| Project/Area Number |
18380023
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Horticulture/Landscape architecture
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| Research Institution | Kyoto University |
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Principal Investigator |
YAZAWA Susumu Kyoto University, Graduate school of Agriculture, Professor (90026550)
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| Co-Investigator(Kenkyū-buntansha) |
WATANABE Tatsuo University of Shizuoka, School of Food and Nutritional Sciences, Professor (10210915)
HOSOKAWA Munetaka Kyoto University, Graduate School of Agriculture, Assistant Professor (40301246)
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| Project Period (FY) |
2006 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥15,840,000 (Direct Cost: ¥14,400,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2007: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2006: ¥9,600,000 (Direct Cost: ¥9,600,000)
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| Keywords | Capsiconinoid / Capsiconiate / DNA marker / Capsinoid / Fruit of pepper for salad vegetable / Capsicum baccatum var. praetermissum / Identification of novel non-pungent compounds / 新規非辛味成分の同定 / トウガラシ / 機能性物質 / 新規生食用野菜 / 品種育成 |
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| Research Abstract |
1. Coniferyl esters, capsiconiate and dihydrocapsiconiate were isolated from the fruits of the pepper, Capsicum baccatum var. praetermissum Their structures were determined by spectroscopic method to be coniferyl 8-methyl -6-nonanoate (capsiconiate) and coniferyl 8-nonanoate (dihydrocapsiconiate).
2. These compounds can be artificially synthesized by the lipase-catalyzed condensation of coniferyl alcohol with its corresponding fatty acid derivative.
3. The agonist activity of the esters for transient receptor potential vanilloid 1 (TRPV1) was evaluated by conductivity an analysis of the intracellular calcium concentrations in TRPV1-expressing HEK293 cells. The EC50 values of capsiconiate and dihydrocapsiconiate were 3.2 and 4.2 μM, respectively.
4. Capsiconinoid in the fruit increases 3 weeks after anthesis and this compounds in the red ripening fruit. Capsiconinoid was biosynthesized in the cells of the placenta.
5. Acyltransferase gene marker was used breeding of new non-pungent pepper with soft-juicy fruits.
6. The pepper for salad with the fruit vegetables containing 1000 ppm (D. W.) capsinoid was breeded. The harvest of the new variety was 4-5 kg per plant.
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