Mechanism of salinity stress effects and tolerance in C_4 plants

• Project/Area Number: 18380012
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Crop science/Weed science
• Research Institution: Nagoya University
• Principal Investigator: MIYAKE Hiroshi Nagoya University, 大学院・生命農学研究科, 教授 (60134798)
• Co-Investigator(Kenkyū-buntansha): TANIGUCHI Mitsutaka 名古屋大学, 大学院・生命農学研究科, 教授 (40231419) ; KAWASAKI Michio 弘前大学, 農学生命科学部, 准教授 (30343213)
• Project Period (FY): 2006 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥17,600,000 (Direct Cost: ¥15,200,000、Indirect Cost: ¥2,400,000); Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2007: ¥8,450,000 (Direct Cost: ¥6,500,000、Indirect Cost: ¥1,950,000); Fiscal Year 2006: ¥7,200,000 (Direct Cost: ¥7,200,000)
• Keywords: 塩ストレス / 光合成 / C_4植物 / 電子顕微鏡 / 微細構造 / 葉緑体 / 細胞・組織 / 作物 / 植物 / C4植物 / ストレス / グラナ

Research Abstract

塩ストレスは作物の生産を低下させる重要な環境問題である。本研究は塩ストレスに強いといわれるC_4植物の耐塩性を調べ、C_3植物である作物への応用をめざす。成長で比較するとC_4植物の方が耐塩性が強かったが、その原因の一つは蒸散量が少なく、塩の取り込みが少ないことであった。またC4植物は2種類の葉緑体を持つが、そのうちの維管束鞘葉緑体は耐塩性を示した。したがって、今後は維管束鞘葉緑体の耐塩性機構を明らかにすることが必要である。

Research Products

• [Journal Article] Salinity induces granal development in bundle sheath chloroplasts of NADP-malic enzyme type C_4 plants (2009)
  • Author(s): Omoto E, Kawasaki M, Taniguchi M, Miyake H.
  • Journal Title: Plant Production Science 12 Pages: 199-207
  • NAID: 10025922014
  • Peer Reviewed
• [Journal Article] Differential positioning of C_4 mesophyll and bundle sheath chloroplasts : recovery of chloroplast positioning requires the actomyosin system (2009)
  • Author(s): Kobayashi H, Yamada M, Taniguchi M, Kawasaki M, Sugiyama T, Miyake H.
  • Journal Title: Plant and Cell Physiology 50 Pages: 129-140
  • NAID: 10027336734
  • Peer Reviewed
• [Journal Article] Salinity induces granal development in bundle sheath chloroplasts of NADP-malic enzyme type C4 plants (2009)
  • Author(s): Omoto, E., et al.
  • Journal Title: Plant Production Science 12 Pages: 199-207
  • Peer Reviewed
• [Journal Article] Differential positioning of C4 mesophyll and bundle sheath chloroplasts : recovery of chloroplast positioning requires the actomyosin system. (2009)
  • Author(s): Kobayashi, H., et al.
  • Journal Title: Plant and Cell Physiology 50 Pages: 129-140
  • Peer Reviewed
• [Journal Article] Gene expression and accumulation of Rubisco in bundle sheath and mesophyll cells during leaf development and senescence in rice, a C_3 plant (2008)
  • Author(s): Tsutsumi K, Kawasaki M, Taniguchi M, Miyake H.
  • Journal Title: Plant Production Science 11 Pages: 336-343
  • NAID: 110006792422
  • Peer Reviewed
• [Journal Article] Correlation between chloroplast ultrastructure and chlorophyll fluorescence characteristics in the leaves of rice (Oryza sativa L.) grown under salinity (2008)
  • Author(s): Yamane K, Kawasaki M, Taniguchi M, Miyake H.
  • Journal Title: Plant Production Science 11 Pages: 139-145
  • NAID: 110006546701
  • Peer Reviewed
• [Journal Article] Gene expression and accumulation of Rubisco in bundle sheath and mesophyll cells during leaf development and senescence in rice, a C3 plant. (2008)
  • Author(s): Tsutsumi, K., et al.
  • Journal Title: Plant Production Science 11 Pages: 336-343
  • Peer Reviewed
• [Journal Article] Correlation between chloroplast ultrastructure and chlorophyll fluorescence charactelistics in the leaves of rice (Oryza sativa L.) grown under salinity. (2008)
  • Author(s): Yamane K, et. al.
  • Journal Title: Plant Production Science 11 Pages: 139-145
  • Peer Reviewed
• [Journal Article] Gene expression and protein content of Rubisco in bundle sheath and mesophyll cells during leaf development and senescence of a C_3 plant rice. (2008)
  • Author(s): Tsutsumi K, et. al.
  • Journal Title: Plant Production Science 11(In press)
  • Peer Reviewed
• [Journal Article] Structural and functional differentiation of bundle sheath and mesophyll cells in the lamina jointof rice compared with that in the corresponding region of the liguleless genotype (2007)
  • Author(s): Tsutsumi K, Kawasaki M, Taniguchi M, Itani T, Maekawa M, Miyake H
  • Journal Title: Plant Production Science 10 Pages: 346-356
  • Peer Reviewed
• [Journal Article] Structural and functional differentiation between bundle sheath and mesophyll cells in the lamina joint of rice as compared with that in the corresponding region of the liguleless genotype. (2007)
  • Author(s): Tsutsumi K, et. al.
  • Journal Title: Plant Production Science 10 Pages: 346-356
  • Peer Reviewed
• [Journal Article] Overexpression of RC12A decreases Na^+ uptake and mitigates salinity-induced damages in Arabidopsis thaliana plants (2006)
  • Author(s): Mitsuya S, Taniguchi M, Miyake H, Takabe T.
  • Journal Title: Physiologia Plantarum 128 Pages: 95-102
  • Peer Reviewed
• [Journal Article] Salinity stress induces granal development in bundle sheath chloroplasts of maize, an NADP-malic enzyme type C_4 plant (2006)
  • Author(s): Hasan R, Kawasaki M, Taniguchi M, Miyake H.
  • Journal Title: Plant Production Science 9 Pages: 256-265
  • NAID: 110004775673
  • Peer Reviewed
• [Journal Article] Salinity stress induces granal development in bundle sheath chloroplasts of maize, an NADP-malic enzyme type C_4 plant. (2006)
  • Author(s): Hasan, R
  • Journal Title: Plant Production Science 9 Pages: 256-265
• [Journal Article] Overexpression of RC12A decreases Na^+ uptake and mitigates salinity-induced damages in Arabidopsis thaliana plants. (2006)
  • Author(s): Mitsuya, S.
  • Journal Title: Physiologia Plantarum 128 Pages: 95-102
• [Journal Article] Differential sensitivity of rice cultivars to salinity and its relation to ion accumulation and root tip structure
  • Author(s): Ferdose J, Kawasaki M, Taniguchi M, Miyake H.
  • Journal Title: Plant Production Science 12(in press)
  • NAID: 130004462191
  • Peer Reviewed
• [Journal Article] Antioxidant capacity and damages caused by salinity stress in apical and basal regions of rice leaf
  • Author(s): Yamane K, Mitsuya S, Kawasaki M, Taniguchi M, Miyake H.
  • Journal Title: Plant Production Science 12(in press)
  • NAID: 10025922462
  • Peer Reviewed
• [Journal Article] X線マイクロCTスキャナによる日本型イネ下位枝梗着生部における穂首維管束走向の非破壊的測定
  • Author(s): 前田英三・三宅博
  • Journal Title: 日本作物学会紀事 78(3)(in press)
  • Peer Reviewed
• [Presentation] 環境ストレスに応答したC_4植物葉肉葉緑体の集合運動-他のオルガネラとの相互作用および光シグナルに対する検討- (2009)
  • Author(s): 谷口光隆・山田雅大・川崎通夫・三宅博
  • Organizer: 日本作物学会第227回講演会
  • Place of Presentation: つくば
• [Presentation] ネラミナジョイントの維管束鞘細胞と葉肉細胞におけるRubiscoの発現 (2009)
  • Author(s): 堤浩一・川崎通夫・谷口光隆・三宅博
  • Organizer: 日本作物学会第227回講演会
  • Place of Presentation: つくば
• [Presentation] 作物ストレス研究における電子顕微鏡技術の応用と展開 (2009)
  • Author(s): 三宅博
  • Organizer: 日本作物学会第227回講演会
  • Place of Presentation: つくば
• [Presentation] C_4植物葉肉細胞における環境ストレスに応答した葉緑体集合運動の光応答性 (2009)
  • Author(s): 山田雅大・谷口光隆・川崎通夫・三宅博
  • Organizer: 第50回日本植物生理学会年会
  • Place of Presentation: 名古屋
• [Presentation] C4植物葉肉細胞における環境ストレスに応答した葉緑体集合運動の光応答性 (2009)
  • Author(s): 山田雅大, 他
  • Organizer: 第50回日本植物生理学会年会
  • Place of Presentation: 名古屋
• [Presentation] 環境ストレスに応答したC4植物葉肉葉緑体の集合運動-他のオルガネラとの相互作用および光シグナルに対する検討- (2009)
  • Author(s): 谷口光隆, 他
  • Organizer: 日本作物学会第227回講演会
  • Place of Presentation: つくば
• [Presentation] Effects of salinity on the growth and root tip structure of rice cultivars (2008)
  • Author(s): Ferdose J, Taniguchi M, Kawasaki M, Miyake H.
  • Organizer: 5th International Crop Science Congress & Exhibition
  • Place of Presentation: Jeju, Korea
  • Year and Date: 2008-04-18
• [Presentation] 環境ストレスに応答したC_4植物葉肉葉緑体の集合運動 (2008)
  • Author(s): 谷口光隆・山田雅大・川崎通夫・三宅博
  • Organizer: 日本作物学会第226回講演会
  • Place of Presentation: 神戸
• [Presentation] Cellular distribution of hydrogen peroxide in root cells in rice plants exposed to salinity (2008)
  • Author(s): Ferdose J, Kawasaki M, Taniguchi M, Miyake H.
  • Organizer: 日本作物学会第226回講演会
  • Place of Presentation: 神戸
• [Presentation] Effects of salinity stress on the fine structure of bundle sheath chloroplasts of C_4 plants (2008)
  • Author(s): Omoto E, Kawasaki M, Taniguchi M, Miyake H
  • Organizer: 5th International Crop Science Congress & Exhibition
  • Place of Presentation: Jeju, Korea
• [Presentation] 環境ストレスに伴うC_4植物葉肉葉緑体の光逃避運動 (2008)
  • Author(s): 山田雅大・谷口光隆・川崎通夫・三宅博
  • Organizer: 第49回日本植物生理学会年会
  • Place of Presentation: 札幌
• [Presentation] Effects of salinity stress on the fine structure of bundle sheath chloroplasts of C4 plants. (2008)
  • Author(s): Omoto, E., et al.
  • Organizer: 5th International Crop Science Congress & Exhibition
  • Place of Presentation: Jeju, Korea
• [Presentation] Effects of salinity on the growth and root tip structure of ricecultivars. (2008)
  • Author(s): Ferdose, J., et al.
  • Organizer: 5th International Crop Science Congress & Exhibition
  • Place of Presentation: Jeju, Korea
• [Presentation] Cellular distribution of hydrogen peroxide in root cells in riceplants exposed to salinity. (2008)
  • Author(s): Ferdose, J., et al.
  • Organizer: 日本作物学会第226回講演会
  • Place of Presentation: 神戸
• [Presentation] 環境ストレスに応答したC4植物葉肉葉緑体の集合運動 (2008)
  • Author(s): 谷口光隆, 他
  • Organizer: 日本作物学会第226回講演会
  • Place of Presentation: 神戸
• [Presentation] Intracellular re-arrangement of C_4 mesophyll chloroplasts in response to environmental stress. (2007)
  • Author(s): Yamada M, et. al.
  • Organizer: The 6th Asian Crop Science Association Conference
  • Place of Presentation: Bangkok, Thailand
  • Year and Date: 2007-11-09
• [Presentation] Antioxidant capacity and damages caused by salinity stress in the leaf tissues with different age in rice plants (Oryza sativa L.). (2007)
  • Author(s): Yamane K, et. al.
  • Organizer: The 2nd International Conference on Rice for the Future
  • Place of Presentation: Bangkok, Thailand
  • Year and Date: 2007-11-08
• [Presentation] Effects of NaCl on the growth, ion accumulation and seminal root capstructure during different growth stages of rice varieties differing in salinity resistance. (2007)
  • Author(s): Ferdose J, ら
  • Organizer: 日本作物学会第224回講演会
  • Place of Presentation: 金沢
  • Year and Date: 2007-09-26
• [Presentation] 乾燥ストレスと過酸化水素はNADP-ME型C_4植物であるトウモロコシの維管束鞘葉緑体のグラナの発達を誘導する (2007)
  • Author(s): 加藤剛志, ら
  • Organizer: 日本作物学会第224回講演会
  • Place of Presentation: 金沢
  • Year and Date: 2007-09-26
• [Presentation] Differential expression of photosynthetic genes between bundle sheath and mesophyll cell of leaf blades of a C_3 plant rice during development and senescence (2007)
  • Author(s): Tsutsumi K, Kawasaki M, Taniguchi M, Miyake H.
  • Organizer: The 2nd International Conference on Rice for the Future
  • Place of Presentation: Bangkok, Thailand
• [Presentation] Functional analysis of plasma membrane protein 3 (PMP3) homologs in salt tolerance of rice and Arabidopsis plants (2007)
  • Author(s): Mitsuya S, Yamane K, Miyake H, Takabe H.
  • Organizer: The 2nd International Conference on Rice for the Future
  • Place of Presentation: Bangkok, Thailand
• [Presentation] Antioxidant capacity and damages caused by salinity stress in the leaf tissues with different age in rice plants (Oryza sativa L.) (2007)
  • Author(s): Yamane K, Kawasaki M, Taniguchi M, Miyake H.
  • Organizer: The 2nd International Conference on Rice for the Future
  • Place of Presentation: Bangkok, Thailand
• [Presentation] Aluminum localization and the detoxification mechanism of aluminum in Eddo roots (2007)
  • Author(s): Kawasaki M, Takatsuji A, Taniguchi M, Miyake H.
  • Organizer: The 6th Asian Crop Science Association Conference
  • Place of Presentation: Bangkok, Thailand
• [Presentation] Intracellular re-arrangement of C_4 mesophyll chloroplasts in response to environmental stress (2007)
  • Author(s): Yamada M, Taniguchi M, Kawasaki M, Miyake H.
  • Organizer: The 6th Asian Crop Science Association Conference
  • Place of Presentation: Bangkok, Thailand
• [Presentation] 塩ストレスがC_4植物の維管束鞘葉緑体の構造に及ぼす影響 (2007)
  • Author(s): 大元英司・川崎通夫・谷口光隆・三宅博
  • Organizer: 日本作物学会第224回講演会
  • Place of Presentation: 金沢
• [Presentation] 乾燥ストレスと過酸化水素はNADP-ME型C4植物であるトウモロコシの維管束鞘葉緑体のグラナの発達を誘導する (2007)
  • Author(s): 加藤剛志・川崎通夫・谷口光隆・三宅博
  • Organizer: 日本作物学会第224回講演会
  • Place of Presentation: 金沢
• [Presentation] Effects of NaCl on the growth, ion accumulation and seminal root cap structure during different growth stages of rice varieties differing in salinity resistance (2007)
  • Author(s): Ferdose J, Kawasaki M, Taniguchi M, Miyake H.
  • Organizer: 日本作物学会第224回講演会
  • Place of Presentation: 金沢
• [Presentation] イネ葉身の発達過程、老化過程における維管束鞘細胞と葉肉細胞の機能分化に関する研究 (2007)
  • Author(s): 堤浩一・川崎通夫・谷口光隆・三宅博
  • Organizer: 日本作物学会第224回講演会
  • Place of Presentation: 金沢
• [Presentation] 環境変動に応答したC_4植物葉緑体の細胞内配向性の変化 (2007)
  • Author(s): 山田雅大・谷口光隆・川崎通夫・三宅博
  • Organizer: 第48回日本植物生理学会年会
  • Place of Presentation: 松山
• [Presentation] イネラミナジョイントにおける組織分化と光合成遺伝子の発現 (2006)
  • Author(s): 堤浩一・川崎通夫・谷口光隆・猪谷富雄・前川雅彦・三宅博
  • Organizer: 日本作物学会東海支部第137回講演会
  • Place of Presentation: 名古屋
  • Year and Date: 2006-09-06
• [Presentation] 塩ストレスによる数種C_4植物の微細構造変化 (2006)
  • Author(s): 大元英司・川崎通夫・谷口光隆・三宅博
  • Organizer: 日本作物学会東海支部第137回講演会
  • Place of Presentation: 名古屋
  • Year and Date: 2006-09-06
• [Presentation] 塩ストレスによるイネ葉緑体微細構造変化とクロロフィル蛍光変化の関係 (2006)
  • Author(s): 山根浩二・川崎通夫・谷口光隆・三宅博
  • Organizer: 日本作物学会東海支部第137回講演会
  • Place of Presentation: 名古屋
  • Year and Date: 2006-09-06
• [Presentation] 塩ストレスが引き起こすイネ葉緑体微細構造変化とクロロフィル蛍光変化の関係 (2006)
  • Author(s): 山根浩二・川崎通夫・谷口光隆・三宅博
  • Organizer: 日本作物学会第222回講演会
  • Place of Presentation: 香川
• [Presentation] シロイヌナズナRCI2A遺伝子の過剰発現によるNa^+吸収の減少および塩ストレス障害の緩和 (2006)
  • Author(s): 三屋史朗・谷口光隆・三宅博・高倍鉄子
  • Organizer: 第47回日本植物生理学会年会
  • Place of Presentation: つくば
• [Presentation] 組織の齢の違いが塩ストレス下における抗酸化酵素活性に及ぼす影響 (2006)
  • Author(s): 山根浩二・川崎通夫・谷口光隆・三宅博
  • Organizer: 第47回日本植物生理学会年会,
  • Place of Presentation: つくば
• [Book] 作物学概論 (2008)
  • Author(s): 三宅博
  • Publisher: 朝倉書店
• [Book] 作物の形態研究法 (2008)
  • Author(s): 前田英三・三宅博・井上吉雄
  • Total Pages: 132
  • Publisher: 日本作物学会
• [Book] 「作物学概論」(大門弘幸編) (2008)
  • Author(s): 三宅 博
  • Publisher: 朝倉書店
• [Remarks]
  • URL: http://www.agr,nagoya-u.ac.jp/-shigen/