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Genetic map-based analysis of the hybrid zone between Pinus pumila and Pinus parviflora var. Pentaphylla

Research Project

Project/Area Number 18370033
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Biodiversity/Systematics
Research InstitutionChiba University

Principal Investigator

WATANO Yasuyuki  Chiba University, 大学院・理学系研究科, 教授 (70192820)

Co-Investigator(Kenkyū-buntansha) 梶田 忠  千葉大学, 大学院・理学研究科, 准教授 (80301117)
朝川 毅守  千葉大学, 大学院・理学研究科, 助教 (50213682)
津村 義彦  独立行政法人森林総合研究所, 森林遺伝領域, 室長 (20353774)
Co-Investigator(Renkei-kenkyūsha) KAJITA Tadashi  千葉大学, 大学院・理学研究科, 准教授 (80301117)
ASAKAWA Takeshi  千葉大学, 大学院・理学研究科, 助教 (50213682)
TSUMURA Yoshihiko  独立行政法人森林総合研究所, 森林遺伝研究領域, 室長 (20353774)
Project Period (FY) 2006 – 2009
Project Status Completed (Fiscal Year 2009)
Budget Amount *help
¥17,020,000 (Direct Cost: ¥14,500,000、Indirect Cost: ¥2,520,000)
Fiscal Year 2009: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Fiscal Year 2008: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2007: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2006: ¥6,100,000 (Direct Cost: ¥6,100,000)
Keywordsゲノム / 連鎖地図 / 浸透性交雑 / ハイマツ / キタゴヨウ / グラフィカル・ジェノタイプ / EST / AFLP / 多様性 / SSCP
Research Abstract

Genetic linkage map of a hybrid between Pinus pumila and P . parviflora var. pentaphylla was constructed in order to examine the pattern of introgressive hybridization between the two pine species. Haploid megagametophytes from 96 seeds of a putative F1 parent were analyzed with EST, SSR and AFLP markers. The analysis resulted in 483 loci (64 ESTs, 2 SSRs and 417 AFLPs) mapping to 13 linkage groups under the criteria of LOD4.2 and θ<0.4.

Report

(6 results)
  • 2009 Annual Research Report   Final Research Report ( PDF )
  • 2008 Annual Research Report   Self-evaluation Report ( PDF )
  • 2007 Annual Research Report
  • 2006 Annual Research Report
  • Research Products

    (13 results)

All 2009 2008 2007

All Journal Article (6 results) (of which Peer Reviewed: 4 results) Presentation (7 results)

  • [Journal Article] Incongruence among mitochondrial, chloroplast and nuclear gene trees in Pinus subgenus Strobus (Pinaceae)2009

    • Author(s)
      Tsutsui, K., A. Suwa, K. Sawada, T. Kato, T. A. Ohsawa, Y. Watano
    • Journal Title

      Journal of Plant Research 122

      Pages: 509-521

    • NAID

      10025865542

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Incongruence among mitochondrial chloroplast and nuclear gene trees in Pinus subgenus Strobus(Pinaceae)2009

    • Author(s)
      Tsutsui, Suwa, Sawada, Kato, Ohsawa, Watano
    • Journal Title

      Journal of Plant Reserch 122

      Pages: 509-521

    • NAID

      10025865542

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Airborne-pollen pool and mating pattern in a hybrid zone between Pinus pumila and P. parviflora var. pentaphylla2008

    • Author(s)
      Ito, M., Y. Suyama, T. A. Ohsawa, Y. Watano
    • Journal Title

      Molecular Ecology 17

      Pages: 5092-5103

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Airborne-pollen pool and mating pattern in a hybrid zone between Pinus pumila and P. parviflora var. pentaphylla2008

    • Author(s)
      ITO M, SUYAMA Y, OHSAWA TA, WATANO Y
    • Journal Title

      Molecular Ecology 17

      Pages: 5092-5103

    • Related Report
      2008 Self-evaluation Report
  • [Journal Article] Airborne-pollen pool and mating pattern in a hybrid zone between Pinus pumla and P. parvifiora var. pentaphylla2008

    • Author(s)
      ITO M, SUYAMA Y, OHSAWA TA, WATANO Y
    • Journal Title

      Molecular Ecology 17

      Pages: 5092-5103

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Independent origins of tetraploid cryptic species in the fern Ceratopteris thalictroides2007

    • Author(s)
      ADJIE, B., S.MASUYAMA, H.ISHIKAWA, Y.WATANO
    • Journal Title

      Journal of Plant Research 120

      Pages: 129-138

    • NAID

      10018828993

    • Related Report
      2006 Annual Research Report
  • [Presentation] Hybrid zones between Pinus pumila and P. parviflora var. pentaphylla, a model system for studying introgressive hybridization of tree species.2009

    • Author(s)
      Watano, Y
    • Organizer
      Syposium on East Asian Plant Diversity and Conservation 2009
    • Place of Presentation
      CAS, Beijing, China
    • Year and Date
      2009-09-22
    • Related Report
      2009 Annual Research Report
  • [Presentation] Hybrid zones between Pinus pumila and P. parviflora var. pentaphylla, a model system for studying introgressive hybridization of tree species2009

    • Author(s)
      Watano, Y.
    • Organizer
      Syposium on East Asian Plant Diversity and Conservation 2009
    • Place of Presentation
      Beijing, China
    • Related Report
      2009 Final Research Report
  • [Presentation] ハイマツとキタゴヨウの雑種を用いた連鎖地図作成とグラフィカル・ジェノタイプ解析2009

    • Author(s)
      江夏知美・谷尚樹・津村義彦・朝川毅守・梶田忠・綿野泰行
    • Organizer
      日本植物分類学会
    • Place of Presentation
      宮城県民会館
    • Related Report
      2009 Final Research Report 2008 Annual Research Report
  • [Presentation] ハイマツとキタゴヨウの雑種を用いた連鎖地図作成とグラフィカル・ジェノタイプ解析2009

    • Author(s)
      江夏知美, 谷尚樹, 津村義彦, 朝川毅守, 梶田忠, 綿野泰行
    • Organizer
      日本植物分類学会
    • Related Report
      2008 Self-evaluation Report
  • [Presentation] ハイマツ・キタゴヨウ間の自然雑種のAFLPとESTマーカーを用いた連鎖地図作成2008

    • Author(s)
      江夏知美, 谷尚樹, 津村義彦, 朝川毅守, 梶田忠, 綿野泰行
    • Organizer
      日本植物学会
    • Year and Date
      2008-09-26
    • Related Report
      2008 Self-evaluation Report
  • [Presentation] ハイマツ・キタゴヨウ間の自然雑種のAFLPとESTマーカーを用いた連鎖地図作成2008

    • Author(s)
      江夏知美・谷尚樹・津村義彦・朝川毅守・梶田忠・綿野泰行
    • Organizer
      日本植物学会
    • Place of Presentation
      高知大学朝倉キャンパス
    • Year and Date
      2008-09-26
    • Related Report
      2008 Annual Research Report
  • [Presentation] ハイマツ・キタゴヨウ間の自然雑種のAFLPとESTマーカーを用いた連鎖地図作成2008

    • Author(s)
      江夏知美・谷尚樹・津村義彦・朝川毅守・梶田忠・綿野泰行
    • Organizer
      日本植物学会
    • Place of Presentation
      高知大学
    • Related Report
      2009 Final Research Report

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Published: 2006-04-01   Modified: 2016-04-21  

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