Diversification and transposition of avirulence genes in Magnaporthe oryzae prompted by the instability of subtelomeres
| Project/Area Number |
18380034
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Plant pathology
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| Research Institution | Kobe University |
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Principal Investigator |
TOSA Yukio Kobe University, Graduate School of Agricultural Sciences, Professor (20172158)
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| Project Period (FY) |
2006 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥17,250,000 (Direct Cost: ¥15,300,000、Indirect Cost: ¥1,950,000)
Fiscal Year 2007: ¥8,450,000 (Direct Cost: ¥6,500,000、Indirect Cost: ¥1,950,000)
Fiscal Year 2006: ¥8,800,000 (Direct Cost: ¥8,800,000)
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| Keywords | Mapnaporthe oryzae / Pyricularia orvzae / Avirulence gene |
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| Research Abstract |
AVR-Pita is an avirulence gene corresponding-to Pita, a rice gene for resistance to the rice blast fungus, Magnaporthe oryzae. Homologues of this gene are present not only in Oryza isolates, but also in Setaria isolates, Panicum isolates, Digitazia isolates, etc. We cloned these homologues with their flanking regions. A homologue from a Cenchrus isolate was not flanked by any known transposable elements. On the other hand, those from Digitaria isolates were located between two copies of a retrotransposon, Inago 1. Those from Panicum miliaceum and Setaria viridis isolates were flanked by a copy of Inago 1. Those from Oryza sativa and Setana italica isolates were accompanied by several copies of solo LTR of retrotransposons. When a dendrogram was constructed from the DNA sequences of these homologues, the homologue from the Cenchrus isolate was located at the root of the others, while those from Oryza isolates were located at a distal position.
To examine the function of these homologues, they were introduced into P2-b, an Oryza Slate virulent on rice cultivar Yashiromochi with Pita. Infection assays with these transformants revealed that the homologues from Digitazia, Panicurn and Setaria Slates maintained their function as avirulence genes. We suggest that this is because these homologues have not had a chance to interact with Pita.
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Report
(3 results)
Research Products
(13 results)
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[Journal Article] Speciation in Pyricularia inferred from multilocus phylogenetic analysis2007
Author(s)
Hirata, K, Kusaba, M, Chuma, I, Osue, J, Nakayashiki, H, Mayama, S, Tosa, Y
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Journal Title
Mycological Research 111
Pages: 799-808
NAID
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Presentation] Typing, mapping and comparative analysis of the AVR-Pita homologs in Pyricularia oryzae2007
Author(s)
Chuma, I, Ohtaka, K, Kusaba, M, Yoshida, K, Nakayashiki, H, Mayama, S, Tosa, Y, Typing
Organizer
The annual meeting of the Phytopathological Society of Japan
Place of Presentation
Utsunomiya University
Year and Date
2007-03-28
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Presentation] Dynamics of an avirulence gene locus, AVR-Pita, in Pyricularia spp2006
Author(s)
Izumi, Chuma, Chihiro, Isobe, Hiotshi, Nakayashiki, Shigeyuki, Mayama, Yukio, Tosa
Organizer
The 6th conference on Fungal Genetics and Molecular Biology
Place of Presentation
Osaka prefecture university
Year and Date
2006-11-14
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Presentation] Wandering of the avirulence gene Avr-Pita on Pyricularin chromosomes2006
Author(s)
Chuma, I, Yoshida, K, Isolie, C, Tosa, Y, Nakayashiki, H, Mayama, S
Organizer
The annual meeting of the Phytopathological Society of Japan
Place of Presentation
Sapporo Convention Center
Year and Date
2006-06-05
Description
「研究成果報告書概要(欧文)」より
Related Report
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