作物学会第228回講演会
  • Place of Presentation: 静岡
  • Year and Date: 2009-09-30
• [Presentation] phosphoenolpyruvate carboxylase of rice plays an important role in the nitrogen assimilation (2009)
  • Author(s): Masumoto, C., Miyazawa, S., Fukuda, T., Miyao, Chloroplastic
  • Organizer: Plant Biology 2009
  • Place of Presentation: ホノルル
• [Presentation] 4種類のC4光合成関連酵素を高発現する形質転換イネの生理解析 (2008)
  • Author(s): 増本千都, 谷口洋二郎, 福田琢哉, 大河浩, 佐々木治人, 深山浩, 宮尾光恵
  • Organizer: 日本作物学会第226回講演会
  • Place of Presentation: 神戸
  • Year and Date: 2008-09-25
• [Presentation] Metabolic consequences of transgenic rice overproducing four C_4 enzymes (2008)
  • Author(s): Miyao, M
  • Organizer: 2008 Gordon Research Conference on "CO_2 Assimilation in Plants : Genome to Biome,"
  • Place of Presentation: ビデフォード (米国)
  • Year and Date: 2008-08-21
• [Presentation] Metabolic consequences of transgenic rice overproducing four C_4 enzymes (2008)
  • Author(s): Miyao, M.
  • Organizer: 2008 Gordon Research Conference on“CO_2 Assimilation in Plants : Genome to Biome"
  • Place of Presentation: Biddeford (USA)
  • Year and Date: 2008-08-21
• [Presentation] Phosphoenolpyruvate carboxylase (PEPC) targeted to the chloroplast of rice (2008)
  • Author(s): Masumoto, C., Ohkawa, H., Taniguchi, Y., Fukuda, T., Fukayama, H., Miyao, M
  • Organizer: 2008 Gordon Research Conference on "CO_2 Assimilation in Plants Genome to Biome,"
  • Place of Presentation: ビデフォード(米国)
  • Year and Date: 2008-08-19
• [Presentation] Phosphoenolpyruvate carboxylase (PEPC) targeted to the chloroplast of rice (2008)
  • Author(s): Masumoto, C.
  • Organizer: 2008 Gordon Research Conference on“CO_2 Assimilation in Plants : Genome to Biome"
  • Place of Presentation: Biddeford (USA)
  • Year and Date: 2008-08-19
• [Presentation] イネ葉緑体型PEPCの発現様式と機能の解析 (2008)
  • Author(s): 増本千都, 大河浩, 谷口洋二郎, 福田琢哉, 深山浩, 徳富(宮尾)光恵
  • Organizer: 第49回日本植物生理学会年会
  • Place of Presentation: 札幌
  • Year and Date: 2008-03-20
• [Presentation] Anovel phosphoenolpyruvate carboxylase targeted to the chloroplast of rice (2007)
  • Author(s): Masumoto, C., Ohkawa, H., Taniguchi, Y., Fukuda, T., Fukayama, H., Miyao, M
  • Organizer: 14th International Congress of Photosynthesis
  • Place of Presentation: グラスゴー
  • Year and Date: 2007-07-23
• [Presentation] A novel phosphoellolpyruvate carboxylase targeted to the chloroplast of rice (2007)
  • Author(s): Masumoto, C.
  • Organizer: 14th International Congress of Photosynthesis
  • Place of Presentation: Glasgow
  • Year and Date: 2007-07-23
• [Presentation] Introduction of C_4-like photosynthetic pathway into rice : possibilities and limitations (2007)
  • Author(s): Miyao, M., Taniguchi, Y., Fukuda, T., Masumoto, C., Ohkawa, H., Fukayama, H., Sasaki, H
  • Organizer: C4 and CAM : from molecular diversity to ecological convergence
  • Place of Presentation: ケンブリッジ
  • Year and Date: 2007-07-19
• [Presentation] Introduction of C_4-like photosynthetic pathway into rice:possibilities and limitations (2007)
  • Author(s): Miyao, M.
  • Organizer: C4 and CAM:from molecular diversity to ecological convergence
  • Place of Presentation: Cambridge
  • Year and Date: 2007-07-19
• [Presentation] ゲノムから見た炭素代謝関連遺伝子 (2006)
  • Author(s): 徳富(宮尾)光恵, 大河浩, 谷口洋二郎, 深山浩
  • Organizer: 日本植物学会第70回大会
  • Place of Presentation: 熊本
  • Year and Date: 2006-09-30
• [Remarks] 雑誌論文(1), 2010年3月24日
  • URL: http://f100Obiology.com/article/id/2478959
• [Remarks] 窒素の有効利用のためのイネ独自のしくみを発見-イネの生産性向上に期待-, 2010年3月25日
  • URL: http://www.nias.affrc.go.jp/press/20100325/