Neuro-vascular patterning determined by segmentation

• Project/Area Number: 18370087
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Developmental biology
• Research Institution: Nara Institute of Science and Technology
• Principal Investigator: TAKAHASHI Yoshiko Nara Institute of Science and Technology, バイオサイエンス研究科, 教授 (10183857)
• Project Period (FY): 2006 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥18,080,000 (Direct Cost: ¥15,200,000、Indirect Cost: ¥2,880,000); Fiscal Year 2008: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000); Fiscal Year 2007: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000); Fiscal Year 2006: ¥5,600,000 (Direct Cost: ¥5,600,000)
• Keywords: 分節 / Notch / 細胞移動 / エフリン / 神経 / 血管形成 / Eph / Ephrin / 体節 / 背側大動脈 / Tol2 / 細胞死 / エレクトロポレーション法 / Tol2トランスポゾン

Research Abstract

脊椎動物の初期発生過程において出現する体節中胚葉の分節化は、体節以外の組織、たとえば神経系や血管系などの分節パターンをも規定する. 本研究では、分節パターンに沿って出現する血管前駆細胞に注目し、これらの細胞による血管形成の過程と、それを制御する分子メカニズムの解析を行った.血管前駆細胞は各体節の後半部に出現し、その後腹側方向に移動して背側大動脈の内皮組織を形成する. これらの細胞挙動は、主にNotchとその下流で働くエフリンによって制御されていることが明らかとなった.

Research Products

• [Journal Article] Ephrin B2 coordinates the formation of a morphological boundary and cell epithelialization during somite segmentation (2009)
  • Author(s): Watanabe, T., Sato, Y., Saito, D., Tadokoro, R., Takahashi, Y.
  • Journal Title: Proc. Natl. Acad. Sci. USA. Pages: 7467-7472
  • Peer Reviewed
• [Journal Article] Exploration of embryonic origins of germline stem cells and neoblasts in Enchytraeus japonensis (2008)
  • Author(s): Sugio, M., Takeuchi, K., Kutsuna, J., Tadokoro, R., Takahashi, Y., Yoshida-Noro C, Tochinai S.
  • Journal Title: Gene Expression Patterns Vol.8 Pages: 227-236
  • Peer Reviewed
• [Journal Article] Notch mediates the segmental specification of angioblasts in somites and their directed migration toward the dorsal aorta in avian embryos (2008)
  • Author(s): Sato, Y., Watanabe, T., Saito, D., Takahashi, T., Yoshida, S., Kohyama, J., Ohata, E., Okano, H., Takahashi Y.
  • Journal Title: Developmental Cell Vol.14 Pages: 890-901
  • Peer Reviewed
• [Journal Article] Somitogenesis as a model to study the formation of morphological boundaries and cell epithelialization (2008)
  • Author(s): Takahashi, Y., Sato, Y.
  • Journal Title: Dev. Growth Differ Vol.50 Pages: 149-155
  • NAID: 10024399769
  • Peer Reviewed
• [Journal Article] Transposon-mediated stable integration and tetracycline-inducible expression of electroporated transgenes in chicken embryos (2008)
  • Author(s): Takahashi, Y.(他4名)
  • Journal Title: Methods in Cell Biology Vol.87 Pages: 237-256
  • Peer Reviewed
• [Journal Article] Transposon-mediated stable integration and tetracycline-inducible expression of electroporated transgenes in chicken embryos, Methods in Avian Embryology (2008)
  • Author(s): Takahashi, Y.(他4名)
  • Journal Title: Methods in Cell Biology Vol.87
  • Peer Reviewed
• [Journal Article] Transposon-mediated stable integration and tetracycline-inducibleexpression of electroporated transgenes in chicken embryos (2008)
  • Author(s): Takahashi, Y.
  • Journal Title: Methods in Cell Biology 87 Pages: 237-256
  • Peer Reviewed
• [Journal Article] Somitogenesis as a model to study the formation of morphologicalboundaries and cell epithelialization (2008)
  • Author(s): Takahashi, Y.
  • Journal Title: Dev. Growth & Differ. 50 Pages: 149-155
  • Peer Reviewed
• [Journal Article] Notch mediates the segmental specification of angioblasts in somitesand their directed migration toward the dorsal aorta in avian embryos (2008)
  • Author(s): Sato, Y.
  • Journal Title: Developmental Cell 14 Pages: 890-901
  • Peer Reviewed
• [Journal Article] Exploration of embryonic origins of germline stem cells and neoblastsin Enchytraeus japonensis (2008)
  • Author(s): Sugio, M.
  • Journal Title: Gene Expression Patterns 8 Pages: 227-236
  • Peer Reviewed
• [Journal Article] Exploration of embryonic origins of germline stem cells and neoblasts in Enchytraeus japonensis(Oligochaeta, Annelida). (2008)
  • Author(s): Sugio, M.
  • Journal Title: GEP 8 Pages: 227-36
  • Peer Reviewed
• [Journal Article] L-Maf regulates p27kip1 expression during chick lens fiber differentiation (2007)
  • Author(s): Reza, H. M., Nishi, H., Kataoka, K., Takahashi, Y., Yasuda, K.
  • Journal Title: Differentiation Vol.75 Pages: 737-744
  • Peer Reviewed
• [Journal Article] Stage-dependent expression of Pax6 in optic vesicle/cup regulates patterning genes through signaling molecules (2007)
  • Author(s): Reza, H. M., Takahashi, Y., Yasuda, K.
  • Journal Title: Differentiation Vol.75 Pages: 726-736
  • Peer Reviewed
• [Journal Article] Tet-on inducible system combined with in ovo electroporation dissects multiple roles of genes in somitogenesis of chicken embryos (2007)
  • Author(s): Watanabe, T., Saito, D., Tanabe, K., Suetsugu, R., Nakaya, Y., Nakagawa, S. Takahashi, Y.
  • Journal Title: Developmental Biology Vol.305 Pages: 625-636
  • Peer Reviewed
• [Journal Article] Stable integration and conditional expression of electroporated transgenes in chicken embryos (2007)
  • Author(s): Sato, T., Kasai, Nakagawa, S., Tanabe, J., Watanabe, T., Kawakami, K., Takahashi, Y.
  • Journal Title: Developmental Biology Vol.305 Pages: 616-624
  • Peer Reviewed
• [Journal Article] Tet-on inducible system combined with in ovo electroporation dissects multiple roles of genes in somitogenesis of chicken embryos (2007)
  • Author(s): Watanabe, T., Saito, D., Tanabe, K., Suetsugu, R., Nakaya, Y., Nakagawa, S., Takahashi, Y.
  • Journal Title: Developmental Biology Vol.305 Pages: 625-636
  • Peer Reviewed
• [Journal Article] Stable integration and conditional expression of electroporated transgenes in chicken embryos (2007)
  • Author(s): Sato, T., Kasai, T., Nakagawa, S., Tanabe, J., Watanabe, T., Kawakami, K., Takahashi, Y.
  • Journal Title: Developmental Biology Vol.305 Pages: 616-624
  • Peer Reviewed
• [Journal Article] L-Maf regulates p27kipl expression during chick lens fiber differentiation. (2007)
  • Author(s): Reza, H. M.
  • Journal Title: Differentiation 75 Pages: 737-744
  • Peer Reviewed
• [Journal Article] Stage-dependent expression of Pax6 in optic vesicle/cup regulates patterning genes through signaling molecules. (2007)
  • Author(s): Reza, H. M.
  • Journal Title: Differentiation 75 Pages: 726-736
  • Peer Reviewed
• [Journal Article] Tet-on inducible system combined with in ovo electroporation dissects multiple roles of genes in somitogenesis of chicken embryos. (2007)
  • Author(s): Watanabe, T.
  • Journal Title: Dev. Biol. 305 Pages: 625-636
  • Peer Reviewed
• [Journal Article] Stable integration and conditional expression of electroporated transgenes in chicken embryos. (2007)
  • Author(s): Sato, Y.
  • Journal Title: Dev. Biol. 305 Pages: 616-624
  • Peer Reviewed
• [Journal Article] Stable integration and conditional expression of electroporated transgenes in chicken embryos (2007)
  • Author(s): Sato, Y., Kasai, I., Nakagawa, S., Tanabe, K., Watanabe, T., Kawakami, K. and Takahashi, Y.
  • Journal Title: Developmental Biology 305 Pages: 625-636
• [Journal Article] Tet-on inducible system combined with in ovo electroporation dissects multiple roles of genes in somitogenesis of chicken embryos (2007)
  • Author(s): Watanabe, T., Saito, D., Tanabe, K., Suetsugu, R., Nakaya, Y., Nakagawa, S. and Takahashi, Y.
  • Journal Title: Developmental Biology 305 Pages: 625-636
• [Journal Article] Cadherin is required for dendritic morphogenesis and synaptic terminal organization of retinal horizontal cells (2006)
  • Author(s): Tanabe, K., Takahashi, Y., (他4名)
  • Journal Title: Development Vol.133 Pages: 4085-4096
  • Peer Reviewed
• [Journal Article] Early segregation of germ and somatic lineages during gonadal regeneration in the annelid (2006)
  • Author(s): Tadokoro, R., Sugio, M., Kutsuna, J., Tochinai, S., Takahashi, Y.
  • Journal Title: Enchytraeus japonensis, Current Biology Vol.16 Pages: 1012-1017
  • Peer Reviewed
• [Journal Article] Level-specific role of paraxial mesoderm in regulation of Tbx5/Tbx4 expression and limb initiation (2006)
  • Author(s): Saito, D., Yonei-Tamura,S., Takahashi, Y. , Tamura, K.
  • Journal Title: Developmental Biology Vol.292 Pages: 79-89
  • Peer Reviewed
• [Journal Article] Epimorphin acts extracellularly to promote cell-sorting and aggregation during the condensation of vertebral cartilage (2006)
  • Author(s): Oka, Y., Sato, Y., Tsuda, H., Hanaoka, K., Hirai, Y., Takahashi, Y.
  • Journal Title: Developmental Biology Vol.291 Pages: 25-37
  • Peer Reviewed
• [Journal Article] Embryonic stem cells that differentiate into RPE cell precursors in vitro develop into RPE cell monolayers in vivo (2006)
  • Author(s): Aoki, H., Hara, A., Nakagawa, S., Motohashi, T., Hirano, M., Takahashi, Y., Kunisada, T.
  • Journal Title: Experimental Eye Research Vol.82 Pages: 265-74
  • Peer Reviewed
• [Journal Article] Ephrin B2 coordinates the formation of a morphological boundary andcell epithelialization during somite segmentation
  • Author(s): Watanabe, T.
  • Journal Title: Proc. Natl. Acad. Sci. USA (In press)
  • Peer Reviewed
• [Journal Article] Notch signaling_mediates the_segmental specification of angioblasts in somites and their directed migration toward the dorsal aorta in avian embryos.
  • Author(s): Sato, Y.
  • Journal Title: Developmental Cell (in press)
  • Peer Reviewed
• [Journal Article] Transposon-mediated stable integration and tetracycline-inducible expression of electroporated transgenes in chicken embryos.
  • Author(s): Takahashi, Y.
  • Journal Title: Methods in Avian Embryology 87(in press)
  • Peer Reviewed
• [Journal Article] Somitogenesis as a model to study the formation of morphological boundaries and cell epithelialization.
  • Author(s): Takahashi, Y.
  • Journal Title: Dev. Growth Differ. (in press)
  • NAID: 10024399769
  • Peer Reviewed
• [Presentation] Nural crest cell migration and contribution to the adrenal glands is regulated by multiple factors mediated by CXCR4/SDF1, BMP4, and transcription factor SF-1, BMB2008 (2008)
  • Author(s): 高橋淑子
  • Organizer: 第31回日本分子生物学会年会・第81回日本生化学会大会 合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-10
• [Presentation] Nural crest cell migration and contribution to the adrenal glands isregulated by multiple factors mediated by CXCE4/SDF1, BMP4, andtranscription factor SF-1 (2008)
  • Author(s): Takahashi, Y.
  • Organizer: BMB2008 (第31回日本分子生物学会年会・第81回日本生化学会大会合同大会)(Symposium Organizer ・特別講演)
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-10
• [Presentation] 器官形成における細胞挙動を制御する遺伝プログラム (2008)
  • Author(s): 高橋淑子
  • Organizer: 遺伝医学セミナー
  • Place of Presentation: 大阪
  • Year and Date: 2008-09-06
• [Presentation] 器官形成における細胞挙動を制御する遺伝プログラム (2008)
  • Author(s): 高橋淑子
  • Organizer: 遺伝医学セミナー(招待講演)
  • Place of Presentation: 大阪
  • Year and Date: 2008-09-06
• [Presentation] 器官形成にみる細胞の奇妙なふるまい (2008)
  • Author(s): 高橋淑子
  • Organizer: 第20回高遠・分子細胞生物学シンポジウム
  • Place of Presentation: 長野
  • Year and Date: 2008-08-21
• [Presentation] Neural crest cell migration to the adrenal gland is regulated by CXCR4 /SDF1, BMP4, and transcription factor SF-1, The 31st Annual Meeting of the Japan Neuroscience Society (2008)
  • Author(s): 高橋淑子
  • Organizer: 第31回日本神経科学大会
  • Place of Presentation: 東京
  • Year and Date: 2008-07-10
• [Presentation] 血管発生と細胞移動 (2008)
  • Author(s): 高橋淑子
  • Organizer: サントリー生物有機科学研究所
  • Place of Presentation: 大阪
  • Year and Date: 2008-06-18
• [Presentation] 血管発生と細胞移動 (2008)
  • Author(s): 高橋淑子
  • Organizer: サントリー生物有機科学研究所(招待セミナー)
  • Place of Presentation: 大阪
  • Year and Date: 2008-06-18
• [Presentation] Secondary neurulation : a novel model to study tubulogenesis (神経管形成をモデルとした上皮管構造の成り立ち), 41th Annual Meeting for the Japanese Society of Developmental Biologists (2008)
  • Author(s): 高橋淑子
  • Organizer: 第41回日本発生生物学会大会
  • Place of Presentation: 徳島
  • Year and Date: 2008-05-28
• [Presentation] 血管発生と細胞移動 (2008)
  • Author(s): 高橋淑子
  • Organizer: 日本分子生物学会第8回春季シンポジウム
  • Place of Presentation: 札幌
  • Year and Date: 2008-05-26
• [Presentation] 血管発生と細胞移動 (2008)
  • Author(s): 高橋淑子
  • Organizer: 日本分子生物学会第8回春期シンポジウム(招待講演)
  • Place of Presentation: 札幌
  • Year and Date: 2008-05-26
• [Presentation] Transposon-mediated stable integration of genes into migrating cells during blood vessel patterning (2008)
  • Author(s): 高橋淑子
  • Organizer: Experimental Biology 2008
  • Place of Presentation: USA
  • Year and Date: 2008-04-06
• [Presentation] Transposon-mediated stable integration of genes into migrating cellsduring blood vessel patterning (2008)
  • Author(s): Takahashi, Y.
  • Organizer: Experimental Biology, 2008
  • Place of Presentation: San Diego, U.S.A.
  • Year and Date: 2008-04-06
• [Presentation] Neural crest cell migration to the adrenal gland : CXCR4/SDF1 and BMP mediate guidance, while SF-1 is involved in target recognition (2008)
  • Author(s): 高橋淑子
  • Organizer: NAIST GCOE International Symposium Developmental Biology
  • Place of Presentation: 奈良
  • Year and Date: 2008-01-15
• [Presentation] 器官形成にみる細胞の奇妙なふるまい (2008)
  • Author(s): 高橋淑子
  • Organizer: 第2 0回 高遠・分子細胞生物学シンポジウム(招待講演)
  • Place of Presentation: 長野
• [Presentation] Neural crest cell migration to the adrenal gland is regulated by CXCR4/SDF1, BMP4, and transcription factor SF-1 (2008)
  • Author(s): Takahashi, Y.
  • Organizer: The 31st Annual Meeting of the Japan Neuroscience Society
  • Place of Presentation: Tokyo, Japan
• [Presentation] Secondary neurulation : a novel model to study tubulogenesis (2008)
  • Author(s): Takahashi, Y.
  • Organizer: 41th Annual Meeting for the Japanese Society of Developmental Biologists
  • Place of Presentation: Tokushima, Japan
• [Presentation] Neural crest cell migration to the adrenal gland: CXCR4/SDF1 and BMP mediate guidance, while SF-1 is involved in target recognition. (2008)
  • Author(s): Takahashi, Y.
  • Organizer: NAIST GCOE International Symposium Developmental Biology
  • Place of Presentation: Nara, Japan
• [Presentation] 体作りにおける細胞の社会~遺伝子から個体まで~ (2007)
  • Author(s): 高橋淑子
  • Organizer: 日本醸造学会大会
  • Place of Presentation: 東京
  • Year and Date: 2007-09-04
• [Presentation] 血管の発生とパターニング (2007)
  • Author(s): 高橋淑子
  • Organizer: 第5回血液・血管オルビスシンポジウム
  • Place of Presentation: 東京
  • Year and Date: 2007-08-25
• [Presentation] バイオ研究の楽しさとは~細胞の挙動を探る~ (2007)
  • Author(s): 高橋淑子
  • Organizer: 東洋紡バイオ財団設立25周年記念シンポジウム
  • Place of Presentation: 大阪
  • Year and Date: 2007-06-01
• [Presentation] バイオ研究の楽しさとは 〜細胞の挙動を探る〜 (2007)
  • Author(s): 高橋淑子
  • Organizer: 東洋紡バイオ財団設立25周年記念シンポジウム
  • Place of Presentation: 大阪
  • Year and Date: 2007-06-01
• [Presentation] Somitic contribution to the formation of dorsal aorta involves cell migration regulated by Notch and Ephrin (2007)
  • Author(s): 高橋淑子
  • Organizer: 第40回発生生物学会第59回細胞生物学会合同大会
  • Place of Presentation: 福岡
  • Year and Date: 2007-05-28
• [Presentation] Cell migration and vascular patterning in the body (2007)
  • Author(s): 高橋淑子
  • Organizer: 4th NIBB-EMBL MEETING "CELL & DEVELOPMENTAL BIOLOGY"
  • Place of Presentation: 岡崎
  • Year and Date: 2007-05-24
• [Presentation] Stable Integration and Tet-on Inducible expression of electroporated transgenes in chicken embryos (2007)
  • Author(s): 高橋淑子
  • Organizer: First International Meeting "The Chick as a model organism : genes, development and function"
  • Place of Presentation: Barcelona
  • Year and Date: 2007-04-11
• [Presentation] 体作りにおける細胞の社会 〜遺伝子から個体まで〜. (2007)
  • Author(s): 高橋淑子
  • Organizer: 日本醸造学会大会(招待講演)
  • Place of Presentation: 東京
• [Presentation] 血管の発生とパターニング. (2007)
  • Author(s): 高橋淑子
  • Organizer: 第5回血液・血管オルビスシンポジウム(特別指名講演)
  • Place of Presentation: 東京
• [Presentation] Somitic contribution to the formation of dorsal aorta involves cell migration regulated by Notch and Ephrin. (2007)
  • Author(s): 高橋淑子
  • Organizer: 第40回発生生物学会第59回細胞生物学会合同大会
  • Place of Presentation: 福岡
• [Presentation] Cell migration and vascular patterning in the body. (2007)
  • Author(s): Takahashi, Y.
  • Organizer: 4th NIBB-EMBL MEETING "CELL & DEVELOPMENTAL BIOLOGY"
  • Place of Presentation: Okazaki, Japan
• [Presentation] STABLE INTEGRATION AND TET-ON INDUCIBLE EXPRESSION OF ELECTROPORATED TRANSGENES IN CHICKEN EMBRYOS. (2007)
  • Author(s): Takahashi, Y.
  • Organizer: First International Meeting "The Chick as a model organism: genes, development and function"
  • Place of Presentation: Barcelona, Spain
• [Presentation] Cell migration and epitheliogenesis during organogenesis., Osaka-NAIST Joint COE Symposium" Cells into Organisms" (2006)
  • Author(s): 高橋淑子
  • Organizer: 細胞から個体まで
  • Place of Presentation: 奈良
  • Year and Date: 2006-06-17
• [Presentation] Vascular patterning involves dynamic migration of somatic cells mediated by chemokine, SDF-1/CXCR4 (2006)
  • Author(s): 高橋淑子
  • Organizer: 日本分子生物学会2006フォーラム「分子生物学の未来」シンポジウム「器官形成を支える細胞のふるまい」」
  • Place of Presentation: 名古屋
• [Presentation] Epithelial-mesenchymal transition is regulated by three-dimensional environment during organogenesis (2006)
  • Author(s): 高橋淑子
  • Organizer: 20th IUBMB International Congress of Biochemistry and Molecular Biology and 11th FAOBMB Congress
  • Place of Presentation: 京都
• [Presentation] Transposon-mediated gene transfer enables an exogenously introduced transgene to be stably retained and expressed in chicken embryos (2006)
  • Author(s): 高橋淑子
  • Organizer: 2006年日本発生生物学会第39回大会
  • Place of Presentation: 広島
• [Presentation] Migratory behaviors of adrenomedulla-fated neural crest cells are instructed by the adrenal cortex (2006)
  • Author(s): 高橋淑子
  • Organizer: New perspectives in Neural Crest development
  • Place of Presentation: France
• [Presentation] Early segregation of Germand somatic lineages during gonadal regeneration in the annelid, Enchytraeus japonensis., 52nd NIBB Conference "Reproductive Strategies" (2006)
  • Author(s): 高橋淑子
  • Organizer: 岡崎国際シンポジウム「生き物の生殖における戦略」
  • Place of Presentation: 岡崎
• [Book] Electroporation and Sonoporation in Developmental Biology (2009)
  • Author(s): Ohata, E., Takahashi, Y.
  • Publisher: Springer Japan
• [Book] Electroporation and Sonoporation in Developmental Biology (2009)
  • Author(s): Sato, Y., Takahashi, Y.
  • Publisher: Springer Japan
• [Book] Electroporation and Sonoporation in Developmental Biology (2009)
  • Author(s): Ohata, E.
  • Publisher: Springer Japan
• [Book] Electroporation and Sonoporation in Developmental Biology (2009)
  • Author(s): Sato, Y.
  • Publisher: Springer Japan
• [Book] 細胞工学, 細胞形成を支えるEMT(Vol.27) (2008)
  • Author(s): 吉野剛史, 下北英輔、高橋淑子
  • Publisher: 秀潤社
• [Book] 細胞工学Vol. 27 No. 4 (2008)
  • Author(s): 吉野剛史
  • Publisher: 秀潤社
• [Book] 蛋白質核酸酵素, 発生過程における細胞移動(Vol.51) (2006)
  • Author(s): 大畑絵美, 高橋淑子
  • Publisher: 共立出版
• [Book] 実験医学, からだ作りにみる上皮間充織転換(Vol.24) (2006)
  • Author(s): 吉野剛史, 仲矢由紀子, 高橋淑子
  • Publisher: 羊土社
• [Remarks] 大動脈の形成、解明(血管再生医療に道), 日本経済新聞、日経産業、産経、その他各紙、2008.6
• [Remarks] 組織を切断する遺伝子解明, 毎日新聞、日経産業、産経、その他各紙、2009.5
• [Remarks] ミミズ生殖器再生解明、日経産業新聞、朝日、毎日、その他各紙、2006.5
  • URL: http://bsw3.naist.jp/takahashi/takahashi.html