Analysis and application of polycistronic translation of plant pararetrovirus

• Project/Area Number: 18380029
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Plant pathology
• Research Institution: The University of Tokyo
• Principal Investigator: SUZUKI Masashi The University of Tokyo, 大学院・新領域創成科学研究科, 准教授 (40282694)
• Co-Investigator(Kenkyū-buntansha): 宇垣 正志 東京大学, 大学院・新領域創成科学研究科, 教授 (20323438) ; 日比 忠明 玉川大学, 学術研究所, 教授 (50261954)
• Co-Investigator(Renkei-kenkyūsha): UGAKI Masashi 東京大学, 大学院・新領域創成科学研究科, 教授 (20323438) ; HIBI Tadaaki 法政大学, 生命科学部, 教授 (50261954)
• Project Period (FY): 2006 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥17,630,000 (Direct Cost: ¥15,200,000、Indirect Cost: ¥2,430,000); Fiscal Year 2008: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2007: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000); Fiscal Year 2006: ¥7,100,000 (Direct Cost: ¥7,100,000)
• Keywords: 植物パラレトロウイルス / ポリシストロニック翻訳 / ダイズ退緑斑紋ウイルス / 翻訳トランスアクティベーター / 翻訳 / 植物ウイルス

Research Abstract

植物パラレトロウイルスのもつ、1本のmRNA から複数のタンパク質を翻訳するポリシストロニック翻訳機構の解明を行った。その結果、既知のカリフラワーモザイクウイルス P6 と同様、ダイズ退緑斑紋ウイルスP6 のポリシストロニック翻訳を活性化する翻訳トランスアクティベーター(TAV)活性を本研究で初めて証明した。さらに、CaMV P6のTAV活性は非宿主でも認められたのに対し、SbCMV P6のTAV活性は宿主特異性があることが示された。

Research Products

• [Journal Article] NTH201, a novel class II KNOTTED1-like protein, facilitates the cell-to-cell movement of Tobacco mosaic virus in tobacco (2008)
  • Author(s): Yoshii A., Shimizu T., Yoshida, A., Hamada, K., Sakurai, K., Yamaji Y., Suzuki M., Namba, S. and Hibi T.
  • Journal Title: Molecular Plant-Microbe Interaction 21(5) Pages: 586-596
  • Peer Reviewed
• [Journal Article] stunt virus 2b cistron plays a role in viral local and systemic accumulation and virulence in Nicotiana benthamiana (2008)
  • Author(s): Netsu, O., Hiratsuka, K., Kuwata, S., Hibi, T., Ugaki, M. and Suzuki, M.
  • Journal Title: Archives of Virology 153(9) Pages: 1731-1735
  • Peer Reviewed
• [Journal Article] Characterization of a defective RNA derived from RNA 3 of the Y strain of cucumber mosaic virus. (2008)
  • Author(s): Takeshita, M., Matsuo, Y., Yoshikawa, T., Suzuki, M., Furuya, N., Tsuchiya, K. and Takanami, Y.
  • Journal Title: Archives of Virology 153 Pages: 579-583
  • Peer Reviewed
• [Journal Article] Peanut stunt virus 2b cistron plays a role in viral local and systemic accumulation and virulence in Nicotiana benthamiana (2008)
  • Author(s): Netsu, U., Hiratsuka, K., Kuwata. S., Hibi, T., Ugaki, M. and Suzuki M.
  • Journal Title: Archives of Virology 153(9) Pages: 1731-1735
  • Peer Reviewed
• [Journal Article] Characterization of a defective RNA derived from RNA 3 of the Y strain ofcucumber mosaic virus (2008)
  • Author(s): Takeshita, M., Matsuo, Y., Yoshikawa, T., Suzuki, M., Furuya, N., Tsuchiya, K. and Taakanami, Y.
  • Journal Title: Archives of Virology 153 Pages: 579-583
  • Peer Reviewed
• [Journal Article] In vivo interaction between Tobacco mosaic virus RNA-dependent RNA polymerase and host translational elongation factor 1A (2006)
  • Author(s): Yamaji Y, Kobayashi T, Hamada K, Sakurai K, Yoshii A, Suzuki M, Namba S, Hibi T
  • Journal Title: Virology 347 Pages: 100-108
• [Journal Article] A single amino acid in the RNA-dependent RNA polymerase of Plantago asiatica mosaic virus contributes to systemic necrosis. (2006)
  • Author(s): Ozeki J, Takahashi S, Komatsu K, Kagiwada S, Yamashita K, Mori T, Hirata H, Ymaji Y, Ugaki M, Namba S
  • Journal Title: Archives of Virology 151・10 Pages: 2067-2075
• [Journal Article] Positive selection acting on a surface membrane protein of the plant-pathogenic phytoplasmas. (2006)
  • Author(s): Kakizawa S, Oshima K, Jung HY, Suzuki S, Nishigawa H, Arashida R, Miyata S, Ugaki M, Kishino H, Namba S
  • Journal Title: Journal of Bacteriology 188・9 Pages: 3424-3428
• [Journal Article] Interaction between the membrane protein of a pathogen and insect microfilament complex determines insect-vector specificity. (2006)
  • Author(s): Suzuki S, Oshima K, Kakizawa S, Arashida R, Jung HY, Yamaji Y, Nishigawa H, Ugaki M, Namba S
  • Journal Title: Proc. Natl. Aca. Sci. USA. 103・11 Pages: 4252-4257
• [Journal Article] The efficiency of interference of Potato virus X infection depends on the target gene. (2006)
  • Author(s): Takahashi S, Komatsu K, Kagiwada S, Ozeki J, Mori T, Hirata H, Yamaji Y, Ugaki M, Namba S
  • Journal Title: Virus Research 116・2 Pages: 214-217
• [Journal Article] Impact of a defective RNA 3 from Cucumber mosaic virus on helper virus infection dynamics
  • Author(s): Takeshita, M., Matsuo, Y., Suzuki, M., Furuya, N., Tsuchiya, K. and Takanami, Y.
  • Journal Title: Virology (印刷中)
  • Peer Reviewed
• [Journal Article] NTH201, a novel class II KNOTTED1-like protein, facilitates the cell-to-cell movement of Tobacco mosaic virus in tobacco.
  • Author(s): Yoshii A shimizu T., Yoshida, A., Hamada, K., Yamaji Y,. Auzuki M., Namba. And
  • Journal Title: Molecular Plant-Microbe Interaction 21(5) Pages: 585-596
  • Peer Reviewed
• [Journal Article] Impact of adefective RNA3 from Cucumber mosaic virus on helper virus infection dynamics.
  • Author(s): Takeshita, M., Matsuo, Y., Suzuki, M., Furuya, N., Tsuchiya, K. and Takanami. Y.
  • Journal Title: Virology (印刷中)
  • Peer Reviewed
• [Presentation] ラッカセイわい化ウイルス(PSV) 2bタンパク質のN末端領域欠失によるRNAサイレンシングのサプレッサー能への影響 (2009)
  • Author(s): 根津修・平塚和之・桑田茂・日比忠明・宇垣正志・鈴木匡
  • Organizer: 日本植物病理学会
  • Place of Presentation: 山形市
• [Presentation] Functions of the 2b protein of Peanut stunt virus in viral replication, movement and pathogenicity. XIV (2008)
  • Author(s): Netsu, O., Hiratsuka, K., Kuwata, S., Hibi, T., Ugaki, M. and Suzuki, M.
  • Organizer: International Congress of Virology
  • Place of Presentation: イスタンブール
• [Presentation] Phylogenetic analysis of Tomatoyellow leaf curl virus isolates occurringin central Japan. XIV (2008)
  • Author(s): Ueno, T., Suzuki, M. and Ugaki, M.
  • Organizer: International Congress of Virology
  • Place of Presentation: イスタンブール
• [Presentation] Functions of the 2b protein of Peanut stunt virus in viral replication, movement and pathogenicity. (2008)
  • Author(s): Netsu, U., Hiratsuka, K., Kuwata, S., Hibi, T., Ugaki, M. and Suzuki M.
  • Organizer: XIV. International Congress of Virology
  • Place of Presentation: イスタンブール
• [Presentation] ラッカセイわい化ウイルス(PSV) 2bタンパク質のRNAサイレンシングのサプレッサー能解析 (2007)
  • Author(s): 根津修・平塚和之・桑田茂・日比忠明・宇垣正志・鈴木匡
  • Organizer: 日本植物病理学会
  • Place of Presentation: 宇都宮市
• [Presentation] キュウリモザイクウイルス(CMV) 2bタンパク質とジャガイモYウイルス(PVY) HC-ProとのRNAサイレンシング抑制能の比較 (2007)
  • Author(s): 塩田聡・大谷肇・根津修・鈴木匡・桑田茂
  • Organizer: 日本植物病理学会
  • Place of Presentation: 宇都宮市