• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Study of the molecular genetic basis on evolution of multicellular body-plan in plants

Research Project

Project/Area Number 18370034
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Biodiversity/Systematics
Research InstitutionThe University of Tokyo

Principal Investigator

ITO Motomi  The University of Tokyo, 大学院・総合文化研究科, 教授 (00193524)

Co-Investigator(Kenkyū-buntansha) SAKAYAMA Toshihide  東京大学, 大学院・総合文化研究科, 助教 (60391108)
SEKIMOTO Hiroyuki  日本女子大, 理学部, 准教授 (20281652)
Project Period (FY) 2006 – 2008
Project Status Completed (Fiscal Year 2008)
Budget Amount *help
¥17,640,000 (Direct Cost: ¥15,000,000、Indirect Cost: ¥2,640,000)
Fiscal Year 2008: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2007: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2006: ¥6,200,000 (Direct Cost: ¥6,200,000)
Keywords遺伝子 / 植物 / 進化 / ゲノム / ミカヅキモ / シャジクモ
Research Abstract

本研究は、被子植物へと繋がる植物の系統での多様性創生に関わるもっとも重要なステップである多細胞化について着目し、その分子遺伝学的基盤を明らかにすることが目的である。そのため、単細胞生物であるミカヅキモと多細胞化したシャジクモにおいて、遺伝子の網羅的かつ系統的解析を行い、どのような制御機構や遺伝子ネットワークが多細胞化や初期の器官分化において重要な働きをしたかを明らかにする。
シャジクモの栄養成長時の細胞と有性生殖器官からそれぞれRNA を単離し, cDNA を合成した.両者のcDNA について均一化したライブラリーを作製してEST を取得した.約6000 個について配列決定を行い、プロファイリングを行なった。転写制御遺伝子であるLEAFY 遺伝子とKNOX遺伝子について行ない、シャジクモ藻類で初めて単離に成功した。 これらの遺伝子についてDNA 配列決定を行ない、陸上植物の相同遺伝子とともに系統解析を行なうとともに発現解析を行った。

Report

(4 results)
  • 2008 Annual Research Report   Final Research Report ( PDF )
  • 2007 Annual Research Report
  • 2006 Annual Research Report
  • Research Products

    (23 results)

All 2009 2008 2007 2006

All Journal Article (16 results) (of which Peer Reviewed: 5 results) Presentation (7 results)

  • [Journal Article] Taxonomic reexamination of Chara globularis (Charales, Charophyceae) from Japan based on oospore morphology and rbcL gene sequences, and the description of C2009

    • Author(s)
      Sakayama, H., Kasai, F., Kawachi, M., Watanabe, M. M., Nozaki, H., Nishihiro, J., Washitani, I., Shigyo, M., Krienitz, L. & Ito, M.
    • Journal Title

      leptospora sp. nov. Journal of Phycology (In press)

    • Related Report
      2008 Final Research Report
  • [Journal Article] Expression of the exogenous genes under the control of the endogenous HSP70 and CAB promoters in Closterium peracerosum-strigosum-littorale complex2008

    • Author(s)
      Abe,, J. Hiwatashi, Y., Ito, M., Hasebe, M., Sekimoto, H.
    • Journal Title

      Plant Cell Physiol 49

      Pages: 625-632

    • NAID

      10027330567

    • Related Report
      2008 Final Research Report
  • [Journal Article] Convergent evolution of shoots in land plants : lack of auxin polar transport in moss shoots2008

    • Author(s)
      Fujita, T., Sakaguchi, H, Hiwatashi, Y., Wagstaff, S.J., Ito, M., Deguchi, H., Sato, T. and Hasebe, M.
    • Journal Title

      Evol. Develop 10:2

      Pages: 176-186

    • Related Report
      2008 Final Research Report
  • [Journal Article] Taxonomy of Nitella (Charales, Charophyceae) based on comparative morphology of oospores and multiple DNA marker phylogeny using cultured material2008

    • Author(s)
      Sakayama, H.
    • Journal Title

      Phycological Research 56

      Pages: 202-215

    • Related Report
      2008 Final Research Report
  • [Journal Article] Morphological variation and intraspecific phylogeny of the ubiquitous species Chara braunii (Charales, Charophyceae)(in Japan)2008

    • Author(s)
      Kato, S., Sakayama, H., Sano, S., Kasai, F., Watanabe, M. M., Tanaka, J. & Nozaki, H.
    • Journal Title

      Phycologia 47

      Pages: 191-202

    • Related Report
      2008 Final Research Report
  • [Journal Article] Expression of the exogenous genes under the control of the endogenous HSP70 and CAB promoters in Closterium peracerosum-strigosum-littorale complex.2008

    • Author(s)
      Abe., J. Hiwatashi, Y., Ito, M., Hasebe, M., Sekimoto, H.
    • Journal Title

      Plant Cell Physiol. 49

      Pages: 623-632

    • NAID

      10027330567

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The sporophyte-less filmy fern of eastern North America Trichomanes intricatum (Hymenophyllaceae) has the chloroplast genome of an Asian species.2008

    • Author(s)
      Ebihara, A., Farrar, D.R., and Ito, M.
    • Journal Title

      Amer. J. Bot. 95

      Pages: 1645-1651

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Expression of exogenous genes under the control of endogenous HSP70 and CAB promoters in the Closterium peracerosum-strigosum-littorale complex2008

    • Author(s)
      Abe, , J. Hiwatashi, Y., Ito, M., Hasebe, M., Sekimoto, H.
    • Journal Title

      Plant Cell Physiol 49

      Pages: 625-632

    • NAID

      10027330567

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Convergent evolution of shoots in land plants:lack of auxin polar transport in moss shoots2008

    • Author(s)
      Fujita, T., Sakaguchi, H, Hiwatashi, Y., Wagstaff, S.J., Ito, M., Deguchi, H., Sato, T., and Hasebe, M.
    • Journal Title

      Evolution and Development 10

      Pages: 176-186

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A Sex Pheromone,Protoplast Release-inducihg Protein(PR-IP)Inducer,Induces Sexual Cell Division and Production of PR-IP in Closterium2008

    • Author(s)
      Tsuchikane, Y., Fujii., T., Ito, M., Hasebe, M., Sekimoto, H.
    • Journal Title

      Plant Cell Physiol 49

      Pages: 1472-1476

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Seasonality of gametophyte occurrence, maturation and fertilization of the freshwater red alga Thorea okadae (Thoreales, Rhodophyta) in the Kikuchi River2007

    • Author(s)
      Higa, A., Kasai, F., Kawachi, M., Kumano, S., Sakayama, H., Miyashita, M. & Watanabe, M. M.
    • Journal Title

      Japan. Phycologia 46

      Pages: 160-167

    • Related Report
      2008 Final Research Report
  • [Journal Article] Gene expression profiling using cDNA microarray analysis of the sexual reproduction stage of the unicellular Charophycean alga Closterium peracerosumstrigosum-littorale Complex2006

    • Author(s)
      Sekimoto, H., Tanabe, Y., Tsuchikane, Y., Shirosaki, H., Fukuda, H., Demura, T. and Ito, M.
    • Journal Title

      Plant Physiol 141

      Pages: 271-279

    • Related Report
      2008 Final Research Report
  • [Journal Article] Sex allocation bias in hermaphroditic plants : effects of local competition and seed dormancy2006

    • Author(s)
      Tsuchimatsu, T., Sakai, S. and Ito, M.
    • Journal Title

      Evol. Ecol. Res 8

      Pages: 829-842

    • NAID

      120003728112

    • Related Report
      2008 Final Research Report
  • [Journal Article] Isolation of myosin XI genes from the Closterium peracerosum-strigosum-littorale complex and analysis of their expression during sexual reproduction2006

    • Author(s)
      Hamada, S., Sekimoto, H., Tanabe, Y., Tsuchikane, Y., Ito, M.
    • Journal Title

      J. Plant Res 119

      Pages: 105-113

    • NAID

      10018126202

    • Related Report
      2008 Final Research Report
  • [Journal Article] Isolation of myosin XI genes from the Closterium peracerosum-strigosum-littorale complex and analysis of their expression during sexual reproduction.2006

    • Author(s)
      Hamada, S., Sekimoto, H., Tanabe, Y., Tsuchikane, Y., Ito, M.
    • Journal Title

      J. Plant Res. 119

      Pages: 271-279

    • NAID

      10018126202

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Gene expression profiling using cDNA microarray analysis of the sexual reproduction stage of the unicellular Charophycean alga Closterium peracerosum-strigosum-littorale Complex.2006

    • Author(s)
      Sekimoto, H., Tanabe, Y., Tsuchikane, Y., Shirosaki, H., Fukuda, H., Demura, T., Ito, M.
    • Journal Title

      Plant Physiol. 141

      Pages: 271-279

    • Related Report
      2006 Annual Research Report
  • [Presentation] シャジクモ類におけるLEAFY遺伝子ホモログの単離2009

    • Author(s)
      坂山英俊・伊藤元己
    • Organizer
      日本藻類学会
    • Place of Presentation
      那覇
    • Year and Date
      2009-03-28
    • Related Report
      2008 Final Research Report
  • [Presentation] シャジクモ(Chara braunii)の生殖器官形成の解析2009

    • Author(s)
      佐藤真知子・坂山英俊・関本弘之・伊藤元己
    • Organizer
      日本藻類学会
    • Place of Presentation
      那覇
    • Year and Date
      2009-03-28
    • Related Report
      2008 Final Research Report
  • [Presentation] シャジクモ類におけるLEAFY遺伝子ホモログの単離2009

    • Author(s)
      坂山英俊・伊藤元己
    • Organizer
      日本藻類学会第33回大会
    • Place of Presentation
      琉球大学
    • Year and Date
      2009-03-27
    • Related Report
      2008 Annual Research Report
  • [Presentation] 接藻ヒメミカヅキモの非対称な生殖隔離に関わる性フェロモン2008

    • Author(s)
      土金勇樹・山崎華奈子・伊藤元己・関本弘之
    • Organizer
      日本藻類学会
    • Place of Presentation
      東京
    • Year and Date
      2008-03-22
    • Related Report
      2008 Final Research Report
  • [Presentation] .ヒメミカヅキモへの外来遺伝子導入による形質転換体の作出2008

    • Author(s)
      堀早知恵・阿部淳・伊藤元己・関本弘之
    • Organizer
      日本藻類学会
    • Place of Presentation
      東京
    • Year and Date
      2008-03-22
    • Related Report
      2008 Final Research Report
  • [Presentation] ヒメミカヅキモのKNOX遺伝子クローニングと分子的解析2007

    • Author(s)
      関本弘之・坂本季美枝・田邊陽一・阿部淳・伊藤元己
    • Organizer
      日本植物学会
    • Place of Presentation
      野田
    • Year and Date
      2007-09-05
    • Related Report
      2008 Final Research Report
  • [Presentation] Molecular approaches for sexual reproduction of a unicellular charophycean alga, Closterium peracerosum-strigosum-littorale complex2007

    • Author(s)
      Sekimoto, H.
    • Organizer
      Intemational Symposium on Biology and Taxonomy of Green Algae V
    • Place of Presentation
      Smolenice, Slovakia
    • Year and Date
      2007-06-25
    • Related Report
      2007 Annual Research Report

URL: 

Published: 2006-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi