Study of the molecular genetic basis on evolution of multicellular body-plan in plants

• Project/Area Number: 18370034
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Biodiversity/Systematics
• Research Institution: The University of Tokyo
• Principal Investigator: ITO Motomi The University of Tokyo, 大学院・総合文化研究科, 教授 (00193524)
• Co-Investigator(Kenkyū-buntansha): SAKAYAMA Toshihide 東京大学, 大学院・総合文化研究科, 助教 (60391108) ; SEKIMOTO Hiroyuki 日本女子大, 理学部, 准教授 (20281652)
• Project Period (FY): 2006 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥17,640,000 (Direct Cost: ¥15,000,000、Indirect Cost: ¥2,640,000); Fiscal Year 2008: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000); Fiscal Year 2007: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000); Fiscal Year 2006: ¥6,200,000 (Direct Cost: ¥6,200,000)
• Keywords: 遺伝子 / 植物 / 進化 / ゲノム / ミカヅキモ / シャジクモ

Research Abstract

本研究は、被子植物へと繋がる植物の系統での多様性創生に関わるもっとも重要なステップである多細胞化について着目し、その分子遺伝学的基盤を明らかにすることが目的である。そのため、単細胞生物であるミカヅキモと多細胞化したシャジクモにおいて、遺伝子の網羅的かつ系統的解析を行い、どのような制御機構や遺伝子ネットワークが多細胞化や初期の器官分化において重要な働きをしたかを明らかにする。
シャジクモの栄養成長時の細胞と有性生殖器官からそれぞれRNA を単離し, cDNA を合成した.両者のcDNA について均一化したライブラリーを作製してEST を取得した.約6000 個について配列決定を行い、プロファイリングを行なった。転写制御遺伝子であるLEAFY 遺伝子とKNOX遺伝子について行ない、シャジクモ藻類で初めて単離に成功した。 これらの遺伝子についてDNA 配列決定を行ない、陸上植物の相同遺伝子とともに系統解析を行なうとともに発現解析を行った。

Research Products

• [Journal Article] Taxonomic reexamination of Chara globularis (Charales, Charophyceae) from Japan based on oospore morphology and rbcL gene sequences, and the description of C (2009)
  • Author(s): Sakayama, H., Kasai, F., Kawachi, M., Watanabe, M. M., Nozaki, H., Nishihiro, J., Washitani, I., Shigyo, M., Krienitz, L. & Ito, M.
  • Journal Title: leptospora sp. nov. Journal of Phycology (In press)
• [Journal Article] Expression of the exogenous genes under the control of the endogenous HSP70 and CAB promoters in Closterium peracerosum-strigosum-littorale complex (2008)
  • Author(s): Abe,, J. Hiwatashi, Y., Ito, M., Hasebe, M., Sekimoto, H.
  • Journal Title: Plant Cell Physiol 49 Pages: 625-632
  • NAID: 10027330567
• [Journal Article] Convergent evolution of shoots in land plants : lack of auxin polar transport in moss shoots (2008)
  • Author(s): Fujita, T., Sakaguchi, H, Hiwatashi, Y., Wagstaff, S.J., Ito, M., Deguchi, H., Sato, T. and Hasebe, M.
  • Journal Title: Evol. Develop 10:2 Pages: 176-186
• [Journal Article] Taxonomy of Nitella (Charales, Charophyceae) based on comparative morphology of oospores and multiple DNA marker phylogeny using cultured material (2008)
  • Author(s): Sakayama, H.
  • Journal Title: Phycological Research 56 Pages: 202-215
• [Journal Article] Morphological variation and intraspecific phylogeny of the ubiquitous species Chara braunii (Charales, Charophyceae)(in Japan) (2008)
  • Author(s): Kato, S., Sakayama, H., Sano, S., Kasai, F., Watanabe, M. M., Tanaka, J. & Nozaki, H.
  • Journal Title: Phycologia 47 Pages: 191-202
• [Journal Article] Expression of the exogenous genes under the control of the endogenous HSP70 and CAB promoters in Closterium peracerosum-strigosum-littorale complex. (2008)
  • Author(s): Abe., J. Hiwatashi, Y., Ito, M., Hasebe, M., Sekimoto, H.
  • Journal Title: Plant Cell Physiol. 49 Pages: 623-632
  • NAID: 10027330567
  • Peer Reviewed
• [Journal Article] The sporophyte-less filmy fern of eastern North America Trichomanes intricatum (Hymenophyllaceae) has the chloroplast genome of an Asian species. (2008)
  • Author(s): Ebihara, A., Farrar, D.R., and Ito, M.
  • Journal Title: Amer. J. Bot. 95 Pages: 1645-1651
  • Peer Reviewed
• [Journal Article] Expression of exogenous genes under the control of endogenous HSP70 and CAB promoters in the Closterium peracerosum-strigosum-littorale complex (2008)
  • Author(s): Abe, , J. Hiwatashi, Y., Ito, M., Hasebe, M., Sekimoto, H.
  • Journal Title: Plant Cell Physiol 49 Pages: 625-632
  • NAID: 10027330567
  • Peer Reviewed
• [Journal Article] Convergent evolution of shoots in land plants:lack of auxin polar transport in moss shoots (2008)
  • Author(s): Fujita, T., Sakaguchi, H, Hiwatashi, Y., Wagstaff, S.J., Ito, M., Deguchi, H., Sato, T., and Hasebe, M.
  • Journal Title: Evolution and Development 10 Pages: 176-186
  • Peer Reviewed
• [Journal Article] A Sex Pheromone,Protoplast Release-inducihg Protein(PR-IP)Inducer,Induces Sexual Cell Division and Production of PR-IP in Closterium (2008)
  • Author(s): Tsuchikane, Y., Fujii., T., Ito, M., Hasebe, M., Sekimoto, H.
  • Journal Title: Plant Cell Physiol 49 Pages: 1472-1476
  • Peer Reviewed
• [Journal Article] Seasonality of gametophyte occurrence, maturation and fertilization of the freshwater red alga Thorea okadae (Thoreales, Rhodophyta) in the Kikuchi River (2007)
  • Author(s): Higa, A., Kasai, F., Kawachi, M., Kumano, S., Sakayama, H., Miyashita, M. & Watanabe, M. M.
  • Journal Title: Japan. Phycologia 46 Pages: 160-167
• [Journal Article] Gene expression profiling using cDNA microarray analysis of the sexual reproduction stage of the unicellular Charophycean alga Closterium peracerosumstrigosum-littorale Complex (2006)
  • Author(s): Sekimoto, H., Tanabe, Y., Tsuchikane, Y., Shirosaki, H., Fukuda, H., Demura, T. and Ito, M.
  • Journal Title: Plant Physiol 141 Pages: 271-279
• [Journal Article] Sex allocation bias in hermaphroditic plants : effects of local competition and seed dormancy (2006)
  • Author(s): Tsuchimatsu, T., Sakai, S. and Ito, M.
  • Journal Title: Evol. Ecol. Res 8 Pages: 829-842
  • NAID: 120003728112
• [Journal Article] Isolation of myosin XI genes from the Closterium peracerosum-strigosum-littorale complex and analysis of their expression during sexual reproduction (2006)
  • Author(s): Hamada, S., Sekimoto, H., Tanabe, Y., Tsuchikane, Y., Ito, M.
  • Journal Title: J. Plant Res 119 Pages: 105-113
  • NAID: 10018126202
• [Journal Article] Isolation of myosin XI genes from the Closterium peracerosum-strigosum-littorale complex and analysis of their expression during sexual reproduction. (2006)
  • Author(s): Hamada, S., Sekimoto, H., Tanabe, Y., Tsuchikane, Y., Ito, M.
  • Journal Title: J. Plant Res. 119 Pages: 271-279
  • NAID: 10018126202
• [Journal Article] Gene expression profiling using cDNA microarray analysis of the sexual reproduction stage of the unicellular Charophycean alga Closterium peracerosum-strigosum-littorale Complex. (2006)
  • Author(s): Sekimoto, H., Tanabe, Y., Tsuchikane, Y., Shirosaki, H., Fukuda, H., Demura, T., Ito, M.
  • Journal Title: Plant Physiol. 141 Pages: 271-279
• [Presentation] シャジクモ類におけるLEAFY遺伝子ホモログの単離 (2009)
  • Author(s): 坂山英俊・伊藤元己
  • Organizer: 日本藻類学会
  • Place of Presentation: 那覇
  • Year and Date: 2009-03-28
• [Presentation] シャジクモ(Chara braunii)の生殖器官形成の解析 (2009)
  • Author(s): 佐藤真知子・坂山英俊・関本弘之・伊藤元己
  • Organizer: 日本藻類学会
  • Place of Presentation: 那覇
  • Year and Date: 2009-03-28
• [Presentation] シャジクモ類におけるLEAFY遺伝子ホモログの単離 (2009)
  • Author(s): 坂山英俊・伊藤元己
  • Organizer: 日本藻類学会第33回大会
  • Place of Presentation: 琉球大学
  • Year and Date: 2009-03-27
• [Presentation] 接藻ヒメミカヅキモの非対称な生殖隔離に関わる性フェロモン (2008)
  • Author(s): 土金勇樹・山崎華奈子・伊藤元己・関本弘之
  • Organizer: 日本藻類学会
  • Place of Presentation: 東京
  • Year and Date: 2008-03-22
• [Presentation] .ヒメミカヅキモへの外来遺伝子導入による形質転換体の作出 (2008)
  • Author(s): 堀早知恵・阿部淳・伊藤元己・関本弘之
  • Organizer: 日本藻類学会
  • Place of Presentation: 東京
  • Year and Date: 2008-03-22
• [Presentation] ヒメミカヅキモのKNOX遺伝子クローニングと分子的解析 (2007)
  • Author(s): 関本弘之・坂本季美枝・田邊陽一・阿部淳・伊藤元己
  • Organizer: 日本植物学会
  • Place of Presentation: 野田
  • Year and Date: 2007-09-05
• [Presentation] Molecular approaches for sexual reproduction of a unicellular charophycean alga, Closterium peracerosum-strigosum-littorale complex (2007)
  • Author(s): Sekimoto, H.
  • Organizer: Intemational Symposium on Biology and Taxonomy of Green Algae V
  • Place of Presentation: Smolenice, Slovakia
  • Year and Date: 2007-06-25