Ultrastructural and biochemical analysis of pathogenicity factors in plant diseases

• Project/Area Number: 18380033
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Plant pathology
• Research Institution: Kobe University
• Principal Investigator: PARK Pyoyun Kobe University, 大学院・農学研究科, 教授 (20147094)
• Co-Investigator(Kenkyū-buntansha): NAKAYASHIKI Hitoshi 神戸大学, 大学院・農学研究科, 准教授 (50252804) ; IKEDA Kenichi 神戸大学, 自然科学系先端融合研究環重点研究部, 助教 (40437504)
• Project Period (FY): 2006 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥11,320,000 (Direct Cost: ¥9,700,000、Indirect Cost: ¥1,620,000); Fiscal Year 2008: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000); Fiscal Year 2007: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2006: ¥4,300,000 (Direct Cost: ¥4,300,000)
• Keywords: 感染生理 / 細胞 / 組織 / 生理活性 / 免疫学 / 病理学 / 感染症 / 細胞・組織 / 活性酸素 / 細胞外物質 / 電子顕微鏡 / 過酸化脂質 / 細胞膜変性 / 細胞接着因子

Research Abstract

病原菌の生産する宿主特異的毒素、活性酸素、細胞外物質の役割を明らかにした。毒素は感受性植物に罹病性反応だけでなく、抵抗性・修復反応といった予想外の細胞応答を引起こした。侵入菌糸に生じる活性酸素の生成を止めると、感染を阻止できた。菌が分泌する細胞外物質は菌と植物表面に間に集積するが、この物質は宿主粘着因子からなることが分かった。この因子を酵素で分解すると、菌は宿主から剥離し病害が抑制された

Research Products

• [Journal Article] The extracellular matrix produced from Alternaria alternata Japanese pear pathotype plays a possible role of adhesion on the surfaces of host leaves during plant infection. J. Electron Micrsco (2009)
  • Author(s): Hyon,G-S. Muranaka, Y. Ikeda, K. Inoue, K. Hosogi, N. Meguro H. Yamada T. Hida, S. Suzuki, T. and Park, P
  • Journal Title: Technol. Med. Biol (in press)
  • Peer Reviewed
• [Journal Article] Ultrastructural analysis of responses of host and fungal cells during plant infction (2008)
  • Author(s): Park, P. and Ikeda, K
  • Journal Title: J. Gen. Plant Pathol 74 Pages: 2-14
  • Peer Reviewed
• [Journal Article] Ultrastructuralidentification of ion-leakage sites in host cellstreated with AK-toxin I from Alternaria alternata Japanese pear pathotype (2008)
  • Author(s): Hosogi, N. Hyon, G-S. Inoue, K. Jiang, S. Ikeda, K. and Park, P
  • Journal Title: J. Electron Micrsco. Technol. Med. Biol 21 Pages: 172-178
  • Peer Reviewed
• [Journal Article] Ultrastructural study on acibenzolar-S-methyl-induced scab resistance in epidermal pectin layers of Japanese pear leaves (2008)
  • Author(s): Jiang, S., Park P. and Ishii, H
  • Journal Title: Phytopathology 98 Pages: 585-591
  • Peer Reviewed
• [Journal Article] NoxA activation by the small GTPase RacA is required to maintain a mutualistic symbiotic association Epichloe festucae and perennial ryegrass (2008)
  • Author(s): Tanaka, A. Takemoto, D. Hyon, G-S. Park, P. and Scott, B
  • Journal Title: Molecular Microbiology 68 Pages: 1165-1178
  • Peer Reviewed
• [Journal Article] Reactions of Lotus japonicus ecotypes and mutants to root parasitic plants (2008)
  • Author(s): Kubo E, Park, P. and Sugimoto, Y
  • Journal Title: J. Plant Physiol 166 Pages: 352-362
  • Peer Reviewed
• [Journal Article] Ultrastructural analysis of responses of host and fungal cellsduring plant infection (2008)
  • Author(s): Park, P. Ikeda, K.
  • Journal Title: J. Gen. Plant Pathol. 74 Pages: 2-14
  • Peer Reviewed
• [Journal Article] Ultrastructural identification of ion-leakage sites in host cells treated with AK-toxin l from Alternaria alternata Japanese pear pathotype (2008)
  • Author(s): Hosogi, N., Hyon, G-S., Inoue, K., Jiang, S., Ikeda, K., Park, P.
  • Journal Title: J. Elctron Micrsco. Technol. Med. Biol. 21 Pages: 172-178
  • Peer Reviewed
• [Journal Article] Ultrastructural study on acibenzolar-S-methyl-induced scabresistance in epidermal pectin layers of Japanese pear leaves. (2008)
  • Author(s): Jiang, S., Park p., Ishii, H.
  • Journal Title: Phytopathology 98 Pages: 585-591
  • Peer Reviewed
• [Journal Article] NoxA activation by the small GTPase RacA is required tomaintain a mutualistic symbiotic association Epichloe festucae and perennial ryegrass. (2008)
  • Author(s): Tanaka, A., Takemoto, D., Hyon, G-S. Park, P., Scott, B.
  • Journal Title: Molecular Microbiology 68 Pages: 1165-1178
  • Peer Reviewed
• [Journal Article] Reactions of Lotus japonicus ecotypes and mutants to rootparasitic plants. (2008)
  • Author(s): Kubo E, Park, P., Sugimoto, Y.
  • Journal Title: Journal of Plant Physiology 166 Pages: 353-362
  • Peer Reviewed
• [Journal Article] Ultrastructural analysis of responses of host and fungal cells during plant infection. (2008)
  • Author(s): Park, P. and Ikeda, K.
  • Journal Title: Journal of General Plant Pathology 74 Pages: 2-14
  • NAID: 10029715176
  • Peer Reviewed
• [Journal Article] Ultrastructural study on scab resistance expressed in epidermal pectin layers of pear leaves (2007)
  • Author(s): Jiang, S. Park, P. Ishii, H
  • Journal Title: J. Gen. Plant Pathol 73 Pages: 314-323
  • Peer Reviewed
• [Journal Article] Acibenzolar-S-methyl primes cell wall strengthening genes and reactive oxygen species forming scavenging enzymes in cucumber after fungal pathogen attack (2007)
  • Author(s): Deepak, S. A. Ishii, H. Park, P
  • Journal Title: Physiol Mol Plant Pathol 69 Pages: 52-61
  • Peer Reviewed
• [Journal Article] Immunohistochemical and cyto- chemical analysis of extracellular matrix produce from Venturia nashicola (2007)
  • Author(s): Jiang, S. Park P. Ishii, H
  • Journal Title: scab fungus on the surfaces of susceptible Japanese pear leaves. J. Electron Micrsco. Technol. Med. Biol 21 Pages: 7-11
  • Peer Reviewed
• [Journal Article] 2007) Extracellular matrix (ECM) of Magnaporthe oryzae may have role in host adhesion during fungal penetration and is digested by matrix metalloproteases (2007)
  • Author(s): Inoue, K. Suzuki, T. Ikeda, K. Jiang, S. Hosogi, N.Hyon, G-S.,Hida, S. Yamada, T. and Park, P
  • Journal Title: J Gen. Plant. Pathol 73 Pages: 388-398
  • Peer Reviewed
• [Journal Article] A novel staining method for thin sections and en bloc tissue of rat kidney fixed with glutaraldehyde and osmium tetroxide using methanolic hafnium chloride (2007)
  • Author(s): Horiuchi, Y. Okumoto, K. Akahoshi, Y. Minamoto, G.. Onoe, T. Ikeda, K. and Park, P
  • Journal Title: J. Electron Micrsco. Technol. Med. Biol 21 Pages: 21-28
  • Peer Reviewed
• [Journal Article] Oxygen-bubbly glutaraldehyde fixation. J (2007)
  • Author(s): Park, P
  • Journal Title: Electron Micrsco. Technol. Med. Biol(in Japanese) 21 Pages: 31-37
  • Peer Reviewed
• [Journal Article] Ultrastructural study on scab resistance expressed in epidermal pectin layers of pear leaves. (2007)
  • Author(s): Jiang S, Park P, Ishii, H
  • Journal Title: Journal of General Plant Pathology 73 Pages: 314-323
  • Peer Reviewed
• [Journal Article] Extracellular matrix(ECM)of Magnaporthe oryzae may have role in host adhesion during fungal penetration and is digested by matrix metalloproteases. (2007)
  • Author(s): Inoue, K., Suzuki, T., Ikeda, K., Jiang, S., Hosogi, N., Hyon, G-S. Hida, S., Yamada, T., Park, P.
  • Journal Title: Journal of General Plant Pathology 73 Pages: 388-398
  • Peer Reviewed
• [Journal Article] Reactive oxygen species(ROS) generation and the ROS-induced lipid peroxidation, asso-ciated with plasma membrane modifica- tion (2006)
  • Author(s): Shimizu,N.,Hosogi, N., Hyon, G., Jiang, S., Inoue, K., and Park, P
  • Journal Title: were caused in host cells by AK- toxin I from Alternaria alternate Japanese pear pathotype. J. Gen. Plant Pathol 72 Pages: 6-15
  • Peer Reviewed
• [Journal Article] A novel role of reactive oxygen species in regulating a fungal-plant mutualistic interaction (2006)
  • Author(s): Tanaka, A. Christensen, M. J. Takemoto, D., Park, P., and Scott, B
  • Journal Title: Plant Cell 18 Pages: 1052-1066
  • Peer Reviewed
• [Journal Article] Ultrastructural analysis of cell responses of host cells to pathogen infection (2006)
  • Author(s): Park, P
  • Journal Title: J. Gen. Plant Pathol 72 Pages: 404-407
  • NAID: 110006189056
  • Peer Reviewed
• [Journal Article] Reactive oxygen species (ROS) generation and the ROS-induced lipid peroxidation, associated with plasma membrane modification, were caused in host cells by AK-toxin I from Alternaria alternata Japanese pear pathotype. (2006)
  • Author(s): Shimizu N, Hosogi N, Hyon G, Jiang S, Inoue K, Park P
  • Journal Title: J. Gen. Plant Pathol. 72 Pages: 6-15
• [Presentation] 各種糸状菌の胞子発芽過程における貯蔵物質の代謝変動 (2008)
  • Author(s): 目黒紘子, 井上加奈子, 池田健一, 朴杓允
  • Organizer: 平成20年度日本植物病理学会関西部会
  • Place of Presentation: 和歌山
  • Year and Date: 2008-09-19
• [Presentation] いもち病菌におけるハイドロフォビン(Mpg1)の接着能力への影響 (2008)
  • Author(s): 井上加奈子、池田健一、中屋敷均,朴杓允
  • Organizer: 第8回糸状菌分子生物学コンファレンス
  • Place of Presentation: 金沢
• [Presentation] Pathological roles of reactive oxygen species at penetration pegs of Alternaria alternata Japanese pear pathotype during plant infection (2008)
  • Author(s): Hyon,G-S. Morita, Y. Hosogi, N. Ikeda, K. Nakayashiki, H. Park, P
  • Organizer: Proceeding of 9^<th> Asia-Pacific Microscopy Conference(APMC9)
  • Place of Presentation: Jeju, Korea
• [Presentation] Cytological study for pathological roles of compounds analgous to AK-toxins, Disease Determinants (2008)
  • Author(s): Hosogi, N. Hyon, G-S. Osato, T. Inoue, Ikeda, K. Park, P
  • Organizer: during plant infection. Proceeding of 9^<th>Asia-Pacific Microscopy Conference(APMC9)
  • Place of Presentation: Jeju, Korea
• [Presentation] Possible roles of extracellular matrix(ECM)in phytopathgenic fungus Magnaporthe oryzae (2008)
  • Author(s): Ikeda, K. Inoue, K. Park, P
  • Organizer: Proceeding of 9^<th>Asia-Pacific Microscopy Conference(APMC9)
  • Place of Presentation: Jeju, Korea
• [Presentation] ニホンナシ花柱内における自家・他家花粉管の微細構造の解析 (2008)
  • Author(s): 河野友美、山北由貴、太田文清、朴杓允、中西テツ、安田(高崎)剛志
  • Organizer: 園芸学会、平成20年度秋季大会
  • Place of Presentation: 三重
• [Presentation] 各種糸状菌の胞子発芽過程における貯蔵物質の代謝変 (2008)
  • Author(s): 目黒紘子、井上加奈子、池田健一、朴杓允
  • Organizer: 平成20年度日本植物病理学会関西部会
  • Place of Presentation: 和歌山
• [Presentation] Sclerotinia minorの菌核形成における活性酸素種の病理学的役割(2)活性酸素種の局在 (2008)
  • Author(s): 大里友之、池田健一、朴杓允
  • Organizer: 平成20年度日本植物病理学会関西部会
  • Place of Presentation: 和歌山
• [Presentation] 灰色かび病菌におけるストロビルリン系薬剤の発芽抑制効果 (2008)
  • Author(s): 鶴見知洋、池田健一、朴杓允
  • Organizer: 平成20年度日本植物病理学会関西部会
  • Place of Presentation: 和歌山
• [Presentation] Sclerotinia minorの菌核形成における活性酸素種の病理学的役割 (2008)
  • Author(s): 大里友之、池田健一、朴杓允
  • Organizer: 平成20年度日本植物病理学会大会
  • Place of Presentation: 和歌山
• [Presentation] ナシ黒斑病菌における活性酸素種(reactive oxygenspecies ; ROS)生成遺伝子」NoxA 及びNoxB変異株の細胞学・病理学的比較調査 (2008)
  • Author(s): 玄康洙、森田雄一、池田健一、中屋敷均、朴 杓允
  • Organizer: 平成20年度日本植物病理学会
  • Place of Presentation: 和歌山
• [Presentation] いもち病菌の胞子発芽における天然栄養源の影響 (2008)
  • Author(s): 井上加奈子、池田健一、朴杓允
  • Organizer: 平成20年度日本植物病理学会
  • Place of Presentation: 松江
• [Presentation] イネ葉面フロラからのコラゲナーゼ活性を有する微生物のスクリーニング (2008)
  • Author(s): 下井沙紀、池田健一、山崎将紀、對馬誠也、朴 杓允
  • Organizer: 日本農芸化学会
  • Place of Presentation: 名古屋
• [Presentation] Sclerotinia minorの菌核形成における活性酸素種の病理学的役割 (2008)
  • Author(s): 大里友之, 池田健一, 朴杓允
  • Organizer: 平成20年度日本植物病理学会大会
  • Place of Presentation: 松江
• [Presentation] ナシ黒斑病菌における活性酸素種(reactive oxygen species ; ROS)生成遺伝子」NoxA及びNoxB変異株の細胞学・病理学的比較調査、 (2008)
  • Author(s): 玄康洙, 森田雄一, 池田健一, 中屋敷均, 朴杓允
  • Organizer: 平成20年度日本植物病理学会
  • Place of Presentation: 松江
• [Presentation] ニホンナシ花柱内における自家・他家花粉管の微細構造の解析 (2008)
  • Author(s): 河野友美, 山北由貴, 太田文清, 朴杓允, 中西テツ, 安田(高崎)剛志
  • Organizer: 園芸学会平成20年度秋季大会
  • Place of Presentation: 津
• [Presentation] Pathological roles of reactive oxygen species at penetration pegsof Alternaria altemata Japanese pear pathotype during plantinfection. (2008)
  • Author(s): Hyon, G-S., Morita, Y., Hosogi, N., Ikeda, K., Nakayashiki, H., Park, P.
  • Organizer: 9^<th> Asia-Pacific Microscopy Conference(APMC9)
  • Place of Presentation: Jeju, Korea.
• [Presentation] Cytological study for pathological roles of compounds analgous to AK-toxins, Disease Determinants, during plant infection (2008)
  • Author(s): Hosogi, N., Hyon, G-S., Osato, T., Inoue, Ikeda, K., Park, P.
  • Organizer: 9^<th> Asia-Pacific Microscopy Conference(APMC9)
  • Place of Presentation: Jeju, Korea.
• [Presentation] Possible roles of extracellular matrix (ECM) in phytopathgenic fungus Magnaporthe oryzae (2008)
  • Author(s): Ikeda, K., Inoue, K., Park, P.
  • Organizer: 9^<th> Asia-Pacific Microscopy Conference(APMC9)
  • Place of Presentation: Jeju, Korea.
• [Presentation] いもち病菌におけるハイドロフォビン(Mpgl)の接着能力への影響、 (2008)
  • Author(s): 井上加奈子, 池田健二中屋敷均, 朴杓允
  • Organizer: 第8回糸状菌分子生物学コンファレンス
  • Place of Presentation: 金沢
• [Presentation] より迅速な試料作製法 : 急速脱水 (2007)
  • Author(s): 朴杓允
  • Organizer: 医学生物学電子顕微鏡技術学会第23回学術講演会
  • Place of Presentation: 小倉
  • Year and Date: 2007-05-19
• [Presentation] 植物感染時に生じる活性酸素種の電顕定量法 (2007)
  • Author(s): 玄康洙、竹本大吾、田中愛子、BaryScott、姜山、池田健一、朴 杓允
  • Organizer: 医学生物学電子顕微鏡技術学会
  • Place of Presentation: 小倉
  • Year and Date: 2007-05-19
• [Presentation] Possible roles of the extracellular matrix(ECM)from Magnaporthe oryzae during the fungus-host adhesion (2007)
  • Author(s): Inoue,K. Ikeda,K. Park, P
  • Organizer: The4thInternational rice blast conference
  • Place of Presentation: Changsha, Hunan, China
• [Presentation] the Magnaporthe oryzae methyltransferase orthologous to Neurospora crassa Dim-2 is dispensable for the life cycle of the fungus in nature (2007)
  • Author(s): Ikeda, K. Shina, K. Kadotani, N. Tanaka, M. Murata T. Chuma I. Tosa, Y. Park, P. Mayama S. Nakayashiki, H. MoDim2
  • Organizer: 4th International rice blast conference
  • Place of Presentation: Changsha, Hunan, China
• [Presentation] ナシ黒斑病菌における活性酸素種(ROS)生成遺伝子NoxBの機能解析 (2007)
  • Author(s): 玄康洙、森田雄一、篠木武、池田健一、中屋敷均、朴杓允
  • Organizer: 平成19年度日本植物病理学会関西部会
  • Place of Presentation: 岐阜
• [Presentation] いもち病菌のシトシンDNAメチル化は同菌の生活環において必須ではない (2007)
  • Author(s): 池田健一、田中正起、村田聡樹、椎名宏太、土佐幸雄、眞山滋志、朴 杓允、中屋敷均
  • Organizer: 平成19年度日本植物病理学会関西部会
  • Place of Presentation: 岐阜
• [Presentation] いもち病菌の胞子発芽における接着能力 (2007)
  • Author(s): 井上加奈子、池田健一、朴杓允
  • Organizer: 成19年度日本植物病理学会関西部会
  • Place of Presentation: 岐阜
• [Presentation] いもち病菌感染器官の各種阻害剤処理による剥離効果 (2007)
  • Author(s): 井上加奈子、池田健一、朴杓允
  • Organizer: 平成19年度日本植物病理学会
  • Place of Presentation: 宇都宮
• [Presentation] ナシ黒斑病菌の貫穿菌糸における活性酸素種(ROS)生成遺伝子NoxAの機能解析 (2007)
  • Author(s): 玄康洙、篠木武、池田健一、中屋敷均、朴 杓允
  • Organizer: 平成19年度日本植物病理学会
  • Place of Presentation: 宇都宮
• [Presentation] いもち病菌小型分生子の病理学的役割を解明するための基礎調査 (2007)
  • Author(s): 細木直樹、中馬いづみ、篠木武、池田健一、中屋敷均、眞山滋志、土佐幸雄、朴 杓允
  • Organizer: 平成19年度日本植物病理学会
  • Place of Presentation: 宇都宮
• [Presentation] Possible roles of the extracellular matrix(ECM)from Magnaporthe oryzae during the fungus-host adhesion. (2007)
  • Author(s): Inoue, K., Ikeda, K., Park P.
  • Organizer: The 4th International rice blast conference
  • Place of Presentation: China, Changsha
• [Presentation] ナシ接種した黒星病菌の匍匐菌糸におけるぺクチナーゼ活性及び菌糸細胞壁分解に関する電顕計測解析 (2006)
  • Author(s): 姜山、朴杓允、石井英夫
  • Organizer: 平成18年度日本植物病理学会
  • Place of Presentation: 北海道
• [Presentation] 糖タンパク分解酵素によるコムギいもち病菌の感染器官の宿主粘着阻害に関する細胞学研究 (2006)
  • Author(s): 井上加奈子、姜山、細木直樹、玄康洙、兪輝星、朴 杓允、池田健一
  • Organizer: 平成18年度日本植物病理学会
  • Place of Presentation: 北海道