Structure-function correlation of disease-related functional RNA

• Project/Area Number: 18370046
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Structural biochemistry
• Research Institution: Yokohama City University
• Principal Investigator: KATAHIRA Masato Yokohama City University, International Graduate School of Arts and Sciences, Professor (70211844)
• Project Period (FY): 2006 – 2007
• Project Status: Completed (Fiscal Year 2007)
• Budget Amount: ¥15,320,000 (Direct Cost: ¥14,000,000、Indirect Cost: ¥1,320,000); Fiscal Year 2007: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000); Fiscal Year 2006: ¥9,600,000 (Direct Cost: ¥9,600,000)
• Keywords: multi-drug resistance / anti-cancer dru / aptamer / Rev / 4'-thioDNA / Vault / C型肝炎 / 構造 / IRES / 薬剤耐性 / NMR / RNA

Research Abstract

Vault particle is related to multi-drug resistance. We found that RNA component of the vault (vault RNA)binds to anti-cancer drug, mitoxantrone. On the basis of chemical shift perturbation with NMR, we found that vault RNA binds to several other anti-cancer drugs. The binding site for drugs was also identified. By blocking the binding site with its complementary oligonucleotide, the trap of drugs by vault. RNA may be suppressed, which could lead to the overcome of the multi-drug resistance.
The aptamer composed of RNA containing the Rev-responsive element and the Rev peptide was developed by our collaborator, Professor Morii of Kyoto University. We analyzed the structure of this aptamer in complex with its ligand. The formation of new hydrogen bonds was observed for RNA upon the binding of the Rev peptide and the ligand, respectively. The secondary structure of RNA was deduced. The structure determination of the aptamer is in progress.
It was reported that DNA composed of the 4'-thiodeoxyriboses, 4'-thioDNA, exhibits remarkable features. Particularly, 4'-thioDNA is resistant to cleavage by DNase I. We determined the structure of 4'-thioDNA by NMR. DNA usually takes on B-form. 4'-thioDNA, however, takes on A-form which is the form for RNA. DNase I binds to DNA through the interaction at the narrow and relatively deep minor groove of the B-form. The minor groove of 4'-thioDNA is wide and shallow, which is characteristic to A-form, and is not suitable for the binding of DNase I. The other remarkable features of 4'-thioDNA were also interpreted on the basis of the determined structure.

Research Products

• [Journal Article] Unexpected A-form formation of 4'-thioDNA insolution,revealed by NMR,and the implications as to the mechanism of nuclease-resistance (2008)
  • Author(s): Matsugami, A., Ohyama, T., Inada, M., moue, N., Minakawa, N., Matsuda, A., Katahira, M.
  • Journal Title: Nucleic Acids Res 36 Pages: 1805-1812
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Unexpected A-form formation of 4'-thioDNA in solution, revealed by NMR, and the implications as to the mechanism of nuclease-resistance (2008)
  • Author(s): Matsugami, A., Ohyama, T., Inada, M., Inoue, N., Minakawa, N., Matsuda, A., Katahira, M
  • Journal Title: Nucleic Acids Res 36 Pages: 1805-1812
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Unexpected A-form formation of 4'-thioDNA in solution, revealed by NMR, and the implications as to the mechanism of nuclease-resistance (2008)
  • Author(s): Matsugami, A., Ohyama, T., Inada, M., Inoue, N., Minakawa, N., Matsuda, A., Katahira, M.
  • Journal Title: Nucleic Acids Res. 36 Pages: 1805-1812
  • Peer Reviewed
• [Journal Article] Structure of human telomeric DNA under physiological ionic conditions stabilized by proper incorporation of 8-bromoguanosines,as deternuned by NMR (2007)
  • Author(s): Matsugami, M., Xu, Y., Noguchi, Y., Sugiyama, H. and Katahira, M.
  • Journal Title: FEBS J. 274 Pages: 3545-3556
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Structural analysis of the fundamental polymer of the sheath constructed by Sphaerotilus natans (2007)
  • Author(s): Takeda., M., Miyanoiri, Y., Nogami, T., Oda, K., Saito, T., Kato, K., Koizumi, J.I., Katahira, M.
  • Journal Title: Biosci.Biotechnol.Biochem 71 Pages: 2992-2998
  • NAID: 10027522051
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Interactions with RNA/DNA of proteins involved in the regulation of transcription,translation and telomere elongation (2007)
  • Author(s): Ohyama, T., Furukawa, A., Miyoshi, T., Takada, Y., OhgaraS., Hiratsuka, K., Ixnai, T., Okano, H., Nakagama, H., Nagata, T. and Katahira, M.
  • Journal Title: Nucleic Acids Symp.Ser No.51 Pages: 77-78
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Structure of 4'-thioDNA which exhibits endonuclease resistance (2007)
  • Author(s): Matsugami A., Ohyama, T., Inada, M., moue, N., Minakawa, N., Matsuda, A. and Katahira, M.
  • Journal Title: Nucleic Acids Symp.Ser No.51 Pages: 141-142
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Structure of human telomeric DNA under physiological ionic conditions stabilized by proper incorporation of 8-bromoguanosines, as determined by NMR (2007)
  • Author(s): Matsugami, M., Xu, Y., Noguchi, Y., Sugiyama, H., Katahira, M
  • Journal Title: FEBS J 274 Pages: 3545-3556
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Structural analysis of the fundamental polymer of the sheath constructed by Sphaerotilus natans (2007)
  • Author(s): Takeda, M., Miyanoiri, Y., Nogami, T., Oda, K., Saito, T., Kato, K., Koizumi, J. I., Katahira, M
  • Journal Title: Biosci. Biotechnol. Biochem 71 Pages: 2992-2998
  • NAID: 10027522051
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Interactions with RNA/DNA of proteins involved in the regulation of transcription, translation and telomere elongation (2007)
  • Author(s): Ohyama, T., Furukawa, A., Miyoshi, T., Takada, Y., Ohgara, S., Hiratsuka, K., Imai, T., Okano, H., Nakagama, H., Nagata, T., Katahira, M
  • Journal Title: Nucleic Acids Symp. Ser 51 Pages: 77-78
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Structure of 4'-thioDNA which exhibits endonuclease resistance (2007)
  • Author(s): Matsugami, A., Ohyama, T., Inada, M., Inoue, N., Minakawa, N., Matsuda, A., Katahira, M
  • Journal Title: Nucleic Acids Symp. Ser 51 Pages: 141-142
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Structure of human telomeric DNA under physiological ionic conditions stabilized by proper incorporation of 8-bromoguanosines, as determined by NMR (2007)
  • Author(s): Matsugami, M., Xu, Y., Noguchi, Y., Sugiyama, H. and Katahira, M.
  • Journal Title: FEBS J. 274 Pages: 3545-3556
  • Peer Reviewed
• [Journal Article] Structural analysis of the fundamental polymer of the sheath constructed by Sphaerotilus natans (2007)
  • Author(s): Takeda, M., Miyanoiri, Y., Nogami, T., Oda, K., Saito, T., Kato, K., Koizumi, J. I., Katahira, M.
  • Journal Title: Biosci. Biotechnol. Biochem. 71 Pages: 2992-2998
  • NAID: 10027522051
  • Peer Reviewed
• [Journal Article] Interactions with RNA/DNA of proteins involved in the regulation of transcription, translation and telomere elongation (2007)
  • Author(s): Ohyama, T., Furukawa, A., Miyoshi, T., Takada, Y., Ohgara, S., Hiratsuka, K., Imai, T., Okano, H., Nakagama, H., Nagata, T. and Katahira, M.
  • Journal Title: Nucleic Acids Symp. Ser. 51 Pages: 77-78
  • Peer Reviewed
• [Journal Article] Structure of 4'-thioDNA which exhibits endonuclease resistance (2007)
  • Author(s): Matsugami A., Ohyama, T., Inada, M., Inoue, N., Minakawa, N., Matsuda, A. and Katahira, M.
  • Journal Title: Nucleic Acids Symp. Ser. 51 Pages: 141-142
  • Peer Reviewed
• [Journal Article] Structure of human telomeric DNA under physiological ionic conditions stabilized by proper incorporation of 8-bromoguanosines,as determined by NMR (2007)
  • Author(s): Matsugami, M他
  • Journal Title: FEBS J 印刷中
• [Journal Article] Molecular mechanism for maintenance of G-rich short tandem repeats capable of adopting G4 DNA structures (2006)
  • Author(s): Nakagama, H., Higuchi, K., Tanaka, E., Tsuchiya, N., Nakashima, K., Katahira, M. and Fukuda, H.
  • Journal Title: Mutat.Res 598 Pages: 120-131
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Structural property of DNA that migrates faster in gelelectrophoresis,as deduced by CD spectroscopy (2006)
  • Author(s): Matsugami, A., Tani, K., Ouhashi, K., Uesugi, S., Morita, M., Ohyama, T. and Katahira M.
  • Journal Title: Nucleosides, Nucleotides and Nucleic Acids 25 Pages: 417-425
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Identification of 2-(cysteinyl)amido-2-deoxy-D-galacturonic acidresidue from the sheath of Leptothrix cholodnii (2006)
  • Author(s): Makita, H., Nakahara, Y., Fukui, H., Miyanoiri, Y., Katahira, M., Seki, H., Takeda, M.and Koizumi, J.
  • Journal Title: Biosci.Biotech.& Biochem 70 Pages: 1265-1268
  • NAID: 10018531700
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Unichrom,a novel nuclear matrix protein,binds to the Ars insulator and canonical MARs (2006)
  • Author(s): Tagashira, H., Shimotori, T., Sakamoto, N., Katahira, M., Miyanoiri, Y., Yamamoto, T., Mitsunaga-Nakatsubo K., Kusunoki, S.and Akasaka, K.
  • Journal Title: Zoological Science 23 Pages: 9-21
  • NAID: 110004775619
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Structural analysis of DNA containing(6-4)photoproduct in complex with distamycin A by NMR (2006)
  • Author(s): Tsuchibayashi, H., Ohyama, T., Matsugami, A., Nanjo, T., Iwai, S. and Katahira, M.
  • Journal Title: Nucleic Acids Symp.Ser No.50 Pages: 237-238
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] The new models of the human telomere DNA in K^+ solution revealed by NMR analysis assisted by the incorporation of 8-bromoguanines (2006)
  • Author(s): Matsugami, A., Tsuchibayashi, H., Xu, Y., Noguchi, Y., Sugiyama, H. and Katahira, M.
  • Journal Title: Nucleic Acids Symp.Ser No.50 Pages: 42-43
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Molecular mechanism for maintenance of G-rich short tandem repeats capable of adopting G4 DNA structures (2006)
  • Author(s): Nakagama, H., Higuchi, K., Tanaka, E., Tsuchiya, N., Nakashima, K., Katahira, M., Fukuda, H
  • Journal Title: Mutat. Res 598 Pages: 120-131
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Structural property of DNA that migrates faster in gel electrophoresis, as deduced by CD spectroscopy (2006)
  • Author(s): Matsugami, A., Tani, K., Ouhashi, K., Uesugi, S., Morita, M., Ohyama, T., Katahira, M
  • Journal Title: Nucleosides, Nucleotides and Nucleic Acids 25 Pages: 417-425
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Identification of 2-(cysteinyl)amido-2-deoxy-D-galacturonic acid residue from the sheath of Leptothrix cholodnii (2006)
  • Author(s): Makita, H., Nakahara, Y., Fukui, H., Miyanoiri, Y., Katahira, M., Seki, H., Takeda, M., Koizumi, J
  • Journal Title: Biosci. Biotech. & Biochem 70 Pages: 1265-1268
  • NAID: 10018531700
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Unichrom, a novel nuclear matrix protein, binds to the Ars insulator and canonical MARs (2006)
  • Author(s): Tagashira, H., Shimotori, T., Sakamoto, N., Katahira, M., Miyanoiri, Y., Yamamoto, T., Mitsunaga-Nakatsubo K., Kusunoki, S., Akasaka, K
  • Journal Title: Zoological Science 23 Pages: 9-21
  • NAID: 110004775619
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Structural analysis of DNA containing (6-4) photoproduct in complex with distamycin A by NMR (2006)
  • Author(s): Tsuchibayashi, H., Ohyama, T., Matsugami, A., Nanjo, T., Iwai, S., Katahira, M
  • Journal Title: Nucleic Acids Symp. Ser 50 Pages: 237-238
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] The new models of the human telomere DNA in K+ solution revealed by NMR analysis assisted by the incorporation of 8-bromoguanines (2006)
  • Author(s): Matsugami, A., Tsuchibayashi, H., Xu, Y., Noguchi, Y., Sugiyama, H., Katahira, M
  • Journal Title: Nucleic Acids Symp. Ser 50 Pages: 42-43
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Unichrom, a novel nuclear matrix protein, binds to the Ars insulator and canonical MARs (2006)
  • Author(s): Tagashira, H他
  • Journal Title: Zoological Science 23 Pages: 9-21
• [Journal Article] Structural property of DNA that migrates faster in gel electrophoresis,as deduced by CD spectroscopy (2006)
  • Author(s): Matsugami, A他
  • Journal Title: Nucleosides,Nucleotides and Nucleic Acids 25 Pages: 417-425
• [Journal Article] Identification of 2-(cysteinyl)amido-2-deoxy-D-galacturonic acid residue from the sheath of Leptothrix cholodnii (2006)
  • Author(s): Makita, H他
  • Journal Title: Biosci.Biotech. ＆ Biochem 70 Pages: 1265-1268
• [Journal Article] Molecular mechanism for maintenance of G-rich short tandem repeats capable of adopting G4 DNA structures (2006)
  • Author(s): Nakagama, H他
  • Journal Title: Mutat.Res 598 Pages: 120-131
• [Journal Article] NMRによるタンパク質構造解析の展望 (2006)
  • Author(s): 宮ノ入洋平他
  • Journal Title: 酵素工学ニュース 55 Pages: 29-34
• [Journal Article] Structural analysis of DNA containing (6-4) photoproduct in complex with distamycin A by NMR (2006)
  • Author(s): Tsuchibayashi, H他
  • Journal Title: Nucleic Acids Symp. Ser. 50 Pages: 237-238
• [Journal Article] The new models of the human telomere DNA in K^+ solution revealed by NMR analysis assisted by the incorporation of 8-bromoguanines (2006)
  • Author(s): Matsugami, A他
  • Journal Title: Nucleic Acids Symp. Ser 50 Pages: 42-43
• [Presentation] Structures and interactions of 4'-thioDNA and RNA/DNA-binding proteins (2008)
  • Author(s): Ohyama, T., Furukawa, A., Takada, Y., Ono, A., Matsugami, A., Mashima, T., Nagata, T.and Katahira, M.
  • Organizer: XXIII International Conference on Magnetic Resonance in Biological Systems
  • Place of Presentation: アメリカ
  • Year and Date: 2008-08-26
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Structure determination of amulti-domain protein by the use of PRE and RDC,and the unexpected A-form structure of 4'-thioDNA exhibiting nuclease-resistance (2008)
  • Author(s): Ohyama, T., Furukawa, A., Matsugami, A., Mashima, T., Nagata, T. and Katahira, M.
  • Organizer: Korea-Japan Symposiumon Biological NMR
  • Place of Presentation: 韓国
  • Year and Date: 2008-04-25
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Attempt to construct the ion channel composed of nucleic acids (2008)
  • Author(s): Miyoshi, T., Matsugami, A., Nomura, S., Akiyoshi, K.and Katahira M.
  • Organizer: International Conference on Membrane Interface
  • Place of Presentation: 博多
  • Year and Date: 2008-01-25
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Structure determination of a multi-domain protein by the use of PRE and RDC, and the unexpected A-form structure of 4'-thioDNA exhibiting nuclease-resistance (2008)
  • Author(s): Ohyama, T., Furukawa, A., Matsugami, A., Mashima, T., Nagata, T., Katahira, M
  • Organizer: Korea-Japan Symposium on Biological NMR
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Structures and interactions of 4'-thioDNA and RNA/DNA-binding proteins (2008)
  • Author(s): Ohyama, T., Furukawa, A., Takada, Y., Ono, A., Matsugami, A., Mashima, T., Nagata, T., Katahira, M
  • Organizer: XXIII International Conference on Magnetic Resonance in Biological Systems
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Attempt to construct the ion channel composed of nucleic acids (2008)
  • Author(s): Miyoshi, T., Matsugami, A., Nomura, S., Akiyoshi, K., Katahira M
  • Organizer: International Conference on Membrane Interface
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] 多剤耐性に関与するVault粒子のRNA成分と薬剤の相互作用の解析 (2007)
  • Author(s): 高田 祐輔, 松上 明正, 工藤 倫子, ゴピナス スバシュCB, クマール ペンメチャK.R, 片平 正人
  • Organizer: 第30回日本分子生物学会年会第80回日本生化学会大会 合同大会
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-11
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 多剤耐性に関与するVault粒子のRNA成分と薬剤の相互作用の解析 (2007)
  • Author(s): 真嶋司
  • Organizer: 第30回日本分子生物学会年会・第80回日本生化学会大会 合同大会
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-11
• [Presentation] 機能性核酸とタンパク質の構造生物学 (2007)
  • Author(s): 片平 正人
  • Organizer: 関西07講演会
  • Place of Presentation: 姫路
  • Year and Date: 2007-09-15
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] The structure of human telomeric DNA and the interactions of hnRNP A1/hnRNPD proteins with telomeric DNA (2007)
  • Author(s): Matsugami, A., Ohyama, T., Ono, A., Ono, M., Nagata, T.and Katahira, M.
  • Organizer: International Workshop on Perspectives on Stable Isotope Aided NMR Methods for Protein Structural Analvsis
  • Place of Presentation: 大阪
  • Year and Date: 2007-03-31
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] (Structural studies on transcriptional and post-transcriptional generegulation by NMR (2007)
  • Author(s): Nagata, T., Ohyama, T., Noto, K., Niyada, E., Miyanoiri, Y., Imai, T., Okano, H., Hiratsuka, K., Katahira, M.
  • Organizer: International Workshop on Perspectives on Stable Isotope Aided NMR Methods for Protein Structural Analysis
  • Place of Presentation: 大阪
  • Year and Date: 2007-03-30
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] The structure of human telomeric DNA and the interactions of hnRNP A1 /hnRNP D proteins with telomeric DNA (2007)
  • Author(s): Matsugami, A., Ohyama, T., Ono, A., Ono, M., Nagata, T., Katahira, M
  • Organizer: International Workshop on Perspectives on Stable Isotope Aided NMR Methods for Protein Structural Analysis
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Structural studies on transcriptional and post-transcriptional gene regulation by NMR (2007)
  • Author(s): Nagata, T., Ohyama, T., Noto, K., Niyada, E., Miyanoiri, Y., Imai, T., Okano, H., Hiratsuka, K., Katahira, M
  • Organizer: International Workshop on Perspectives on Stable Isotope Aided NMR Methods for Protein Structural Analysis
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] テロメア及びテロメア結合タンパク質の構造・機能とテロメア長の制御 (2006)
  • Author(s): 片平 正人
  • Organizer: NMR 2006
  • Place of Presentation: 東京
  • Year and Date: 2006-12-16
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] エンドヌクレアーゼ耐性を示す4'-thioDNAの構造解析 (2006)
  • Author(s): 稲田 雅士, 片平 正人
  • Organizer: 分子生物学フォーラム
  • Place of Presentation: 名古屋
  • Year and Date: 2006-12-07
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] HCV IRESを捕捉するRNAアプタマーの構造解析 (2006)
  • Author(s): 小泉 大輔, 片平 正人
  • Organizer: 分子生物学フォーラム
  • Place of Presentation: 名古屋
  • Year and Date: 2006-12-07
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Structural studies on the neural RNA-binding protein Musashil in complex with its cognate RNA by NMRi (2006)
  • Author(s): Ohyama, T., Nagata, T., Miyanoiri, Y., Imai, T., Okano, H. and Katahira, M.
  • Organizer: RNA 2006 Izu-Functional RNAs and Regulatory Machinery
  • Place of Presentation: 伊豆
  • Year and Date: 2006-12-05
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Structural Biology of Functional RNA and DNA (2006)
  • Author(s): 片平 正人
  • Organizer: 第45回NMR討論会
  • Place of Presentation: 京都
  • Year and Date: 2006-11-23
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Structural analysis of Musashi protein complexed with target RNA (2006)
  • Author(s): 大山 貴子, 片平 正人
  • Organizer: NMR討論会
  • Place of Presentation: 京都
  • Year and Date: 2006-11-23
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 8-プロモグアニンの導入にアシストされたNMR解析によって示されたヒトテロメアDNAのカリウム溶液中における新規モデル (2006)
  • Author(s): 松上 明正, 片平 正人
  • Organizer: 第33回核酸化学シンポジウム
  • Place of Presentation: 大阪
  • Year and Date: 2006-11-21
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] (6-4)光損傷部位を含む DNA とジスタマイシンAの複合体のNMRによる構造解析 (2006)
  • Author(s): 土林 寛昭, 片平 正人
  • Organizer: 第33回核酸化学シンポジウム
  • Place of Presentation: 大阪
  • Year and Date: 2006-11-20
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] タンパク質・DNA・RNA・リガンド問の相対配置・相互作用のNMR法による解析 (2006)
  • Author(s): 片平 正人
  • Organizer: 遺伝学研究セミナー
  • Place of Presentation: 三島
  • Year and Date: 2006-10-25
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Investigations of functional RNA/DNA and RNA/DNA-binding proteins (2006)
  • Author(s): Tsuchibayashi, H., Ohyama, T., Matsugami, A., Miyanoiri, Y., Niyada, E., Nagata, T.and Katahira.M.
  • Organizer: 4th Japan-Taiwan NMR Symposium
  • Place of Presentation: 台湾
  • Year and Date: 2006-10-16
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 病気の発症ないしは抑制に関連したタンパク質及び機能性核酸の構造生物学 (2006)
  • Author(s): 片平 正人
  • Organizer: よこはまNMR構造生物学研究会
  • Place of Presentation: 横浜
  • Year and Date: 2006-09-25
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Structures of Musashi in Complex with RNA,Wild and Mutant (Phosphorylation-Mimicking)DNA-Binding Proteins,GT-1,and Telomere DNA in K+ Solution (2006)
  • Author(s): Ohyama, T., Tsuchibayashi, H., Matsugami, A., Miyanoiri, Y., Niyada, E., Nagata, T.and Katahira, M.
  • Organizer: The Switzerland-Japan Symposium on Structural Biology 2006
  • Place of Presentation: スイス
  • Year and Date: 2006-09-18
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Structures of telomere DNA in K+ solution,Musashi in complex with RNA,and wild and mutant(phosphorylation-mimicking)DNA-binding proteins,GT-1 (2006)
  • Author(s): Ohyama, T., Tsuchibayashi, H., Matsugami, A., Miyanoiri, Y., Niyada, E., Nagata, T. and Katahira, M.
  • Organizer: XXII International Conference on Magnetic Resonance in Biological Systems
  • Place of Presentation: ドイツ
  • Year and Date: 2006-08-22
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 翻訳を抑制するタンパク質及びRNAに関する構造解析 (2006)
  • Author(s): 片平 正人
  • Organizer: 第8回RNAミーティング
  • Place of Presentation: 淡路
  • Year and Date: 2006-07-20
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Wild and mutant(phosphorylation-mimicking)GT-1structures,hnRNP D-telomere DNA complex structure,and Musashi structure complexed with RNA (2006)
  • Author(s): Ohyama, T., Tsuchibayashi, H., Matsugami, A., Miyanoiri, Y., Niyada, E., Enokizono, Y., Nagata, T.and Katahira, M.
  • Organizer: Korea-Japan Bilateral Symposium on Biological NMR
  • Place of Presentation: 韓国
  • Year and Date: 2006-07-08
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Investigations of functional RNA/DNA and RNA/DNA-binding proteins (2006)
  • Author(s): Tsuchibayashi, H., Ohyama, T., Matsugami, A., Miyanoiri, Y., Niyada, E., Nagata, T., Katahira, M
  • Organizer: 4th Japan-Taiwan NMR Symposium
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Structures of Musashi in Complex with RNA, Wild and Mutant (Phosphorylation-Mimicking) DNA-Binding Proteins, GT-1, and Telomere DNA in K+ Solution (2006)
  • Author(s): Ohyama, T., Tsuchibayashi, H., Matsugami, A., Miyanoiri, Y., Niyada, E., Nagata, T., Katahira, M
  • Organizer: The Switzerland-Japan Symposium on Structural Biology 2006
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Wild and mutant (phosphorylation-mimicking) GT-1 structures, hnRNP D-telomere DNA complex structure, and Musashi structure complexed with RNA (2006)
  • Author(s): Ohyama, T., Tsuchibayashi, H., Matsugami, A., Miyanoiri, Y., Niyada, E., Enokizono, Y., Nagata, T., Katahira, M
  • Organizer: Korea-Japan Bilateral Symposium on Biological NMR
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Structures of telomere DNA in K+ solution, Musashi in complex with RNA, and wild and mutant (phosphorylation-mimicking) DNA-binding proteins, GT-1 (2006)
  • Author(s): Ohyama, T., Tsuchibayashi, H., Matsugami, A., Miyanoiri, Y., Niyada, E., Nagata, T., Katahira, M
  • Organizer: XXII International Conference on Magnetic Resonance in Biological Systems
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Structural studies on the neural RNA-binding protein Musashil in complex with its cognate RNA by NMRi (2006)
  • Author(s): Ohyama, T., Nagata, T., Miyanoiri, Y., Imai, T., Okano, H., Katahira, M
  • Organizer: RNA 2006 Izu -Functional RNAs and Regulatory Machinery-
  • Description: 「研究成果報告書概要(欧文)」より
• [Book] RNA (2007)
  • Author(s): 片平 正人
  • Total Pages: 662
  • Publisher: 朝倉書店
  • Description: 「研究成果報告書概要(和文)」より
• [Book] 紫外吸収スペクトル (2007)
  • Author(s): 大山 貴子、松上 明正、片平 正人
  • Publisher: 羊土社
  • Description: 「研究成果報告書概要(和文)」より
• [Book] 生物物理学ハンドブック(分担) (2007)
  • Author(s): 片平正人
  • Publisher: 朝倉書店
• [Book] 生命科学のための機器分析実験ハンドブック(分担) (2007)
  • Author(s): 大山貴子、松上明正、片平正人
  • Publisher: 羊土社
• [Book] NMRの応用・生体高分子 (2006)
  • Author(s): 片平 正人
  • Publisher: 丸善
  • Description: 「研究成果報告書概要(和文)」より
• [Book] NMRによるタンパク質構造解析の展望-Musashiタンパク質によるRNAの認識機構の解析を例に (2006)
  • Author(s): 宮ノ入 洋平、片平 正人
  • Description: 「研究成果報告書概要(和文)」より
• [Book] 第5版 実験化学講座8NMR・ESR(分担) (2006)
  • Author(s): 片平正人
  • Publisher: 丸善