• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Studies on molecular mechanisms of mammalian early embryonic development by means of recessive lethal mutations.

Research Project

Project/Area Number 18370094
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Developmental biology
Research InstitutionResearch Institute, Osaka Medical Center for Maternal and Child Health

Principal Investigator

MATSUO Isao  Research Institute, Osaka Medical Center for Maternal and Child Health, 大阪府立母子保健総合医療センター(研究所) (10264285)

Project Period (FY) 2006 – 2009
Project Status Completed (Fiscal Year 2009)
Budget Amount *help
¥17,860,000 (Direct Cost: ¥15,400,000、Indirect Cost: ¥2,460,000)
Fiscal Year 2009: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2008: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2007: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2006: ¥7,200,000 (Direct Cost: ¥7,200,000)
Keywordsマウス / 劣性致死変異体 / ヘパラン硫酸鎖 / 発生 / プロテオグリカン / FGF / 遺伝学 / Ext2 / 胚体外外胚葉 / 劣性致死変異 / へパラン硫酸鎖
Research Abstract

We identified the transgene insertion allele of mouse Ext2, one of the causative genes of human multiple hereditaryexostoses, which catalyzes elongation of HS-chains. Marker expression analyses of Ext2-deficient embryos revealed that HS-chains are essential for response to Fibroblast growth factor (FGF) signaling and, in particular, local distribution of the FGF ligands as well as FGF signaling. Moreover, HS-chains expression attached with cell surface is distinctively specific to those areas in which FGF signaling is potentially active. Fur

Report

(6 results)
  • 2009 Annual Research Report   Final Research Report ( PDF )
  • 2008 Annual Research Report   Self-evaluation Report ( PDF )
  • 2007 Annual Research Report
  • 2006 Annual Research Report
  • Research Products

    (32 results)

All 2010 2009 2008 2007 Other

All Journal Article (19 results) (of which Peer Reviewed: 18 results) Presentation (7 results) Book (1 results) Remarks (5 results)

  • [Journal Article] FGF signaling directs a center-to-pole expansion of tubulogenesis in mouse testis differentiation2010

    • Author(s)
      Ryuji Hiramatsu, Kyoko Harikae, Naoki Tsunekawa, Masamichi Kurohmaru, Isao Matsuo, Yoshiakira Kanai
    • Journal Title

      Development 137

      Pages: 303-312

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] FGF signaling directs a center-to-pole expansion of tubulogenesis in mousetestis differentiation.2010

    • Author(s)
      Ryuji Hiramatsu, et al.
    • Journal Title

      Development 137

      Pages: 303-312

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Dosage-dependent hedgehog signals integrated with Wnt/β-catenin signaling regulate external genitalia formation as an appendicular program2009

    • Author(s)
      Shinichi Miyagawa, Anne Moon, Ryuma Haraguchi, Chie Inoue, Masayo Harada, Chiaki Nakahara, Kentaro Suzuki, Daisuke Matsumaru, Takehito Kaneko, Isao Matsuo, Lei Yang, Makoto M. Taketo, Taisen Iguchi, Sylvia M. Evans, Gen Yamada
    • Journal Title

      Development 136

      Pages: 3969-3978

    • NAID

      120002469485

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Dosage-dependent hedgehog signals integrated with Wnt/β-catenin signaling regulate external genitalia formation as an appendicular program.2009

    • Author(s)
      Shinichi Miyagawa, et al.
    • Journal Title

      Development 136

      Pages: 3969-3978

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Alain Prochiantz and Takao K Hensch Experience-dependent transfer of Otx2 homeoprotein into the visual cortex activates postnatal plasticity2008

    • Author(s)
      Sayaka Sugiyama, Ariel A Di Nardo, Shinichi Aizawa, Isao Matsuo, Michel Volovitch
    • Journal Title

      Cell 134

      Pages: 508-520

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Possible involvement of SINEs in mammalian-specific brain formation2008

    • Author(s)
      Takeshi Sasaki, Hidenori Nishihara, Mika Hirakawa, Koji Fujimura, Mikiko Tanaka, Nobuhiro Kokubo, Chiharu Kimura-Yoshida, Isao Matsuo, Kenta Sumiyama, Naruya Saitou, Tomomi Shimogori, Norihiro Okada
    • Journal Title

      Proc. Natl. Acad. Sci. USA 105

      Pages: 4220-4225

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Experience-dependent transfer of Otx2homeoprotein into the visual cortex activatespostnatal plasticity2008

    • Author(s)
      Sayaka Sugiyama, Ariel A Di Nardo, Shinichi Aizawa, Isao Matsuo, Michel Volovitch, Alain Prochiantz and Takao K Hensch
    • Journal Title

      Cell 134

      Pages: 508-520

    • Related Report
      2008 Self-evaluation Report
    • Peer Reviewed
  • [Journal Article] Possible involvement of SINEs inmammalian-specific brain formation2008

    • Author(s)
      Takeshi Sasaki, Hidenori Nishihara, Mika Hirakawa, Koji Fujimura, Mikiko Tanaka, Nobuhiro Kokubo, Chiharu Kimura-Yoshida, Isao Matsuo , Kenta Sumiyama, Naruya Saitou, Tomomi Shimogori and Norihiro Okada
    • Journal Title

      Proc. Natl. Acad. Sci. USA 105

      Pages: 4220-4225

    • Related Report
      2008 Self-evaluation Report
    • Peer Reviewed
  • [Journal Article] Experience-dependent transfer of Otx2 homeoprotein into the visual cortex activates postnatal plasticity2008

    • Author(s)
      Sayaka Sugiyama, et al.
    • Journal Title

      Cell 134

      Pages: 508-520

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Possible involvement of SINEs in mammalian-specific brain formation.2008

    • Author(s)
      Takeshi Sasaki, et. al.
    • Journal Title

      Proc. Natl. Acad. Sci. USA 105

      Pages: 4220-4225

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Functional roles of Otx2 transcription factor in postnatal mouse retinal development2007

    • Author(s)
      Chieko Koike, Akihiro Nishida, Shinji Ueno, Hiromitsu Saito, Rikako Sanuki, Shigeru Sato, Akiko Furukawa, Shinichi Aizawa, Isao Matsuo, Noboru Suzuki, Mineo Kondo, Takahisa Furukawa
    • Journal Title

      Mol. Cell. Biol 27

      Pages: 8318-8329

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Dkk3-Cre BAC transgenic mouse line: A tool for highly-efficient deletion in retinal progenitor cells2007

    • Author(s)
      Shigeru Sato, Tatsuya Inoue, Koji Terada, Isao Matsuo, Shinichi Aizawa, Yasuo Tano, Takashi Fujikado, Takahisa Furukawa
    • Journal Title

      Genesis 45

      Pages: 502-507

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Crucial roles of Foxa2 in mouse anterior-posterior axis polarization via regulation of anterior visceral endoderm-specific genes2007

    • Author(s)
      Chiharu Kimura-Yoshida, E Tian, Hiroshi Nakano, Saori Amazaki, Kayo Shimokawa, Janet Rossant, Shinichi Aizawa, Isao Matsuo
    • Journal Title

      Proc. Natl. Acad. Sci. USA 104

      Pages: 5919-5924

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Functional roles of Otx2 transcription factor inpostnatal mouse retinal development2007

    • Author(s)
      Chieko Koike, Akihiro Nishida, Shinji Ueno, Hiromitsu Saito, Rikako Sanuki, Shigeru Sato, Akiko Furukawa, Shinichi Aizawa, Isao Matsuo ,Noboru Suzuki, Mineo Kondo, and Takahisa Furukawa
    • Journal Title

      Mol. Cell. Biol 27

      Pages: 8318-8329

    • Related Report
      2008 Self-evaluation Report
    • Peer Reviewed
  • [Journal Article] Dkk3-Cre BAC transgenic mouse line : A tool forhighly-efficient deletion in retinal progenitorcells2007

    • Author(s)
      Shigeru Sato, Tatsuya Inoue, Koji Terada, Isao Matsuo, Shinichi Aizawa, Yasuo Tano, Takashi Fujikado and Takahisa Furukawa
    • Journal Title

      Genesis 45

      Pages: 502-507

    • Related Report
      2008 Self-evaluation Report
    • Peer Reviewed
  • [Journal Article] Crucial roles of Foxa2 in mouseanterior-posterior axis polarization viaregulation of anterior visceral endoderm-specificgenes2007

    • Author(s)
      Chiharu Kimura-Yoshida, E Tian, Hiroshi Nakano, Saori Amazaki, Kayo Shimokawa, Janet Rossant, Shinichi Aizawa and Isao Matsuo
    • Journal Title

      Proc. Natl. Acad. Sci. USA 104

      Pages: 5919-5924

    • Related Report
      2008 Self-evaluation Report
    • Peer Reviewed
  • [Journal Article] Dkk3-Cre BAC transgenic mouse line : A tool for highly-efficient deletion in retinal progenitor cells.2007

    • Author(s)
      Shigeru Sato, et. al.
    • Journal Title

      Genesis 45

      Pages: 502-507

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Functional roles of Otx2 transcription factor in postnatal mouse retinal development2007

    • Author(s)
      Chieko Koike, et. al.
    • Journal Title

      Mol. Cell. Biol. 27

      Pages: 8318-8329

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Crucial roles of Foxa2 in mouse anterior-posterior axis polarization via regulation of anterior visceral endoderm-specific genes2007

    • Author(s)
      Chiharu Kimura-Yoshida et al.
    • Journal Title

      Proc.Natl.Acad.Sci.USA 104・13

      Pages: 5919-5924

    • Related Report
      2006 Annual Research Report
  • [Presentation] Spatio-temporal expression of heparan sulfate glycosaminoglycan chains modulates distribution of Fgf signaling during mammalian embrvogenesis2009

    • Author(s)
      Kayo Shimokawa, et al.
    • Organizer
      16th International Society of Developmental Biologists Congress
    • Place of Presentation
      Edinburgh, Scotland, UK
    • Year and Date
      2009-09-08
    • Related Report
      2009 Annual Research Report
  • [Presentation] Spatio-temporal expression of heparan sulfate glycosaminoglycan chains modulates distribution of Fgf signaling during mammalian embryogenesis2009

    • Author(s)
      Isao Matsuo
    • Organizer
      16th International Society of Developmental Biologists Congress
    • Place of Presentation
      UK. Edinburgh
    • Related Report
      2009 Final Research Report
  • [Presentation] Isao Matsuo Specific expression of heparan sulfate glycosaminoglycan chains is crucial for local distribution of FGF signaling in mouse extraembryonic ectoderm of the2009

    • Author(s)
      Isao Matsuo
    • Organizer
      第32回日本分子生物学会年会
    • Place of Presentation
      横浜
    • Related Report
      2009 Final Research Report
  • [Presentation] Heparan sulfate chains synthesized by Ext2 regulate Fgf signaling during mammalian embryogenesis2008

    • Author(s)
      Kazuhiro Mukai, et al.
    • Organizer
      Mouse Genetics & Genomics : Development and Disease
    • Place of Presentation
      CSH, New York, USA
    • Year and Date
      2008-11-01
    • Related Report
      2008 Annual Research Report
  • [Presentation] Heparan sulfate chains synthesized by Ext2 regulate Fgf signaling during mammalian embryogenesis2008

    • Author(s)
      Isao Matsuo
    • Organizer
      Mouse Genetics, Genomics: Development and Disease
    • Place of Presentation
      Cold Spring Harbor Laboratory, USA
    • Related Report
      2009 Final Research Report
  • [Presentation] Identification and characterization of a transgene insertion mutation of Ext2 gene encoding a glycosyltransferase.2007

    • Author(s)
      Kazuhiro Mukai, et. al.
    • Organizer
      Mouse Molecular Genetics Meeting
    • Place of Presentation
      Cambridge, UK
    • Year and Date
      2007-09-07
    • Related Report
      2007 Annual Research Report
  • [Presentation] Identification and characterization of a transgene insertion mutation of Ext2 gene encoding a glycosyltransferase2007

    • Author(s)
      Isao Matsuo
    • Organizer
      Mouse Molecular Genetics Meeting
    • Place of Presentation
      Wellcome Trust Genome Campus, Cambridge, UK
    • Related Report
      2009 Final Research Report
  • [Book] Wntシグナルとマウス前後軸決定~Wnt拮抗因子Dickkopf1遺伝子に着目して~医学のあゆみ2010

    • Author(s)
      吉田千春、平松竜司、松尾勲
    • Publisher
      (印刷中)
    • Related Report
      2009 Final Research Report
  • [Remarks]

    • URL

      http://www.med.osaka-u.ac.jp/pub/inst-mch/Byo/Byo.html

    • Related Report
      2009 Final Research Report
  • [Remarks]

    • URL

      http://www.med.osaka-u.ac.jp/pub/inst-mch/Byo/Byo.html

    • Related Report
      2009 Annual Research Report
  • [Remarks]

    • URL

      http://www.med.osaka-u.ac.jp/pub/inst-mch/Byo/Byo.html

    • Related Report
      2008 Self-evaluation Report
  • [Remarks]

    • URL

      http://www.med.osaka-u.ac.jp/pub/inst-mch/Byo/Byo.html

    • Related Report
      2008 Annual Research Report
  • [Remarks]

    • URL

      http://www.med.osaka-u.ac.jp/pub/inst-mch/Byo/Byo.html

    • Related Report
      2007 Annual Research Report

URL: 

Published: 2006-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi