Identification and functional analysis of genes related with microspore embryogenesis

• Project/Area Number: 18380003
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Breeding science
• Research Institution: Iwate University
• Principal Investigator: TAKAHATA Yoshihito Iwate University, 農学部, 教授 (10133894)
• Co-Investigator(Kenkyū-buntansha): NISHIO Takeshi 東北大学, 大学院・農学研究科, 教授 (30301039)
• Project Period (FY): 2006 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥16,420,000 (Direct Cost: ¥14,200,000、Indirect Cost: ¥2,220,000); Fiscal Year 2008: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2007: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2006: ¥6,800,000 (Direct Cost: ¥6,800,000)
• Keywords: Brassica / 小胞子培養 / 胚発生 / 遺伝子単離 / DNAマーカー / 機能解析

Research Abstract

アブラナ科作物の小胞子からの胚発生の機構を解析するために、胚発生に特異的な遺伝子の網羅的な単離を行い、さらにそれら遺伝子についてシロイヌナズナの相同遺伝子を利用した機能解析を行った。GASSHOと名付けた遺伝子は胚の表面組織の形成に関与し、EMKと名付けた遺伝子は胚形成に関与していることが推察された。また分子連鎖地図の構築を行い胚形成領域の予備的なマッピングを行った。

Research Products

• [Journal Article] Identification of genes specifically expressed in androgenesis-derived embryo in rapeseed (2008)
  • Author(s): Tsuwamoto R, Takahata Y
  • Journal Title: (Brassica napus L.)Breed Sci 58 Pages: 251-259
  • Peer Reviewed
• [Journal Article] GASSHO1 and GASSHO2 encoding a putative leucine-rich repeat transmembrane-type receptor kinase are essential for the normal development of the epidermal surface in Arabidopsis embryos (2008)
  • Author(s): Tsuwamoto R, Fukuoka H, Takahata Y
  • Journal Title: Plant J. 54 Pages: 30-42
  • Peer Reviewed
• [Journal Article] Identification of genes specifically expressed in androgenesis-derived embryo in raneseed (Brassica nanus L.) (2008)
  • Author(s): Tsuwamoto Ryo
  • Journal Title: Breeding Science 58 Pages: 251-259
  • Peer Reviewed
• [Journal Article] GASSHO1 and GASSHO2 encoding a putative leucine-rich repeat transmembrane-type receptor kinase are essential for the normal development of the epidermal surface in Arabidopsis embryos. (2008)
  • Author(s): Tsuwamoto Ryo
  • Journal Title: Plant Journal 54 Pages: 30-42
  • Peer Reviewed
• [Journal Article] Identification and characterization of genes expressed in early embryogenesis from microspores of Brassica napus (2007)
  • Author(s): Tsuwamoto R, Fukuoka H, Takahata Y
  • Journal Title: Planta 225 Pages: 641-652
  • Peer Reviewed
• [Journal Article] Identification and analysis of andorogenesis-specific expressed sequence tags in rapeseed using suppression subtractive hybridization. (2007)
  • Author(s): Tsuwamoto, R.
  • Journal Title: The 12th International Congress Abstract Pages: 131-131
• [Journal Article] セイヨウナタネ小胞子由来球状胚特異的ESTsの単離および発現解析 (2006)
  • Author(s): 津和本 亮
  • Journal Title: 育種学研究 8(別2) Pages: 338-338
• [Journal Article] The lack of Arabidopsis GASSHO1 and GASSHO2 genes encoding LRR-RLKs leads to the defect in cuticle during embryo stages. (2006)
  • Author(s): Tsuwamoto, R.
  • Journal Title: 15th Crucifer Genetic Workshop : Brassica 2006 Pages: 157-157
• [Presentation] P22a1 : sGFP導入ナタネを用いた小胞子胚発生初期発現遺伝子の単離 (2008)
  • Author(s): 酒井球絵・福岡浩之・横井修司・高畑義人
  • Organizer: 日本育種学会114回講演会
  • Place of Presentation: 彦根, 滋賀県立大学
  • Year and Date: 2008-10-12
• [Presentation] P22al : sGFP導入ナタネを用いた小胞子胚発生初期発現遺伝子の単離 (2008)
  • Author(s): 酒井球絵
  • Organizer: 日本育種学会114回講演会
  • Place of Presentation: 彦根市、滋賀県立大学
  • Year and Date: 2008-10-12
• [Presentation] Construction of a linkage map of Brassica rapa based on dot-blot-SNP markers and QTL analysis for floweringtime and leaf morphological traits (2008)
  • Author(s): Li F, Ishimaru Y, Kitashiba H, Nishio T
  • Organizer: 日本育種学会114回講演会
  • Place of Presentation: 彦根, 滋賀県立大学
  • Year and Date: 2008-10-11
• [Presentation] Construction of a linkage map of Brassica rapa based on dot-blot-SNP markers and QTL analysis for flowering time and leaf morphological traits (2008)
  • Author(s): Li F
  • Organizer: 日本育種学会114回講演会
  • Place of Presentation: 彦根市、滋賀県立大学
  • Year and Date: 2008-10-11
• [Presentation] Isolation of microspore embryogeneis specific genes in rapeseed using embryo specific promoter (2008)
  • Author(s): Sakai M, Yokoi S, Takahata Y
  • Organizer: 16th Crucifer Genetic Workshop
  • Place of Presentation: Lillehammel
  • Year and Date: 2008-09-10
• [Presentation] Isolation of microspore embryogenesis specific genes in rapeseed using embryo specific promoter (2008)
  • Author(s): Sakai Marie
  • Organizer: 16th Crucifer Genetic Workshop
  • Place of Presentation: Lillehammel, Norway
  • Year and Date: 2008-09-10
• [Presentation] シロイヌナズナ胚特異的PGD-fingerタンパク質伝子(EPS)の欠損は初期胚の致死を引き起こす (2008)
  • Author(s): 津和本亮・横井修司・高畑義人
  • Organizer: 日本育種学会113回講演会
  • Place of Presentation: 川崎, 明治大学
  • Year and Date: 2008-03-28
• [Presentation] シロイヌナズナ胚特異的PGD-fingerタンパク質(EPS)の欠損は初期胚の致死を引き起こす. (2008)
  • Author(s): 津和本亮
  • Organizer: 日本育種学会
  • Place of Presentation: 川崎市、明治大学
  • Year and Date: 2008-03-28
• [Presentation] セイヨウナタネ雄性発生胚特異的遺伝子ESPの同定および機能解析 (2007)
  • Author(s): 津和本亮・横井修司・高畑義人
  • Organizer: 日本植物学会東北支部第20回秋田大会
  • Place of Presentation: 秋田, 秋田県立大学
  • Year and Date: 2007-12-08
• [Presentation] セイヨウナタネ雄性発生胚特異的遺伝子ESPの同定および機能解析. (2007)
  • Author(s): 津和本亮
  • Organizer: 日本植物学会東北支部
  • Place of Presentation: 秋田市、秋田県立大学
  • Year and Date: 2007-12-08
• [Presentation] EMBRYOMAKER遺伝子の異所発現は子葉からの不定胚形成を誘導する (2007)
  • Author(s): 津和本亮・横井修司・高坦義人
  • Organizer: 日本育種学会112回講演会
  • Place of Presentation: 鶴岡, 山形大学
  • Year and Date: 2007-09-22
• [Presentation] EMBRYOMAKER遺伝子の異所発現は子葉からの不定胚形成を誘導する (2007)
  • Author(s): 津和本亮
  • Organizer: 日本育種学会
  • Place of Presentation: 鶴岡市、山形大学農学部
  • Year and Date: 2007-09-22
• [Presentation] Identification and analysis of andorogenesis-specific expressed sequence tags in rapeseed using suppression subtractive hybridization. (2007)
  • Author(s): Tsuwamoto R, Takahata Y
  • Organizer: The 12th International Rapeseed Congress
  • Place of Presentation: Wuhan
• [Presentation] セイヨウナタネ小胞子由来球状胚特異的ESTsの単離および発現解析 (2006)
  • Author(s): 津和本亮・高畑義人
  • Organizer: 日本育種学会110回講演会
  • Place of Presentation: 松山, 愛媛大学
  • Year and Date: 2006-09-23
• [Presentation] lack of Arabidopsis GASSHO1 and GASSHO2 genes encoding LRR-RLKs leads to the defect in cuticle during embryo stages. (2006)
  • Author(s): Tsuwamoto R, Fukuoka H, Takahata Y
  • Organizer: 15th Crucifer Genetic Workshop
  • Place of Presentation: Wageningen
• [Book] CRC Press, New York (2009)
  • Author(s): Pratap A, Gupta SK, Takahata Y
  • Publisher: Microsporogenesis and haploidy breeding. In, Biology and Breeding of Crucifers(Gupta, S.K.ed.)