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Basic developmental strategy of chordates as revealed by the analysis of key developmental genes of ascidians

Research Project

Project/Area Number 18370088
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Developmental biology
Research InstitutionTokyo Metropolitan University

Principal Investigator

SAIGA Hidetoshi  Tokyo Metropolitan University, 理工学研究科, 教授 (60131918)

Project Period (FY) 2006 – 2008
Project Status Completed (Fiscal Year 2008)
Budget Amount *help
¥16,980,000 (Direct Cost: ¥15,000,000、Indirect Cost: ¥1,980,000)
Fiscal Year 2008: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2007: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2006: ¥8,400,000 (Direct Cost: ¥8,400,000)
Keywords脊索動物 / ホヤ / 発生 / 遺伝子 / 転写調節 / Hox / カタユウレイボヤ / 尾索動物 / Pitx / Otx / 発生遺伝子 / 機能 / 中枢神経系 / 尾 / FGF
Research Abstract

本研究では、動物の系統を通じて構造がよく保存された鍵発生遺伝子のいくつかについて、ホヤの発生における機能、転写調節機構の解析を行った。その結果、ホヤのオタマジャクシ幼生をつくる過程には、脊椎動物と同様な発生遺伝子カスケードとともに、脊椎動物とは大きく異なる機能、発現の制御機構があることが分かった。これらの結果は、脊索動物の発生機構の特徴とは何か、どの程度の可塑性があるのかを明らかにする手掛かりとなる。

Report

(4 results)
  • 2008 Annual Research Report   Final Research Report ( PDF )
  • 2007 Annual Research Report
  • 2006 Annual Research Report
  • Research Products

    (38 results)

All 2009 2008 2007 2006 Other

All Journal Article (16 results) (of which Peer Reviewed: 14 results) Presentation (18 results) Book (1 results) Remarks (3 results)

  • [Journal Article] Evolutionary roots of blastoporal expression and 'organizer'activity of the vertebrate gastrula organizer gene lhx1 and its ancient metazoan paralog lhx32009

    • Author(s)
      Yasuoka, Y., Kobayashi, M., Kurokawa, D., Akasaka, K., Saiga, H. and Taira, M.
    • Journal Title

      Development (in press)

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Left-right asymmetric expression of Pitx is regulated by the asymmetric Nodal signaling through an intronic enhancer in Ciona intestinalis2008

    • Author(s)
      Yoshida, K. and Saiga, H
    • Journal Title

      Dev. Genes Evol 218

      Pages: 353-360

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Comprehensive survey and classification of homeobox genes in the genome of amphioxus2008

    • Author(s)
      Takatori, N., Butts, T., Candiani, S., Pestarino, M., Ferrier, D.E.K., Saiga, H. and Holland, P.W.H
    • Journal Title

      Branchiostoma floridae. Dev. Genes Evol 218

      Pages: 579-590

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] The amphioxus genome illuminates vertebrate origins and cephalochordate biology2008

    • Author(s)
      Holland, L.Z., Albalat, R., Azumi,K., Benito-Gutierrez, E., Blow, M.J., Bronner-Fraser, M., Brunet, F., Butts, T., Candiani, S., Dishaw, L.J., Ferrier, D.E.K., Garcia-Fernandez, J., Gibson-Brown, J.J., Gissi, C., Godzik, A., Hallbook, F., Hirose, D., Hosomichi, K., Ikuta, T., Inoko, H., Kasahara, M., Kasamatsu, J., Kawashima, T., Kimura, A., Kobayashi, M., Kozmik, Z., Kubokawa,K., Laudet,V., Litman, G.W., McHardy, A.C., Meulemans, D., Nonaka, M., Olinski, R.P., Pancer, Z., Pennacchio, L.A., Pestarino, M., Rast, J.P., Rigoutsos, I., Robinson-Rechavi, M., Roch, G., Saiga, H., Sasakura, Y., Satake, M., Satou, Y., Schubert, M., Sherwood, N., Shiina, T., Takatori, N., Tello, J., Vopalensky, P., Wada, S., Xu, A., Ye, Y., Yoshida, K., Yoshizaki, F., Jr-Kai, Y., Zhang, Q., Zmasek, C.M., Putnam, N.H., Rokhsar, D.S., Satoh, N. and Holland, P.W.H.
    • Journal Title

      Genome Res 18

      Pages: 1100-1111

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Evolution of CUT class homeobox genes in the lineage to the vertebrates : insights from the analysis of the genomic sequence of amphioxus2008

    • Author(s)
      Takatori, N. and Saiga, H
    • Journal Title

      Branchiostoma floridae. Int. J. Dev. Biol 52

      Pages: 969-977

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Left-right asymmetric expression of Pitx is regulated by the asymmetric Nodal signaling through anintronic enhancer in Ciona intestinalis.2008

    • Author(s)
      Yoshida, K., Saiga, H.
    • Journal Title

      Dev. Genes Evol. 218

      Pages: 353-360

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The amphioxus genome illuminates vertebrate origins and cephalochordate biology.2008

    • Author(s)
      Holland, L. Z., . . . ., Saiga, H., Putnam. N. H., Rokhsar, D. S., Satoh, N., Holland, P. W. H., 他56名
    • Journal Title

      Genome Research 18

      Pages: 1100-1111

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Evolution of CUT class homeobox genes : insights from the genome of the amphioxus, Branchiostoma floridae.2008

    • Author(s)
      Takatori, N., Saiga, H.
    • Journal Title

      Int. J. Dev. Biol. 52

      Pages: 969-977

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Comprehensive survey and classification of homeobox genes in the genome of amphioxus, Branchiostoma floridae.2008

    • Author(s)
      Takatori, N., Butts, T., Candiani, S., Pestarino, M., Ferrier, D. E. K., Saiga, H., Holland, P. W. H.
    • Journal Title

      Dev. Genes Evol. 218

      Pages: 579-590

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Regionalization of the tail-tip epidermis requires inductive influence from vegetal cells and FGF signaling in the development of an ascidian, Halocynthia roretzi.2008

    • Author(s)
      Takatori, N., Wada, S, and Saiga, H.
    • Journal Title

      Zoological Science 24

      Pages: 441-448

    • NAID

      40015410960

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Dynamic change in the expression of developmental genes in the ascidian central nervous system: revisit to the tripartite model and the origin of the midbrain-hindbrain boundary region.2008

    • Author(s)
      Ikuta, T. and Saiga, H.
    • Journal Title

      Developmental Biology 312

      Pages: 631-641

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Evolution of CUT class homeobox genes in the lineage to the vertebrates:insights from the analysis of the genomic sequence of amphioxus, Branchiostoma floridae,2008

    • Author(s)
      Takatori, N. and Saiga, H.
    • Journal Title

      Internationl Journal of Developmental Biology (印刷中)

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Dynamic change in the expression of developmental genes in the ascidian central nervous system : revisit to the tripartite model and the origin of the midbrain-hindbrain boundary region2007

    • Author(s)
      Ikuta, T. and Saiga, H
    • Journal Title

      Dev. Biol 312

      Pages: 631-643

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Regionalization of the tail-tip epidermis requires inductive influence from vegetal cells and FGF signaling in the development of an ascidian2007

    • Author(s)
      Takatori, N., Wada, S. and Saiga, H.
    • Journal Title

      Halocynthia roretzi. Zool. Sci 24

      Pages: 441-448

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Regionalization of the tail-tip epidermis requires inductive influence from vegetal cells and FGF signaling in the development of an ascidian, Halocynthia roretzi.2007

    • Author(s)
      Takatori, N., Wada, S., Saiga, H.
    • Journal Title

      Zoological Science (印刷中)

    • NAID

      40015410960

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Hox遺伝子に進化2006

    • Author(s)
      生田哲郎, 西駕秀俊
    • Journal Title

      生体の科学 57

      Pages: 504-505

    • Related Report
      2006 Annual Research Report
  • [Presentation] Making very similar embryos with divergent genomes : conservation of transcriptional regulatory mechanisms of Otx between the ascidians2008

    • Author(s)
      Saiga, H.
    • Organizer
      Halocynthia roretzi and Ciona intestinalis
    • Place of Presentation
      神戸
    • Related Report
      2008 Final Research Report
  • [Presentation] Left-right asymmetric expression of Pitx is regulated by the asymmetric Nodal signaling through an intronic enhancer in Ciona intestinalis2008

    • Author(s)
      Yoshida, K. and Saiga, H.
    • Organizer
      Frontiers in Developmental Biology : Joint Meeting of the French and Japanese Societies for Developmental Biology
    • Place of Presentation
      Gien, France
    • Related Report
      2008 Final Research Report
  • [Presentation] カタユウレイボヤHox遺伝子の機能解析2008

    • Author(s)
      生田哲朗, 濱田麻友子, 佐藤ゆたか, 佐藤矩行, 西駕秀俊
    • Organizer
      日本動物学会第79回大会
    • Place of Presentation
      福岡
    • Related Report
      2008 Final Research Report
  • [Presentation] 二種のギボシムシにおけるHox 遺伝子およびParaHox 遺伝子の単離と後生動物の進化2008

    • Author(s)
      生田哲朗, 宮本教生, 田川訓史, トムハンフリー, 斎藤康典, 佐藤矩行, 西駕秀俊
    • Organizer
      日本動物学会第79回大会
    • Place of Presentation
      福岡
    • Related Report
      2008 Final Research Report
  • [Presentation] Protein evolution of the LIM-homeodomain transcription factors Lhx1 and Lhx3 in acquisition of the vertebrate gastrula organizer2008

    • Author(s)
      Yasuoka,Y., Kobayashi, M., Kurokawa, D., Akasaka,K., Saiga,H. and Taira, M
    • Organizer
      Japanese Society of Developmental Biologists
    • Place of Presentation
      徳島
    • Related Report
      2008 Final Research Report
  • [Presentation] Protein evolution of the LIM-homeodomain transcription factors Lhx1 and Lhx3 in acquisition of the vertebrate gastrula organizer.2008

    • Author(s)
      Yasuoka, Y., Kobayashi, M., Kurokawa, D., Akasaka, K., Saiga, H., Taira, M.
    • Organizer
      41st Annual Meeting for the Japanese Society of Developmental Biologists
    • Place of Presentation
      徳島市
    • Related Report
      2008 Annual Research Report
  • [Presentation] カタユウレイボヤHox遺伝子の機能解析.2008

    • Author(s)
      生田哲朗, 濱田麻友子, 佐藤ゆたか, 佐藤矩行, 西駕秀俊
    • Organizer
      日本動物学会第79回大会
    • Place of Presentation
      福岡市
    • Related Report
      2008 Annual Research Report
  • [Presentation] 二種のギボシムシにおけるHox遺伝子およびParaHox遺伝子の単離と後生動物の進化.2008

    • Author(s)
      生田哲朗, 宮本教生, 田川訓史, トムハンフリー, 斎藤康典, 佐藤矩行, 西駕秀俊
    • Organizer
      日本動物学会第79回大会
    • Place of Presentation
      福岡市
    • Related Report
      2008 Annual Research Report
  • [Presentation] Left-right asymmetric expression of Pitx is regulated by the asymmetric Nodal signaling through an intronic enhancer in Ciona intestinalis.2008

    • Author(s)
      Yoshida, K., Saiga, H.
    • Organizer
      Frontiers in Developmental Biology : Joint Meeting of the French and Japanese Societies for Developmental Biology
    • Place of Presentation
      Gien, France
    • Related Report
      2008 Annual Research Report
  • [Presentation] Making very similar embryos with divergent genomes : conservation of transcriptional regulatory mechanisms of Otx between the ascidians Halocynthia roretzi and Ciona intestinalis.2008

    • Author(s)
      Saiga, H.
    • Organizer
      The 16th CDB Meeting “Cis-Sequence Regulation and its Evolution"
    • Place of Presentation
      神戸市
    • Related Report
      2008 Annual Research Report
  • [Presentation] Dynamic change in the expression of developmental genes in the central nervous system of Ciona intestinalis ; reconsideration of the tripartite model and the origin of the midbrain2007

    • Author(s)
      Ikuta, T. and Saiga, H.
    • Organizer
      First Pan American Congress in Developmental Biology
    • Place of Presentation
      Cancun, Mexico
    • Related Report
      2008 Final Research Report
  • [Presentation] カタユウレイボヤ中枢神経系における主要発生遺伝子のダイナミックな発現2007

    • Author(s)
      生田哲朗, 西駕秀俊
    • Organizer
      第40回日本発生生物学会・第59回日本細胞生物学会大会合同大会
    • Place of Presentation
      福岡
    • Related Report
      2008 Final Research Report 2007 Annual Research Report
  • [Presentation] LIMホメオドメインタンパク質Lim1とLim3のオーガナイザー活性における機能比較を通じた進化生物学的解析2007

    • Author(s)
      安岡有理, 小林正明, 黒川大輔, 赤坂甲治, 西駕秀俊, 平良眞規
    • Organizer
      第30回日本分子生物学会年会・第80回日本生化学会大会合同大会
    • Place of Presentation
      横浜
    • Related Report
      2008 Final Research Report
  • [Presentation] 下等脊索動物ゲノム配列の解析から見た脊索動物Cut クラスホメオボックス遺伝子の進化2007

    • Author(s)
      高鳥直士, 小林正明, 生田哲朗, 吉田慶太, 広瀬弾, 西駕秀俊
    • Organizer
      日本動物学会第78回大会
    • Place of Presentation
      弘前
    • Related Report
      2008 Final Research Report
  • [Presentation] ホヤの中枢神経系におけるOtx遺伝子の転写調節機構の解析2007

    • Author(s)
      広瀬弾, 西駕秀俊
    • Organizer
      日本動物学会第78回大会
    • Place of Presentation
      弘前
    • Related Report
      2008 Final Research Report
  • [Presentation] カタユウレイボヤの発生における左右非対称な遺伝子発現とその調節機構の解析2007

    • Author(s)
      吉田慶太, 西駕秀俊
    • Organizer
      日本動物学会第78回大会
    • Place of Presentation
      弘前
    • Related Report
      2008 Final Research Report
  • [Presentation] カタユウレイボヤの消化管形成と遺伝子発現2007

    • Author(s)
      守山裕大, 生田哲朗, 小笠原道生, 西駕秀俊
    • Organizer
      日本動物学会関東支部第59回大会
    • Place of Presentation
      東京
    • Related Report
      2008 Final Research Report
  • [Presentation] カタユレイボヤのHox遺伝子の機能解析2006

    • Author(s)
      生田哲朗, 佐藤矩行, 西駕秀俊
    • Organizer
      日本発生生物学会第39回大会
    • Place of Presentation
      広島
    • Related Report
      2008 Final Research Report
  • [Book] 背側神経管の出現. 第5章神経系の多様性 その進化. シリーズ21世紀動物科学第7巻(阿形清和, 小泉修共編)2007

    • Author(s)
      西田宏記, 西駕秀俊
    • Publisher
      培風館
    • Related Report
      2008 Final Research Report
  • [Remarks] 生田哲朗, 西駕秀俊(2006)Hox 遺伝子の進化. 生体の科学57,504-505.

    • Related Report
      2008 Final Research Report
  • [Remarks]

    • URL

      http://dept.biol.metro-u.ac.jp/labo.asp?ID=devpro

    • Related Report
      2008 Annual Research Report
  • [Remarks]

    • URL

      http://dept.biol.metro-u.ac.jp/labo.asp?ID=devpro

    • Related Report
      2007 Annual Research Report

URL: 

Published: 2006-04-01   Modified: 2016-04-21  

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