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Quantification of molecular dynamics, numbers and interactions of multi-kind molecules by single molecule imaging in cells

Research Project

Project/Area Number 18370065
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Biophysics
Research InstitutionNational Institute of Genetics

Principal Investigator

TOKUNAGA Makio  National Institute of Genetics, Structural Biology Center, Professor (00192659)

Co-Investigator(Kenkyū-buntansha) SAKATA Kumiko (SOGAWA, Kumiko)  Riken Research Center for Allergy, Research Unit for Single Molecule immunoimaging, Unit Leader (20291073)
Project Period (FY) 2006 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥18,080,000 (Direct Cost: ¥15,200,000、Indirect Cost: ¥2,880,000)
Fiscal Year 2007: ¥12,480,000 (Direct Cost: ¥9,600,000、Indirect Cost: ¥2,880,000)
Fiscal Year 2006: ¥5,600,000 (Direct Cost: ¥5,600,000)
Keywordssingle molecule imaging / molecular systems / signal transduction / three-dimensional imaging / multi-color imaging / HILO microscopy / molecular quantification / immune cells / 免疫細胞活性化 / 転写因子 / 薄層斜光証明法 / 蛍光顕微鏡
Research Abstract

We have developed technologies that allow cellular functions such as immunological responses and signaling processes are to be visualized at the single-molecule level. Single molecule imaging coupled with the ability to simultaneously visualize several different proteins in cells has enabled the quantification of molecular dynamics, interactions, and kinetics. These three-dimensional and temporal parameters enables us to carry out numerical modeling and computer simulations of cell functions, to open up new frontiers for cellular functions as molecular systems.
We have demonstrated that clear visualization of single molecules in cells enablesd their molecular quantification. Clear single-molecule visualization was achieved using TIRF and HILO microscopy. The main technical challenge of single-molecule fluorescence imaging is increasing the signal/background ratio. We have been involved in the development of total internal reflection fluorescence (TIRF) microscopy, a light-microscopic te … More chnique that uses evanescent light to illuminate single molecules. TIRF has become a widespread technique for single-molecule imaging at surfaces, but cannot be used for single-cell imaging due to the very limited depth of evanescent light. We have achieved notable success in overcoming this limitation by inclining the illumination beam and by minimizing the illumination area.
Highly inclined and laminated optical sheet (HILO) microscopy is built upon the TIRF approach. Illumination by a highly inclined and thin beam increases image intensity and decreases background intensity, yielding a signal/background ratio about eightfold greater than that of epi-illumination. Most importantly, HILO can be used to visualize single molecules not only on cell surfaces but also inside living cells.
Single molecule microscopy using HILO and TIRF can be used for observations within living cells that are sensitive to the illuminated light, and for time-lapse observation over long periods since only weak illumination is required. Using HILO, the dynamic movements of large numbers of single molecules inside a cell can be traced. Thus, HILO is also useful for quantitative studies of the distribution, dynamic movement, or interaction of large numbers of molecules. We have installed a new multi-color system to observe intermolecular interactions in ever greater detail.
A high S/N ratio of images by HILO microscopy also yields clear three-dimensional images. We reconstructed a three-dimensional image from serial images of nuclear pore complexes. It demonstrated that HILO microscopy enables researchers to visualize three-dimensional molecular images which cannot be reconstructed by conventional confocal microscopy because of photo-bleaching of fluorescent dyes during three-dimensional scanning. Less

Report

(3 results)
  • 2007 Annual Research Report   Final Research Report Summary
  • 2006 Annual Research Report
  • Research Products

    (65 results)

All 2008 2007 2006

All Journal Article (24 results) (of which Peer Reviewed: 6 results) Presentation (39 results) Book (2 results)

  • [Journal Article] Highly inclined thin illumination enables clear single-molecule imaging in cells2008

    • Author(s)
      Tokunaga M.
    • Journal Title

      Nat.Methods 5

      Pages: 159-161

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] 細胞蛍光1分子イメージング2008

    • Author(s)
      十川 久美子
    • Journal Title

      遺伝子医学MOOK 9

      Pages: 192-196

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] 従来の8倍の高画質で生体分子1個を見る顕微鏡法を開発2008

    • Author(s)
      徳永 万喜洋
    • Journal Title

      メディカルバイオ 5

      Pages: 16-17

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Highly inclined thin illumination enables clear single-molecule imaging in cells.2008

    • Author(s)
      Tokunaga M., Imamoto N., Sakata-Sogawa, K.
    • Journal Title

      Nature Method. 5(2)

      Pages: 159-161

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Highly inclined thin illumination enables clear single-molecule imaging in cells.2008

    • Author(s)
      Tokunaga M.
    • Journal Title

      Nat. Methods 5

      Pages: 159-161

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 細胞蛍光1分子イメージング2008

    • Author(s)
      十川久美子
    • Journal Title

      遺伝子医学MOOK 9

      Pages: 192-196

    • Related Report
      2007 Annual Research Report
  • [Journal Article] 従来の8倍の高画質で生体分子1個を見る顕微鏡法を開発2008

    • Author(s)
      徳永万喜洋
    • Journal Title

      メディカルバイオ 5

      Pages: 16-17

    • Related Report
      2007 Annual Research Report
  • [Journal Article] Zinc is a novel second messenger2007

    • Author(s)
      Yamasaki S.
    • Journal Title

      J.Cell Biol. 177

      Pages: 637-645

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] 細胞1分子イメージングと定量解析〜シグナル伝達の可視化2007

    • Author(s)
      徳永 万喜洋
    • Journal Title

      バイオテクノロジージャーナル 7

      Pages: 659-663

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] 従来の8倍の高画質で生体分子1個を見る顕微鏡法を開発2007

    • Author(s)
      十川 久美子
    • Journal Title

      日本臨牀 65

      Pages: 242-246

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Zinc is a novel second messenger.2007

    • Author(s)
      Yamasaki S, Sakata-Sogawa K, Hasegawa A, Suzuki T, Kabu K, Sato E, Kurosaki T, Yamashita S, Tokunaga M, Nishida K, Hirano T.
    • Journal Title

      JCellBiol. 177(4)

      Pages: 637-645

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Vavi Acidic Region Pyrosine 174 Is Required for the Formation of T Cell Receptor-induced Microclusters and Is Essential in T Cell Development and Activation.2007

    • Author(s)
      Miletic AV, Sakata-Sogawa K, Hiroshima M, Hamann MJ, Gomez TS, Ota N, Kloeppel T, Kanagawa O, Tokunaga M, Billadeau DD, Swat W.
    • Journal Title

      JBiol Chem. 281(50)

      Pages: 38257-38265

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Zinc is a novel second messenger.2007

    • Author(s)
      Yamasaki S.
    • Journal Title

      J. Cell Biol. 177

      Pages: 637-645

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 細胞1分子イメージングと定量解析〜シグナル伝達の可視化2007

    • Author(s)
      徳永万喜洋
    • Journal Title

      バイオテクノロジージャーナル 7

      Pages: 659-663

    • Related Report
      2007 Annual Research Report
  • [Journal Article] 免疫分子動態の可視化システム2007

    • Author(s)
      十川久美子
    • Journal Title

      日本臨牀 65.2

      Pages: 242-246

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Vav1 acidic region Tyrosine 174 is required for the formation of T cell receptor-induced microclusters and is essential in T cell development and activation2006

    • Author(s)
      Miletic A.V.
    • Journal Title

      J.Biol.Chem. 281

      Pages: 38257-38265

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Mechanistic basis of pre-T cell receptor-mediated autonomous signaling critical for thymocyte development2006

    • Author(s)
      Yamasaki S.
    • Journal Title

      Nature Immunol. 7

      Pages: 67-75

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] 神経シナプス可塑性における局所的翻訳の制御機構2006

    • Author(s)
      椎名 伸之
    • Journal Title

      蛋白質・核酸・酵素 51

      Pages: 943-949

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] 必ず出来る2006

    • Author(s)
      徳永 万喜洋
    • Journal Title

      生物物理巻頭言 46

      Pages: 241-241

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Mechanistic basis of pre-T cell receptor-mediated autonomous signaling critical for thymocyte development.2006

    • Author(s)
      Yamaaki S., Ishikawa, E., Sakuma, M., Ogata, K., Sakata-Sogawa K., Hiroshima, M., Wiest, D. L., Tokunaga M., Saito, T.
    • Journal Title

      Nature Immunol 7(1)

      Pages: 67-75

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Vavl acidic region Tyrosine 174 is required for the formation of T cell receptor-induced microclusters and is essential in T cell development and activation2006

    • Author(s)
      A.V.Miletic
    • Journal Title

      J. Biol. Chem 281・50

      Pages: 38257-38265

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Mechanistic basis of pre-T cell receptor-mediated autonomous signaling critical for thymocyte development.2006

    • Author(s)
      Yamasaki S
    • Journal Title

      Nature Immunol 7.1

      Pages: 67-75

    • Related Report
      2006 Annual Research Report
  • [Journal Article] 神経シナプス可塑性における局所的翻訳の制御機構2006

    • Author(s)
      椎名伸之
    • Journal Title

      蛋白質・核酸・酵素 51・8

      Pages: 943-949

    • Related Report
      2006 Annual Research Report
  • [Journal Article] 「必ず出来る」2006

    • Author(s)
      徳永万喜洋
    • Journal Title

      生物物理巻頭言 46・5

      Pages: 241-241

    • Related Report
      2006 Annual Research Report
  • [Presentation] Zinc is a novel intracellular second messenger2008

    • Author(s)
      Yamasaki S.
    • Organizer
      1st meeting of the Society for Zinc Biology
    • Place of Presentation
      Banff,Canada
    • Year and Date
      2008-02-02
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
  • [Presentation] Zinc is a novel intracellular second messenger2008

    • Author(s)
      Yamasaki S, Sakata-Sogawa K, Hasegawa A, Suzuki T, Kabu K, Sato E, Kurosaki T, Yamashita S, Tokunaga M, Nishida K, Hirano T.
    • Organizer
      1st meeting of the Society for Zinc Biology, Banff
    • Place of Presentation
      Canada
    • Year and Date
      2008-02-02
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] DNA2重らせん塩基対の水素結合1個の力が計測される理由:分子動力学シミュレーションとの比較2007

    • Author(s)
      徳永 万喜洋
    • Organizer
      日本生物物理学会第45回年会
    • Place of Presentation
      横浜
    • Year and Date
      2007-12-23
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
  • [Presentation] 1分子イメージングによる転写因子の核内動態解析2007

    • Author(s)
      十川 久美子
    • Organizer
      日本生物物理学会第45回年会
    • Place of Presentation
      横浜
    • Year and Date
      2007-12-23
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
  • [Presentation] 転写因子の免疫刺激による共局在の変化:マルチカラー分子イメニジング2007

    • Author(s)
      新倉 和美
    • Organizer
      日本生物物理学会第45回年会
    • Place of Presentation
      横浜
    • Year and Date
      2007-12-23
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 転写因子の免疫刺激による共局在の変化:マルチカラー分子イメージング2007

    • Author(s)
      新倉和美
    • Organizer
      日本生物物理学会第45回年会
    • Place of Presentation
      横浜
    • Year and Date
      2007-12-23
    • Related Report
      2007 Annual Research Report
  • [Presentation] 神経RNA granuleタンパク質RNG105ノックアウトによる興奮性,抑制性シナプスバランスの異常2007

    • Author(s)
      椎名 伸之
    • Organizer
      日本生物物理学会第45回年会
    • Place of Presentation
      横浜
    • Year and Date
      2007-12-21
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 1分子計測およびMDシミュレーションによるタンパク質アンフォールディングの確率的経路と複数の中間状態の検出2007

    • Author(s)
      深川 暁宏
    • Organizer
      日本生物物理学会第45回年会
    • Place of Presentation
      横浜
    • Year and Date
      2007-12-21
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
  • [Presentation] 神経RNA granuleタンパク質RNG105ノックアウトによる興奮性・抑制性シナプスバランスの異常2007

    • Author(s)
      椎名伸之
    • Organizer
      日本生物物理学会第45回年会
    • Place of Presentation
      横浜
    • Year and Date
      2007-12-21
    • Related Report
      2007 Annual Research Report
  • [Presentation] 神経RNA granuleタンパク質RNG105による局所的翻訳制御とシナプス形成2007

    • Author(s)
      椎名 伸之
    • Organizer
      第30回日本分子生物学会年会・第80回日本生化学会大会 合同大会(BMB2007)
    • Place of Presentation
      横浜
    • Year and Date
      2007-12-15
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
  • [Presentation] 細胞内分子ダイナミクス・相互作用の1分子イメージングと3次元・マルチカラー・定量2007

    • Author(s)
      徳永 万喜洋
    • Organizer
      第30回日本分子生物学会年会・第80回日本生化学会大会 合同大会(BMB2007)
    • Place of Presentation
      横浜
    • Year and Date
      2007-12-13
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
  • [Presentation] TCR microcluster formation-Vav1 acidic region Tyr174 is essential for TCR signaling2007

    • Author(s)
      Sakata-Sogawa K
    • Organizer
      International Symposium on Membrane Traffic
    • Place of Presentation
      淡路
    • Year and Date
      2007-11-27
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] TCR microcluster formation -Vav1 acidic region Tyr174 is essential for TCR signaling2007

    • Author(s)
      Sakata-Sogawa K
    • Organizer
      International Symposium on Membrane Traffic
    • Place of Presentation
      淡路
    • Year and Date
      2007-11-27
    • Related Report
      2007 Annual Research Report
  • [Presentation] Mufti-color molecular imaging of transcription factors in T cell activation2007

    • Author(s)
      Sakata-Sogawa K
    • Organizer
      Focus on Microscopy 2007
    • Place of Presentation
      Valencia,スペイン
    • Year and Date
      2007-04-11
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Multi-color molecular imaging of transcription factors in T cell activation2007

    • Author(s)
      Sakata-Sogawa K
    • Organizer
      Focus on Microscopy 2007
    • Place of Presentation
      Valencia,スペイン
    • Year and Date
      2007-04-11
    • Related Report
      2007 Annual Research Report
  • [Presentation] Spatiotemporal regulation of T cell activation by TCR-CD28-microclusters2007

    • Author(s)
      Yokosuka T
    • Organizer
      13th International Congress of Immunology
    • Place of Presentation
      Rio de Janeiro,Brazil
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] A deficiency of the RNA granule protein RNG105 impairs the excitatory/inhibitory synaptic balance2007

    • Author(s)
      Shiina, N
    • Organizer
      第59回日本細胞生物学会大会・第40回日本発生生物学会年会・合同大会
    • Place of Presentation
      福岡
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
  • [Presentation] Evidence for hyper-dynamics of Polycomb repressive complexes by quantitative imaging and high-speed imaging2007

    • Author(s)
      Isono, K
    • Organizer
      第59回日本細胞生物学会大会・第40回日本発生生物学会年会・合同大会
    • Place of Presentation
      福岡
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
  • [Presentation] Multi-color molecular imaging of transcription factors in T cell activation2007

    • Author(s)
      Sakata-Sogawa K., Shinkura K., Kimura H., Tokunaga M.
    • Organizer
      Focus on Microscopy
    • Place of Presentation
      Valencia, Spain
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Spatiotemporal regulation of T cell activation by TCR-CD28-microclusters2007

    • Author(s)
      Yokosuka T., Sakata-Sogawa K., Kobayashi W., Hiroshima M., Hashimoto-Tane A., Tokunaga M., Dustin M. L., Saito T.
    • Organizer
      13th International Congress of Immunology Rio de Janeiro
    • Place of Presentation
      Brazil
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] TCR microcluster formation-Vavl acidic region Tyr174 is essential for TCR signaling2007

    • Author(s)
      Sakata-Sogawa K., Miletic A. V., Swat W., Tokunaga M.
    • Organizer
      International Symposium on Membrane Traffic, Awaji City
    • Place of Presentation
      Hyogo
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Kinetic Quantification and Modelhng of Nuclear Transport in Cells using Single Molecule Imaging2006

    • Author(s)
      Tokunaga M.
    • Organizer
      Gordon Research Conference Single Molecule Approaches To Biology
    • Place of Presentation
      New Hampshire,米国
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Molecular imaging and analysis of microclusters responsible for initiating T cell receptor signaling2006

    • Author(s)
      Sakata-Sogawa K.
    • Organizer
      Cordon Research Conference Single Molecule Approaches To Biology
    • Place of Presentation
      New Hampshire,米国
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Single hydrogen bonds of DNA base pairs detected by intermolecular force microscopy2006

    • Author(s)
      Hiroshima M.
    • Organizer
      Gordon Research Conference Single Molecule Approaches To Biology
    • Place of Presentation
      New Hampshire,米国
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] An RNA-binding protein RNG105 in neuronal RNA granules:regulatory machinery for local translation and synaptic plasticity2006

    • Author(s)
      Shiina N.
    • Organizer
      20th IUBMB International Congress of Biochemis try and Molecular Biology and 11th FAOBMB Congress
    • Place of Presentation
      京都
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Molecular imaging and analysis of microclusters responsible for initiating T cell receptor signaling2006

    • Author(s)
      Sakata-Sogawa K.
    • Organizer
      Fifth East Asian Biophysics Symposium & Forty-Fourth Annual Meeting of the Biophysical Society of Japan
    • Place of Presentation
      宜野湾,沖縄
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] RNG105 in neuronal RNA granules:Involvement in local translation and synapse formation2006

    • Author(s)
      Shiina N.
    • Organizer
      Fifth East Asian Biophysics Symposium & Forty-Fourth Annual Meeting of the Biophysical Society of Japan
    • Place of Presentation
      宜野湾,沖縄
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Multi-color molecular imaging of transcription factors2006

    • Author(s)
      Shinkura K.
    • Organizer
      Fifth East Asian Biophysics Symposium & Forty-Fourth Annual Meeting of the Biophysical Society of Japan
    • Place of Presentation
      宜野湾,沖縄
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Single hydrogen bonds of DNA base pairs detected by intermolecular force microscopy2006

    • Author(s)
      Hiroshima M.
    • Organizer
      Fifth East Asian Biophysics Symposium & Forty-Fourth Annual Meeting of the Biophysical Society of Japan
    • Place of Presentation
      宜野湾,沖縄
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Detection of substructural unfolding of SNase by intermolecular force microscopy2006

    • Author(s)
      Fukagawa A.
    • Organizer
      Fifth East Asian Biophysics Symposium & Forty-Fourth Annual Meeting of the Biophysical Society of Japan
    • Place of Presentation
      宜野湾,沖縄
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Kinetic Quantification and Modelling of Nuclear Transport in Cells using Single Molecule Imaging2006

    • Author(s)
      Tokunaga M. Imamoto N.
    • Organizer
      Gordon Research Conference Single Molecule Approaches 7b Biology
    • Place of Presentation
      New Hampshire, U.S.A.
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Molecular imaging and analysis of microclusters responsible for initiating T cell receptor signaling2006

    • Author(s)
      Sakata-Sogawa K., Yokosuka T., Michio H., Saito T., Tokunaga M.
    • Organizer
      Gordon Research Conference Single Molecule Approaches To Biology
    • Place of Presentation
      New Hampshire, U.S.A.
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Single hydrogen bonds of DNA base pairs detected by intermolecular force microscopy2006

    • Author(s)
      Hiroshima M., Tokunaga M.
    • Organizer
      Gordon Research Conference Single Molecule Approaches To Biology
    • Place of Presentation
      New Hampshire, U.S.A.
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] An RNA-binding protein RNG105 in neuronal RNA granules : regulatory machinery for local translation and synaptic plasticity2006

    • Author(s)
      Shiina N., Shinkura K., Tokunaga M.
    • Organizer
      20th IUBMB International Congress of Biochemistry and Molecular Biology and 11th FAOBMB Congress
    • Place of Presentation
      Kyoto
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Molecular imaging and analysis of microclusters responsible for initiating T cell receptor signaling2006

    • Author(s)
      Sakata-Sogawa K., Yokosuka T., Michio H., Saito T., Tokunaga M.
    • Organizer
      Fifth East Asian Biophysics Symposium, Forty-Fourth Annual Meeting of the Biophysical Society
    • Place of Presentation
      Japan, Ginowan City Okinawa
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Tokunaga M RNG105 in neuronal RNA granules : Involvement in local translation and synapse formation2006

    • Author(s)
      Shiina N., Shinkura K.
    • Organizer
      Fifth East Asian Biophysics Symposium, Forty-Fourth Annual Meeting of the Biophysical Society
    • Place of Presentation
      Japan, Ginowan City Okinawa
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Multi-color molecular imaging of transcription factors2006

    • Author(s)
      Shinkura K., Sakata-Sogawa K., Hiroshima M., Tokunaga M.
    • Organizer
      Fifth East Asian Biophysics Symposium, Forty-Fourth Annual Meeting of the Biophysical Society
    • Place of Presentation
      Japan, Ginowan City Okinawa
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Single hydrogen bonds of DNA base pairs detected by intermolecular force microscopy2006

    • Author(s)
      Hiroshima M., Tokunaga M.
    • Organizer
      Fifth East Asian Biophysics Symposium, Forty-Fourth Annual Meeting of the Biophysical Society
    • Place of Presentation
      Japan, Ginowan City Okinawa
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Detection of substructural unfolding of SNase by intermolecular force microscopy2006

    • Author(s)
      Fukagawa A., Hiroshima M., Kuwajima K., Tokunaga M.
    • Organizer
      Fifth East Asian Biophysics Symposium, Forty-Fourth Annual Meeting of the Biophysical Society
    • Place of Presentation
      Japan, Ginowan City Okinawa
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Book] アクチン・ミオシン分子モーター系, "生物物理ハンドブック"2007

    • Author(s)
      徳永 万喜洋
    • Publisher
      朝倉書店
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Book] アクチン・ミオシン分子モーター系, "生物物理ハンドブック"2007

    • Author(s)
      徳永万喜洋
    • Publisher
      朝倉書店
    • Related Report
      2007 Annual Research Report

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Published: 2006-04-01   Modified: 2016-04-21  

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