Development of real-time analysis system of molecular interactions using molecular imaging technique

• Project/Area Number: 18370066
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Biophysics
• Research Institution: Kyoto University (2008); Tokyo Metropolitan Organization for Medical Research (2006-2007)
• Principal Investigator: HARADA Yoshie Kyoto University, 物質-細胞統合システム拠点, 教授 (10202269)
• Co-Investigator(Kenkyū-buntansha): 横田 浩章 京都大学, 物質-細胞統合システム拠点, 特定拠点講師 (90415547)
• Project Period (FY): 2006 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥18,080,000 (Direct Cost: ¥15,200,000、Indirect Cost: ¥2,880,000); Fiscal Year 2008: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000); Fiscal Year 2007: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000); Fiscal Year 2006: ¥5,600,000 (Direct Cost: ¥5,600,000)
• Keywords: マイクロアレイ / 全反射顕微鏡 / 生体分子 / マイクロビーズ

Research Abstract

生体分子を結合させたマイクロビーズをガラス基板上にアレイ化する技術と全反射蛍光顕微鏡を組み合わせ、生体分子間相互作用を高感度に解析できる顕微鏡システムの開発を行った。このシステムは、微量な試料で複数の生体分子間の結合・解離過程を同時に実時間解析することができる。このシステムを使って、抗原-抗体反応、タンパク質-DNA 間相互作用、低分子化合物-タンパク質間の相互作用解析だけでなく、抗体を利用し、生物試料抽出液中の特定タンパク質の濃度を結合速度から定量できることを示した。

Research Products

• [Journal Article] Single-molecule visualization of binding modes of helicase to DNA on PEGylated surface (2009)
  • Author(s): H.Yokota, Y.-W. Han, J.-F. Allemand, X.-G. Xi, D. Bensimon,V. Croquette,Y. Ito, and Y. Harada
  • Journal Title: Chemistry Letters 38 Pages: 308-309
  • NAID: 10025120568
• [Journal Article] Hydrophilic fluorescent nanogel thermometer for intracellular thermometry. (2009)
  • Author(s): C. Gota, K. Okabe, T. Funatsu, Y. Harada, S. Uchiyama
  • Journal Title: Journal of the American Chemical Society 131 Pages: 2766-2767
  • Peer Reviewed
• [Journal Article] Single-molccule visualization of binding modes of helicase to DNA on PEGylated surface. (2009)
  • Author(s): H. Yokota, Y. -W. Man, J. -F. Allemand, X, -G. Xi, D, Bensimon, V. Croquctte, Y. Ito, Y. Harada
  • Journal Title: Chemistry Letters 38 Pages: 308-309
  • Peer Reviewed
• [Journal Article] Single-cell chemical lysis method for analyses of intracellular molecules using an array of picoliter-scale microwells Anal (2008)
  • Author(s): Y. Sasuga, T. Iwasawa, K. Terada, Y. Oe, H. Sorimachi, O. Ohara, and Y. Harada
  • Journal Title: Chem 80 Pages: 9141-9149
• [Journal Article] Single-cell chemical lysis method for analyses of intracellular molecules using an array of picoliter-scalc microwells (2008)
  • Author(s): Y. Sasuga, T. Iwasawa, K. Terada, Y. Oe, H. Sorimachi, O. Ohara, Y. Harada
  • Journal Title: Analytical Chemistry 80 Pages: 9141-9149
  • Peer Reviewed
• [Journal Article] Direct observation of the reversible unwinding of a single DNA molecule caused by the intercalation of ethidium bromide (2007)
  • Author(s): Masahito Hayashi, and Yoshie Harada
  • Journal Title: Nucleic Acids Research 35(19)
• [Journal Article] Dynamic polymorphism of actin as activation mechanism for cell motility. (2007)
  • Author(s): Kozuka, J.
  • Journal Title: Biosystems 88(3) Pages: 273-282
  • Peer Reviewed
• [Journal Article] Studies of DNA-Protein Interactions at the Single Molecule Level with Magnetic Tweezers. (2007)
  • Author(s): Allemand, J., -F.
  • Journal Title: Lect.Notes Phys. 711 Pages: 123-140
• [Journal Article] Direct observation of the reversible unwinding of a single DNA molecule caused by the intercalation of ethidium bromide. (2007)
  • Author(s): Hayashi, M.
  • Journal Title: Nucleic Acids Res. 35(19)
  • Peer Reviewed
• [Journal Article] Visualization of RecA filaments and DNA by fluorescence microscopy. (2007)
  • Author(s): Nishinaka, T.
  • Journal Title: J. Biochem. 141・2 Pages: 147-156
• [Journal Article] Direct observation of DNA rotation during branch migration of Holliday junction DNA by Escherichia coli RuvA-RuvB protein complex (2006)
  • Author(s): Yong-Woon Han, Tomomi Tani, Masahito Hayashi, Takashi Hishida, Hiroshi Iwasaki, Hideo Shinagawa and Yoshie Harada
  • Journal Title: Proc. Natl. Acad Sci. USA 103(31) Pages: 11544-11548
• [Journal Article] Development of a microscopic platform for real-time monitoring of biomolecular interactions (2006)
  • Author(s): Yasuhiro Sasuga, Tomomi Tani, Masahito Hayashi, Hisashi Yamakawa, Osamu Ohara, and Yoshie Harada
  • Journal Title: Genome Res 16 Pages: 132-139
• [Journal Article] Dynamic polymorphism of Ras observed by single molecule FRET is the basis for molecular recognition. (2006)
  • Author(s): Arai Y.
  • Journal Title: Biochem. Biophys. Res. Commun. 343・3 Pages: 809-815
• [Journal Article] 1分子蛍光測定におけるブリンキングの問題 (2006)
  • Author(s): 横田浩章
  • Journal Title: 生物物理 46・3 Pages: 164-168
  • NAID: 110004750173
• [Journal Article] Direct observation of DNA rotation during branch migration of Holliday junction DNA Escherichia coli RuvA-RuvB protein complex. (2006)
  • Author(s): Han, Y-W.
  • Journal Title: Proc. Natl. Acad. Sci. USA 103・31 Pages: 11544-11548
• [Journal Article] バイオマシン・生物分子モーター (2006)
  • Author(s): 原田慶恵
  • Journal Title: 細胞工学 25・8 Pages: 878-883
• [Presentation] Direct observation of DNA rotation during branch migration of Holliday junction DNA by Escherichia coli RuvA-RuvB protein complex (2009)
  • Author(s): Yong-Woon Han, Tomomi Tani, Masahito Hayashi, Takashi Hishida, Hiroshi Iwasaki, Hideo Shinagawa, and Yoshie Harada
  • Organizer: International symposium on Bio-nanosystems
  • Place of Presentation: 仙台
• [Presentation] A simple single-cell lysis method for analyses of intracellular molecules using a picoliter-scale microwell array (2009)
  • Author(s): Yasuhiro Sasuga, Yoshihiro Ooe, Osamu Ohara, Yoshie Harada
  • Organizer: The 6^<th> Asian Biophysics Association (ABA) Synposium 2009
  • Place of Presentation: Hong Kong
• [Presentation] A novel protein array using microbeads aligned in a microfluidic chip (2009)
  • Author(s): Yoshihiro Ooe, Yasuhiro Sasuga Osamu Ohara and Yoshie Harada
  • Organizer: Biophysical Society 53^<rd> Annual Meeting
  • Place of Presentation: Boston USA
• [Presentation] A simple single-cell lysis method for analyses of intracellular molecules using a picoliter-scale mierowell array (2009)
  • Author(s): Yasuhiro Sasuga, Yoshihiro Ooc, Osamu Ohara, Yoshie Harada
  • Organizer: The 6th Asian Biophyses Association (ABA) Synposium
  • Place of Presentation: Hong Kong
• [Presentation] A novel protein array using microbeads aligned in a microfluidie chip (2009)
  • Author(s): Yoshiro Ooc, Yasuhiro Sasuga Osamu Ohara, Yoshic Harada
  • Organizer: Biophysical Society 53rd Annual Meeting
  • Place of Presentation: Boston USA
• [Presentation] Studies on biomolecules using single-molecule imaging and manipulation techniques (2008)
  • Author(s): Yoshie Harada
  • Organizer: 8^<th> International Conference on Excitonic Processes in Condensed Matter (EXCON'08)
  • Place of Presentation: Kyoto (Invited)
  • Year and Date: 2008-06-26
• [Presentation] マイクロ流路とマイクロビーズを組み合わせた新規生体分子間相互作用検出法 (2008)
  • Author(s): 大江良洋、貴家康尋、原田慶恵
  • Organizer: 第8 回日本蛋白質科学会年会
  • Place of Presentation: 東京
  • Year and Date: 2008-06-11
• [Presentation] マイクロ流路とマイクロビーズを組み合わせた新規生体分子間相互作用検出法 (2008)
  • Author(s): 大江良洋, 貴家康尋, 原田慶恵
  • Organizer: 第8回日本蛋白質科学会年会
  • Place of Presentation: タワーホール船堀 東京
  • Year and Date: 2008-06-11
• [Presentation] マイクロビーズアレイとマイクロウェルを使った1細胞タンパク質解析法の開発 (2008)
  • Author(s): 原田慶恵
  • Organizer: バイオナノ研究会
  • Place of Presentation: 宮城 青根温泉
  • Year and Date: 2008-05-09
• [Presentation] Single-molecule experiments on DNA motors The First iCeMS Symposium and the 11th Membrane Research Forum (2008)
  • Author(s): Yoshie Harada
  • Place of Presentation: Kyoto
  • Year and Date: 2008-02-21
• [Presentation] Direct visualization of single-biomolecule dynamics using novel microscopy. (2008)
  • Author(s): Yokota, H.
  • Organizer: iCeMS Job Seminar
  • Place of Presentation: Kyoto City
  • Year and Date: 2008-01-28
• [Presentation] DNAモーターが引き起こすDNAねじれ回転運動の直接観察. (2008)
  • Author(s): 原田 慶恵
  • Organizer: ナノバーチャル合同シンポジウム
  • Place of Presentation: 東京
  • Year and Date: 2008-01-22
• [Presentation] Single-molecule Observation of DNA/helicase Interaction by Novel Microscopy (2008)
  • Author(s): Yokota, H., Han, Y-W., Allemand, J-F., Xie, X., Croquette, V., Bensimon, D., Harada, Y
  • Organizer: Joint Meeting of the Biophysical Society 52^<nd> Annual Meeting &amp; 16^<th> International Biophysics Congress
  • Place of Presentation: Long Beach
• [Presentation] Real Time Quantitation of an Endogenous mRNA in Single Living Cells (2008)
  • Author(s): Okabe, K., Harada, Y. and Funatsu, T
  • Organizer: Joint Meeting of the Biophysical Society 52^<nd> Annual Meeting &amp; 16^<th> International Biophysics Congress
  • Place of Presentation: Long Beach
• [Presentation] Effect of Zipcode Sequence and Molecular Weight of mRNA on Its Subcellular Localization Revealed by Single Molecule Imaging (2008)
  • Author(s): Yamagishi, M., Ishihama, Y., Shirasaki, Y., Kurama, H. Sasuga, Y., Terada, K., Harada, Y., Funatsu, T
  • Organizer: Joint Meeting of the Biophysical Society 52^<nd> Annual Meeting &amp; 16^<th> International Biophysics Congress
  • Place of Presentation: Long Beach
• [Presentation] Single-molecule Observation of DNA/helicase Interaction by Novel Microscopy.. The first iCeMS Symposium (2008)
  • Author(s): Yokota, H., Han, Y-W., Allemand, J-F., Xie, X., Croquette, V., Bensimon, D. and Harada, Y
  • Organizer: featuring mesoscopic interactions in cells and cellular membranes and the 11th International Membrane Research Forum
  • Place of Presentation: Kyoto City
• [Presentation] Harada A novel protein array using microbeads aligned in a microfluidic chip (2008)
  • Author(s): Yoshihiro Ooe, Ysuhiro Sasuga, Osamu Ohara and Yoshie Harada
  • Organizer: 日本生物物理学会 第46 回年会
  • Place of Presentation: 福岡
• [Presentation] A simple single-cell chemical lysis metyhod for analyses of intraccllular molecules using a picoliter-scalc microwell array (2008)
  • Author(s): Sasuga, Y., lwasawa, T., Ooc, Y., Ohara, O., Ilarada, Y
  • Organizer: Single molecule approaches to biology Gordon Research Conferences
  • Place of Presentation: Colby-Sawyer College USA
• [Presentation] A novel protein array using microbcads aligned in a niicrofluidic chip (2008)
  • Author(s): Yoshihiro Ooc, Ysuhiro Sasuga, Osamu Ohara, Yoshic Harada
  • Organizer: 日本生物物理学会第46回年会
  • Place of Presentation: 福岡国際会議場
• [Presentation] 転写バブル形成時のDNAの構造変化の可視化. (2008)
  • Author(s): 加藤 悠子
  • Organizer: 2008年生体運動研究回合同班会議
  • Place of Presentation: 仙台
• [Presentation] DNA/タンパク質間相互作用の高精度1分子多次元解析. (2008)
  • Author(s): 横田 浩章
  • Organizer: 第5回JSTさきがけ「生命現像と計測分析」領域会議
  • Place of Presentation: 大阪市
• [Presentation] Single-molecule Observation of DNA/helicase Interaction by Novel Microscopy. (2008)
  • Author(s): Yokota, H.
  • Organizer: Joint Meeting of the Biophysical Society 52^<nd> Annual Meeting & 16^<th> International Biophysics Congress
  • Place of Presentation: Long Beach.
• [Presentation] Real Time Quantitation of an Endogenous mRNA in Single Living Cells. (2008)
  • Author(s): Okabe, K.
  • Organizer: Joint Meeting of the Biophysical Society 52^<nd> Annual Meeting & 16^<th> International Biophysics Congress
  • Place of Presentation: Long Beach
• [Presentation] Effect of Zipcode Sequence and Molecular Weight of mRNA on Its Subcellular Localization Revealed by Single Molecule Imaging. (2008)
  • Author(s): Yamagishi, M.
  • Organizer: Joint Meeting of the Biophysical Society 52^<nd> Annual Meeting & 16^<th> International Biophysics Congress
  • Place of Presentation: Long Beach
• [Presentation] Single-molecule Observation of DNA/helicase Interaction by Novel Microscopy. (2008)
  • Author(s): Yokota, H.
  • Organizer: The first iCeMS Symposium, featuring mesoscopic interactions in cells and cellular membranes and the 11th International Membrane Research Forum
  • Place of Presentation: Kyoto City
• [Presentation] マイクロビーズを利用したin vitro生体分子間相互作用解析法と単一細胞解析技術の開発 (2007)
  • Author(s): 貴家康尋, 小原收, 原田慶恵
  • Organizer: BMB2007 ワークショップ
  • Place of Presentation: 横浜(招待講演)
  • Year and Date: 2007-12-11
• [Presentation] マイクロ流路を利用した迅速なタンパク質相互作用検出システムの開発 (2007)
  • Author(s): 薄井(青木)一恵, 貴家康尋, 岡崎規理子,小原玲子, 原田慶恵, 小原收
  • Organizer: BMB2007
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-11
• [Presentation] マイクロビーズを利用したin vitro生体分子間相互作用解析法と単一細胞解析技術の開発.(招待講演) (2007)
  • Author(s): 貴家 康尋
  • Organizer: BMB2007(第30回日本分子生物学会・第80回日本生化学会大会合同大会)ワークショップ
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-11
• [Presentation] 生細胞内でのmRNA動態解析による局在化機構の解明. (2007)
  • Author(s): 山岸 舞
  • Organizer: BMB2007(第30回日本分子生物学会・第80回日本生化学会大会合同大会)
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-11
• [Presentation] マイクロ流路を利用した迅速なタンパク質相互作用検出システムの開発. (2007)
  • Author(s): 薄井(青木)一恵
  • Organizer: BMB2007(第30回日本分子生物学会・第80回日本生化学会大会合同大会)
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-11
• [Presentation] ナノマシーンとバイオイメージング.,(招待講演) (2007)
  • Author(s): 原田 慶恵
  • Organizer: 第16回日本バイオイメージング学会学術集会
  • Place of Presentation: 野田(千葉県)
  • Year and Date: 2007-10-31
• [Presentation] -分子イメージングで生体分子の機能を探る. (2007)
  • Author(s): 原田 慶恵
  • Organizer: 分子・細胞・組織操作を目指したシステム細胞工学-女性研究者による理学医学工学融合分野の現状と動向-
  • Place of Presentation: 東京
  • Year and Date: 2007-08-11
• [Presentation] タンパク質分子ダイナミクスの1分子計測. (2007)
  • Author(s): 横田 浩章:
  • Organizer: 慶應義塾大学理工学部物理学科談話会
  • Place of Presentation: 横浜
  • Year and Date: 2007-05-23
• [Presentation] DNA/タンパク質間相互作用の高精度1分子多次元解析. (2007)
  • Author(s): 横田 浩章
  • Organizer: 第2回生命・計測分析合同研究会
  • Place of Presentation: 東京
  • Year and Date: 2007-04-20
• [Presentation] 一細胞中の酵素活性測定技術の開発 (2007)
  • Author(s): 岩澤朋之、貴家康尋、原田慶恵
  • Organizer: 2007 年生体運動研究研究合同班会議
  • Place of Presentation: 金沢
• [Presentation] マイクロウェルアレイを用いた単一細胞分泌タンパク質解析法の開発 (2007)
  • Author(s): 貴家康尋, 寺田佳代子, 大江良洋, 小原收, 原田慶恵
  • Organizer: 日本生物物理学会第45 回年会
  • Place of Presentation: 横浜
• [Presentation] 光学顕微鏡による生体分子・細胞生物学研究のこれまでと将来展望. (2007)
  • Author(s): 横田 浩章
  • Organizer: 第4回放射光学会若手ワークショップ
  • Place of Presentation: 佐用町(兵庫県佐用郡)
• [Presentation] MRI・蛍光同時計測による生体内分子・細胞イメージング法の開発. (2007)
  • Author(s): 横田 浩章
  • Organizer: 第4回「生命現象と計測分析」領域会議
  • Place of Presentation: 小樽
• [Presentation] 生きた単一細胞における内在性mRNAのリアルタイム定量. (2007)
  • Author(s): 岡部 弘基
  • Organizer: 日本生物物理学会第45回年会
  • Place of Presentation: 横浜
• [Presentation] 細胞質の分子篩効果によるmRNAの細胞質内局在化. (2007)
  • Author(s): 山岸 舞
  • Organizer: 日本生物物理学会第45回年会
  • Place of Presentation: 横浜
• [Presentation] DNA helicase UvrDの一分子観察. (2007)
  • Author(s): 中条 裕子
  • Organizer: 日本生物物理学会第45回年会
  • Place of Presentation: 横浜
• [Presentation] 同時計測顕微鏡によるDNA/ヘリカーゼ相互作用の1分子観察. (2007)
  • Author(s): 横田 浩章
  • Organizer: 日本生物物理学会第45回年会
  • Place of Presentation: 横浜
• [Presentation] 転写開始時の転写バブル形成の可視化. (2007)
  • Author(s): 加藤 悠子
  • Organizer: 日本生物物理学会第45回年会
  • Place of Presentation: 横浜
• [Presentation] 切断された神経軸索の再生における神経成長因子の作用. (2007)
  • Author(s): 野村 真未
  • Organizer: 日本生物物理学会第45回年会
  • Place of Presentation: 横浜
• [Presentation] マイクロウェルアレイを用いた単一細胞分泌タンパク質解析法の開発. (2007)
  • Author(s): 貴家 康尋
  • Organizer: 日本生物物理学会第45回年会
  • Place of Presentation: 横浜
• [Presentation] Development of a microscopic platform for real-time monitoring of biomolecular interactions (2006)
  • Author(s): Sasuga, Y., Tani, T., Hayashi, M., Yamakawa, H., Ohara, O. and Harada, Y
  • Organizer: Gordon Research Conference 2006 Single Molecule Approaches To Biology
  • Place of Presentation: New London, USA
• [Presentation] Labeling of iron-sulfur protein with a fluorescent dye to detect its conformation change in the cytochrome bc1complex (2006)
  • Author(s): Miki, T., Sakamoto, S., Yokota, H., and Harada, Y
  • Organizer: Fifth East Asian Biophysics Symposium &amp; Forty-Fourth Annual Meeting of the Biophysical Society of Japan
  • Place of Presentation: Okinawa, Japan
• [Presentation] Direct observation of T7 RNA polymerase rotation during transcription (2006)
  • Author(s): Kato, Y., Yokota, H., Hayashi, M., Terada, K., Sasuga, Y. and Harada, Y
  • Organizer: Fifth East Asian Biophysics Symposium &amp; Forty-Fourth Annual Meeting of the Biophysical Society of Japan
  • Place of Presentation: Ginowan City,Japan
• [Presentation] Direct observation of the twisting motion of a single DNA molecule caused by the intercalation of ethidium bromide (2006)
  • Author(s): Hayashi, M. and Harada, Y
  • Organizer: Fifth East Asian Biophysics Symposium & Forty-Fourth Annual Meeting of the Biophysical Society of Japan
  • Place of Presentation: Ginowan City, Japan
• [Presentation] Novel microscopy for simultaneous single molecule measurement of DNA/protein interaction (2006)
  • Author(s): Yokota, H., Han, Y-W., Allemand, J-F., Xi, X., Croquette V. , Bensimon, and D., Harada Y
  • Organizer: Fifth East Asian Biophysics Symposium &amp; Forty-Fourth Annual Meeting of the Biophysical Society of Japan
  • Place of Presentation: Ginowan City, Japan
• [Presentation] Live imaging of growth cone regeneration after axotomy of dorsal root ganglion neurons (2006)
  • Author(s): Nomura, M., Tani, T., and Harada, Y
  • Organizer: Fifth East Asian Biophysics Symposium &amp; Forty-Fourth Annual Meeting of the Biophysical Society of Japan
  • Place of Presentation: Ginowan City, Japan
• [Presentation] Real Time Imaging of Specific Messenger RNA in a Living Cell Using Artificial Nucleic Acids (2006)
  • Author(s): Okabe, K., Harada, Y. and Funatsu, T
  • Organizer: Fifth East Asian Biophysics Symposium &amp; Forty-Fourth Annual Meeting of the Biophysical Society of Japan
  • Place of Presentation: Ginowan City, Japan
• [Presentation] Development of a microscopic platform for single-cell analyses using picoliter microwell array (2006)
  • Author(s): Sasuga, Y., Iwasawa, T., Terada, K., Sorimachi, H., Ohara, O. and Harada, Y
  • Organizer: Fifth East Asian Biophysics Symposium &amp; Forty-Fourth Annual Meeting of the Biophysical Society of Japan
  • Place of Presentation: Ginowan City, Japan
• [Presentation] A simple single-cell chemical lysis method for analyses of intracellular molecules using a picoliter-scale microwell array Gordon Research Conference
  • Author(s): Sasuga, Y., Iwasawa, T., Ooe, Y., Ohara, O., Harada, Y
• [Book] バイオマシン・生物分子モーター (2008)
  • Author(s): 原田慶恵、横田浩章
  • Publisher: 細胞工学秀潤社(Vol.25 No.8)
• [Book] 1分子が見えた!.1枚の写真館 (2008)
  • Author(s): 原田慶恵
  • Total Pages: 425
  • Publisher: 細胞工学(27(5))
• [Book] ナノバイオ辞典, テクノシステム (2007)
  • Author(s): 原田慶恵
  • Publisher: 一分子観察, 蛍光顕微鏡, 蛍光色素, 光ピンセット
• [Book] 一分子蛍光法化学と生物 (2007)
  • Author(s): 原田慶恵
  • Publisher: 日本農芸化学会 会誌(vol.45 No.5)
• [Book] DNA モーター生物物理学ハンドブック (2007)
  • Author(s): 原田慶恵
  • Publisher: 朝倉書店
• [Book] エヌ・ティー・エス (2007)
  • Author(s): 原田慶恵
  • Publisher: DNA モーター非侵襲・可視化技術ハンドブック
• [Book] 光ピンセット.生命科学のための機器分析実験ハンドブック (実験医学別冊) (2007)
  • Author(s): 横田浩章, 原田慶恵
  • Publisher: 第3章顕微解析4.(羊土社)
• [Book] 1分子イメージング. 生命科学のための機器分析実験ハンドブック(実験医学別冊) (2007)
  • Author(s): 原田慶恵
  • Publisher: 第7章イメージング解析 3.(羊土社)
• [Book] DNAモーター.生物物理学ハンドブック6.5 (2007)
  • Author(s): 原田 慶恵
  • Publisher: 朝倉書店
• [Book] 一分子蛍光法.化学と生物45(5) (2007)
  • Author(s): 原田 慶恵
  • Publisher: 日本農芸化学会
• [Book] DNAモーター.非侵襲・可視化技術ハンドブック-ナノ・バイオ・医療から情報システムまで-第7章1分子可視化と操作第5節 (2007)
  • Author(s): 原田 慶恵
  • Publisher: エヌ・テイー・エス
• [Book] 光ピンセット〜生体分子の操作と力・変位計測〜.生命科学のための機器分析実験ハンドブック(実験医学別冊)第3章顕微解析4. (2007)
  • Author(s): 横田 浩章
  • Publisher: 羊土社
• [Book] ナノバイオ辞典 一分子観察,蛍光顕微鏡,蛍光色素,光ピンセット (2007)
  • Author(s): 原田慶恵
  • Total Pages: 10
  • Publisher: テクノシステム
• [Book] DNA,RNAポリメラーゼ. 生物物理学ハンドブック (2007)
  • Author(s): 原田慶恵
  • Publisher: 朝倉書店(印刷中)
• [Book] 光トラップ 分子細胞生物学辞典
  • Author(s): 原田慶恵
  • Publisher: 光トラップ 分子細胞生物学辞典
• [Patent(Industrial Property Rights)] マイクロアレイ流路形成体を利用したマイクロビーズアレイ用チップ、マイクロビーズアレイ及びこれらを用いた被検物質を検出する方法 (2007)
  • Inventor(s): 貴家 康尋 原田 慶恵 小原 收
  • Industrial Property Rights Holder: (財)かずさディー・エヌ・エー研究所(財)東京都医学研究機構
  • Industrial Property Number: 2007-195476
  • Filing Date: 2007-07-27
• [Patent(Industrial Property Rights)] マイクロ流路形成体を利用したマイクロビーズアレイ用チップ、マイクロビーズアレイ及びこれらを用いた被検物質を検出する方法