Search for physiological substrates and functional analysis of F-box protein β-TrCP in vivo

• Project/Area Number: 18370075
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Cell biology
• Research Institution: Tohoku University
• Principal Investigator: NAKAYAMA Keiko Tohoku University, Tohoku University, Graduate School of Medicine, Professor (60294972)
• Co-Investigator(Kenkyū-buntansha): ISHIDA Noriko Tohoku University, Graduate School of Medicine, Assistant Professor (10361073) ; HARA Kentaro Tohoku University, Graduate School of Medicine, COE Fellow (60400248) ; 中谷 雅明 東北大学, 大学院医学系研究科, 助手 (70422095)
• Project Period (FY): 2006 – 2007
• Project Status: Completed (Fiscal Year 2007)
• Budget Amount: ¥17,270,000 (Direct Cost: ¥15,500,000、Indirect Cost: ¥1,770,000); Fiscal Year 2007: ¥7,670,000 (Direct Cost: ¥5,900,000、Indirect Cost: ¥1,770,000); Fiscal Year 2006: ¥9,600,000 (Direct Cost: ¥9,600,000)
• Keywords: Ubiquitin / F-box protein / knockout mouse / β-TrCP2 / circadian rhythm / 血管リモデリング / in situ hybridization

Research Abstract

Our purpose of this project is elucidation of the regulation of protein expression on development. We have analyzed the function of F-box proteins β-TrCP2, and demonstrated its role on development and differentiation during embryogenesis.
β-TrCP1 is the ubiquitin ligase which ubiquitinates β-catenin and l_<κ>βα The gene-targeted mouse of β-TrCP1, which we have generated, still exhibited degradation of β-catenin and l_<κ>βα, suggesting existence of other ubiquitin ligases for β-catenin and l_<κ>βα.
The gene-targeted mouse of β-TrCP2, the homologue of β-TrCP1 is embryonic lethal at embryonic day9.5. This difference of phenotype between β-TrCP1 knockout mouse and that of β-TrCP2, indicate that each proteins have different biological functions significantly, even though the difference of biochemical functions have not been reported.
We analyzed four proteins picked up based on the expression during early and middle embryogenesis and the consensus sequences, which are recognized by β-TrCP2. However, we did not detected bindings between these proteins and β-TrCP2, and ubiquitination and degradation by β-TrCP2.
We have generated β-TrCP2 conditional knockout mouse.
B-TrCP1 knockout primary embryonic fibroblasts have shown the abnormal regulation of Period 2 protein expression, suggesting that maintenance of circadian rhythm requires protein degradation by β-TrCP1.

Research Products

• [Journal Article] Fbxw7 acts as a critical failsafe against premature loss of hematopoietic stem cells and development of T-ALL. (2008)
  • Author(s): Matsuoka, S., et. al.
  • Journal Title: Genes Dev. 22 Pages: 986-991
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Targeting the p27 E3 ligase SCFSkp2 results in p27-and Skp2-mediated cell cycle arrest, and activation of autophagy. (2008)
  • Author(s): Chen, Q., et. al.
  • Journal Title: Blood 111 Pages: 4690-4699
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Skp2 regulates the antiproliferative function of the tumor suppressorRASSF1A via ubiquitin-mediated degradation at the G(1)-S transition. (2008)
  • Author(s): Song, M. S., et. al.
  • Journal Title: Oncogene (In press)
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] SCFβTrCP1 activates and ubiquitylates TAp63γ. (2008)
  • Author(s): Gallegos, J.R., et. al.
  • Journal Title: J Biol Chem. 283 Pages: 66-75
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] T. Fbxw7 acts as a critical failsafe against premature loss of hematopoietic stem cells and development of T-ALL (2008)
  • Author(s): Matsuoka, S., Oike, Y., Onoyama, I., Iwama, A., Arai, F., Takubo, K., Mashimo, Y., Oguro, H., Nitta, E., Ito, K., Miyamoto, K., Yoshiwara, H., Hosokawa, K., Nakamura, Y., Gomei, Y., Iwasaki, H., Hayashi, Y., Matsuzaki, Y., Nakayama, K., Ikeda, Y., Hata, A., Chiba, S., Nakayama, K.I., Suda, T.
  • Journal Title: Genes Dev 22 Pages: 986-991
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Targeting the p27 E3 ligase SCFSkp2 results in p27- and Skp2-mediated cell cycle arrest, and activation of autophagy (2008)
  • Author(s): Chen, Q., Xie, W., Kuhn, D.J., Voorhees, P.M., Lopez-Girona, A., Mendy, D., Corral, L.G., Krenitsky, V.P., Xu, W., Moutouh-de Parseval, L., Webb, D.R., Mercurio, F., Nakayama, K.I., Nakayama, K., Orlowski, R.Z.
  • Journal Title: Blood 111 Pages: 4690-4699
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Skp2 regulates the antiproliferative function of the tumor suppressor RASSF1A via ubiquitin-mediated degradation at the G(1)-S transition (2008)
  • Author(s): Song, M.S., Song, S.J., Kim, S.J., Nakayama, K., Nakayama, K.I. and Lim, D.S.
  • Journal Title: Oncogene (in press)
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] SCFβTrCP1 activates and ubiquitylates TAp63γ (2008)
  • Author(s): Gallegos, J.R., Litersky, J., Lee, H., Sun, Y., Nakayama, K., Nakayama, K. and Lu, H.
  • Journal Title: J Biol Chem. 283 Pages: 66-75
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] SCF型ユビキチンリガーゼによる細胞周期制御と発癌 (2008)
  • Author(s): 中山敬一and中山啓子
  • Journal Title: 実験医学(増刊) 26 Pages: 46-54
• [Journal Article] Essential role of Skp2-mediated p27 degradation in growth and adaptive expansion of pancreatic beta cells. (2007)
  • Author(s): Zhong, L., et. al.
  • Journal Title: J. Clin. Invest. 117 Pages: 2869-2876
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Negative feedback loop in the bim-caspase-3 axis regulating apoptosis and activity of osteoclasts. (2007)
  • Author(s): Wakeyama, H.
  • Journal Title: J. Bone. Miner. Res. 22 Pages: 1631-1639
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Ablation of p27 enhance kainate-induced seizure and hippocampal degeneration. (2007)
  • Author(s): Ueyama, C., et. al.
  • Journal Title: Neuroreport 18 Pages: 1781-1785
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Noncanonical Wnt signaling through G protein-linked PKCδactivation promotes bone formation. (2007)
  • Author(s): Tu, X., et. al.
  • Journal Title: Dev. Cell 12 Pages: 113-127
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Renal damage in obstructive nephropathy is decreased in Skp2-deficient mice. (2007)
  • Author(s): Suzuki, S., et. al.
  • Journal Title: Am. J. Pafhol. 171 Pages: 473-483
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Cyclin D2 Translocates p27 out of the Nucleus and Promotes Its Degradation at the G0-G1 Transition. (2007)
  • Author(s): Susaki, E., et. al.
  • Journal Title: Mol. Cell. Biol. 27 Pages: 4626-4640
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Skp2 controls adipocyte proliferation during the development of obesity. (2007)
  • Author(s): Sakai, T., et. al.
  • Journal Title: J. Biol. Chem 282 Pages: 2038-2046
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Asbestos-induced peribronchiolar cell proliferation and cytokine production are attenuated in lungs of protein kinase C-delta knockout mice (2007)
  • Author(s): Shukla, A., et. al.
  • Journal Title: Am. J. Pathol. 170 Pages: 140-151
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Conditional inactivation of Fbxw7 impairs cell-cycle exit during T cell differentiation and results in lymphomatogenesis (2007)
  • Author(s): Onoyama, l., et. al.
  • Journal Title: J. Exp. Med. 204 Pages: 2875-2888
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Foxo3a is essential for maintenance of the hematopoietic stem cell pool. (2007)
  • Author(s): Miyamoto, K., et. al.
  • Journal Title: Cell Stem Cell 1 Pages: 101-112
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] nPKC{varepsilon}, a P2Y2-R downstream effector in regulated mucin secretion from airway goblet cells (2007)
  • Author(s): Ehre, C., et. al.
  • Journal Title: Am. J. Physiol.-Cell Physiol. 293 Pages: 1445-1454
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] The F Box Protein S Phase Kinase-associated Protein 2 Regulates Adipose Mass and Adipocyte Number in Vivo. (2007)
  • Author(s): Cooke, P. S., et. al.
  • Journal Title: Obesity 15 Pages: 1400-1408
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Protein kinase C-delta phosphorylates Ebpl and prevents its proteolytic degradation, enhancing cell survival. (2007)
  • Author(s): Liu Z., et. al.
  • Journal Title: J. Neurochem. 100 Pages: 1278-1288
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] The cyclin-dependent kinase inhibitors p57 and p27 regulate neuronal migration in the developing mouse neocortex. (2007)
  • Author(s): Itoh, Y., et. al.
  • Journal Title: J. Biol. Chem. 282 Pages: 390-396
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Essential role of Skp2-mediated p27 degradation in growth and adaptive expansion of pancreatic beta cells (2007)
  • Author(s): Zhong, L., Georgia, S., Tschen, S.I., Nakayama, K., Nakayama, K.I., Bhushan, A.
  • Journal Title: J. Clin. Invest 117 Pages: 2869-2876
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Negative feedback loop in the bim-caspase-3 axis regulating apoptosis and activity of osteoclasts (2007)
  • Author(s): Wakeyama, H., Akiyama, T., Takahashi, K., Amano, H., Kadono, Y., Nakamura, M., Oshima, Y., Itabe, H., Nakayama, K.I., Nakayama, K., Nakamura, K. and Tanaka, S.
  • Journal Title: J. Bone. Miner. Res. 22 Pages: 1631-1639
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Ablation of p27 enhance kainate-induced seizure and hippocampal degeneration (2007)
  • Author(s): Ueyama, C., Akashiba, H., Nakayama, K., Nakayama, K.I., Nishiyama, N., Matsuki, N.
  • Journal Title: Neuroreport 18 Pages: 1781-1785
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Noncanonical Wnt signaling through G protein-linked PKCδactivation promotes bone formation (2007)
  • Author(s): Tu, X., Joeng, K.S., Nakayama, K.I., Nakayama, K., Rajagopal, J., Carroll, T.J., McMahon, A.P., Long, F.
  • Journal Title: Dev. Cell 12 Pages: 113-127
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Renal damage in obstructive nephropathy is decreased in Skp2-deficient mice (2007)
  • Author(s): Suzuki, S., Fukasawa, H., Kitagawa, K., Uchida, C, Hattori, T., Isobe, T., Oda, T., Misaki, T., Ohashi, N., Nakayama, K., Nakayama, K.I., Hishida, A., Yamamoto, T., Kitagawa, M.
  • Journal Title: Am. J. Pathol. 171 Pages: 473-483
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Cyclin D2 Translocates p27 out of the Nucleus and Promotes Its Degradation at the G0-G1 Transition (2007)
  • Author(s): Susaki, E., Nakayama K., Nakayama, K. I.
  • Journal Title: Mol. Cell. Biol. 27 Pages: 4626-4640
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Skp2 controls adipocyte proliferation during the development of obesity (2007)
  • Author(s): Sakai, T., Sakaue, H., Nakamura, T., Okada, M., Matsuki, Y., Watanabe, E., Hiramatsu, R., Nakayama, K., Nakayama, K.I. and Kasuga, M.
  • Journal Title: J. Biol. Chem. 282 Pages: 2038-2046
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Asbestos-induced peribronchiolar cell proliferation and cytokine production are attenuated in lungs of protein kinase C-delta knockout mice (2007)
  • Author(s): Shukla, A., Lounsbury, K. M., Barrett, T. F., Gell, J., Rincon, M., Butnor, K. J., Taatjes, D. J., Davis, G. S., Vacek, P., Nakayama, K. I., Nakayama K., Steele C., Mossman, B. T.
  • Journal Title: Am. J. Pathol. 170 Pages: 140-151
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Conditional inactivation of Fbxw7 impairs cell-cycle exit during T cell differentiation and results in lymphomatogenesis (2007)
  • Author(s): Onoyama, I., R. Tsunematsu, A. Matsumoto, T. Kimura, I. M. de Alboran, K. Nakayama, K. I. Nakayama
  • Journal Title: J. Exp. Med 204 Pages: 2875-2888
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Foxo3a is essential for maintenance of the hematopoietic stem cell pool (2007)
  • Author(s): Miyamoto, K., Araki, K.Y., Naka, K., Arai, F., Takubo, K., Yamazaki, S., Matsuoka, S., Miyamoto, T., Ito, K., Ohmura, M., Chen, C., Hosokawa, K., Nakauchi, H., Nakayama, K., Nakayama, K.I., Harada, M., Motoyama, N., Suda, T. and Hirao, A.
  • Journal Title: Cell Stem Cell 1 Pages: 101-112
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Davis. nPKC{varepsilon}, a P2Y2-R downstream effector in regulated mucin secretion from airway goblet cells (2007)
  • Author(s): Ehre, C, Y. Zhu, L. H. Abdullah, J. Olsen, K. I. Nakayama, K. Nakayama, R. O. Messing, C. W.
  • Journal Title: Am. J. Physiol. -Cell Physiol. 293 Pages: 1445-1454
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] The F Box Protein S Phase Kinase-associated Protein 2 Regulates Adipose Mass and Adipocyte Number in Vivo (2007)
  • Author(s): Cooke, P. S., Holsberger, D. R., Cimafranca, M. A., Meling, D. D., Beals, C. M., Nakayama, K., Nakayama, K. I., Kiyokawa, H.
  • Journal Title: Obesity 15 Pages: 1400-1408
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Protein kinase C-delta phosphorylates Ebp1 and prevents its proteolytic degradation, enhancing cell survival (2007)
  • Author(s): Liu, Z., Liu, X., Nakayama, K. I., Nakayama, K., Ye, K.
  • Journal Title: J. Neurochem. 100 Pages: 1278-1288
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] The cyclin-dependent kinase inhibitors p57 and p27 regulate neuronal migration in the developing mouse neocortex (2007)
  • Author(s): Itoh, Y., Masuyama, N., Nakayama, K., NakayamaK. I., Gotoh, Y.
  • Journal Title: J. Biol. Chem 282 Pages: 390-396
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] SCFbeta TRCP1 activates and ubiquitylates TAp63gamma (2007)
  • Author(s): J. R. Gallegos, et. al.
  • Journal Title: J Biol Chem 283 Pages: 66-75
  • Peer Reviewed
• [Journal Article] Essential role of Skp2-mediated p27 degradation in growth and adaptive expansion of pancreatic beta cells, (2007)
  • Author(s): L. Zhong, et. al.
  • Journal Title: J Clin lnvest 117 Pages: 2869-2876
  • Peer Reviewed
• [Journal Article] Ablation of p27 enhance kainate-induced seizure and hippocampal Degeneration (2007)
  • Author(s): C. Ueyama, et. al.
  • Journal Title: Neuroreport 18 Pages: 1781-1785
  • Peer Reviewed
• [Journal Article] Conditional inactivation of Fbxw7 impairs cell-cycle exit during T cell differentiation and results in lymphomatogenesis (2007)
  • Author(s): l. Onoyama, et. al.
  • Journal Title: J Exp Med 204 Pages: 2875-2888
  • Peer Reviewed
• [Journal Article] nPKC(varepsilon), a P2Y2-R downstream effector in regulated mucin secretion from airway goblet cells (2007)
  • Author(s): C. Ehre, et. al.
  • Journal Title: Am J Physiol Cell Physiol 293
  • Peer Reviewed
• [Journal Article] Protein kinase C-delta phosphorylates Ebpl and prevents its proteolytic degradation, enhancing cell survival (2007)
  • Author(s): Z. Liu, et. al.
  • Journal Title: J Neurochem 100 Pages: 1278-1288
  • Peer Reviewed
• [Journal Article] Negative Feedback Loop in Bim-Caspase-3 Axis Regulating Apoptosis and Activity of Osteoclasts (2007)
  • Author(s): H. Wakeyama, et. al.
  • Journal Title: J Bone Miner Res 22 Pages: 1631-1639
  • Peer Reviewed
• [Journal Article] The F Box Protein S Phase Kinase-associated Protein 2 Regulates Adipose Mass and Adipocyte Number in Vivo, (2007)
  • Author(s): P. S. Cooke, et. al.
  • Journal Title: Obesity(Silver Spring) 15 Pages: 1400-1408
  • Peer Reviewed
• [Journal Article] Cyclin D2 Translocates p27 out of the Nucleus and Promotes Its Degradation at the G0-G1 Transition (2007)
  • Author(s): E. Susaki, et. al
  • Journal Title: Mol Cell Biol 27 Pages: 4626-4640
  • Peer Reviewed
• [Journal Article] Skp2 controls adipocyte proliferation during the development ofobesity (2007)
  • Author(s): T. Sakai, et. al.
  • Journal Title: J Biol Chem 282 Pages: 2038-2046
  • Peer Reviewed
• [Journal Article] The cyclin-dependent kinase inhibitors p57 and p27 regulate neuronal migration in the developing mouse neocortex (2007)
  • Author(s): Y. Itoh, et. al.
  • Journal Title: J Biol Chem 282 Pages: 390-396
  • Peer Reviewed
• [Journal Article] Noncanonical Wnt signaling through G protein-linked PKC delta activation promotes bone formation (2007)
  • Author(s): X. Tu, et. al.
  • Journal Title: Dev Cell 12 Pages: 113-127
  • Peer Reviewed
• [Journal Article] Asbestos-induced peribronchiolar cell proliferation and cytokine prodc-tion are attenuated in lungs of protein kinase C-delta knockout mice (2007)
  • Author(s): A. Shukla, et. al.
  • Journal Title: Am J Pathol 170 Pages: 140-151
  • Peer Reviewed
• [Journal Article] Renal damage in obstructive nephropathy is decreased in Skp2-deficient mice (2007)
  • Author(s): S. Suzuki, et. al.
  • Journal Title: Am J Pathol 171 Pages: 473-483
  • Peer Reviewed
• [Journal Article] PKC-delta plays non-redundant role in insulin secretion in pancreatic beta cell. (2007)
  • Author(s): Uchida, T., et al.
  • Journal Title: J. Biol. Chem. 282 Pages: 2707-2716
• [Journal Article] Asbestos-induced peribronchiolar cell proliferation and cytokine production are attenuated in lungs of protein kinase C-{delta} knockout mice. (2007)
  • Author(s): Shukla, A., et al.
  • Journal Title: Am. J. Pathol. 170 Pages: 140-151
• [Journal Article] Noncanonical Wnt signaling through G protein-linked PKCdelta activation promotes bone formation. (2007)
  • Author(s): Tu, X., et al.
  • Journal Title: Dev. Cell 12 Pages: 113-127
• [Journal Article] The cyclin-dependent kinase inhibitors p57 and p27 regulate neuronal migration in the developing mouse neocortex. (2007)
  • Author(s): Itoh, Y., et al.
  • Journal Title: J. Biol. Chem. 282 Pages: 390-396
• [Journal Article] Skp2 controls adipocyte proliferation during the development of obesity. (2007)
  • Author(s): Sakai, T., et al.
  • Journal Title: J. Biol. Chem. 282 Pages: 2038-2046
• [Journal Article] Geminin is essential for the development of preimplantation mouse embryos. (2006)
  • Author(s): Hara, K., et. al.
  • Journal Title: Genes Cells 11 Pages: 1281-11293
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] PKCδ regulates collagen-induced platelet aggregation through inhibition of VASP-mediated filopodia formation (2006)
  • Author(s): Pula, G., et. al.
  • Journal Title: Blood 108 Pages: 4035-4044
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Mitogenic signalling and the p16INK4a/Rb pathway co-operate to enforce irreversible cellular senescence through activating ROS/PKC-δ signalling pathway. (2006)
  • Author(s): Takahashi, A., et. al.
  • Journal Title: Nature Cell Biol. 8 Pages: 1291-1297
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Double knockout mice show BASH and PKCδ have different epistatic relationships in B cell maturation and CD40-mediated activation. (2006)
  • Author(s): Nojima, T., et. al.
  • Journal Title: Immunol. Lett. 105 Pages: 48-54
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Ubiquitin ligases controlling cell cycle and cancer. (2006)
  • Author(s): Nakayama, K.I., et. al.
  • Journal Title: Nat. Rev. Cancer 6 Pages: 369-381
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] HSP27 favors ubiquitination and proteasomal degradation of p27Kip1 and helps S-phase re-entry in stressed cells. (2006)
  • Author(s): Parcellier, A., et. al.
  • Journal Title: FASEB J. 20 Pages: 1179-1181
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Suppression of centrosome amplification after DNA damage depends on p27 accumulation. (2006)
  • Author(s): Sugihara, E., et. al.
  • Journal Title: Cancer Res., 66, , 2006 66 Pages: 4020-4029
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Identification of novel E2F1 target genes regulated in cell cycle-dependent and independent manners. (2006)
  • Author(s): Iwanaga, R., et. al.
  • Journal Title: Oncogene, 25, 2006 25 Pages: 1786-1798
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] wo E3 ubiquitin ligases, SCF-Skp2 and DDB1-Cul4, target human Cdt1 for aroteolysis. (2006)
  • Author(s): Nishitani, H., et. al.
  • Journal Title: EMBO J., 25, , 2006 25 Pages: 1126-1136
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] PKCdelta is necessary for Smad3 expression and transforming growth factorbeta-induced fibronectin synthesis in vascular smooth muscle cells. (2006)
  • Author(s): Ryer, E. J., et. al.
  • Journal Title: Arterioscler. Thromb. Vasc. Biol. 26 Pages: 780-786
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Transcriptional up-regulation of MMP12 and MMP13 by asbestos occurs via a PKCδ-dependent pathway in murine lung. (2006)
  • Author(s): Shukla, A., et. al.
  • Journal Title: FASEB J. 20 Pages: 997-999
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Up-regulation of GPR48 induced by down-regulation of p27Kip1 enhances carcinoma cell invasiveness and metastasis. (2006)
  • Author(s): Gao, Y., et. al.
  • Journal Title: Cancer Res. 66 Pages: 11623-11631
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Impaired germ cell development due to compromised cell cycle progression in Skp2-deficient mice. (2006)
  • Author(s): Fotovati, A., et. al.
  • Journal Title: Cell Div. 1 Pages: 4-4
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Geminin is essential for the development of preimplantation mouse embryos (2006)
  • Author(s): Hara, K., Nakayama, K.I., Nakayama, K.
  • Journal Title: Genes Cells 11 Pages: 1281-1293
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] PKCδ regulates collagen-induced platelet aggregation through inhibition of VASP-mediated filopodia formation (2006)
  • Author(s): Pula, G., Schuh, K., Nakayama K., Nakayama, K. I., Walter, U., Poole, A. W.
  • Journal Title: Blood 108 Pages: 4035-4044
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Mitogenic signalling and the p16INK4a/Rb pathway co-operate to enforce irreversible cellular senescence through activating ROS/PKC-5 signalling pathway (2006)
  • Author(s): Takahashi, A., Ohtani, N., Yamakoshi, K., lida, S., Tahara, H., Nakayama, K., Nakayama, K.I., Ide, T., Saya, H., Hara, E.
  • Journal Title: Nature Cell Biol. 8 Pages: 1291-1297
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Double knockout mice show BASH and PKCδhave different epistatic relationships in B cell maturation and CD40-mediated activation (2006)
  • Author(s): Nojima, T., Hayashi, K., Goitsuka, R., Nakayama, K., Nakayama, K.l., Kitamura, D.
  • Journal Title: Immunol. Lett. 105 Pages: 48-54
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Ubiquitin ligases controlling cell cycle and cancer (2006)
  • Author(s): Nakayama, K.I., Nakayama, K.
  • Journal Title: Nat. Rev. Cancer 6 Pages: 369-381
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] HSP27 favors ubiquitination and proteasomal degradation of p27Kip1 and helps S-phase re-entry in stressed cells (2006)
  • Author(s): Parcellier, A., Brunet, M., Schmitt, E., Col, E., Didelot, C., Hammann, A., Nakayama, K., Nakayama, KI., Khochbin, S., Solary, E.., Garrido, C.
  • Journal Title: FASEB J. 20 Pages: 1179-1181
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Suppression of centrosome amplification after DNA damage depends on p27 accumulation (2006)
  • Author(s): Sugihara, E., Kanai, M., Saito, S., Nitta, T., Toyoshima, H., Nakayama, K., Nakayama, K.I., Fukasawa, K., Schwab, M., Saya, H., Miwa, M.
  • Journal Title: Cancer Res. 66 Pages: 4020-4029
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Identification of novel E2F1 target genes regulated in cell cycle-dependent and independent manners (2006)
  • Author(s): Iwanaga, R., Komori, H., Ishida, S., Okamura, N., Nakayama, K., Nakayama, K. I., Ohtani, K.
  • Journal Title: Oncogene 25 Pages: 1786-1798
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Two E3 ubiquitin ligases, SCF-Skp2 and DDB1-Cul4, target human Cdt1 for proteolysis (2006)
  • Author(s): Nishitani, H., Sugimoto, N., Roukos, V., Nakanishi, Y., Saijo, M., Obuse, C., Tsurimoto, T., Nakayama, K. I., Nakayama, K., Fujita, M., Lygerou, Z., Nishimoto, T.
  • Journal Title: EMBO J. 25 Pages: 1126-1136
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] PKCdelta is necessary for Smad3 expression and transforming growth factorbeta-induced fibronectin synthesis in vascular smooth muscle cells (2006)
  • Author(s): Ryer, E. J., Hom, R. P., Sakakibara, K., Nakayama, K. I., Nakayama, K., Faries, P. L, Liu, B., Kent, K. C.
  • Journal Title: Arterioscler. Thromb. Vase. Biol. 26 Pages: 780-786
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Transcriptional up-regulation of MMP12 and MMP13 by asbestos occurs via a PKCδ-dependent pathway in murine lung (2006)
  • Author(s): Shukla, A., Barrett, T.F., Nakayama, K.I., Nakayama, K., Mossman, B.T. and Lounsbury, K.M.
  • Journal Title: FASEB J. 20 Pages: 997-999
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Up-regulation of GPR48 induced by down-regulation of p27Kip1 enhances carcinomacell invasiveness and metastasis (2006)
  • Author(s): Gao, Y., Kitagawa, K., Hiramatsu, Y., Kikuchi, H., Isobe, T., Shimada, M., Uchida, C, Hattori, T., Oda, T., Nakayama, K., Nakayama, K.I., Tanaka, T., Konno, H., Kitagawa, M.
  • Journal Title: Cancer Res. 66 Pages: 11623-11631
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Impaired germ cell development due to compromised cell cycle progression in Skp2-deficient mice (2006)
  • Author(s): Fotovati, A., Nakayama, K., Nakayama, K.I.
  • Journal Title: Cell Div. 1 Pages: 4-4
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Suppression of centrosome amplification after DNA damage depends on p27 accumulation. (2006)
  • Author(s): Sugihara, E., et al.
  • Journal Title: Cancer Res. 66 Pages: 4020-4029
• [Journal Article] PKCdelta is necessary for Smad3 expression and transforming growth factor beta-induced fibronectin synthesis in vascular smooth muscle cells. (2006)
  • Author(s): Ryer, E.J., et al.
  • Journal Title: Arterioscler. Thromb. Vasc. Biol. 26 Pages: 780-786
• [Journal Article] HSP27 favors ubiquitination and proteasomal degradation of p27Kip1 and helps S-phase re-entry in stressed cells. (2006)
  • Author(s): Parcellier, A., et al.
  • Journal Title: FASEB J. 20 Pages: 1179-1181
• [Journal Article] Impaired germ cell development due to compromised cell cycle progression in Skp2-deficient mice. (2006)
  • Author(s): Fotovati, A., et al.
  • Journal Title: Cell Div. 1 Pages: 4-4
• [Journal Article] Transcriptional up-regulation of MMP12 and MMP13 by asbestos occurs via a PKCdelta-dependent pathway in murine lung. (2006)
  • Author(s): Shukla, A., et al.
  • Journal Title: FASEB J. 20 Pages: 997-999
• [Journal Article] Geminin is essential for the development of preimplantation mouse embryos. (2006)
  • Author(s): Hara, K., et al.
  • Journal Title: Genes Cells 11 Pages: 1281-1293
• [Journal Article] Mitogenic signalling and the p16INK4a-Rb pathway cooperate to enforce irreversible cellular senescence. (2006)
  • Author(s): Takahashi, A., et al.
  • Journal Title: Nature Cell Biol. 8 Pages: 1291-1297
• [Journal Article] Up-regulation of GPR48 induced by down-regulation of p27Kip1 enhances carcinoma cell invasiveness and metastasis. (2006)
  • Author(s): Gao, Y., et al.
  • Journal Title: Cancer Res. 66 Pages: 11623-11631
• [Journal Article] PKCdelta regulates collagen-induced platelet aggregation through inhibition of VASP-mediated filopodia formation. (2006)
  • Author(s): Pula, G., et al.
  • Journal Title: Blood 108 Pages: 4035-4044
• [Journal Article] Double knockout mice show BASH and PKCd have different epistatic relationships in B cell maturation and CD40-mediated activation. (2006)
  • Author(s): Nojima, T., et al.
  • Journal Title: Immunol. Lett. 105 Pages: 48-54
• [Presentation] Gdx, an X-Linked Ubiquitin-Like Modifier, ls Conjugated to Cyclin F and Regulates Mitosis (2008)
  • Author(s): N. lshida, et. al.
  • Organizer: 4th lnternational Conference, Ubiquitin, Ubiquitin-Like Proteins, and Cancer
  • Place of Presentation: Huston, TX, USA
  • Year and Date: 2008-07-02
• [Presentation] Gdx, an X-Linked Ubiquitin-Like Modifier, ls Conjugated to Cyclin Fand Regulates Mitosis. (2008)
  • Author(s): lshida, N., et. al.
  • Organizer: 4th lnternational Conference, Ubiquitin, Ubiquitin-Like Proteins, and Cancer
  • Place of Presentation: Huston, TX, USA
  • Year and Date: 2008-02-07
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Ubiquitin ligases required for the regulation of G0-G1 transision. (2007)
  • Author(s): Nakayama, K., et. al.
  • Organizer: Office of Life Sciences/National University of Singapore and Tohoku University/Center of Excellence Joint Syposium
  • Place of Presentation: Singapore
  • Year and Date: 2007-05-09
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 腎障害進行におけるSkp2発現亢進の解析:Skp2KOマウスを用いた検討 (2007)
  • Author(s): 鈴木小由理, et. al.
  • Organizer: 第30回日本分子生物学会年会・第80回日本生化学会合同大会
  • Place of Presentation: 横浜
• [Presentation] 新規ユビキチン様修飾分子GdXによるcyclin Fと細胞周期M期の制御 (2007)
  • Author(s): 石田典子, et. al.
  • Organizer: 第30回日本分子生物学会年会・第80回日本生化学会合同大会
  • Place of Presentation: 横浜
• [Presentation] ユビキチンリガーゼFbw7は表皮角化細胞において増殖と分化を制御する (2007)
  • Author(s): 石川善則, et. al.
  • Organizer: 第30回日本分子生物学会年会・第80回日本生化学会合同大会
  • Place of Presentation: 横浜
• [Presentation] Fbxw7によるGO期維持機構とその破綻による発がん (2007)
  • Author(s): 小野山一郎, et. al.
  • Organizer: 第30回日本分子生物学会年会・第80回日本生化学会合同大会
  • Place of Presentation: 横浜
• [Presentation] Gemininは着床前初期発生に必須である,第30回日本分子生物学会年会 (2007)
  • Author(s): 原賢太郎, et. al.
  • Organizer: 第30回日本分子生物学会年会・第80回日本生化学会合同大会
  • Place of Presentation: 横浜
• [Presentation] Control of Cell Cycle and Chromosomal Stability. (2006)
  • Author(s): Nakayama, K., et. al.
  • Organizer: The 3rd International Symposium on "Novel perspecfives in cancer research and translationto the clinic
  • Place of Presentation: Sendai,Japan
  • Year and Date: 2006-11-09
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Geminin is essential for the development of preimplantation mouse embryos. (2006)
  • Author(s): Hara, K., et. al.
  • Organizer: The 3rd International Symposium on "Novel perspectives in cancer research and translation to the clinic,
  • Place of Presentation: Sendai, Japan
  • Year and Date: 2006-11-09
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] FBW7 is a key regulator of Cell Cycle arrest and Tumorigenesis. (2006)
  • Author(s): Nakayama, K.
  • Organizer: The 3rd International Symposium on "Novel perspectives in cancer research and translation to the clinic,
  • Place of Presentation: Sendai, Japan
  • Year and Date: 2006-11-09
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] GdX, an X-linked ubiquitin-like modifier, is conjugated to cyclin F and regulates cell cycle. (2006)
  • Author(s): Ishida, N., et. al.
  • Organizer: The 3rd International Symposium on "Novel perspectives in cancer research and translation to the clinic,
  • Place of Presentation: Sendai, Japan
  • Year and Date: 2006-11-09
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Accumulation of Notch induces cell cycle arrest in the tumor suppressor Fbxw7-deficient MEFs. (2006)
  • Author(s): Ishikawa, Y., et. al.
  • Organizer: The 3rd International Symposium on "Novel perspectives in cancer research and translation to the clinic
  • Place of Presentation: Sendai, Japan
  • Year and Date: 2006-11-09
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Regulation of CDK inhibitors by ubiquitin ligase Fbxw7. (2006)
  • Author(s): Ishikawa Y., et. al.
  • Organizer: 20th IUBMB International Congress of Biochemistry and Molecular Biology
  • Place of Presentation: Kyoto, Japan
  • Year and Date: 2006-06-21
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] GdX, An X-Linked ubiquifin-like modifier, is conjugated to cyclin Fand regulates mitotic exit. (2006)
  • Author(s): Ishida, N., et. al.
  • Organizer: 20th IUBMB International Congress of Biochemistry and Molecular Biology
  • Place of Presentation: Kyoto, Japan
  • Year and Date: 2006-06-21
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Geminin is essential for development of preimplantation embryos (2006)
  • Author(s): Hara, K
  • Organizer: 20th IUBMB International Congress of Biochemistry and Molecular Biology
  • Place of Presentation: Kyoto, Japan
  • Year and Date: 2006-06-21
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Suppression of centrosome amplification after DNA damage depends on p27 accumulation. (2006)
  • Author(s): Sugihara, E
  • Organizer: 20th IUBMB International Congress of Biochemistry and Molecular Biology
  • Place of Presentation: Kyoto, Japan
  • Year and Date: 2006-06-21
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Fbw7 contributes to cell-cycle regulation and tumor suppression during development. (2006)
  • Author(s): Onoyama, I.
  • Organizer: 20th IUBMB International Congress of Biochemistry and Molecular Biology
  • Place of Presentation: Kyoto, Japan
  • Year and Date: 2006-06-21
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] The role of FWD1a and FWD1b as circadian rhythm formation. (2006)
  • Author(s): Ohsaki, K.
  • Organizer: 20th IUBMB International Congress of Biochemistry and Molecular Biology
  • Place of Presentation: Kyoto, Japan
  • Year and Date: 2006-06-21
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] p27 can substitute for p57 in mouse development. (2006)
  • Author(s): Susaki, E
  • Organizer: Cold Spring Harbor Symposium "The Cell Cycle"
  • Place of Presentation: Cold Spring Harbor, NY
  • Year and Date: 2006-05-19
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] RASSF1A is required for Skp2-mediated G1-S transition and stabilization of p53. (2006)
  • Author(s): Song, M. S
  • Organizer: Cold Spring Harbor Symposium "The Cell Cycle"
  • Place of Presentation: Cold Spring Harbor, NY
  • Year and Date: 2006-05-19
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Fbw7 is a key regulator of cell-cycle arrest and tumorigenesis during development (2006)
  • Author(s): Onoyama, I.
  • Organizer: Cold Spring Harbor Symposium ″The Cell Cycle″
  • Place of Presentation: Cold Spring Harbor, NY
  • Year and Date: 2006-05-19
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Geminin is essential for development of preimplantation embryos (2006)
  • Author(s): Hara, K.
  • Organizer: Cold Spring Harbor Symposium "The Cell Cycle"
  • Place of Presentation: Cold Spring Harbor, NY
  • Year and Date: 2006-05-19
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] RASSF1A is required for Skp2-mediated G1-S transition and stabilization of p53 (2006)
  • Author(s): Song, M.S., Song, S.J., Kim, S.Y., Yang, T.-H., Pfeifer, G.P., Nakayama, K., Nakayama, K.I. and Lim, D.-S.
  • Organizer: Cold Spring Harbor Symposium"The Cell Cycle"
  • Place of Presentation: Cold Spring Harbor, NY
  • Year and Date: 2006-05-19
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Fbw7 is a key regulator of cell-cycle arrest and tumorigenesis during development (2006)
  • Author(s): Onoyama, I., Tsunematsu, R., Nakayama, K. and Nakayama, K.I.
  • Organizer: Cold Spring Harbor Symposium"The Cell Cycle"
  • Place of Presentation: Cold Spring Harbor, NY
  • Year and Date: 2006-05-19
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Geminin is essential for development of preimplantation embryos (2006)
  • Author(s): Hara, K., Nakayama, K. and Nakayama, K.
  • Organizer: Cold Spring Harbor Symposium"The Cell Cycle"
  • Place of Presentation: Cold Spring Harbor, NY
  • Year and Date: 2006-05-19
  • Description: 「研究成果報告書概要(欧文)」より