Mechanism of Biogenesis and Catalytic Function of Peptidyl Built-in Quinone Cofactors
Project/Area Number |
18370043
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Structural biochemistry
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Research Institution | Osaka University |
Principal Investigator |
TANIZAWA Katsuyuki Osaka University, 産業科学研究所, 教授 (20133134)
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Co-Investigator(Kenkyū-buntansha) |
OKAJIMA Toshihide 大阪大学, 産業科学研究所, 准教授 (10247968)
|
Project Period (FY) |
2006 – 2009
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Project Status |
Completed (Fiscal Year 2009)
|
Budget Amount *help |
¥17,390,000 (Direct Cost: ¥15,500,000、Indirect Cost: ¥1,890,000)
Fiscal Year 2009: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2008: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Fiscal Year 2007: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2006: ¥9,200,000 (Direct Cost: ¥9,200,000)
|
Keywords | 銅アミン酸化酵素 / トパキノン / ビルトイン型キノン補酵素 / キノヘムプロテイン / 分子内チオエーテル架橋 / 翻訳後修飾 / X線結晶解析 / 酵素触媒機構 / 分子内チオエ-テル架橋 / 酵素解媒機構 / ラジカル・SAMタンパク質 |
Research Abstract |
Peptidyl built-in cofactors are produced by posttranslational modification of the cognate enzyme proteins, unlike ordinary cofactors which are mostly bio-synthesized from water soluble vitamins. In this study, we focused on two built-in quinone cofactor-containing enzymes, copper amine oxidase and quinohemoprotein amine dehydrogenase (QHNDH) that contain topa quinone (TPQ) and cysteine tryptophyl quinone (CTQ), respectively. For the reaction mechanism of copper amine oxidase, it has been demonstrated that the former reductive half-reaction is accompanied with the conformational change of TPQ. On the other hand, two proteins encoded close to the QHNDH genes, an [Fe-S]-cluster and S-adenosylmethionine-binding protein and a subtilisin-like protease, have been found to play essential roles for QHNDH biogenesis.
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Report
(6 results)
Research Products
(60 results)
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[Journal Article]2009
Author(s)
Suzuki, S., Okajima, T., Tanizawa, K., Mure, M.(共蓍)
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Journal Title
Copper Amine Oxidases : Structures, Catalytic Mechanisms, and Role in Pathophysiology Cofactors of Amine Oxidases : Copper Ion and Its Substitution and the 2, 4, 5-Trihydoxylphenylalanine Quinone(Chapter3)(CRC Press, Taylor & francis Group)
Related Report
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[Journal Article] Further Insight into the Mechanism of Stereoselective Proton Abstraction by Bacterial Copper Amine Oxidase.2008
Author(s)
Taki, M., Murakawa, T., Nakamoto, T., Uchida, M., Hayashi, H., Tanizawa, K., Yamamoto, Y., Okajima, T.
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Journal Title
Biochemistry 47
Pages: 7726-7733
Related Report
Peer Reviewed
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[Journal Article] Further Insight into the Mechanism of Stereoselective Proton Abstraction by Bacterial Copper Amine Oxidase2008
Author(s)
Taki, M., Murakawa, T., Nakamoto, T., Uchida, M., Hayashi, H., Tanizawa, K., Yamamoto, Y., Okajima, T.
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Journal Title
Biochemistry 47
Pages: 7726-7733
Related Report
Peer Reviewed
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[Journal Article] Further Insight into the Mechanism of Stereoselective Proton Abstraction by Bacterial Copper Amine Oxidase.2008
Author(s)
Taki, M., Murakawa, T., Nakamoto, T., Uchida, M., Hayashi, H., Tanizawa, K., Yamamoto, Y., Okajima, T.
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Journal Title
Biochemistry 47(29)
Pages: 7726-7733
Related Report
Peer Reviewed
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[Journal Article] Trapping of a Dopaquinone Intermediate in the TPQ Cofactor Biogenesis in a Copper-containing Amine Oxidase from Arthrobacter globiformis.2007
Author(s)
Moore, R.H., Spies, M.A., Culpepper, M.B., Murakawa, T., Hirota, S., Okajima, T., Tanizawa K., Mure, M.
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Journal Title
J. Am. Chem. Soc. 129
Pages: 11524-11534
Related Report
Peer Reviewed
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[Journal Article] Trapping of a Dopaquinone Intermediate in the TPQ Cofactor Biogenesis in a Copper-containing Amine Oxidase from Arthrobacter globiformis2007
Author(s)
Moore, R. H., Spies, M. A., Culpepper, M. B., Murakawa, T., Hirota, S., Okajima, T., Tanizawa K., Mure, M.
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Journal Title
J. Am. Chem. Soc. 129
Pages: 11524-11534
Related Report
Peer Reviewed
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[Journal Article] Trapping of a Dopaquinone Intermediate in the TPQ Cofactor Biogenesis in a Copper-containing Amine Oxidase from Arthrobacter globiformis.2007
Author(s)
Moore, R. H., Spies, M. A., Culpepper, M.B., Murakawa, T., Hirota, S., Okajima, T., Tanizawa K., and Mure M.
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Journal Title
J. Am, Chem. Soc. 129
Pages: 11524-11534
Related Report
Peer Reviewed
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[Journal Article] nvolvement of a Putative [Fe-S]-Cluster-Binding Protein in the Biogenesis of Quinohemoprotein Amine Dehydrogenase.2006
Author(s)
Ono, K., Okajima, T., Tani, M., Kuroda, S., Sun, D., Davidson, V.L., Tanizawa, K.
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Journal Title
J. Biol. Chem. 281
Pages: 13672-13684
Related Report
Peer Reviewed
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[Journal Article] Kinetic and Structural Studies on the Catalytic Role of the Aspartic Acid Residue Conserved in Copper Amine Oxidase.2006
Author(s)
Chiu, Y.-C., Okajima, T., Murakawa, T., Uchida, M., Taki, M., Hirota, S., Kim, M., Hayashi, H., Yamamoto, Y., Tanizawa, K.
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Journal Title
Biochemistry 45
Pages: 4105-4120
Related Report
Peer Reviewed
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[Journal Article] Quantum Mechanical Hydrogen Tunneling in Bacterial Copper Amine Oxidase Reaction. Biochem.2006
Author(s)
Murakawa, T., Okajima, T., Kuroda, S., Nakamoto, T., Taki, M., Yamamoto, Y., Hayashi, H., Tanizawa, K.
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Journal Title
Biophys. Res. Commun. 342
Pages: 414-423
Related Report
Peer Reviewed
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[Journal Article] Involvement of a Putative [Fe-S]-Cluster-Binding Protein in the Biogenesis of Quinohemoprotein Amine Dehydrogenase2006
Author(s)
Ono, K., Okajima, T., Tani, M., Kuroda, S., Sun, D., Davidson, V.L., Tanizawa, K.
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Journal Title
J. Biol. Chem. 281
Pages: 13672-13684
Related Report
Peer Reviewed
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[Journal Article] Kinetic and Structural Studies on the Catalytic Role of the Aspartic Acid Residue Conserved in Copper Amine Oxidase2006
Author(s)
Chiu, Y.-C., Okajima, T., Murakawa, T., Uchida, M., Taki, M., Hirota, S., Kim, M., Hayashi, H., Yamamoto, Y., Tanizawa, K.
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Journal Title
Biochemistry 45
Pages: 4105-4120
Related Report
Peer Reviewed
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[Journal Article] Kinetic and Structural Studies on the Catalytic Role of the Aspartic Acid Residue Conserved in Copper Amine Oxidase2006
Author(s)
Yen-Chen Chiu, Toshihide Okajima, Takeshi Murakawa, Mayumi Uchida, Masayasu Taki, Shun Hirota, Misa Kim, Hiroshi Yamaguchi, Yoshiaki Kawano, Nobuo Kamiya, Shun'ichi Kuroda, Hideyuki Hayashi, Yukio Yamamoto, Katsuyuki Tanizawa
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Journal Title
Biochemistry 45・13
Pages: 4105-4120
Related Report
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[Presentation] X-ray Crystallographic Evidence for Conformational Change of Topaquinone in the Reductive Half-reaction of Copper Amine Oxidase from Arthrobacter globiformis, Gordon Research Conference on Protein Cofactors2010
Author(s)
Hamaguchi, A., Okajima, T., Nakai, T. Tanizawa, K., Murakawa, T., Hayashi, H.
Organizer
Radicals and Quinones
Place of Presentation
Ventura, CA, USA
Related Report
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[Presentation] Acid-Base Chemistry in the Reductive Half-Reaction of Copper Amine Oxidase from Arthrobacter globiformis2010
Author(s)
Murakawa, T., Hayashi, H., Okajima, T., Tanizawa, K.
Organizer
Gordon Research Conference on Protein Cofactors, Radicals and Quinones
Place of Presentation
Ventura, CA, USA
Related Report
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[Presentation] Acid-Base Chemistry in the Reductive Half-Reaction of Copper Amine Oxidase from Arthrobacter globiformis2010
Author(s)
Murakawa, T., Hayashi, H., Okajima, T., Tanizawa, K.
Organizer
Gordon Research Conference on 'Protein Cofactors, Radicals and Quinones'
Place of Presentation
Four Points Sheraton/Holiday Inn Express, Ventura, CA, USA
Related Report
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[Presentation] X-ray Crystallographic Evidence for Conformational Change of Topaquinone in the Reductive Half-reaction of Copper Amine Oxidase from Arthrobacter globiformis2010
Author(s)
Hamaguchi, A., Okajima, T., Nakai, T., Tanizawa, K., Murakawa, T., Hayashi, H.
Organizer
Gordon Research Conference on 'Protein Cofactors, Radicals and Quinones'
Place of Presentation
Four Points Sheraton/Holiday Inn Express, Ventura, CA, USA
Related Report
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[Presentation] Conformational Flexibility of the TPQ Cofactor in Bacterial Copper Amine Oxidase, Gordon Research Conference on Protein Cofactors2008
Author(s)
Okajima, T., Nakanishi, S., Murakawa, T., Hayashi, H., Tanizawa, K.
Organizer
Radicals and Quinones
Place of Presentation
Ventura, CA, USA
Related Report
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[Book] Cofactors of Amine Oxidases: Copper Ion and Its Substitution and the 2, 4, 5-Trihydoxylphenylalanine Quinone (Chapter 3). In'Copper Amine Oxidases: Structures, Catalytic Mechanisms, and Role in Pathophysiology'(Taylor & Francis Group)2009
Author(s)
Suzuki, S., Okajima, T., Tanizawa, K., Mure, M.
Publisher
CRC Press
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