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Transcription regulatory networks underlying specification of neuronal types in the nervous system of the ascidian

Research Project

Project/Area Number 18370089
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Developmental biology
Research InstitutionUniversity of Hyogo

Principal Investigator

KUSAKABE Takehiro  University of Hyogo, 大学院・生命理学研究科, 准教授 (40280862)

Project Period (FY) 2006 – 2008
Project Status Completed (Fiscal Year 2008)
Budget Amount *help
¥18,320,000 (Direct Cost: ¥15,200,000、Indirect Cost: ¥3,120,000)
Fiscal Year 2008: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
Fiscal Year 2007: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
Fiscal Year 2006: ¥4,800,000 (Direct Cost: ¥4,800,000)
Keywordsゲノム / 遺伝子 / 発生・分化 / 発現制御 / 脳・神経
Research Abstract

体のつくりや遺伝子構成が単純な海産動物ホヤをモデルとして、神経細胞の個性がつくられる仕組みを探った。グルタミン酸、GABAなど主要な神経伝達物質を使う神経細胞について、各細胞に固有の性質を与える遺伝子が特異的にはたらく仕組みの一端を解明した。ゲノム情報を生物間で比較することによって、遺伝情報の読み取りを調節するDNA配列(シス調節配列)とそこに結合するタンパク質(転写調節因子)を同定した。また、様々な神経細胞ではたらくシス調節配列を解析し、結果をデータベースとして公開した。

Report

(4 results)
  • 2008 Annual Research Report   Final Research Report ( PDF )
  • 2007 Annual Research Report
  • 2006 Annual Research Report
  • Research Products

    (62 results)

All 2010 2009 2008 2007 Other

All Journal Article (18 results) (of which Peer Reviewed: 7 results) Presentation (38 results) Book (4 results) Remarks (2 results)

  • [Journal Article] Simple motor system of the ascidian larva : neuronal complex comprising putative cholinergic and GABAergic/glycinergic neurons2010

    • Author(s)
      Takeo Horie, Masashi Nakagawa, Yasunori Sasakura, Takehiro G. Kusakabe, Motoyuki Tsuda
    • Journal Title

      Zool.Sci. 27(2)

      Pages: 181-190

    • Related Report
      2008 Final Research Report
  • [Journal Article] Distinctive expression patterns of Hedgehog pathway genes in the Ciona intestinalis larva : implications for a role of Hedgehog signaling in postembryonic development and chordate evolution2010

    • Author(s)
      Tariq A. F. M. Tariqul Islam, Pricila Khan Moly, Yuki Miyamoto, Takehiro G. Kusakabe
    • Journal Title

      Zool.Sci. 27(2)

      Pages: 84-90

    • Related Report
      2008 Final Research Report
  • [Journal Article] Evolution and the origin of the visual retinoid cycle in vertebrates2009

    • Author(s)
      Takehiro G. Kusakabe, Noriko Takimoto, Minghao Jin, Motoyuki Tsuda
    • Journal Title

      Phil.Trans.R.Soc.Lond. 364(1531)

      Pages: 2897-2910

    • Related Report
      2008 Final Research Report
  • [Journal Article] Tube formation by complex cellular processes in Ciona intestinalis notochord2009

    • Author(s)
      Bo Dong, Takeo Horie, Elsa Denker, Takehiro G. Kusakabe, Motoyuki Tsuda, William C. Smith, Di Jiang
    • Journal Title

      Dev.Biol. 330(2)

      Pages: 237-249

    • Related Report
      2008 Final Research Report
  • [Journal Article] Cell type and function of neurons in the ascidian nervous system2009

    • Author(s)
      Takeo Horie, Yasunori Sasakura, Masashi Nakagawa, Takehiro G. Kusakabe
    • Journal Title

      Dev.Growth & Differ 51(3)

      Pages: 207-220

    • Related Report
      2008 Final Research Report
  • [Journal Article] Pigmented and nonpigmented ocelli in the brain vesicle of the ascidian larva.2009

    • Author(s)
      Takeo Horie, Daisuke Sakurai, Hisashi Ohtsuki, Akihisa Terakita, Yoshinori Shichida, Jiro Usukura, Takehiro Kusakabe, Motoyuki Tsuda
    • Journal Title

      J.Comp.Neurol. 509(1)

      Pages: 88-102

    • Related Report
      2008 Final Research Report
  • [Journal Article] Cell type and function of neurons in the ascidian nervoussystem2009

    • Author(s)
      Takeo Horie
    • Journal Title

      Development, Growth & Differentiation 51

      Pages: 207-220

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Tube formation by complex cellular processes in Ciona intestinalis notochord2009

    • Author(s)
      Bo Dong
    • Journal Title

      Developmental Biology (印刷中)

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Markov chain-based promoter structure modeling for tissue-specific expression pattern prediction2008

    • Author(s)
      Alexis Vandenbon, Yuki Miyamoto, Noriko Takimoto, Takehiro Kusakabe, Kenta Nakai
    • Journal Title

      DNA Res. 15(1)

      Pages: 3-11

    • Related Report
      2008 Final Research Report
  • [Journal Article] Glutamatergic networks in the Ciona intestinalis larva2008

    • Author(s)
      Takeo Horie, Takehiro Kusakabe, Motoyuki Tsuda
    • Journal Title

      J.Comp.Neurol. 508(2)

      Pages: 249-263

    • Related Report
      2008 Final Research Report
  • [Journal Article] Pigmented and nonpigmented ocelli in the brain vesicle of the ascidian larva2008

    • Author(s)
      Takeo Horie
    • Journal Title

      Journal of Comparative Neurology 509

      Pages: 88-102

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Glutamatergic networks in the Ciona intestinalis larva2008

    • Author(s)
      Takeo Horie
    • Journal Title

      Journal of Comparative Neurology 508

      Pages: 249-263

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Markov chain-based promoter struture modeling for tissue-specific expression pattern prediction2008

    • Author(s)
      Alexis Vandenbon
    • Journal Title

      DNA Research 15

      Pages: 3-11

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Glutamatergic networks in the Ciona intestinalis larva2008

    • Author(s)
      Takeo Horie
    • Journal Title

      Journal of Comparative Neurology 509

      Pages: 88-102

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Comparative genomics identifies a cis-regulatory module that activates transcription in specific subsets of neurons in2007

    • Author(s)
      Reiko Yoshida, Takeo Horie, Motoyuki Tsuda, Takehiro G. Kusakabe
    • Journal Title

      Ciona intestinalis larva.Dev.Growth Differ. 49(8)

      Pages: 657-667

    • Related Report
      2008 Final Research Report
  • [Journal Article] Photoreceptive systems in ascidians2007

    • Author(s)
      Takehiro Kusakabe, Motoyuki Tsuda
    • Journal Title

      Photochem.Photobiol. 83(2)

      Pages: 248-252

    • Related Report
      2008 Final Research Report
  • [Journal Article] Comparative genomics identifies a cis-regulatory module that activates transcription in specific subsets of neurons in Ciona intestinalis larva2007

    • Author(s)
      Reiko Yoshida
    • Journal Title

      Development, Growth & Differentiation 49

      Pages: 657-667

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] photoreceptive systems in ascidians2007

    • Author(s)
      Takehiro Kusakabe
    • Journal Title

      Photochemistry and Photobiology 83

      Pages: 248-252

    • Related Report
      2006 Annual Research Report
  • [Presentation] Decoding cis-regulatory systems of cell type-specific genes in the tunicate genome2009

    • Author(s)
      Takehiro G. Kusakabe
    • Organizer
      International Symposium on Marine Genomics 2009
    • Place of Presentation
      Southern Plaza Kaiho, Okinawa
    • Year and Date
      2009-12-18
    • Related Report
      2008 Final Research Report
  • [Presentation] カタユウレイボヤ幼生のGABA/グリシン作動性ニューロンにおける遺伝子発現を制御するシス調節配列と転写調節因子の機能解析2009

    • Author(s)
      西辻光希
    • Organizer
      分子生物学会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2009-12-12
    • Related Report
      2008 Final Research Report
  • [Presentation] ホヤ幼生におけるグルタミン酸作動性ニューロン特異的な遺伝子発現調節機構とPOU転写遺伝子Ci-P0U-IVの役割2009

    • Author(s)
      大道裕
    • Organizer
      分子生物学会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2009-12-12
    • Related Report
      2008 Final Research Report
  • [Presentation] cis-Regulatory modules and transcription factors that control gene expression in glutamatergic and GABAergic/glycinergic neurons in the Ciona intestinalis larva2009

    • Author(s)
      Koki Nishitsuji
    • Organizer
      5th Tunicate Meeting
    • Place of Presentation
      Okinawa Industry Support Center
    • Year and Date
      2009-06-24
    • Related Report
      2008 Final Research Report
  • [Presentation] Dopaminergic cells in Ciona intestinalis larva share some features with vertebrate dopaminergic amacrine cells.2009

    • Author(s)
      Florian Razy-Krajka
    • Organizer
      5th Tunicate Meeting
    • Place of Presentation
      Okinawa Industry Support Center
    • Year and Date
      2009-06-24
    • Related Report
      2008 Final Research Report
  • [Presentation] Ptfl, a bHLH transcription factor, defines the dopaminergic neuronal cell fate in ascidian larva2009

    • Author(s)
      Takeo Horie
    • Organizer
      5th Tunicate Meeting
    • Place of Presentation
      Okinawa Industry Support Center
    • Year and Date
      2009-06-24
    • Related Report
      2008 Final Research Report
  • [Presentation] Distinct expression patterns of hedgehog signaling pathway genes during larval development of Ciona intestinalis2009

    • Author(s)
      A. F. M. Tariqul Islam
    • Organizer
      5th Tunicate Meeting
    • Place of Presentation
      Okinawa Industry Support Center
    • Year and Date
      2009-06-21
    • Related Report
      2008 Final Research Report
  • [Presentation] Neuronal differentiation in the central nervous system territories marked by the expression of Otx, Pax-2/5/8, and Engenes in the ascidian larva2009

    • Author(s)
      Koki Nishitsuji
    • Organizer
      日本発生生物学会第42回大会
    • Place of Presentation
      朱鷺メッセ 新潟コンべンシヨンセンター
    • Related Report
      2008 Final Research Report
  • [Presentation] DiI標識とレーザー細胞破壊によるカタユウレイボヤ幼生の光受容細胞の細胞系譜解析2008

    • Author(s)
      A. F. M. Tariqul Islam
    • Organizer
      日本動物学会第79回大会
    • Place of Presentation
      福岡大学七隈キャンパス
    • Year and Date
      2008-09-07
    • Related Report
      2008 Final Research Report
  • [Presentation] カタユウレイボヤ神経系特異的遺伝子の転写調節領域の大規模解析2008

    • Author(s)
      西辻光希
    • Organizer
      日本動物学会第79回大会
    • Place of Presentation
      福岡大学七隈キャンパス
    • Year and Date
      2008-09-07
    • Related Report
      2008 Annual Research Report 2008 Final Research Report
  • [Presentation] ホヤ幼生の神経発生におけるOtxとPax2/5/8の役割2008

    • Author(s)
      日下部岳広
    • Organizer
      第31回日本神経科学大会
    • Place of Presentation
      東京国際フォーラム
    • Year and Date
      2008-09-07
    • Related Report
      2008 Annual Research Report 2008 Final Research Report
  • [Presentation] Cell lineage analysis of photoreceptor cells by laser ablation and DiI labeling in the Ciona intestinalis larva2008

    • Author(s)
      A. F. M. Tariqul Islam
    • Organizer
      日本動物学会第79回大会
    • Place of Presentation
      福岡大学七隈キャンパス
    • Year and Date
      2008-09-07
    • Related Report
      2008 Annual Research Report
  • [Presentation] ホヤにおける視覚サイクル機構2008

    • Author(s)
      瀧本 紀子
    • Organizer
      日本動物学会第79回大会
    • Place of Presentation
      福岡大学七隈キャンパス
    • Year and Date
      2008-09-07
    • Related Report
      2008 Annual Research Report
  • [Presentation] Identification and characterization of a cis-regulatory module required for specific gene expression in a subset of GABA/glycinergic neurons of the Ciona intestinalis larva2008

    • Author(s)
      Koki Nishitsuji
    • Organizer
      日本発生生物学会第41回大会
    • Place of Presentation
      徳島県郷土文化会館
    • Related Report
      2008 Annual Research Report 2008 Final Research Report
  • [Presentation] Ontogeny of glutamatergic, GABA/glycinergic, and cholinergic neurons in the Ciona intestinalis larva2008

    • Author(s)
      Yuki Morimoto
    • Organizer
      日本発生生物学会第41回大会
    • Place of Presentation
      徳島県郷土文化会館
    • Related Report
      2008 Final Research Report
  • [Presentation] Ci-POU-IV, a POU domain transcription factor, is required for development of epidermal glutamatergic neurons in the Ciona intestinalis larva2008

    • Author(s)
      Takako Suzuki
    • Organizer
      日本発生生物学会第41回大会
    • Place of Presentation
      徳島県郷土文化会館
    • Related Report
      2008 Final Research Report
  • [Presentation] Roles of hedgehog signaling in Ciona intestinalis development : insights from hedgehog and Gli expression and cyclopamine treatment2008

    • Author(s)
      A. F. M. Tariqul Islam
    • Organizer
      日本発生生物学会第41回大会
    • Place of Presentation
      徳島県郷土文化会館
    • Related Report
      2008 Final Research Report
  • [Presentation] Identification and characterization of a cis-regulatory module required for specific gene expression in a subset of GABA/glycinergic neurons of the Ciona intestinalis larva.2008

    • Author(s)
      Koki Nishitsuji
    • Organizer
      Joint meeting of the Societe Franqaise de Biologic du Development / Japanese Society of Developmental Biologists
    • Place of Presentation
      日 GIENS (FRANCE)
    • Related Report
      2008 Final Research Report
  • [Presentation] Gene regulatory mechanisms underlying the determination of neuronal identity in the ascidian larva.2008

    • Author(s)
      Takehiro Kusakabe
    • Organizer
      The 16th CDB Meeting : Cis-sequence Regulation and its Evolution
    • Place of Presentation
      理研 CDB (神戸)
    • Related Report
      2008 Final Research Report
  • [Presentation] Roles of hedgehog signaling in Ciona intestinalis development : insights from hedgehog and Gli expression and cyclopamine treatment2008

    • Author(s)
      A. F. M. Tariqul Islam
    • Organizer
      日本発生生物学会第4 1回大会
    • Place of Presentation
      徳島県郷土文化会館
    • Related Report
      2008 Annual Research Report
  • [Presentation] Ontogeny of glutamatergic, GABA/glycinergic, and cholinergicneurons in the Ciona intestinalis larva2008

    • Author(s)
      森本 熊紀
    • Organizer
      日本発生生物学会第41回大会
    • Place of Presentation
      徳島県郷土文化会館
    • Related Report
      2008 Annual Research Report
  • [Presentation] Ci-POU-IV, a POU domain transcription factor, is required for development of epidermal glutamatergic neurons in the Cionaintestinalis larva2008

    • Author(s)
      鈴木 貴子
    • Organizer
      日本発生生物学会第41回大会
    • Place of Presentation
      徳島県郷土文化会館
    • Related Report
      2008 Annual Research Report
  • [Presentation] Identification and characterization of a cis-regulatory modulerequired for specific gene expression in a subset of GABA/glycinergic neurons of the Ciona intestinalis larva2008

    • Author(s)
      Koki Nishitsuji
    • Organizer
      Joint meeting of the Societe Francaisede Biologie du Development / Japanese Society of Developmental Biologists
    • Place of Presentation
      GIENS (FRANCE)
    • Related Report
      2008 Annual Research Report
  • [Presentation] Gene Regulatory Mechanisms underlying the Determination of Neuronal Identity in the Ascidian Larva2008

    • Author(s)
      Takehiro Kusakabe
    • Organizer
      The 16th CDB Meeting : Cis-sequence Regulation and its Evolution
    • Place of Presentation
      理研CDB(神戸)
    • Related Report
      2008 Annual Research Report
  • [Presentation] Molecular and cellular mechanisms of glutamatergic and GABAergic neuron development in the ascidian larva2007

    • Author(s)
      Takehiro G. Kusakabe
    • Organizer
      The Sixth Okazaki Biology Conference "Marine Biology"
    • Place of Presentation
      Okazaki Conference Center
    • Year and Date
      2007-12-05
    • Related Report
      2008 Final Research Report
  • [Presentation] Molecular and celluar mechanisms of glutamatergic and GAB Aergicneuron development in the ascidian larva2007

    • Author(s)
      Takehiro G.Kusakabe
    • Organizer
      The SiXth Okazaki Biology Conference
    • Place of Presentation
      岡崎コンファレンスセンター
    • Year and Date
      2007-12-05
    • Related Report
      2007 Annual Research Report
  • [Presentation] ホヤの光受容システムから探る脊椎動物の頭部感覚器官の起源2007

    • Author(s)
      日下部 岳広
    • Organizer
      日本進化学会第9回京都大会
    • Place of Presentation
      京都大学 吉田キャンパス
    • Year and Date
      2007-08-31
    • Related Report
      2007 Annual Research Report
  • [Presentation] 比較ゲノムによるカタユウレイボヤ GABA 作動性ニューロンおよびグルタミン酸作動性ニューロンのシス調節領域の同定2007

    • Author(s)
      西辻光希
    • Organizer
      第40回日本発生生物学会・第59回日本細胞生物学会 合同大会
    • Place of Presentation
      福岡国際会議場
    • Year and Date
      2007-05-30
    • Related Report
      2008 Final Research Report
  • [Presentation] 比較ゲノムによるカタユウレイボヤGABA作動性ニューロンおよびグルタミン作動性ニューロンのシス調節領域の同定2007

    • Author(s)
      西辻 光希
    • Organizer
      第40回日本発生生物学会・第59回日本細胞生物学会合同大会
    • Place of Presentation
      福岡国際会議場
    • Year and Date
      2007-05-30
    • Related Report
      2007 Annual Research Report
  • [Presentation] カタユウレイボヤ胚発生および変態過程における光受容器官の形成機構と脊椎動物の眼および松果体の起源2007

    • Author(s)
      笹倉 美幸
    • Organizer
      第40回日本発生生物学会・第59回日本細胞生物学会合同大会
    • Place of Presentation
      福岡国際会議場
    • Year and Date
      2007-05-30
    • Related Report
      2007 Annual Research Report
  • [Presentation] 転写制御領域に見られる共通構造のコンピューターモデリングに向けて2007

    • Author(s)
      中井謙太
    • Organizer
      第40回日本発生生物学会・第59回日本細胞生物学会 合同大会 ミニシンポジウム「形を作る遺伝子調節ネットワーク」
    • Place of Presentation
      福岡国際会議場
    • Year and Date
      2007-05-28
    • Related Report
      2008 Final Research Report
  • [Presentation] 転写制御領域に見られる共通構造のコンピューターモデリングに向けて2007

    • Author(s)
      中井 謙太
    • Organizer
      第40回日本発生生物学会・第59回日本細胞生物学会合同大会
    • Place of Presentation
      福岡国際会議場
    • Year and Date
      2007-05-28
    • Related Report
      2007 Annual Research Report
  • [Presentation] カタユウレイボヤ幼生のコリン作動性およびGABA作動性神経回路の可視化2007

    • Author(s)
      森元熊紀
    • Organizer
      2007年度日本動物学会近畿支部研究発表会
    • Place of Presentation
      神戸山手大学
    • Year and Date
      2007-05-19
    • Related Report
      2008 Final Research Report
  • [Presentation] 比較ゲノムによるニューロン特異的遺伝子のシス調節モジュールの同定2007

    • Author(s)
      西辻光希
    • Organizer
      2007年度日本動物学会近畿支部研究発表会
    • Place of Presentation
      神戸山手大学
    • Year and Date
      2007-05-19
    • Related Report
      2008 Final Research Report
  • [Presentation] Role of hedgehog signaling in neuronal patterning of the Ciona intestinalis larva2007

    • Author(s)
      A. E M. Tariqul Islam
    • Organizer
      2007年度日本動物学会近畿支部研究発表会
    • Place of Presentation
      神戸山手大学
    • Year and Date
      2007-05-19
    • Related Report
      2008 Final Research Report
  • [Presentation] GFP/WGA二重標識によるホヤ幼生のコリン作動性およびGABA作動性神経回路の可視化2007

    • Author(s)
      森元熊紀
    • Organizer
      第2回ホヤ研究集会
    • Place of Presentation
      下田
    • Year and Date
      2007-05-08
    • Related Report
      2008 Final Research Report
  • [Presentation] Markov chain-based promoter structure modeling2007

    • Author(s)
      Alexis Vandenbon
    • Organizer
      4th International Tunicate Meeting
    • Place of Presentation
      Villefranche-sur-Mer.
    • Related Report
      2008 Final Research Report
  • [Presentation] Markov chain-based promoter structure modeling.2007

    • Author(s)
      Alexis Vandenbon
    • Organizer
      15th Annual International Conference on Intelligent Systems for Molecular Biology (ISMB) & 6th European Conference on Computational Biology (ECCB)
    • Place of Presentation
      Vienna, Austria
    • Related Report
      2008 Final Research Report
  • [Book] 『動物の多様な生き方5さまざまな神経系をもつ動物たち : 神経系の比較生物学』(比較生理生化学会編)2009

    • Author(s)
      日下部岳広 (共著)
    • Publisher
      共立出版
    • Related Report
      2008 Final Research Report
  • [Book] 『身近な動物を使った実験1-ホヤ メダカ・ゼブラフィッシュ キンギョカエル』(鈴木範男編)2009

    • Author(s)
      日下部岳広 (共著)
    • Publisher
      三共出版
    • Related Report
      2008 Final Research Report
  • [Book] 身近な動物を使った実験1 ホヤ2009

    • Author(s)
      日下部 岳広
    • Publisher
      三共出版
    • Related Report
      2008 Annual Research Report
  • [Book] 動物はなぜ多様な神経系をもっか? : 神経系の比較生物学第10章 ホヤの神経系と行動2009

    • Author(s)
      日下部 岳広
    • Publisher
      共立出版(印刷中)
    • Related Report
      2008 Annual Research Report
  • [Remarks] ホヤ遺伝子制御データベース (DBTGR)

    • URL

      http://dbtgr.hgc.jp/

    • Related Report
      2008 Final Research Report
  • [Remarks] ホヤ統合データベース (Aniseed)

    • URL

      http://crfb.univ-mrs.fr/aniseed/

    • Related Report
      2008 Final Research Report

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Published: 2006-04-01   Modified: 2016-04-21  

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