Physiological roles of CD47-SHPS-1 system, an intercellular signaling, in regulation of various cellular functions
| Project/Area Number |
18370054
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Functional biochemistry
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| Research Institution | Gunma University |
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Principal Investigator |
MATOZAKI Takashi Gunma University, IMCR, Professor (80252782)
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| Project Period (FY) |
2006 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥17,270,000 (Direct Cost: ¥15,500,000、Indirect Cost: ¥1,770,000)
Fiscal Year 2007: ¥7,670,000 (Direct Cost: ¥5,900,000、Indirect Cost: ¥1,770,000)
Fiscal Year 2006: ¥9,600,000 (Direct Cost: ¥9,600,000)
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| Keywords | Signal transduction / Biological molecules / Protein / Brain・Neuron / Immunology / 細胞間シグナル伝達 / SHPS-1 / CD47 / 神経突起伸長 / マクロファージ機能 / 赤血球 / 貪食 |
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| Research Abstract |
SHPS-1 is a transmembrane protein whose extracellular region interacts with CD47, a ligand for SHPS-1. The cytoplasmic region of SHPS-1 binds the protein tyrosine phosphatases SHIP-1 or SHP-2. CD47 is a penta-transmembrane protein, which was originally identified as a binding partner of integrin. We have recently elucidated that CD47 and SHIPS-1 constitute an intercellular communication system (the CD47-SHPS-1 system), that plays important roles in the following cell functions. In this study, we investigated the physiological roles of CD47-SHPS-1 system. The results obtained are follows: (1) Interaction of CD47 with SHIPS-1 cooperatively regulates neurite development and eventually it contributes to formtion of neuronal networks. (2) The cytoplasmic region as well as tyrosine phosphorylation sites in this region of SHIPS-1 appear indispensable for this inhibitory action of SHIPS-1 for macrophage phagocytosis. SHIPS-1 may regulate the attachment of phagocytosed target cells to macrophages. (3) Trans-interaction of CD47 and SHPS-1 that occurred on contact of CD47-expressing cells and SHIPS-1-expressing cells results in endocytosis of the ligand-receptor complex into either cell type (trans-endocytosis). Such endocytosis is regulated by clathrin or dynamin as well as Rae and Cdc42. (4) SHPS-1 expressed on dendritic cells is important for induction of Th17 or Th1 cells as well as activation of NKT cells.
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Report
(3 results)
Research Products
(63 results)
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[Presentation] Regulation by SHPS-1 of skin immunity and of Toll-like receptor-mediated cytokine production2007
Author(s)
Motegi, S., Okazawa, H., Kobayashi, H., Murata, Y., Ohnishi, H., Ishikawa, O., and Matozaki, T.
Organizer
The 37th annual meeting of the Japanese Society for Immunology
Place of Presentation
Tokyo
Year and Date
2007-11-20
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Presentation] Regulation by SHIPS-1 of skin immunity and of Toll-like receptor-mediated cytokine production2007
Author(s)
Motegi, S., Okazawa, H., Kobayashi, H., Murata, Y., Ohnishi, H., Ishikawa, O., and Matozaki, T.
Organizer
The 66th annual meeting of the Japanese Cancer Association
Place of Presentation
Yokohama
Year and Date
2007-10-03
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Presentation] Trans-endocytosis of CD47-SHPS-1 system and its molecular Mechanism2007
Author(s)
Kusakari, S., Ohnishi, H., Okazawa, H., Murata, T., Murata, Y., and Matozaki, T.
Organizer
The 66th annual meeting of the Japanese Cancer Association
Place of Presentation
Yokohama
Year and Date
2007-10-03
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Presentation] Trans-endocytosis of CD47 and SHIPS-1 and its molecular Mechanism2007
Author(s)
Ohnishi, H., Kusakari, S., Houketsu, K., Murata, T., Shinohara, M., Murata, Y., Okazawa, H., and Matozaki, T.
Organizer
Neuro2007
Place of Presentation
Yokohama
Year and Date
2007-09-10
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Presentation] Regulation by CD47-SHPS-1 system of neurite development and its molecular mechanism2007
Author(s)
Ohnishi, H., Murata, T., Miyashita, M., Kusakari, S., Okazawa, H., Murata, Y., Itoh, H., Furuya, N., and Matozaki, T.
Organizer
The 59th annual meeting of the Japan Society for Cell Biology
Place of Presentation
Fukuoka
Year and Date
2007-05-28
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Presentation] Regulation by Src homology 2 domain-containing protein tyrosine phosphatase substrate-1 of α-Galactosylceramide-induced antimetastatic activity and Th 1 and Th 2 responses of NKT cells2006
Author(s)
Okajo, J., Kaneko, Y., Murata, Y., Okazawa, H., Ohnishi, H., Nojima, Y., and Matozaki, T.
Organizer
The 65th annual meeting of the Japanese Cancer Association
Place of Presentation
Yokohama
Year and Date
2006-09-28
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Presentation] Regulation by CD47 of epithelial cell spreading and migration and its signal transduction2006
Author(s)
Shinohara, M., Ohyama, N., Murata, Y., Okazawa, H., Ohnishi, H., and Matozaki, T.
Organizer
The 16th International Symposium on Regulatory Peptides (REGPEP' 06)
Place of Presentation
Hakone
Year and Date
2006-08-30
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Presentation] CD47 promotes neuronal development through Src-and FRG/Vav2-mediated activation of Rac and Cdc422006
Author(s)
Murata, T., Ohnishi, H., Okazawa, H., Murata, Y., Kusakari, S., Hayashi, Y., Miyashita, M., Furuya, N., and Matozaki, T.
Organizer
20th IUBMB International Congress of Biochemistry and Molecular Biology and 11th FAOBMB Congress
Place of Presentation
Kyoto
Year and Date
2006-06-18
Description
「研究成果報告書概要(欧文)」より
Related Report
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