Unraveling molecular mechanism of microtubule organization by analyses of microtubule branching factor

• Project/Area Number: 18370026
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Morphology/Structure
• Research Institution: National Institute for Basic Biology
• Principal Investigator: MURATA Takashi National Institute for Basic Biology, 生物進化研究部門, 准教授 (00242024)
• Co-Investigator(Kenkyū-buntansha): HASEBE Mitsuyasu 基礎生物学研究所, 生物進化研究部門, 教授 (40237996) ; HIWATASHI Yuji 基礎生物学研究所, 生物進化研究部門, 助教 (10373193)
• Project Period (FY): 2006 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥16,620,000 (Direct Cost: ¥15,300,000、Indirect Cost: ¥1,320,000); Fiscal Year 2008: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000); Fiscal Year 2007: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000); Fiscal Year 2006: ¥10,900,000 (Direct Cost: ¥10,900,000)
• Keywords: 微小管 / 植物細胞 / 細胞質分裂 / 隔膜形成体 / 細胞分裂 / 細胞板 / γチューブリン

Research Abstract

植物細胞においては、微小管の秩序だった構築は細胞の伸びる方向の制御や細胞分裂における細胞板形成に働く。微小管は中心体から生じると考えられているが植物細胞は中心体を持たないので、どのようにして微小管を構築するのかは謎だった。私は2005年に植物細胞の微小管が既存の微小管の上から枝状に伸び出すことを見いだし、本研究を開始した。本研究では細胞板形成における微小管の形成機構とその役割を調べ、微小管の枝分かれが細胞板の発達に働くことを明らかにした。また、その後の微小管の再構築に働く遺伝子を明らかにした。

Research Products

• [Journal Article] Kinesins are indispensable for interdigitation of phragmoplast microtubules in the moss Physcomitrella patens. (2008)
  • Author(s): Hiwatashi, Y., Obara, M., Sato, Y., Fujita, T., Murata, T., Hasebe, M.
  • Journal Title: Plant Cell 20 Pages: 3094-3106
  • Peer Reviewed
• [Journal Article] Kinesins are indispensable for interdigitation of phragmoplast microtubules in the moss Physcomitrella patens (2008)
  • Author(s): Hiwatashi, Y
  • Journal Title: Plant Cell 20 Pages: 3094-3106
  • Peer Reviewed
• [Journal Article] How do plants organize microtubules without a centrosome ? (2007)
  • Author(s): Murata, T., Tanahashi, T., Nishiyama, T., Yamaguchi, K., Hasebe, M.
  • Journal Title: J. Integrat. Plant Biol. 49 Pages: 1154-1163
  • Peer Reviewed
• [Journal Article] Microtubule-dependent microtubule nucleation in plant cells. (2007)
  • Author(s): Murata, T., Hasebe, M.
  • Journal Title: J. Plant Res. 120 Pages: 73-78
  • NAID: 10018828614
  • Peer Reviewed
• [Presentation] Three-demensional tracking of growing microtubule ends by two-photon microscopy. (2008)
  • Author(s): Murata, T., Nonaka, S., Sano, T., Hasezawa, S., Hasebe, M.
  • Organizer: 48th Annual Meeting of American Society for Cell Biology
  • Place of Presentation: San Francisco, U.S.A.
  • Year and Date: 2008-12-16
• [Presentation] Three-demensional tracking of growing microtubule ends by two-photon microscopy (2008)
  • Author(s): Takashi Murata
  • Organizer: 48th Annual meeting of the American Society for Cell Biology
  • Place of Presentation: San Francisco, U.S.A.
  • Year and Date: 2008-12-16
• [Presentation] 細胞板型細胞質分裂の分子機構 (2008)
  • Author(s): 村田 隆
  • Organizer: 日本分子生物学会第31回年会
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-10
• [Presentation] 細胞板型細胞質分裂の分子機構 (2008)
  • Author(s): 村田隆
  • Organizer: 日本分子生物学会第31回年会
  • Place of Presentation: 神戸ポートピアホテル
  • Year and Date: 2008-12-10
• [Presentation] 微小管構築のライブイメージ解析 (2008)
  • Author(s): 村田 隆
  • Organizer: 日本植物生理学会2008年度年会
  • Place of Presentation: 札幌
  • Year and Date: 2008-03-20
• [Presentation] Branched microtubules drive plant cytokinesis. (2007)
  • Author(s): Murata, T., Sano, T., Hasezawa, S., Hasebe, M.
  • Organizer: 47th Annual Meeting of American Society for Cell Biology
  • Place of Presentation: Wasington, D. C., U.S.A.
  • Year and Date: 2007-12-05
• [Presentation] Branched microtubules drive plant cytokinesis (2007)
  • Author(s): Takashi Murata
  • Organizer: American Society for Cell Biology
  • Place of Presentation: Washington,D.C.,U.S.A.
  • Year and Date: 2007-12-05
• [Presentation] 細胞質分裂機構の新しいモデル:モデルの再検討と検証 (2007)
  • Author(s): 村田 隆、長谷部光泰
  • Organizer: 日本植物学会第71回年会
  • Place of Presentation: 野田
  • Year and Date: 2007-09-07
• [Presentation] Roles of microtubule nucleation and reorganization in cell plate expansion at cytokinesis. (2007)
  • Author(s): Murata, T., Sano, T., Hasezawa, S., Hasebe, M.
  • Organizer: 第59回日本細胞生物学会大会
  • Place of Presentation: 福岡
  • Year and Date: 2007-05-29
• [Presentation] 細胞質分裂を制御する2型ユビキチン様タンパク質PUBLsの作用機作 (2007)
  • Author(s): 日渡 祐二, 小原 真理, 藤田 知道, 村田 隆, 長谷部 光泰
  • Organizer: 日本植物生理学会2007年度年会
  • Place of Presentation: 松山
  • Year and Date: 2007-03-29
• [Presentation] 微小管の動態解析から見えてきた細胞質分裂の分子機構 (2007)
  • Author(s): 村田 隆、佐野敏夫、馳澤盛一郎、長谷部光泰
  • Organizer: 日本植物生理学会2007年度年会
  • Place of Presentation: 松山
  • Year and Date: 2007-03-28
• [Book] Tobacco BY-2 Cells : From Cellular Dynamics to Omics (ed. by T. Nagata, K. Matsuoka, and D. Inze). Section I.3. Formation of cortical microtubules in a cell-free system prepared from plasma membrane ghosts and a cytosolic extract of BY-2 cells.(分担執筆) (2006)
  • Author(s): Murata, T., Hasebe, M.