High performance gene targeting for functional genomics in rice.
| Project/Area Number |
18380008
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Breeding science
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| Research Institution | National Institute for Basic Biology |
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Principal Investigator |
TERADA Rie National Institute for Basic Biology, 分子遺伝学研究部門, 助教 (30137799)
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| Project Period (FY) |
2006 – 2008
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| Project Status |
Completed (Fiscal Year 2008)
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| Budget Amount *help |
¥17,970,000 (Direct Cost: ¥15,300,000、Indirect Cost: ¥2,670,000)
Fiscal Year 2008: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2007: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
Fiscal Year 2006: ¥6,400,000 (Direct Cost: ¥6,400,000)
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| Keywords | イネ / 遺伝子ターゲティング / 相同組換え / Adh / Cre / loxP / アグロバクテリウム / 遺伝子スイッチ / T-DNA |
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| Research Abstract |
イネの遺伝子ターゲティング法を機能ゲノム学を推進する上で有効な技術として進展させることを目指して、ターゲット改変した内在遺伝子の機能をOFFから ONの状態に復帰させる、遺伝子スイッチのシステム化を、これまで遺伝子の破壊を行ってきたWaxyとアルコール脱水素酵素遺伝子(Alcohol dehydrogenase) Adh1とAdh2について行い、WaxyとAdh2についてはONの状態に復帰していることを分子レベルで確認した。また、ターゲティング法の高効率化を実現した。
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Report
(4 results)
Research Products
(30 results)
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[Presentation] Targeted Disruption of the OsDRM1a Gene, a Rice Homolog of DOMAINS REARRANGED METHYLTRANSFERASE, by Homologous Recombination.2008
Author(s)
Satoru Moritoh, Rie Terada, Yasuyo Johzuka-Hisatomi, Chang-Ho Eun, Katsushi Yamaguchi, Akemi Ono, and Shigeru Iida
Organizer
The 55th NIBB Conference Arabidopsis Workshop
Place of Presentation
Okazaki Conference Centor, aichi, Japan
Related Report
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[Presentation] Targeted Disruption of the OsDRM1a Gene, a Rice Homolog of DOMAINS REARRANGED METHYLTRANSFERASE, by Homologous Recombination2008
Author(s)
Satoru Moritoh, Rie Terada, Yasuyo Johzuka-Hisatomi, Chang-Ho Eun, Katsushi Yamaguchi, Akemi Ono, Shigeru Iida
Organizer
The 55^<th> NIBB Conference Arabidopsis Workshop
Place of Presentation
Okazaki Conference Centor, aichi, Japan
Related Report
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[Book] Rice Biology in the Genomics Era (Biotechnology in Agriculture and Forestry) H.Hirano, Y.Sano, A. Hirai, T. Sasaki(eds) (共著、分担項目、Homologous recombination-dependent gene targeting and an active DNA transposon nDart-promoted gene tagging for rice functional genomics.)(頁81-94)2008
Author(s)
Y.Johzuka-Hisatomi, M.Maekawa, K.Takagi, C-H.Eun, T.Yamauchi, Z. Shimatani, N.Ahmed, H.Urawa, K.Tsugane, R.Terada, S.Iida
Publisher
Springer
Related Report
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[Book] Homologous recombination-dependent gene targeting and an active DNA transposon nDart-promoted gene tagging for rice functional genomics. Rice Biology in the Genomics Era (Biotechnology in Agriculture and Forestry) H. Hirano, Y. Sano, A. Hirai, T. Sasaki (eds)2008
Author(s)
Y. Johzuka-Hisatomi, M. Maekawa, K. Takagi, C-H. Eun, T. Yamauchi, Z. Shimatani, N. Ahmed, H. Urawa, K. Tsugane, R. Terada, S. Iida
Total Pages
14
Publisher
Springer
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[Book] Homologous recombination-dependent gene targeting and an active DNA transposon nDart-promoted gene tagging for rice functional genomics. In Hirai A, Sano Y, Hirano H, Sasaki T(eds.), Rice Biology hl the Genomics Era:Biotechnolqgy in Agriculture and Forest2008
Author(s)
Johzuka-Hisatomi Y, Maekawa M, Takagi K, Eun C-H, Yamauchi T, Shimatani Z, Ahmed N, Urawa H, Tsugane K, Terada R, Iida S
Total Pages
14
Publisher
Springer,New York
Related Report
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